4.7 Letter

Pseudoparticle Neutralization Assay for Detecting Ebola-Neutralizing Antibodies in Biosafety Level 2 Settings

Journal

CLINICAL CHEMISTRY
Volume 61, Issue 6, Pages 885-886

Publisher

AMER ASSOC CLINICAL CHEMISTRY
DOI: 10.1373/clinchem.2015.238204

Keywords

-

Ask authors/readers for more resources

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.7
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

Article Nanoscience & Nanotechnology

Transparent Anti-SARS-CoV-2 and Antibacterial Silver Oxide Coatings

Mohsen Hosseini, Alex W. H. Chin, Myra D. Williams, Saeed Behzadinasab, Joseph O. Falkinham, Leo L. M. Poon, William A. Ducker

Summary: Transparent antimicrobial coatings can reduce disease transmission while maintaining surface aesthetics and functionality. Researchers fabricated a coating with silver oxide particles, which showed a significant reduction in SARS-CoV-2 and other common bacteria. These transparent antimicrobial coatings are expected to be widely used on communal touch-screens.

ACS APPLIED MATERIALS & INTERFACES (2022)

Editorial Material Biochemistry & Molecular Biology

Exploring the landscape of immune responses to influenza infection and vaccination

Sophie A. Valkenburg, Leo L. M. Poon

Summary: The antibody landscape after H3N2 vaccination is influenced by recent infections, and studying immune dynamics can help inform influenza vaccination strategies.

NATURE MEDICINE (2022)

Article Cell & Tissue Engineering

A human pluripotent stem cell-based model of SARS-CoV-2 infection reveals an ACE2-independent inflammatory activation of vascular endothelial cells through TLR4

Zhangjing Ma, Xisheng Li, Rebecca L. Y. Fan, Kevin Y. Yang, Calvin S. H. Ng, Rainbow W. H. Lau, Randolph H. L. Wong, Kevin K. Ng, Chi Chiu Wang, Peng Ye, Zelong Fu, Alex W. H. Chin, M. Y. Alison Lai, Yu Huang, Xiao Yu Tian, Leo L. M. Poon, Kathy O. Lui

Summary: The direct mechanism of SARS-CoV-2-induced endotheliitis is still unclear. This study found that human endothelial cells (ECs) have low expression of surface ACE2 and lower intracellular ACE2 compared to non-ECs in the lungs. Ectopically expressing ACE2 in hESC-ECs showed that ACE2-deficient ECs were resistant to infection but more activated than ACE2-expressing ECs. The virus induced endothelial activation by increasing monocyte adhesion, NO production, and phosphorylation of p38 MAPK, NF-kappa B, and eNOS in both ACE2-deficient and -expressing ECs. ACE2-deficient ECs responded to SARS-CoV-2 through TLR4, and its antagonist inhibited p38 MAPK/NF-kappa B/IL-1 beta activation. Single-cell RNA-seq analyses confirmed activation of the TLR4/MAPK14/RELA/IL-1 beta axis in circulating ECs of COVID-19 patients. Circulating ECs could serve as biomarkers for endotheliitis. These findings support a direct role of SARS-CoV-2 in mediating endothelial inflammation in an ACE2-dependent or -independent manner.

STEM CELL REPORTS (2022)

Article Engineering, Environmental

Evaluation of RT-qPCR Primer-Probe Sets to Inform Public Health Interventions Based on COVID-19 Sewage Tests

Xiaoqing Xu, Yu Deng, Xiawan Zheng, Shuxian Li, Jiahui Ding, Yu Yang, Hei Yin On, Rong Yang, Ho-Kwong Chui, Chung In Yau, Hein Min Tun, Alex W. H. Chin, Leo L. M. Poon, Malik Peiris, Gabriel M. Leung, Tong Zhang

Summary: Sewage surveillance is a valuable supplementary tool for COVID-19 control, and the interpretation of sewage data is crucial. This study compared the performance of seven commonly used primer-probe sets in RT-qPCR and evaluated their usefulness in a sewage surveillance program in Hong Kong. The results showed that the N1 primer-probe set was the best in detecting signals from new cases and convalescent patients in the community.

ENVIRONMENTAL SCIENCE & TECHNOLOGY (2022)

Editorial Material Infectious Diseases

Universal influenza vaccines are futile when benchmarked against seasonal influenza vaccines

Sophie A. Valkenburg, Leo L. M. Poon

LANCET INFECTIOUS DISEASES (2022)

Article Environmental Sciences

Comparison of virus concentration methods and RNA extraction methods for SARS-CoV-2 wastewater surveillance

Xiawan Zheng, Yu Deng, Xiaoqing Xu, Shuxian Li, Yulin Zhang, Jiahui Ding, Hei Yin On, Jimmy C. C. Lai, Chung In Yau, Alex W. H. Chin, Leo L. M. Poon, Hein M. Tun, Tong Zhang

Summary: Wastewater surveillance is a promising tool for monitoring the spread of COVID-19, and this study evaluated different virus concentration methods for the detection of SARS-CoV-2 in wastewater. Ultracentrifugation was found to be the most effective method, and lysis-buffer-based extraction method resulted in higher viral recovery efficiencies.

SCIENCE OF THE TOTAL ENVIRONMENT (2022)

Article Chemistry, Multidisciplinary

Effect of Surface Porosity on SARS-CoV-2 Fomite Infectivity

Mohsen Hosseini, Leo L. M. Poon, Alex W. H. Chin, William A. Ducker

Summary: By comparing porous and nonporous solids with similar chemistry, the study reveals that porosity and permeability play important roles in the infectivity of SARS-CoV-2. Access to porosity decreases infectivity, and viral DNA may become trapped in the pores. Permeability and drying time also affect the infectivity.

ACS OMEGA (2022)

Article Infectious Diseases

Development of multiplex RT-ddPCR assays for detection of SARS-CoV-2 and other common respiratory virus infections

Nathaniel K. C. Leong, Haogao Gu, Daisy Y. M. Ng, Lydia D. J. Chang, Pavithra Krishnan, Samuel S. M. Cheng, Malik Peiris, Leo L. M. Poon

Summary: Multiplex RT-ddPCR assays were developed and validated in COVID-19 cases to detect different respiratory viruses, showing their usefulness in screening for co-infections.

INFLUENZA AND OTHER RESPIRATORY VIRUSES (2023)

Article Virology

Heterosubtypic immune pressure accelerates emergence of influenza A virus escape phenotypes in mice

Julie T. S. Chu, Haogao Gu, Wanying Sun, Rebecca L. Y. Fan, John M. Nicholls, Sophie A. Valkenburg, Leo L. M. Poon

Summary: Rapid antigenic evolution of influenza A virus undermines protection from seasonal vaccines and selects for immune escape mutants. We developed an in vivo mouse model to study viral adaptation and used next generation sequencing to evaluate viral evolution in the context of broad immunity. Heterosubtypic immune pressure increased the occurrence of genome-wide mutations, resulting in the manifestation of highly virulent phenotypes that ablated vaccine mediated protection. Escape variants with high frequency mutations were found in polymerase encoding segments, suggesting the polygenic changes may contribute to the formation of these variants.

VIRUS RESEARCH (2023)

Editorial Material Microbiology

Coronavirus research: knowledge gaps and research priorities

Stanley Perlman, Malik Peiris

Summary: Decades of research on coronaviruses and studying SARS-CoV-2 during the COVID-19 pandemic have provided a great deal of knowledge, but there are still many unanswered questions and areas that need further research.

NATURE REVIEWS MICROBIOLOGY (2023)

Correction Multidisciplinary Sciences

Reconstructing antibody dynamics to estimate the risk of influenza virus infection (vol 13, 1557, 2022)

Tim K. Tsang, Ranawaka A. P. M. Perera, Vicky J. Fang, Jessica Y. Wong, Eunice Y. Shiu, Hau Chi So, Dennis K. M. Ip, J. S. Malik Peiris, Gabriel M. Leung, Benjamin J. Cowling, Simon Cauchemez

NATURE COMMUNICATIONS (2023)

Article Multidisciplinary Sciences

Within-host genetic diversity of SARS-CoV-2 lineages in unvaccinated and vaccinated individuals

Haogao Gu, Ahmed Abdul Quadeer, Pavithra Krishnan, Daisy Y. M. Ng, Lydia D. J. Chang, Gigi Y. Z. Liu, Samuel M. S. Cheng, Tommy T. Y. Lam, Malik Peiris, Matthew R. McKay, Leo L. M. Poon

Summary: Limited data is available on the genetic diversity of SARS-CoV-2 within hosts and its relationship with vaccination. The authors found that variant of Concern (VOC) samples have more sequence variations than non-VOC samples, and breakthrough infections in vaccinated individuals do not increase non-silent mutations. The study highlights the importance of understanding within-host diversity and the impact of vaccination on viral evolution.

NATURE COMMUNICATIONS (2023)

Article Biotechnology & Applied Microbiology

Hesitancy, reactogenicity and immunogenicity of the mRNA and whole-virus inactivated Covid-19 vaccines in pediatric neuromuscular diseases

Michael Kwan Leung Yu, Sophelia Hoi Shan Chan, Samuel Cheng, Daniel Leung, Sau Man Chan, Amy Suen Ka Yan, Wilfred Hing Sang Wong, Malik Peiris, Yu Lung Lau, Jaime S. Rosa Duque

Summary: Hesitancy toward COVID-19 vaccination is common for patients with neuromuscular diseases due to the lack of safety and efficacy data in this high-risk population. This study examined the factors associated with vaccine hesitancy and evaluated the reactogenicity and immunogenicity of BNT162b2 and CoronaVac vaccines in patients with neuromuscular diseases. The results showed that both vaccines were safe and effective for these patients.

HUMAN VACCINES & IMMUNOTHERAPEUTICS (2023)

Letter Immunology

Serological evidence of SARS-CoV-2 infection in dromedary camels and domestic bovids in Oman

Ihab El Masry, Salim Al Makhladi, Mohsin Al Abdwany, Afrah Al Subhi, Hatim Eltahir, Samuel Cheng, Malik Peiris, Emma Gardner, Sophie Von Dobschuetz, Baba Soumare, Madhur Dhingra, Keith Sumption, Markos Tibbo

Summary: SARS-CoV-2 infection in livestock species in Oman was investigated, and serological evidence of infection in cattle, sheep, goats, and dromedary camel was provided. Integrated data analysis of epidemiologically linked human and animal cases should be conducted to understand the extent of SARS-CoV-2 infection in animals and associated risks.

EMERGING MICROBES & INFECTIONS (2023)

Article Immunology

Impact of host genetic polymorphisms on response to inactivated influenza vaccine in children

Tim K. Tsang, Can Wang, Nicole N. Y. Tsang, Vicky J. J. Fang, Ranawaka A. P. M. Perera, J. S. Malik Peiris, Gabriel M. Leung, Benjamin J. Cowling, Dennis K. M. Ip

Summary: In randomized controlled trials, the relationship between vaccine response and host single nucleotide polymorphisms (SNPs) in 23 candidate genes was explored in 550 children who received trivalent-inactivated influenza vaccine, with adjustment for multiple testing. The GT genotype in TLR7-1817G/T (rs5741880) was associated with lower odds (OR: 0.22, 95% CI: 0.09, 0.53) of having post-vaccination hemagglutination-inhibiting (HAI) titers ≥ 40, compared to the GG and TT genotypes. Similarly, the GT genotype in TLR8-129G/C (rs3764879) was associated with lower odds (OR: 0.47; 95% CI: 0.28, 0.80) of having post-vaccination HAI titers ≥ 40, compared to the GG and AA genotypes. These findings may contribute to the development of more effective vaccines.

NPJ VACCINES (2023)

No Data Available