Article
Microbiology
Huacai Fan, Shu Li, Li Zeng, Ping He, Shengtao Xu, Tingting Bai, Yuling Huang, Zhixiang Guo, Si-Jun Zheng
Summary: Two endophytic bacterial isolates with strong antagonistic effects against TR4, identified as B. amyloliquefaciens and B. subtilis, were isolated from banana-producing areas in Yunnan Province, China. These isolates showed high inhibition rates of TR4 and promoted banana plant growth in greenhouse experiments. Molecular characterization results indicated potential biocontrol mechanisms and suggested that locally sourced antagonistic strains could serve as new biological control resources for FWB.
Article
Agronomy
Evans Were, Jochen Schone, Altus Viljoen, Frank Rasche
Summary: This study found that phenolic acids excreted by the roots of Desmodium uncinatum and Mucuna pruriens can inhibit the development and toxin synthesis of the banana wilt pathogen Foc TR4, thereby suppressing the disease.
Article
Biochemistry & Molecular Biology
Xiaofang Yang, Ronggao Gong, Yuanqi Chu, Siwen Liu, Dandan Xiang, Chunyu Li
Summary: The study revealed significant differences between the two enantiomers of prothioconazole in their activities against the virulent strain causing Fusarium wilt of banana, with R-Pro playing a major role in treatment outcomes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Tianyan Yun, Tao Jing, Dengbo Zhou, Miaoyi Zhang, Yankun Zhao, Kai Li, Xiaoping Zang, Lu Zhang, Jianghui Xie, Wei Wang
Summary: Endophytic actinomycetes show potential as a biological control resource for controlling Fusarium wilt of banana, with strain 5-4 identified as a strong antagonist against Foc TR4 and demonstrating broad-spectrum antifungal activity.
Article
Microbiology
Chen He, Zhongjun Jia, Pingshan Fan, Yunze Ruan, Ye Liang, Jingjing Ma, Jinku Li
Summary: This study found that the banana Fusarium wilt pathogen Foc TR4 has different preferences for organic and inorganic nitrogen sources. When grown on media containing inorganic nitrogen, the mycelium biomass and nitrogen content decreased significantly, while the carbon content and C:N ratio increased. The mycelium N-15 abundance significantly increased when grown on media with 50%(NH4NO3)-N-15 compared to media with 10%(NH4NO3)-N-15. These findings suggest that Foc TR4 prefers ammonium nitrogen for growth and that treating banana crops with a combination of organic and inorganic nitrogen could be a better defense against Fusarium wilt of banana.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Microbiology
Xiaxia Wang, Zhenghua Du, Chanxin Chen, Shuang Guo, Qianzhuo Mao, Wei Wu, Ruimei Wu, Wenbo Han, Peifeng Xie, Yiping Zeng, Wenna Shan, Zonghua Wang, Xiaomin Yu
Summary: In this study, a strain of Streptomyces, named XY006, was found to have strong inhibitory activity against Fusarium oxysporum causing banana wilt disease. Two cyclic lipopeptide homologs, lipopeptin A and lipopeptin B, were identified as the corresponding antifungal metabolites. Pot experiments showed that XY006 successfully colonized banana plantlets and suppressed the incidence of banana wilt disease. Furthermore, XY006 fermentation culture application improved plant growth parameters and induced peroxidase activity, indicating a potential role in inducing resistance.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Junjun Xie, Yumei Ding, Ting Gao, Shuilian He, Kai Zhao, Xuehu Yang, Jie Zhang, Zhengan Yang
Summary: This study reveals the response of C. ficifolia to FOC infection at the transcriptome and proteome levels, suggesting that FOC infection activates plant hormone signaling and transcription factors while suppressing wax biosynthesis and photosynthesis. The accumulation of oxidative-redox proteins also plays a significant role in C. ficifolia resistance to FOC.
Article
Microbiology
Raman Thangavelu, Esack Edwinraj, Muthukathan Gopi, Periyasamy Pushpakanth, Kotteswaran Sharmila, Manivasakan Prabaharan, Murugan Loganathan, Subbaraya Uma
Summary: This study identified the genomic differences and effector gene profiles of Indian Foc isolates, and developed specific molecular markers for Indian Foc R1, R4, and TR4. These markers can be used for accurate diagnosis and detection of the pathogen.
Article
Plant Sciences
R. Thangavelu, E. Edwin Raj, P. Pushpakanth, M. Loganathan, S. Uma
Summary: The study reported the draft genome of the Fusarium oxysporum f. sp. cubense TR4 strain infecting Cavendish bananas, showing differences in genome sequence compared to reference genomes.
Article
Plant Sciences
Lu Zhang, Ziyu Liu, Yong Wang, Jiaqi Zhang, Shujie Wan, Yating Huang, Tianyan Yun, Jianghui Xie, Wei Wang
Summary: Banana is an important tropical fruit crop, but banana Fusarium wilt caused by Fusarium oxysporum f. sp. cubense tropical race 4 (Foc TR4) is a highly destructive fungal disease. The use of endophytic microorganisms for biocontrol is seen as a safe and sustainable strategy. Endophytic Streptomyces strains have the potential to enhance plant resistance and promote plant growth through the production of various metabolites.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Microbiology
Luisa F. Izquierdo-Garcia, Sandra L. Carmona, Paola Zuluaga, Gustavo Rodriguez, Miguel Dita, Monica Betancourt, Mauricio Soto-Suarez
Summary: This study evaluated the biocide efficacy of 10 commercial quaternary ammonium compounds (QACs) products and one based on glutaraldehyde (GA) on Fusarium oxysporum f. sp. cubense (Foc) tropical race 4 (TR4) isolated from La Guajira, Colombia. The results showed that all QACs had 100% efficiency against reproductive structures of Foc TR4 in the absence of soil, while some QACs were still effective against survival spores with soil present. The GA-based disinfectant was able to eliminate all Foc TR4 propagules after 15 min of contact time.
Article
Biochemistry & Molecular Biology
Shuyan Li, Junpeng Ma, Shiyong Li, Fuhui Chen, Chaodong Song, Hongyan Zhang, Mingguo Jiang, Naikun Shen
Summary: Banana Fusarium wilt, caused by Fusarium oxysporum f.sp. cubense Tropical Race 4 (FOC TR4), is a serious fungal disease in East Asian banana-producing regions. Pseudomonas aeruginosa Gxun-2 has been found to significantly inhibit the growth of FOC TR4. This study investigated the response and resistance of FOC TR4 to Gxun-2 stress using comparative transcriptome analysis. The analysis revealed differentially expressed genes related to cell membrane, cell wall formation, peroxidase, ABC transporter, and autophagy that were up-regulated, while genes related to sphingolipid metabolism and chitinase were down-regulated. qRT-PCR analysis confirmed the results obtained from RNA-seq data.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
Yiting Ye, Yingying Liu, Yifei Zhang, Xin Wang, Huaping Li, Pengfei Li
Summary: Metatranscriptomic analysis reveals the presence of 20 mycoviruses in Foc, which enhances our understanding of viral diversity and taxonomy in this plant pathogen.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Agronomy
Cesar Guillen Sanchez, Philippe Tixier, Ana Tapia Fernandez, Ana Maria Conejo Barboza, Jorge A. Sandoval Fernandez, Luc de Lapeyre de Bellaire
Summary: The study found that the banana weevil quickly detected viable inoculum of Foc on external teguments and in the digestive tract after contact with infected pseudostems. External inoculum was higher than internal inoculum, but both decreased exponentially within 50 hours after contact with an infected source. Despite this, weevils remained infested for up to 2-3 days, allowing for potential inoculum dispersal.
PEST MANAGEMENT SCIENCE
(2021)
Article
Mycology
Evans Were, Jochen Schone, Altus Viljoen, Frank Rasche
Summary: This study reveals the potential mechanism of manipulating iron bioavailability in the banana rhizosphere to suppress Fusarium wilt. The results suggest that iron starvation induces the production of iron-scavenging secondary metabolites, called siderophores, by Foc TR4 as a counteractive mechanism to evade iron starvation.