Article
Medicine, Research & Experimental
Yu Zhang, Xiaoyuan Yang, Yalei Cui, Xiaobo Zhang
Summary: This study revealed that down-regulation of miR-17 in melanoma stem cells inhibits stemness and promotes differentiation by targeting ADAR2, leading to the suppression of tumor progression.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2022)
Article
Chemistry, Multidisciplinary
Huimin Zhu, Wanbo Zeng, Tangna Zhao, Weiguo Shi, Xiao Dong, Aiping Zhang, Xiang Li, Liang Xu
Summary: This study synthesized six derivatives of AICAR and evaluated their anti-fatigue effects. The optimal compound ZHM-01 exhibited significant anti-fatigue effects in animal models, while also demonstrating good biological safety.
ARABIAN JOURNAL OF CHEMISTRY
(2022)
Article
Anatomy & Morphology
Nazanin Sadat Khademi, Shirin Farivar, Masood Bazrgar, Seyedeh-Nafiseh Hassani, Najmeh Sadat Masoudi, Newsha Haghparast, Mehran Rezaei Larijani
Summary: This study investigated the cytotoxicity of three anticancer drugs on human embryonic stem cells (hESCs) and their effect on aneuploidy rate. The results showed that the drugs were non-toxic at certain concentrations and had no negative effect on the pluripotency of the cells. Aneuploid cells were dominant in both the treated and control groups.
CELLS TISSUES ORGANS
(2022)
Article
Chemistry, Physical
Xiaoguang Li, Tongkai Li, Chunhe Li, Tiejun Li
Summary: Understanding complex gene regulatory networks is a fundamental task in systems biology, and reducing the degrees of freedom and identifying main biological pathways is key. This paper applied transition path theory (TPT) and Markov state modeling (MSM) to study two cell fate decision processes, revealing important factors in determining cell fates and identifying transition rates. The results provided detailed insights into biological processes and can be applied to studying other biological systems.
JOURNAL OF CHEMICAL PHYSICS
(2021)
Article
Multidisciplinary Sciences
Aileen Julia Riesle, Meijiang Gao, Marcus Rosenblatt, Jacques Hermes, Helge Hass, Anna Gebhard, Marina Veil, Bjorn Gruening, Jens Timmer, Daria Onichtchouk
Summary: This study investigates the combinatorial mechanisms of genome activation and shows that zebrafish genome activators Pou5f3 and Nanog exhibit different roles in the activation process. They act as pioneer-like transcription factors on synergistic enhancers, promoting histone acetylation and transcription activation. However, on antagonistic enhancers, one of the factors acts as a non-pioneer-like transcription factor, competing with the activator and blocking its effects. This activator-blocker mechanism restricts widespread transcriptional activation and prevents premature expression of late developmental genes in early embryos.
NATURE COMMUNICATIONS
(2023)
Article
Medicine, Research & Experimental
Zhijuan Hua, Wenchang Yang, Dongli Li, Yixin Cui, Lu Shen, Lingna Rao, Yuxiang Zheng, Qiying Zhang, Wenyi Zeng, Yi Gong, Ling Yuan
Summary: The study found that metformin can regulate oxidative stress, angiogenesis, and fibrosis through the miR-140-3p/LIN28B/JNK/STAT3 pathway, thereby slowing the progression of subretinal fibrosis.
EXPERIMENTAL AND THERAPEUTIC MEDICINE
(2023)
Article
Cell Biology
Carolina Silva, Ilda Rodrigues, Sara Andrade, Raquel Costa, Raquel Soares
Summary: The study demonstrates that metformin can prevent vessel assembly in human microvascular endothelial cells through an AMPK-independent mechanism. It has an impact on the expression of key genes related to angiogenesis, while showing no significant effects on cell proliferation and invasion.
Article
Biochemistry & Molecular Biology
Hanuman T. Kale, Rajendra Singh Rajpurohit, Debabrata Jana, Vijay V. Vishnu, Mansi Srivastava, Preeti R. Mourya, Gunda Srinivas, P. Chandra Shekar
Summary: In this study, it was found that NANOG induces the expression of Fgfbp1 and Fgfr2 to activate ERK signaling, triggering autorepression in Nanog-high cells. Phosphorylated ERK recruits NONO to the Nanog locus to repress transcription, establishing a reciprocal NANOG-pERK regulatory circuit. This circuit regulates ERK signaling dynamics and Nanog autoregulation in pluripotent cells.
Article
Biochemistry & Molecular Biology
Arun Kumar Burramsetty, Ken Nishimura, Takumi Kishimoto, Muhammad Hamzah, Akihiro Kuno, Aya Fukuda, Koji Hisatake
Summary: This study identified and characterized proteins associated with the Nanog promoter in mouse embryonic stem cells (mESCs) using unbiased methods. The results revealed the importance of these proteins, including RNA-binding proteins (RBPs), in the maintenance of pluripotency and the reprogramming of somatic cells. The findings highlight the functional relevance of RBPs in ESC differentiation and somatic cell reprogramming.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Claudia Cano-Estrada, Lidia de Benito-Gomez, Paula Escudero-Ferruz, Neus Ontiveros, Daniel Iglesias-Serret, Jose M. Lopez
Summary: Most cancer cells increase the synthesis of purine nucleotides to support their rapid division. The use of cell culture media with physiological levels of folic acid revealed changes in purine metabolism in several human cell lines. Some cell lines accumulate the intermediate metabolite ZMP at physiological levels of folic acid, while others require artificially high levels of folic acid for ZMP accumulation. Further research is needed to understand the downstream targets of ZMP and their impact on cell function.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Engineering, Biomedical
Xiayi Xu, Qian Feng, Xun Ma, Yingrui Deng, Kunyu Zhang, Hon Son Ooi, Boguang Yang, Zhi-Yong Zhang, Bo Feng, Liming Bian
Summary: In this study, a gelatin-based structurally dynamic hydrogel was developed to emulate the intrinsic properties of the extracellular matrix. The dynamic hydrogel significantly promoted the growth and pluripotency maintenance of embryonic stem cells (ESCs).
Article
Cell & Tissue Engineering
Shuang Zhao, Chuanyu Zhang, Jia Xu, Siying Liu, Lu Yu, Shang Chen, Hang Wen, Zongjin Li, Na Liu
Summary: This study identified potential interacting proteins with Dppa3 and found that Dppa3 safeguards the stability of Uhrf1 and Nanog by inhibiting proteasome-associated degradation in ES cells.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Neurosciences
Chang Liu, Di Zhang, Zhengfang Lu, Jiang Man, Zhen Zhang, Xiaojuan Fu, Kefei Cui, Jianping Wang
Summary: This study investigated the neuroprotective mechanisms of MET in tMCAO mice and revealed its anti-apoptotic and antioxidation effects. The results showed that MET treatment improved neurological outcomes and reduced blood-brain barrier disruption and infarct volume. Additionally, MET induced anti-apoptotic activity by downregulating cleaved caspase-3 and Bax expression, and inhibited the JNK signaling pathway.
NEUROSCIENCE LETTERS
(2022)
Review
Food Science & Technology
Hafiz Abdul Khaliq, Mireille Alhouayek, Joelle Quetin-Leclercq, Giulio G. G. Muccioli
Summary: Inflammation is a defensive response of the organism to various injuries. Chronic inflammatory diseases are challenging to treat, and phytochemicals may provide an interesting alternative approach. AMP-activated protein kinase plays an important role in regulating inflammatory responses. This review summarizes studies on phytochemicals that activate AMPK to improve chronic inflammatory disease models, and highlights factors to consider when investigating AMPK in the context of phytochemicals.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2022)
Article
Medicine, Research & Experimental
Zu-Yau Lin, Ming-Lun Yeh, Ching-I. Huang, Po-Cheng Liang, Po-Yao Hsu, Shinn-Cherng Chen, Chung-Feng Huang, Jee-Fu Huang, Chia -Yen Dai, Ming -Lung Yu, Wan -Long Chuang
Summary: The advantage of combining colchicine with sorafenib or regorafenib in treating hepatocellular carcinoma (HCC) was investigated. The results showed that colchicine enhanced the anti-proliferative effects of sorafenib and regorafenib and regulated the expression of target genes and cancer stem cell markers.
BIOMEDICINE & PHARMACOTHERAPY
(2022)