Journal
VECTOR-BORNE AND ZOONOTIC DISEASES
Volume 16, Issue 11, Pages 734-736Publisher
MARY ANN LIEBERT, INC
DOI: 10.1089/vbz.2016.1948
Keywords
Dirofilaria repens; dog; microfilaremia; PCR; zoonosis
Funding
- University of Guanajuato (DAIP Project) [00086/10]
- DAIP Project [15]
Ask authors/readers for more resources
Dirofilaria repens is the causative zoonotic agent of subcutaneous dirofilariosis. Although the American continent is considered currently free of D. repens infections, in the past few years there have been some reports suggesting the presence of this agent in the Americas. In Guanajuato, Mexico, there is a report of the presence of D. repens. To determine whether this parasite is really present in Guanajuato, a total of 177 EDTA blood samples from dogs were tested using the modified Knott's technique. Using this technique, we found 15 positive samples, which were then analyzed using the PCR technique, with a panfilarial set of primers and a specific primer pair for D. repens. In one sample, the expected band was present and the corresponding amplicon, sequenced, displayed a 100% identity to D. repens, confirming the presence of this exotic and zoonotic filarial species in the municipality of Silao, Guanajuato, Mexico.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available