Journal
VIROLOGY
Volume 493, Issue -, Pages 209-216Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2016.03.023
Keywords
African swine fever virus; ASFV; P1192R; DNA Topoisomerases, Type II; Topoisomerase II inhibitors; Amsacrine; Doxorubicin; Ciprofloxacin
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Funding
- Fundacao para a Ciencia e a Tecnologia (FCT) grant [PTDC/CVT/105630/2008, SFRH/BD/48654/2008]
- European Union's Seventh Framework Programme, ASFORCE [311931]
- Fundação para a Ciência e a Tecnologia [SFRH/BD/48654/2008, PTDC/CVT/105630/2008] Funding Source: FCT
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DNA topoisomerases are essential for DNA metabolism and while their role is well studied in prokaryotes and eukaryotes, it is less known for virally-encoded topoisomerases. African swine fever virus (ASFV) is a nucleo-cytoplasmic large DNA virus that infects Ornithodoros ticks and all members of the family Suidae, representing a global threat for pig husbandry with no effective vaccine nor treatment. It was recently demonstrated that ASFV codes for a type II topoisomerase, highlighting a possible target for control of the virus. In this work, the ASFV DNA topoisomerase II was expressed in Saccharomyces cerevisiae and found to efficiently decatenate kDNA and to processively relax supercoiled DNA. Optimal conditions for its activity were determined and its sensitivity to a panel of topoisomerase poisons and inhibitors was evaluated. Overall, our results provide new knowledge on viral topoisomerases and on ASFV, as well as a possible target for the control of this virus. (C) 2016 Elsevier Inc. All rights reserved.
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