Article
Engineering, Environmental
Jung Seung Lee, Yi Sun Choi, Jong Seung Lee, Eun Je Jeon, Soohwan An, Min Suk Lee, Hee Seok Yang, Seung-Woo Cho
Summary: Hydrogels derived from decellularized tissue have shown huge potential in regenerative medicine, but often exhibit weak mechanical properties. In this study, a catechol-modified DT hydrogel with oxidative crosslinking chemistry was developed, showing significantly improved mechanical properties and adhesiveness for effective tissue regeneration. The modified hydrogel demonstrated enhanced osteogenic differentiation of stem cells, accelerated bone formation, and facilitated wound healing through efficient delivery of growth factors. The study supports the applicability of mechanically-reinforced, adhesive DT hydrogel for tissue regeneration.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Biotechnology & Applied Microbiology
Haobo Li, Dong Xiang, Chongcheng Gong, Xiaomin Wang, Lin Liu
Summary: A long-lasting injectable hydrogel containing EGCG and HA was prepared, which could continuously scavenge reactive oxygen species and protect ADSCs. Intra-articular injection of this hydrogel improved osteoarthritis, reduced pro-inflammatory cytokines expression, promoted cartilage repair.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Faiza Ramzan, Sobia Ekram, Trivia Frazier, Asmat Salim, Omair Anwar Mohiuddin, Irfan Khan
Summary: This study aimed to establish a protocol for the formulation of a hydrogel from decellularized human umbilical cord tissue and assess its application in the differentiation of UC-MSCs along the chondrogenic lineage. The results showed that the DUC hydrogel was compatible with MSCs and supported their chondrogenic differentiation in vitro.
BIOENGINEERING-BASEL
(2022)
Article
Engineering, Biomedical
Si Hwa Choi, Kijun Lee, Heeju Han, Hyunkyung Mo, Hyerin Jung, YoungWoo Ryu, Yoojun Nam, Yeri Alice Rim, Ji Hyeon Ju
Summary: Cartilage is a tissue with limited regeneration ability, and stem cell-based therapies have shown promise in cartilage regeneration. This study explored the potential of stem cell-derived chondrocyte ECM for cartilage regeneration. The decellularized ECM from cultured chondrocytes enhanced chondrogenesis and restored cartilage defects in a rat osteoarthritis model. The findings suggest that hiPSC-derived cartilage-like dECM could serve as a noncellular therapeutic for articu-lar cartilage reconstruction.
ACTA BIOMATERIALIA
(2023)
Review
Cell & Tissue Engineering
Wenhui Zhang, Aoling Du, Shun Liu, Mingyue Lv, Shenghua Chen
Summary: dECM and hydrogels are widely used in regenerative medicine as biocompatible materials with unique biological activity. Hydrogels, with their high water content and ability to simulate human tissue environments, are considered ideal materials for tissue engineering.
REGENERATIVE THERAPY
(2021)
Article
Biochemical Research Methods
Wenhui Chu, Gaowei Hu, Lin Peng, Wei Zhang, Zhe Ma
Summary: The study demonstrated that adCDMs, derived from antler decellularized cartilage, are rich in collagen and GAGs, with good biosafety and biocompatibility. When implanted into rabbit articular cartilage defects, adCDMs showed excellent therapeutic effects in promoting cartilage regeneration. These findings highlight the potential of adCDMs as a novel source for developing cartilage-regenerating repair materials.
JOURNAL OF BIOLOGICAL ENGINEERING
(2021)
Article
Nanoscience & Nanotechnology
Kui Wu, Yuanyuan Wang, Hong Yang, Yihuan Chen, Kunyan Lu, Yong Wu, Chunxia Liu, Haixin Zhang, Hanyu Meng, Qian Yu, Yanxia Zhang, Zhenya Shen
Summary: An injectable hydrogel based on a decellularized porcine extracellular matrix (dECM) is developed to encapsulate and deliver cardiomyocytes locally to the infarct area of the heart. This hydrogel exhibits good biocompatibility and chemotactic effects for endogenous cells and endothelial cells, improving cardiac function through promoting angiogenesis.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Polymer Science
Jing Zhou, Nier Wu, Jinshi Zeng, Ziyu Liang, Zuoliang Qi, Haiyue Jiang, Haifeng Chen, Xia Liu
Summary: This study developed a hydrogel scaffold for cartilage tissue engineering using a secondary crosslinking method. The hydrogel exhibited excellent pore structure and water absorption ability, and was non-toxic to cells. The addition of decellularized extracellular matrix and a multi-channeled structure promoted chondrogenic differentiation of adipose-derived stromal cells and engineered cartilage formation. This hydrogel scaffold shows great potential for cartilage tissue engineering.
Article
Biotechnology & Applied Microbiology
Danni Gong, Fei Yu, Meng Zhou, Wei Dong, Dan Yan, Siyi Zhang, Yan Yan, Huijing Wang, Yao Tan, Ying Chen, Bei Feng, Wei Fu, Yao Fu, Yang Lu
Summary: dcECM hydrogels derived from porcine ears through enzymatic digestion show good biocompatibility for delivering chondrocytes and forming subcutaneous cartilage in vivo. The structural and gelation kinetics of the hydrogels vary with ECM concentrations, with 10 mg/ml hydrogels supporting adhesion and proliferation of chondrocytes in vitro. In vivo, 10 mg/ml dcECM hydrogel grafts exhibit similar qualities to native cartilage, indicating their potential for tissue engineering applications in ear cartilage regeneration.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Roghayeh Najafi, Hadi Chahsetareh, Mohamad Pezeshki-Modaress, Mina Aleemardani, Sara Simorgh, Seyed Mohammad Davachi, Rafieh Alizadeh, Alimohamad Asghari, Sajad Hassanzadeh, Zohreh Bagher
Summary: Stem cell therapy combined with polycaprolactone/gelatin nanofibers and extracellular matrix (ECM) powders was used to develop composite hydrogel scaffolds for cartilage tissue engineering. The optimal composition was achieved with 4% w/v ECM powder, which showed favorable mechanical properties and cell viability. A 3-layered composite scaffold consisting of alginate sulfate/ECM and electrospun nanofibrous layers was selected as the optimum structure for cartilage regeneration based on cell viability, mechanical properties, and chondrogenesis potential.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Engineering, Biomedical
Jorke Willemse, Gilles van Tienderen, Eline van Hengel, Ivo Schurink, Diana van der Ven, Yik Kan, Petra de Ruiter, Oskar Rosmark, Gunilla Westergren-Thorsson, Kerstin Schneeberger, Bram van der Eerden, Henk Roest, Bart Spee, Luc van der Laan, Jeroen de Jonge, Monique Verstegen
Summary: This study describes the culture and expansion of human cholangiocyte organoids using liver ECM-derived hydrogels. These hydrogels support the proliferation of organoids and maintain their phenotypic characteristics. Compared to traditional mouse tumor-derived extracellular matrix, liver ECM hydrogels do not significantly alter gene or protein expression and are not affected by species-specific differences. Additionally, the use of human liver ECM extracts enables the expansion of organoids in a dynamic culture set up without the need for laborious static culture.
Article
Biotechnology & Applied Microbiology
Weiya Tang, Jun Qi, Qian Wang, Yaping Qu, Su Fu, Jie Luan
Summary: This study investigated the effects of tissue origins on the adipogenic capacity of decellularized adipose-derived matrix (DAM) by comparing it with acellular dermal matrix (ADM). The results showed that DAM could induce adipogenic differentiation and promote neovascularization and tissue remodeling in vivo. Therefore, DAM has important applications in soft tissue repair and regeneration.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Engineering, Biomedical
Liwen Zheng, Yaxian Liu, Lin Jiang, Xiaoping Wang, Yuqin Chen, Lan Li, Mingyu Song, Hongmei Zhang, Yu Shrike Zhang, Ximu Zhang
Summary: This study proposes a new strategy for pulp regeneration by using hydrogel microspheres incorporated with decellularized dental pulp matrix-derived bioactive factors to simulate a pulp-specific three-dimensional microenvironment. The constructed microspheres exhibit favorable plasticity, biocompatibility, and biological performances, and hDPSCs cultured on the microspheres demonstrate enhanced pulp-formation ability. In vivo experiments show that the microspheres facilitate the regeneration of pulp-like tissue and new dentin. The decellularized pulp matrix-derived bioactive factors mediate the multi-directional differentiation of hDPSCs to regenerate the pulp tissue.
ACTA BIOMATERIALIA
(2023)
Article
Biotechnology & Applied Microbiology
Marco A. Herrera Quijano, Nadia Sharma, Pascal Morissette Martin, Cheryle A. Seguin, Lauren E. Flynn
Summary: This study developed and characterized a cell culture platform incorporating decellularized nucleus pulposus (DNP) for tissue regeneration and cell differentiation. The results showed that the 2-D coatings derived from DNP could support cell attachment and growth, but did not maintain or rescue the phenotype of primary cells. Similarly, the incorporation of DNP particles within the 3-D hydrogels was insufficient to maintain or rescue changes in cell phenotype associated with extended in vitro culture.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Nanoscience & Nanotechnology
Chengqi Lyu, Chi Cheng, YuShi He, Ling Qiu, Zijun He, Derong Zou, Dan Li, Jiayu Lu
Summary: Porous scaffolds are commonly used for cartilage tissue regeneration, but understanding the impact of their hierarchical structure on the regeneration process can be challenging. In this study, a graphene hydrogel with stable and adjustable structure was used as a model scaffold to investigate the effect of porous structure on matrix remodeling and chondrocyte ingrowth. The results showed that the graphene scaffold with an open pore structure accelerated and balanced cartilage remodeling. Furthermore, this enhanced remodeling selectively promoted the expression of collagen type II fibrils, providing new insights into scaffold design for cartilage repair.
ACS APPLIED MATERIALS & INTERFACES
(2022)