Article
Chemistry, Analytical
Korakot Boonyaphon, Zhenglin Li, Sung-Jin Kim
Summary: In this study, a microfluidic recirculation system driven by water-head pressures was developed, which enables media recirculation in parallel channels with pulsatile and constant flows. The system allows control of flow rates and shear stress, and can be used for systematic and parallel analysis of cell response to fluidic shearing patterns.
ANALYTICA CHIMICA ACTA
(2022)
Article
Engineering, Biomedical
Leyla Amirifar, Amir Shamloo, Rohollah Nasiri, Natan Roberto de Barros, Ze Zhong Wang, Bige Deniz Unluturk, Alberto Libanori, Oleksandr Ievglevskyi, Sibel Emir Diltemiz, Samuel Sances, Ilangko Balasingham, Stephanie K. Seidlits, Nureddin Ashammakhi
Summary: Recent advances in biomaterials, microfabrication, microfluidics, and cell biology have led to the development of organ-on-a-chip devices that can replicate key functions of various organs. These platforms promise to provide novel insights into physiological events, including disease mechanisms, and evaluate the impact of external interventions such as drug administration. Therefore, the neuroscience field is expected to benefit greatly from these innovative tools.
Review
Biochemistry & Molecular Biology
Uyen M. N. Cao, Yuli Zhang, Julie Chen, Darren Sayson, Sangeeth Pillai, Simon D. D. Tran
Summary: Organ-on-A-chip (OoAC) devices are miniaturized in vitro constructs that aim to replicate the in vivo physiology of an organ. Different biomaterials and fabrication strategies are used to create these devices, with certain biomaterials like PDMS being preferred for their ease of fabrication. Advances in 3D printing and bioprinting techniques have allowed for the development of more complex microfluidic OoAC devices. This review evaluates the materials used for fabricating microfluidic OoAC devices and discusses the combination of additive manufacturing techniques for microfabrication.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
L. Valencia, V. Canalejas-Tejero, M. Clemente, I. Fernaud, M. Holgado, J. L. Jorcano, D. Velasco
Summary: This study introduces a novel approach to generating 3D multilayer tissue models in microfluidic platforms, successfully applied to modeling skin tissue. By developing a parallel flow method and utilizing dynamic nutrient flow conditions, deposition and maintenance of bilayer tissue were achieved, opening up the possibility for better modeling of cell interactions.
SCIENTIFIC REPORTS
(2021)
Article
Chemistry, Analytical
Chengxun Su, Yon Jin Chuah, Hong Boon Ong, Hui Min Tay, Rinkoo Dalan, Han Wei Hou
Summary: Incorporation of ECM and hydrogel in microfluidic cell culture platforms is crucial for creating a physiological cell microenvironment and establishing 3D co-culture models. The developed hydrogel patterning method based on stepped heights allows for easy adaptation in both enclosed microchannels and open surfaces, making it suitable for in-gel cultures of larger 3D cellular spheroids or microtissues.
Article
Chemistry, Analytical
Yuya Morimoto, Shogo Nagata, Miki Matsumoto, Keisuke Sugahara, Shigenori Miura, Shoji Takeuchi
Summary: The study introduces a microfluidic system for cell morphological analysis. The system consists of a microfluidic channel and a cylindrical hole for inserting cell cultures with a collagen vitrigel membrane. By applying the flow of culture medium, the alignment of cells depending on the flow direction was demonstrated, showing the potential application of the system.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Analytical
Pengcheng Li, Feiyun Cui, Heying Chen, Yao Yang, Gang Li, Hongju Mao, Xiaoyan Lyu
Summary: Macrophages and fibroblasts play crucial roles in wound healing, and studying their interrelationship is essential for understanding wound-healing mechanisms and developing drugs. This study presents a microfluidic chip designed for co-culturing these cells, with an air valve to simulate the wound-healing microenvironment. The co-culture system revealed that M2-type macrophages can promote fibroblast activation and migration, suggesting their role in facilitating the wound-healing process. This proposed microfluidic co-culture system allows non-contact cell-cell interactions and has potential significance for studying various biological processes such as wound healing, tumor microenvironment, and embryonic development.
Article
Engineering, Biomedical
Yingning He, Yue Yu, Yuqian Yang, Yexin Gu, Tianjiao Mao, Yang Shen, Qiong Liu, Ruili Liu, Jiandong Ding
Summary: This study investigates the effects of cyclic stretching on cells in a quasi-3D microenvironment, serving as a bridge between 2D and 3D systems. The researchers developed a new method to prepare an elastic membrane with topographic microstructures and integrated it into a microfluidic chip. The results show that quasi-3D cells behave differently from 2D and 3D cells in terms of cell adhesion. This study provides valuable tools for cell studies and inspires further research on the topological effects of biomaterials on cells.
BIOACTIVE MATERIALS
(2022)
Review
Pharmacology & Pharmacy
Jingyu Fu, Hailong Qiu, Cherie S. Tan
Summary: Drug discovery is a costly and complex process, and efficient methods are needed to screen potential compounds and remove toxic substances. The liver plays a crucial role in determining drug efficacy and potential side effects. The liver-on-a-chip platform based on microfluidic technology has gained attention for its ability to predict drug metabolism and hepatotoxicity, as well as study pharmacokinetics and pharmacodynamics when combined with other organ-on-chip systems.
Review
Nanoscience & Nanotechnology
Daniel Vera, Maria Garcia-Diaz, Nuria Torras, Mar Alvarez, Rosa Villa, Elena Martinez
Summary: Researchers have integrated hydrogels into microfluidic setups to develop tissue barrier-on-chips that accurately account for different cellular and acellular compartments, including the cellular components and functionality of in vivo tissues. These platforms have the potential to greatly improve the predictive capacities of in vitro systems in applications such as drug development or disease modeling, but face challenges in microfabrication.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Cell Biology
Katharina V. V. Meyer, Steffen Winkler, Pascal Lienig, Gerald Draeger, Janina Bahnemann
Summary: The use of 3D cell cultures is on the rise for drug discovery, disease modelling, and tissue engineering. New hydrogels are being developed to mimic the extracellular matrix, and the presented 3D-printed microfluidic perfusion system offers a novel solution for cultivating and evaluating four separate 3D cell cultures simultaneously. This customizable system enables easy monitoring and reduces the volume of required materials.
Review
Polymer Science
Simona Argentiere, Pietro Aleardo Siciliano, Laura Blasi
Summary: Organ-on-chip devices are the new frontier in biomedical research for producing micro-organoids and tissues for drug testing and regenerative medicine. Cell-laden microgels (CLMs) are a promising tool for 3D cell culturing and on-chip generation of micro-organs, enabling the formation of tunable 3D cellular microenvironments and long-term in vitro cultures through engineering of hydrogel matrix with balanced biochemical and biophysical cues. This review provides an overview of the recent applications of CLMs in microfluidic devices for organoids formation, highlighting their roles in OOC development and future research insights.
Review
Chemistry, Analytical
Dimple Palanilkunnathil Thomas, Jun Zhang, Nam-Trung Nguyen, Hang Thu Ta
Summary: This review covers the physiology of the human gut and the engineering approaches of gut-on-a-chip (GOC) models, including materials and fabrication, cell types, stimuli, and gut microbiota. The applications, challenges, possible solutions, and prospects for GOC models and technology are thoroughly discussed.
Article
Chemistry, Multidisciplinary
Haiqing Bai, Kristen N. Peters Olson, Ming Pan, Thomas Marshall, Hardeep Singh, Jingzhe Ma, Paige Gilbride, Yu-Chieh Yuan, Jenna McCormack, Longlong Si, Sushila Maharjan, Di Huang, Xiaohua Qian, Carol Livermore, Yu Shrike Zhang, Xin Xie
Summary: This study introduces an approach to pattern fluid guides inside microchannels by establishing differential hydrophilicity using pressure-sensitive adhesives as masks and a subsequent selective coating with a biocompatible polymer. The method achieves rapid and consistent hydrogel flow and is suitable for dynamic 3D cell culture. Additionally, the device shows potential for pharmaceutical high-throughput screening applications.
Review
Biology
Pragya Prasanna, Shweta Rathee, Vedanabhatla Rahul, Debabrata Mandal, Macherla Sharath Chandra Goud, Pardeep Yadav, Susan Hawthorne, Ankur Sharma, Piyush Kumar Gupta, Shreesh Ojha, Niraj Kumar Jha, Chiara Villa, Saurabh Kumar Jha
Summary: Alzheimer's disease is a significant global health concern with limited therapeutic options and poor understanding of its pathophysiology. Microfluidics offers new possibilities in AD research by providing insights into disease pathways and mechanisms.
Review
Chemistry, Analytical
Claudia Campanale, Silvia Galafassi, Francesca Di Pippo, Iulian Pojar, Carmine Massarelli, Vito Felice Uricchio
Summary: Biodegradable plastic mulches (BDMs) have the potential to be an eco-friendly alternative to conventional plastic mulches in agriculture. However, their long-term impacts on soil ecosystems are still uncertain, and further research is needed. Incorporating BDMs into the soil may stimulate microbial activity, but there is also a risk of incomplete degradation and release of residual microplastics and additives.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2024)
Review
Chemistry, Analytical
Xuechi Yin, Sijie Liu, Deepak Kukkar, Jianlong Wang, Daohong Zhang, Ki-Hyun Kim
Summary: The lateral flow immunoassay strip is a classic and dominant tool in point-of-care detection, known for its portability and simplicity. However, current colored nanomaterials used as signal labels in LFIA have limited sensing potential, leading to the recognition of composite nanoparticles as alternatives to enhance sensitivity, signal readout diversity, anti-matrix interference, and cost-effectiveness.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2024)
Review
Chemistry, Analytical
Yaxin Cao, Longyi Shao, Timothy P. Jones, Wenjing Deng, M. Santosh, Pengju Liu, Cheng-Xue Yang, Yaowei Li, Daizhou Zhang, Kelly Berube
Summary: The toxicity of fibrous particles in ambient air can be higher than other types of particles. Microfibers can be organic or inorganic and their presence in the atmosphere as a result of human activity raises concerns for environmental and public health. Accurate collection, identification, and understanding of the health hazards associated with these fibers are crucial for mitigation strategies and protection of public health.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2024)
Review
Chemistry, Analytical
Chenchen Xie, Chen Meng, Huilin Liu, Baoguo Sun
Summary: The development of food hazard factor (FHF) detection has been moving towards on-site inspection and mobile law enforcement, calling for simple, fast, and affordable analysis technology to ensure food safety and quality. This review comprehensively summarizes the progress in research on smartphone-assisted MIP optosensors, focusing on the key issues of accuracy and sensitivity.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2024)
Review
Chemistry, Analytical
Guillem Peris-Pastor, Cristian Azorin, Jose Grau, Juan L. Benede, Alberto Chisvert
Summary: Green Analytical Chemistry plays an important role in every stage of method development, including sample preparation. Extraction techniques have evolved from conventional ones to a variety of miniaturized techniques in order to enhance analytical performance and ensure safety for operators and the environment. Green metric tools are used to evaluate the impact and sustainability of methods, with AGREEprep selected as the most suitable tool for evaluating published methods in various applications. The miniaturization of sample preparation strategies has led to the use of less and safer solvents, reduced waste generation and energy consumption, and improved portability and throughput.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2024)
Review
Chemistry, Analytical
Helena Hruskova, Roman Reminek, Frantisek Foret
Summary: This review article summarizes the progress and recent studies in offline electromigration preparative techniques between 2018 and 2023. It covers various techniques including electrophoresis, free-flow electrophoresis, isoelectric focusing, and isotachophoresis, providing theoretical aspects, trends, and solutions to existing limitations in each technique.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2024)
Review
Chemistry, Analytical
Mayank Garg, Nicole Pamme
Summary: This review focuses on the removal strategies of templates from molecularly imprinted polymers (MIPs) used in diagnostic biosensors. Chemical-based and electrochemical-based template extraction approaches are summarized and evaluated, providing guidance for researchers in the fields of analytical chemistry, diagnostics, and materials science for the design of MIP-based sensors.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2024)
Review
Chemistry, Analytical
Ivneet Banga, Anirban Paul, Nathan Kodjo Mintah Churcher, Ruchita Mahesh Kumar, Sriram Muthukumar, Shalini Prasad
Summary: Breathomics is an emerging field that focuses on analyzing exhaled breath to diagnose and monitor respiratory diseases. Electrochemical sensors, with their high sensitivity, selectivity, and low cost, play a significant role in breathomics and can be integrated into portable devices for point-of-care diagnosis.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2024)
Review
Chemistry, Analytical
Fabjola Bilo, Paola Cirelli, Laura Borgese
Summary: This review explores the application of X-ray fluorescence (XRF) spectrometry for elemental analysis of particulate matter (PM) in air quality monitoring. It discusses the fundamentals of XRF, experimental configurations, PM sampling devices and substrate, sample preparation strategies, qualitative and quantitative analysis, as well as the challenges faced by XRF in becoming a reliable analytical technique for PM analysis.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2024)
Review
Chemistry, Analytical
Hakim Faraji
Summary: The dispersive liquid-liquid microextraction (DLLME) technique is widely popular in analytical chemistry due to its high efficiency, cost-effectiveness, and simplicity. Recent advancements have addressed the limitations of DLLME, incorporating greener solvents, innovative dispersion strategies, and simplified procedures. These changes have made DLLME a more sustainable and efficient technique.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2024)
Review
Chemistry, Analytical
Sherin F. Hammad, Inas A. Abdallah, Alaa Bedair, Reda M. Abdelhameed, Marcello Locatelli, Fotouh R. Mansour
Summary: Carbon nanomaterials (CNMs) have excellent capabilities in terms of adsorption, enhanced oxidation, and photocatalysis, attracting widespread attention in different fields. Metal-organic frameworks (MOFs) have been widely used due to their regular network structure, porous nature, and large specific surface area. However, the instability of MOFs in aqueous solutions limits their applications in chemical sensing. Recent studies have focused on increasing the stability of MOFs in water-based matrices through chemical modifications using CNMs.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2024)
Review
Chemistry, Analytical
Alexandra Pape, Oliver J. Schmitz
Summary: Low temperature plasmas based on dielectric barrier discharges have emerged as a recent development in ion source research. These ion sources are applicable to heat-sensitive analytes and can ionize a wide range of compounds. Despite intensive research on important parameters such as configuration, shape, size, and materials in the past two decades, the actual ionization mechanism for all plasma gases has not been fully elucidated. This review provides an overview of atmospheric pressure ion sources, with a focus on plasma and dielectric barrier discharge based ion sources, summarizing the reactions occurring in the plasma and discussing the applications developed by different research groups.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2024)
Review
Chemistry, Analytical
Hai Xiong, Fengli Li, Linyu Zeng, Qiyu Lei
Summary: This review provides an overview of the emerging trends in the use of functional COF nanomaterials as biosensors for DNA/RNA detection. It summarizes the construction of DNA/RNA hybridized COF nanoprobes and their potential applications in bioanalyte detection, biosensing, biomarker imaging, and clinical biomedicine.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2024)
Review
Chemistry, Analytical
Bing Yan
Summary: This article summarizes the recent progress in logic gate computing and operation based on multiple luminescence response and chemical sensing for lanthanide functionalized MOF hybrid systems. The article first describes multi-color luminescence and the subsequent multiple luminescence response. The basic concept of logic gate operation and the advantages of lanthanide-based hybrid materials are then introduced. Reports on logic gate operation using pure lanthanide MOFs are provided, followed by a detailed summary of logic gate operations using lanthanide functionalized MOF hybrid materials. Additionally, the article discusses the practical luminescent detection of analytes by correlating the luminescence responsive signals and the concentration of different analytes. Finally, the article concludes with a prospect for this topic.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2024)
Review
Chemistry, Analytical
Brij Mohan, Gurjaspreet Singh, Rakesh Kumar Gupta, Pawan Kumar Sharma, Alexander A. Solovev, Armando J. L. Pombeiro, Peng Ren
Summary: Porous materials, particularly hydrogen-bonded organic frameworks (HOFs), have gained significant attention due to their versatile applications and unique properties such as well-defined crystalline structure and easy regeneration processes. This article provides a comprehensive overview of HOFs, including synthesis methods, hydrogen bonding motifs, recent advancements, and potential applications, offering valuable guidance for further exploration in this dynamic field.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2024)