Article
Chemistry, Multidisciplinary
A. Dadashzadeh, S. Moghassemi, C. A. Amorim
Summary: This study demonstrated that PEGylated fibrin hydrogels enhanced scaffold stability, supported cell viability and proliferation. PEG:Fib 5:1 T50 showed a significantly higher cell density dynamic and a lower expression of caspase-3. In contrast, PEG:Fib 10:1 T50 hydrogels had uniform cell distribution and a higher rate of Ki67-positive cells.
MATERIALS TODAY CHEMISTRY
(2021)
Article
Cell Biology
Ali Salehi, Mohammad A. Mobarhan, Javad Mohammadi, Hosein Shahsavarani, Mohammad Ali Shokrgozar, Atefeh Alipour
Summary: The study investigated the characteristics of decellularized cabbage leaves and their potential for promoting bone differentiation, as demonstrated by enhanced osteogenic differentiation of BM-MSCs on the scaffold. These results suggest that decellularized cabbage leaves have great potential for tissue engineering applications in bone regeneration.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Engineering, Biomedical
Katsuhisa Sakaguchi, Yusuke Tobe, Jiayue Yang, Ryu-ichiro Tanaka, Kumiko Yamanaka, Jiro Ono, Tatsuya Shimizu
Summary: The article introduces a novel tissue engineering method using a net metal mould without the use of a scaffold, which allows for the formation of 3D tissues by culturing human dermal fibroblasts. The study successfully constructs scaffold-free 3D tissues with controllable strength, which may have a significant impact on next-generation tissue engineering products.
Article
Biochemistry & Molecular Biology
Nadia Nascimento da Rosa, Julia Maurer Appel, Ana Carolina Irioda, Bassam Felipe Mogharbel, Nathalia Barth de Oliveira, Maiara Carolina Perussolo, Priscila Elias Ferreira Stricker, Livia Rosa-Fernandes, Claudio Romero Farias Marinho, Katherine Athayde Teixeira de Carvalho
Summary: A 3D lung model was developed using Wharton's jelly mesenchymal stem cells (WJ-MSC) for pulmonary differentiation and bioprinting. The differentiation of WJ-MSC into lung cells and bioprinting in a 3D structure were successfully achieved, providing a promising alternative for in vitro drug testing.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Materials Science, Multidisciplinary
Zihao Jia, Yang Liu, Yingying Wang, Shiyuan Peng, Peng Jia, Wei Zhang, Xiaoyan Tan
Summary: Gas-foaming technique can transform two-dimensional scaffolds into three-dimensional scaffolds, which have shown better cartilage regeneration effects with larger pore size, higher porosity, and higher biocompatibility.
MATERIALS RESEARCH EXPRESS
(2021)
Article
Chemistry, Physical
Yujie Chen, Wei Xu, Muhammad Shafiq, Jincheng Tang, Junxiang Hao, Xianrui Xie, Zhengchao Yuan, Xianghao Xiao, Yu Liu, Xiumei Mo
Summary: This study successfully fabricated two-dimensional and three-dimensional electrospun poly(L-lactide-co-epsilon-caprolactone)/silk fibroin scaffolds with nanofibrous structure and biological function, further mimicking the microarchitecture of native cartilage through crosslinking. The three-dimensional scaffolds showed better mechanical properties, while the biocompatibility, chondrocyte proliferation, and phenotypic maintenance were superior in the three-dimensional scaffolds as well.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2021)
Review
Biotechnology & Applied Microbiology
Jiang-Nan Fu, Xing Wang, Meng Yang, You-Rong Chen, Ji-Ying Zhang, Rong-Hui Deng, Zi-Ning Zhang, Jia-Kuo Yu, Fu-Zhen Yuan
Summary: This article reviews the advances in osteochondral tissue engineering and discusses various strategies including scaffold design, material types, fabrication techniques, and functional properties. Although there are still challenges and difficulties, research in this field is of great importance for repairing OC defects.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Materials Science, Multidisciplinary
Ji Min Seok, Goeun Choe, Sang Jin Lee, Min-Ah Yoon, Kwang-Seop Kim, Jun Hee Lee, Wan Doo Kim, Jae Young Lee, Kangwon Lee, Su A. Park
Summary: The study demonstrates that using an ecofriendly mussel-inspired GO coating can effectively enhance the functionality and bioactivity of 3D-printed scaffolds. By regulating the amount of GO deposition and employing a non-toxic coating method, the surface properties are improved, aiding in promoting osteogenesis.
MATERIALS & DESIGN
(2021)
Article
Materials Science, Multidisciplinary
Pedram Iranmanesh, Mohammad Gowdini, Abbasali Khademi, Mohammad Dehghani, Mehran Latifi, Naif Alsaadi, Mehran Hemati, Ramin Mohammadi, Saeed Saber-Samandari, Davood Toghraie, Afrasyab Khan
Summary: The combination of 3D bioprinting technology with novel hydrogel and bioink has led to the successful fabrication of scaffolds with unique mechanical and biological responses. The addition of naproxen coating enhances the tensile strength of the scaffolds, showing promising compatibility with the human body.
JOURNAL OF MATERIALS RESEARCH AND TECHNOLOGY-JMR&T
(2021)
Article
Engineering, Multidisciplinary
Kun Li, Yingnan Zhang, Junwei Xu, Jingxi Wang, Xuenan Gu, Ping Li, Yubo Fan
Summary: The magnetic PCL/Fe3O4/ICA 3D scaffold, manufactured through electrospinning, exhibited enhanced cell proliferation and infiltration, especially when combined with the static magnetic field, providing a new idea for the design and application of magnetic scaffolds in bone tissue engineering.
COMPOSITES PART B-ENGINEERING
(2021)
Review
Engineering, Biomedical
Shi Hua Tan, Dun An Cliff Chua, Je Re Jeremiah Tang, Carine Bonnard, David Leavesley, Kun Liang
Summary: In vitro 3D human skin models are critical tools in advancing our understanding of skin physiology and function. Many of the existing reconstructed models are limited in terms of structure and complexity, thus failing to recapitulate native human skin. Hydrogels have been identified as useful scaffold materials for fabricating the dermal equivalent of 3D skin models, allowing for greater flexibility and control in scaffold properties and cellular incorporation. This review aims to provide a critical discussion of the biomaterial selection and design strategies in the construction of hydrogel-based full-thickness skin equivalents. At the same time, insights into future developments and technological advances which can accelerate progress in this field are offered.
ACTA BIOMATERIALIA
(2022)
Article
Medicine, Research & Experimental
Brian Chang, Ashley Cornett, Zahra Nourmohammadi, Jadan Law, Blaine Weld, Sarah J. Crotts, Scott J. Hollister, Isabelle M. A. Lombaert, David A. Zopf
Summary: The study evaluated the use of 3D-printed auricular scaffolds with and without cartilage inserts in a rodent model, showing that adding cartilage inserts can enhance clinical and histological outcomes.
Article
Engineering, Biomedical
Yazhuo Huang, Lingyu Zhang, Yongrong Ji, Hongpei Deng, Mingce Long, Shengfang Ge, Yanjie Su, Siew Yin Chan, Xian Jun Loh, Ai Zhuang, Jing Ruan
Summary: In this study, a noninvasive and intelligent monitoring scaffold was developed using carboxylated carbon nanotubes (CNTs) integrated into chemically cross-linked carboxymethyl chitosan hydrogel. The CNT scaffold exhibited improved mechanical properties, good biocompatibility, and electrochemical responsiveness. It could sensitively monitor cell differentiation degree and enhance stem cell osteogenic differentiation for new bone tissue formation.
BIOACTIVE MATERIALS
(2023)
Article
Materials Science, Multidisciplinary
Irene Chiesa, Carmelo De Maria, Giovanni Vozzi, Riccardo Gottardi
Summary: Each year, thousands of patients face life-threatening ear, nose, and throat disorders, such as tracheal stenosis, or conditions that affect their psychosocial well-being, such as microtia. Tissue engineering offers an exciting alternative by creating bioartificial constructs using three-dimensional scaffolds and human cells that can grow and develop. Bioprinting technologies, which utilize additive manufacturing, are particularly useful in addressing the complex geometries of ear, nose, and throat, allowing for the creation of patient-specific scaffolds with high design flexibility and repeatability.
Article
Materials Science, Ceramics
Amanda Maria Claro, Caroline Cassia Alves, Kelvin Sousa dos Santos, Euzane Gomes da Rocha, Marina de Lima Fontes, Gustavo Claro Monteiro, Gustavo Senra Goncalves de Carvalho, Jose Mauricio Almeida Caiut, Andrei Moroz, Sidney Jose Lima Ribeiro, Hernane S. Barud
Summary: Tissue engineering is a multidisciplinary field that aims to improve health and quality of life by restoring tissue and organ functions. Cells and scaffolds are the major components of tissue engineering, and 3D porous scaffolds are more suitable for tissue regeneration. In this study, a regenerated cellulose sponge was used as a sacrificial template to synthesize a three-dimensional porous alumina-silica scaffold.
JOURNAL OF SOL-GEL SCIENCE AND TECHNOLOGY
(2023)
Article
Engineering, Biomedical
Andrea Roberto Calore, Varun Srinivas, Linda Groenendijk, Andrada Serafim, Izabela Cristina Stancu, Arnold Wilbers, Nils Leone, Ane Albillos Sanchez, Dietmar Auhl, Carlos Mota, Katrien Bernaerts, Jules A. W. Harings, Lorenzo Moroni
Summary: This study successfully fabricated three-dimensional scaffolds with surface roughness and intra-filament open porosity using additive manufacturing combined with chemical foaming and porogen leaching. The additional porosity did not affect the elastic modulus but increased the scaffold ductility. Cells adhered and exhibited enhanced metabolic activity and secretion of osteogenic markers on the scaffolds with multiple levels of porosity.
ACTA BIOMATERIALIA
(2023)
Review
Materials Science, Multidisciplinary
Maria Camara-Torres, Pierpaolo Fucile, Ravi Sinha, Carlos Mota, Lorenzo Moroni
Summary: Bone tissue engineering utilizes additive manufacturing to create personalized 3D scaffolds that can be further functionalized to influence cell behavior.
INTERNATIONAL MATERIALS REVIEWS
(2023)
Article
Engineering, Biomedical
Daniel J. Carvalho, Anna M. Kip, Mirian Romitti, Marta Nazzari, Andreas Tegel, Matthias Stich, Christian Krause, Florian Caiment, Sabine Costagliola, Lorenzo Moroni, Stefan Giselbrecht
Summary: The function of the thyroid gland in the human body can be severely affected by endocrine disrupting chemicals (EDCs). Current in vitro assays based on 2D thyroid cell cultures are not accurate in evaluating the safety of these compounds. The development of a thyroid organoid-on-a-chip (OoC) device using polymeric membranous carriers is described in this study, which shows a high degree of functionality and represents a valuable tool for testing potential EDCs.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Engineering, Biomedical
Tianyu Yao, Honglin Chen, Rong Wang, Rebeca Rivero, Fengyu Wang, Lilian Kessels, Stijn M. Agten, Tilman M. Hackeng, Tim G. A. M. Wolfs, Daidi Fan, Matthew B. Baker, Lorenzo Moroni
Summary: This study focuses on the functionalization of scaffolds using a polymer mixing approach to immobilize VEGF-mimetic peptides. The functionalized scaffolds were validated and tested, showing potential for promoting angiogenesis and vascular tissue regeneration.
BIOACTIVE MATERIALS
(2023)
Article
Materials Science, Biomaterials
Martyna Nikody, Jiaping Li, Elizabeth Rosado Balmayor, Lorenzo Moroni, Pamela Habibovic
Summary: This article introduces a polymer-ceramic composite material that enhances its osteogenic properties by adding zinc, for the treatment of critical-sized bone defects that cannot heal spontaneously and require surgical intervention. The study found that the addition of zinc can promote the differentiation of osteogenic cells, enhance extracellular matrix production and mineralization, and demonstrate the potential for treating critical-sized bone defects.
BIOMATERIALS ADVANCES
(2023)
Article
Materials Science, Biomaterials
Clarissa Tomasina, Giorgia Montalbano, Sonia Fiorilli, Paulo Quadros, Antonio Azevedo, Catarina Coelho, Chiara Vitale-Brovarone, Sandra Camarero-Espinosa, Lorenzo Moroni
Summary: The combination of biomaterials and bioactive particles has been used to fabricate electrospun scaffolds for bone tissue engineering. Hydroxyapatite and mesoporous bioactive glasses (MBGs) are commonly used due to their osteoconductive and osteoinductive properties. However, there is limited understanding of the differences in the characteristics and performance of these particle-containing scaffolds.
BIOMATERIALS ADVANCES
(2023)
Article
Engineering, Biomedical
Juhi Chakraborty, Julia Fernandez-Perez, Kenny A. van Kampen, Subhadeep Roy, Tim ten Brink, Carlos Mota, Sourabh Ghosh, Lorenzo Moroni
Summary: Engineering biomimetic cellular microenvironments has been a priority in regenerative medicine. This study presents a 3D bioprinting approach using GelMA and SF-G bioinks to fabricate arch-like constructs for cartilage regeneration. The SF-G constructs showed higher cell proliferation and superior collagen network and chondrogenesis compared to GelMA constructs. Protein-protein interaction and gene analysis validated the in vitro results, emphasizing the role of SF-G in regulating signaling pathways. The 3D bioprinted arch-like constructs have great potential for cartilage regeneration.
Article
Engineering, Biomedical
Kevin Tabury, Emil Rehnberg, Bjorn Baselet, Sarah Baatout, Lorenzo Moroni
Summary: Bioprinting in space presents new opportunities and challenges for tissue engineering. The focus on the cardiovascular system is necessary for astronaut safety and addressing organ transplantation shortage. Recent developments in bioprinting heart tissues in space and future possibilities are discussed.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Engineering, Biomedical
Monize Caiado Decarli, Adrian Seijas-Gamardo, Francis L. C. Morgan, Paul Wieringa, Matthew B. Baker, Jorge Vicente L. Silva, Angela Maria Moraes, Lorenzo Moroni, Carlos Mota
Summary: Cartilage tissue has limited self-repair ability and irreversible progression of lesions. Tissue engineering structures, such as extrusion bioprinted constructs containing stem cell spheroids, show promise for research and therapy. In this study, a reproducible bioprinting process followed by chondrogenic differentiation using hMSC spheroids in a xanthan gum-alginate hydrogel was successfully performed. The bioprinted constructs showed stable structure, cell viability, and matrix production, demonstrating their potential as stable chondral tissue implants.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Engineering, Biomedical
Carlotta Mondadori, Amit Chandrakar, Silvia Lopa, Paul Wieringa, Giuseppe Talo, Silvia Perego, Giovanni Lombardi, Alessandra Colombini, Matteo Moretti, Lorenzo Moroni
Summary: The study investigated the ability of scaffold architecture to modulate macrophage polarization. The rhombus architecture was found to promote the release of certain cytokines by M2a macrophages, which may have a positive impact on the healing process.
BIOACTIVE MATERIALS
(2023)
Article
Engineering, Biomedical
Mariana I. Neves, Silvia J. Bidarra, Mariana V. Magalha, Ana L. Torres, Lorenzo Moroni, Cristina C. Barrias
Summary: This study reports the design of microstructured alginate hydrogels for injectable cell delivery and demonstrates their ability to guide cell morphogenesis through cellular contact guidance in a 3D setting. Alginate was modified with hydrophobic cyclooctyne groups to generate self-associative amphiphilic derivatives capable of forming microstructured hydrogels. MSCs embedded in the hydrogels were able to sense and respond to the 3D topography, regulating their shape and stress fiber organization.
MATERIALS TODAY BIO
(2023)
Article
Polymer Science
Ornella Ursini, Maddalena Grieco, Carla Sappino, Agostina Lina Capodilupo, Sara Maria Giannitelli, Emanuele Mauri, Alessio Bucciarelli, Chiara Coricciati, Valeria de Turris, Giuseppe Gigli, Lorenzo Moroni, Barbara Cortese
Summary: Bioengineered hydrogels are important platforms for studying cell behavior in tissue engineering, regenerative medicine, and in vitro disease models. This study focused on using chemically modified hyaluronic acid to create hydrogels with controllable properties for investigating cell interactions and proliferation. Rheological studies, mechanical testing, and scanning electron microscopy were conducted to determine the optimal biomechanical properties of the hydrogels. The results showed that the change in dimensionality affected cell functions and interactions.
Correction
Multidisciplinary Sciences
Jip Zonderland, Paul Wieringa, Lorenzo Moroni
Article
Biochemistry & Molecular Biology
Gabriele Addario, Daphne Eussen, Sonja Djudjaj, Peter Boor, Lorenzo Moroni, Carlos Mota
Summary: Chronic kidney disease is the twelfth leading cause of death worldwide, with limited treatment options. A new in vitro organ-on-a-chip platform using 3D printing was developed to replicate segments of the nephron, the functional unit of the kidney. This low-cost manufacturing chip features perfusable channels and mimics the renal tubulointerstitium in vitro.
MACROMOLECULAR BIOSCIENCE
(2023)
Article
Engineering, Biomedical
Kenny A. van Kampen, Elena Olaret, Izabela-Cristina Stancu, Daniela F. Duarte Campos, Horst Fischer, Carlos Mota, Lorenzo Moroni
Summary: The main function of articular cartilage is to provide a low friction surface and protect the underlying bone. In this study, a scaffold design based on hypotrochoidal curves was developed and compared to a regular woodpile design. The hypotrochoidal design showed improved mechanical properties and enhanced extracellular matrix composition, indicating its potential for cartilage tissue engineering.
MATERIALS TODAY BIO
(2023)