Article
Entomology
Jun-Qing Ge, Zhu-Hong Wang, Xi Chen, Hua Chen, Jian Huang
Summary: BmNPV p26 is conserved among all completely sequenced Lepidoptera baculoviruses and may play an important role in the virus infection cycle, with functions in both the cytoplasm and nucleus. RNAi-based knockdown of p26 decreased budded virus production in BmNPV-infected cells and may be associated with BmNPV replication during the late infection stage.
Article
Entomology
Xiong Wang, Guangyu Ma, Feifei Ren, Mian Muhammad Awais, Jingchen Sun
Summary: Research has shown that Bombyx mori fatty acid binding protein 1 (BmFABP1) is expressed in silkmoth cells and in the tissues of silkmoth larvae, where it inhibits the replication of Bombyx mori nucleopolyhedrovirus (BmNPV). This study provides new insights into the potential antiviral role of BmFABP1 and its importance in breeding BmNPV-resistant transgenic silkmoths.
Article
Biochemistry & Molecular Biology
Rui Shen, Dingding Lu, Zhijun Cao, Jinshan Huang, Yiling Zhang, Zhongyuan Shen, Xudong Tang
Summary: Inhibiting neddylation could be a strategy for combating baculoviruses, and MLN4924 has potential as a candidate drug.
ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY
(2022)
Article
Entomology
Qin Xiao, Zhan-Qi Dong, Yan Zhu, Qian Zhang, Xiu Yang, Miao Xiao, Peng Chen, Cheng Lu, Min-Hui Pan
Summary: Our study demonstrated that Bombyx mori nucleopolyhedrovirus (BmNPV) induces G2/M phase arrest in insect cells by downregulating BmCDK1 and BmCyclin B, essential for its replication. The interaction of BmNPV IAP1 with BmCDK1 was confirmed, suggesting a mechanism through which BmNPV impacts cell cycle progression. These findings enhance our understanding of virus-host interaction and provide a potential target gene linking apoptosis and the cell cycle.
Article
Biochemical Research Methods
Feifei Zhu, Dandan Song, Han Chen, Qi Tang, Shuhao Huo, Xiaoyong Liu, Keping Chen
Summary: Lipids have been suggested to play a crucial role in virus entry and infection. A comprehensive lipidomic analysis using UPLC-MS was conducted on susceptible and resistant silkworm strains, revealing key lipids associated with virus infection and immune response. The study highlights the relationship between lipid biosynthesis, viral resistance, and immune response in the host.
JOURNAL OF PROTEOME RESEARCH
(2021)
Article
Virology
Yujia Liu, Dongbin Chen, Xiaoqian Zhang, Shuqing Chen, Dehong Yang, Linmeng Tang, Xu Yang, Yaohui Wang, Xingyu Luo, Manli Wang, Zhihong Hu, Yongping Huang
Summary: This study constructed an inducible CRISPR/Cas9 system specifically targeting Bombyx mori nucleopolyhedrovirus (BmNPV), which enhanced the silkworm's resistance to the virus without affecting its economic traits.
Article
Immunology
Xu Gao, Jihai Lei, Yajie Zhu, Xi Chen, Fuxiang Mao, Meng Miao, Yanping Quan, Wei Yu
Summary: LEF3, a single-stranded DNA binding protein in Bombyx mori nucleopolyhedrovirus, can be acetylated at K18 during viral infection, affecting its nuclear localization and interaction with P143, consequently blocking viral replication.
MICROBIAL PATHOGENESIS
(2021)
Article
Microbiology
Bifang Hao, Lin Liu, Na Liu, Luping Sun, Fengxiu Fan, Jinshan Huang
Summary: In this study, we revealed that BmNPV GP64 retains its signal peptide in host cells, which is crucial for GP64 secretion across the cytomembrane. This study unveils the mechanism of signal peptide in protein targeting and provides important mechanistic understanding of BmNPV infection and host range specificity.
MICROBIOLOGY SPECTRUM
(2022)
Article
Biochemistry & Molecular Biology
Weiwei Zhou, Jindie Hong, Jinying Han, Fuchuan Cai, Qi Tang, Qian Yu, Guohui Li, Shangshang Ma, Xiaoyong Liu, Shuhao Huo, Keping Chen, Feifei Zhu
Summary: In this study, the role of glycosaminoglycan modifications, including heparan sulfate (HS) and chondroitin sulfate (CS), in Bombyx mori nuclear polyhedrosis virus (BmNPV) infection was investigated. The results showed that HS and CS are important attachment factors for BmNPV and targeting them can effectively inhibit viral infection.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Virology
Guanping Chen, Yuedong Li, Xiangshuo Kong, Shudi Zhao, Jiale Li, Xiaofeng Wu
Summary: This study found that the expression of Bombyx mori HEXIM1 (BmHEXIM1) was significantly upregulated after BmNPV infection. By suppressing BmRelish-driven immune responses, BmHEXIM1 assisted in the immune escape of BmNPV. This research provides new insights into the mechanisms of immune escape of BmNPV and theoretical guidance for the breeding of BmNPV-resistant silkworm varieties.
Article
Virology
Congyue Yao, Shijia Pan, Ying Xu, Mengze Lu, Yating Zhao, Jiayao Huo, Bifang Hao, Jinshan Huang
Summary: BmNPV is a severe pathogen that infects Bombyx mori, causing significant economic losses. This study identifies an alternative pathway for BmNPV BV assembly and release through cellular exosomal pathway, which produces virions with relatively lower infectivity.
JOURNAL OF VIROLOGY
(2023)
Article
Biochemistry & Molecular Biology
Qian Zhang, Yun-Fei Wu, Peng Chen, Tai-Hang Liu, Zhan-Qi Dong, Cheng Lu, Min-Hui Pan
Summary: Cdc37 is a molecular chaperone that plays an active role in the proliferation of BmNPV in silkworm cells. Studies have shown that Cdc37 interacts with Hsp90 to promote virus replication. Co-expression of Cdc37 and Hsp90 is more effective in enhancing virus proliferation than overexpression alone.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Zhi-meng Zhao, Hao-tong Yin, Man-man Shen, Shao-lun Zhang, Zi-kang Chen, Tao Li, Zhen-dong Zhang, Wei-guo Zhao, Xi-jie Guo, Ping Wu
Summary: This study reveals the inhibitory effect of Geldanamycin (GA) on the proliferation of Bombyx mori nuclear polyhedrosis virus (BmNPV) and uncovers the regulatory network involving miRNAs. The findings enrich our understanding of the interaction between BmNPV and silkworms, and improve our knowledge on the molecular mechanism of HSP90 inhibitors in BmNPV proliferation.
ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY
(2022)
Article
Agronomy
Xin Wang, Jun-Li Lv, Shuang Cheng, Zhi-Hao Su, Sheng Qin, Xia Sun, Xu-Dong Tang, Qiu-Ning Liu, Mu-Wang Li, Xue-Yang Wang
Summary: This study elucidates the important role of BmE74A in promoting the proliferation of BmNPV by directly interacting with the virus. The high expression of BmE74A in eggs, hemolymph, and midgut, as well as the differential expression in different resistant strains, confirms its significance in viral infection.
PEST MANAGEMENT SCIENCE
(2022)
Article
Virology
Ryuhei Kokusho, Susumu Katsuma
Summary: Some insect viruses produce occlusion bodies (OBs) to protect virions, with the p24 gene playing a crucial role in determining the shape of OBs. Mutations in p24 result in cuboidal OBs, larger in size than typical OBs, without significant impact on progeny virus production and pathogenicity.
Article
Entomology
Hiroki Takai, Kiyoshi Asaoka, Fumiko Ishizuna, Takashi Kiuchi, Susumu Katsuma, Toru Shimada
ARTHROPOD STRUCTURE & DEVELOPMENT
(2018)
Article
Biochemistry & Molecular Biology
Haokun Zhang, Takashi Kiuchi, Chikara Hirayama, Susumu Katsuma, Toru Shimada
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2018)
Article
Virology
Hiroyuki Hikida, Ryuhei Kokusho, Jun Kobayashi, Toru Shimada, Susumu Katsuma
Article
Multidisciplinary Sciences
Jung Lee, Takashi Kiuchi, Munetaka Kawamoto, Toru Shimada, Susumu Katsuma
Article
Biochemistry & Molecular Biology
Takashi Kiuchi, Yudai Sugano, Toru Shimada, Susumu Katsuma
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2019)
Article
Biochemistry & Molecular Biology
Munetaka Kawamoto, Akiya Jouraku, Atsushi Toyoda, Kakeru Yokoi, Yohei Minakuchi, Susumu Katsuma, Asao Fujiyama, Takashi Kiuchi, Kimiko Yamamoto, Toru Shimada
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2019)
Article
Virology
Keita Tsukui, Chihiro Yagisawa, Shota Fujimoto, Moe Ogawa, Ryuhei Kokusho, Mitsuyoshi Nozawa, Hideki Kawasaki, Susumu Katsuma, Masashi Iwanaga
Article
Biochemistry & Molecular Biology
Yanyan Zhou, Xiaotong Li, Susumu Katsuma, Yusong Xu, Liangen Shi, Toru Shimada, Huabing Wang
Article
Zoology
Hiroyuki Hikida, Ryuhei Kokusho, Noriko Matsuda-Imai, Susumu Katsuma
JOURNAL OF INVERTEBRATE PATHOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Jung Lee, Tomoaki Nishiyama, Shuji Shigenobu, Katsushi Yamaguchi, Yutaka Suzuki, Toru Shimada, Susumu Katsuma, Takashi Kiuchi
Summary: Samia ricini, a giant saturniid moth, has the potential to be a novel lepidopteran model species with stronger disease resistance and richer genetic resources compared to the current model species Bombyx mori. This species can be used for genetic research and forward genetic analyses, targeting specific physiological traits such as food preference, integument color, and larval spot pattern. Additionally, whole genome sequencing of S. ricini revealed its genetic similarities and differences with B. mori, as well as the expansion of certain genes in the S. ricini lineage. Causal genes for cocoon phenotypes have also been mapped, laying the groundwork for further genetic analyses.
MOLECULAR ECOLOGY RESOURCES
(2021)
Article
Biochemistry & Molecular Biology
Xiangping Dai, Takashi Kiuchi, Yanyan Zhou, Shunze Jia, Yusong Xu, Susumu Katsuma, Toru Shimada, Huabing Wang
Summary: The study conducted a comprehensive analysis of the evolution of insect beta-fructofuranosidase transferred through HGT, identifying posttransfer duplications in Lepidoptera and mutations affecting physiological function in Bombyx mori. These findings indicate that gene transfer enhances the digestive and metabolic adaptation of lepidopteran insects.
MOLECULAR BIOLOGY AND EVOLUTION
(2021)
Article
Zoology
Ryuhei Kokusho, Susumu Katsuma
Summary: The viral protein tyrosine phosphatase (PTP) and viral ecdysteroid UDP-glucosyltransferase (EGT) have been previously thought to contribute to altered host behaviors, but this study found that they may not be essential for certain abnormal behaviors in larvae induced by Bombyx mori nucleopolyhedrovirus, suggesting an unknown core mechanism of baculovirus-induced alteration of host behaviors.
JOURNAL OF INVERTEBRATE PATHOLOGY
(2021)
Article
Virology
Ryuhei Kokusho, Susumu Katsuma
Summary: Some insect viruses produce occlusion bodies (OBs) to protect virions, with the p24 gene playing a crucial role in determining the shape of OBs. Mutations in p24 result in cuboidal OBs, larger in size than typical OBs, without significant impact on progeny virus production and pathogenicity.
Article
Genetics & Heredity
Keisuke Shoji, Ryuhei Kokusho, Munetaka Kawamoto, Yutaka Suzuki, Susumu Katsuma
Summary: Baculovirus infection alters the chromatin states and gene expression of host insect cells, as shown by ChIP-seq analysis of H3K4me3 histone modification in Bombyx mori nucleopolyhedrovirus-infected cells. The data reveals genome-wide changes in euchromatic histone marks during infection progression.
Article
Biochemistry & Molecular Biology
Kenta Tomihara, Saori Tanaka, Susumu Katsuma, Toru Shimada, Jun Kobayashi, Takashi Kiuchi
Summary: In this study, two embryonic lethal mutations, l-t04 and l-m04, were discovered in semiconsomic strains T04 and M04. The mutations responsible for these lethalities were identified as the Bombyx homolog of imaginal discs arrested (Bmida) and TATA box binding protein-associated factor 5 (BmTaf5) through positional cloning and CRISPR/Cas9 mediated knockout screening. These genes were found to play important roles in late embryogenesis of B. mori.
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2023)
Article
Virology
Naiqing Xu, Xinen Tang, Xin Wang, Miao Cai, Xiaowen Liu, Xiaolong Lu, Shunlin Hu, Min Gu, Jiao Hu, Ruyi Gao, Kaituo Liu, Yu Chen, Xiufan Liu, Xiaoquan Wang
Summary: This study found that the H9N2 subtype avian influenza virus has a high airborne transmissibility, while the H7N9 virus does not. The Hemagglutinin protein of the H9N2 virus was found to play a key role in replication, stability, and airborne transmission.
Article
Virology
Samar S. Ewies, Sabry M. Tamam, Ahmed S. Abdel-Moneim, Sherin R. Rouby
Summary: Contagious ecthyma (CE) is a highly contagious viral disease of sheep and goats worldwide. The study provided a clinical description of CE and screened for genetic variation in the B2L gene. Infected sheep exhibited anorexia and oral lesions, while inoculated chicken embryos showed pock lesions. The B2L gene was successfully amplified and found to be highly conserved.
Article
Virology
Yigal Farnoushi, Dan Heller, Avishai Lublin
Summary: In recent years, new variants of avian reovirus (ARV) have caused a variety of symptoms in chickens worldwide, including viral arthritis/tenosynovitis. This study analyzed emerging ARV variants in Israel and found significant genetic diversity. Most ARV isolates in Israel belonged to genotypic cluster 5 (GC5). The study suggests that Israel has not experienced the emergence of new ARV variants since the introduction of the live vaccine (ISR-7585), but ongoing monitoring is needed due to the continuous emergence of ARV variants.
Article
Virology
Shigeru Tajima, Michiyo Kataoka, Yuki Takamatsu, Hideki Ebihara, Chang-Kweng Lim
Summary: Yokose virus (YOKV), a bat-associated flavivirus, was found to replicate at a slower rate in mosquito cells compared to other mosquito-borne flaviviruses. Specific nucleotide mutations in the virus were identified to enhance its proliferation ability in mosquito cells.
Article
Virology
Alejandra Borjabad, Baojun Dong, Wei Chao, David J. Volsky, Mary Jane Potash
Summary: This study investigated HIV brain disease using a mouse model, and found that poly I:C can reverse associated cognitive impairment and reduce virus burden. The results also revealed transcriptional changes related to neuronal function and innate immune responses.
Article
Virology
Ching-Hung Lin, Feng-Cheng Hsieh, Meilin Wang, Chieh Hsu, Hsuan-Wei Hsu, Chun-Chun Yang, Cheng-Yao Yang, Hung-Yi Wu
Summary: This study demonstrates that the synthesis of coronavirus subgenomic mRNA is not solely determined by the sequence homology between the leader TRS and TRS-B, but also by the disassociation of the coronavirus polymerase from the viral genome. This finding provides a new insight into the transcription mechanism of coronaviruses.
Article
Virology
Nicholas S. Kron, Benjamin W. Neuman, Sathish Kumar, Patricia L. Blackwelder, Dayana Vidal, Delphina Z. Walker-Phelan, Patrick D. I. Gibbs, Lynne A. Fieber, Michael C. Schmale
Summary: Two recent studies documented the genome of a novel virus in marine animals, finding that the virus is widespread in apparently healthy animals but not highly expressed in neurons. The studies also identified viral replication factories and high levels of defective genomes in chronically infected animals.
Article
Virology
Andrew M. Ramey, Laura C. Scott, Christina A. Ahlstrom, Evan J. Buck, Alison R. Williams, Mia Kim Torchetti, David E. Stallknecht, Rebecca L. Poulson
Summary: We successfully detected and characterized highly pathogenic avian influenza viruses in hunter-harvested wild waterfowl samples from western Alaska. Genomic analysis revealed three independent viral introductions into Alaska. Our findings demonstrate the utility and potential limitations of using molecular processing approaches directly on original swab samples for viral research and monitoring.
Article
Virology
Ting Gong, Dongdong Wu, Yongzhi Feng, Xing Liu, Qi Gao, Xiaoyu Zheng, Zebu Song, Heng Wang, Guihong Zhang, Lang Gong
Summary: This study discovered that quercetin can inhibit PEDV replication both in vivo and in vitro, and alleviate the clinical symptoms and intestinal injury caused by the virus. This provides a new direction for the development of PED antiviral drugs.
Article
Virology
Min Zhu, Hao Zeng, Jianqiao He, Yaohui Zhu, Pingping Wang, Jianing Guo, Jinfan Guo, Huabo Zhou, Yifeng Qin, Kang Ouyang, Zuzhang Wei, Weijian Huang, Ying Chen
Summary: The reassortment between avian H9N2 and Eurasian avian-like (EA) H1N1 viruses may have potentially changed from avian-to-mammals adaptation. This study found that the introduction of EA H1N1 internal genes into H9N2 virus restored the replication capability and resulted in extreme virulence in some cases. This raises new concerns for public health due to the possible coexistence of H9N2 and EA H1N1 viruses in dogs.
