Article
Nutrition & Dietetics
Wouter Vankrunkelsven, Jan Gunst, Karin Amrein, Danielle E. Bear, Mette M. Berger, Kenneth B. Christopher, Valentin Fuhrmann, Michael Hiesmayr, Carole Ichai, Stephan M. Jakob, Sigismond Lasocki, Juan C. Montejo, Heleen M. Oudemans-van Straeten, Jean-Charles Preiser, Annika Reintam Blaser, Anne-Francoise Rousseau, Pierre Singer, Joel Starkopf, Arthur R. van Zanten, Steffen Weber-Carstens, Jan Wernerman, Alexander Wilmer, Michael P. Casaer
Summary: The survey revealed a wide variation in current practices of micronutrient, phosphate and magnesium measurement and parenteral administration, suggesting a risk of insufficient prevention, diagnosis and treatment of deficiencies. These results provide the context for future comparative studies, and identify areas for knowledge translation and recommendations.
CLINICAL NUTRITION
(2021)
Article
Respiratory System
Cecilia Lopez-Martinez, Paula Martin-Vicente, Juan Gomez de Ona, Ines Lopez-Alonso, Helena Gil-Pena, Elias Cuesta-Llavona, Margarita Fernandez-Rodriguez, Irene Crespo, Estefania Salgado del Riego, Raquel Rodriguez-Garcia, Diego Parra, Javier Fernandez, Javier Rodriguez-Carrio, Francisco Jose Jimeno-Demuth, Alberto Davalos, Luis A. Chapado, Eliecer Coto, Guillermo M. Albaiceta, Laura Amado-Rodriguez
Summary: In this study, researchers identified two transcriptomic clusters in critically ill COVID-19 patients based on peripheral blood transcriptomes. One cluster was characterized by interferon-related expression and the other promoted B-cell activation. These clusters had different ICU outcomes, suggesting different pathogenetic mechanisms. The findings demonstrate the potential of transcriptomics in identifying patient endotypes for personalized therapies in severe COVID-19.
EUROPEAN RESPIRATORY JOURNAL
(2023)
Review
Chemistry, Analytical
Guanghui Niu, Alexander Knodel, Sebastian Burhenn, Sebastian Brandt, Joachim Franzke
Summary: The development of miniature, sensitive, high throughput, and in-situ analytical instruments using dielectric barrier discharge (DBD) has become a hot topic in modern analytical chemistry. DBD offers advantages such as easy operation, simple configuration, ambient working temperature and pressure, low power consumption, and miniature dimension. Its applications in analytical atomic spectrometry include serving as excitation or atomization sources in AES, AAS, and AFS, as well as functioning as a vapor generation system.
ANALYTICA CHIMICA ACTA
(2021)
Review
Food Science & Technology
Jorgelina Zaldarriaga Heredia, Marcelo Wagner, Florencia Cora Jofre, Marianela Savio, Silvana Mariela Azcarate, Jose Manuel Camina
Summary: Food products, especially those with high value-added, undergo strict quality controls to attest to their specific features. The use of multi-element composition analysis combined with chemometrics has proven to be an effective method for food authentication and quality control. This review provides an overview of the state-of-the-art in this field, highlighting the characteristics and strengths and weaknesses of applied analytical techniques.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2023)
Article
Cell & Tissue Engineering
Seyed-Mohammad Reza Hashemian, Rasoul Aliannejad, Morteza Zarrabi, Masoud Soleimani, Massoud Vosough, Seyedeh-Esmat Hosseini, Hamed Hossieni, Saeid Heidari Keshel, Zeinab Naderpour, Ensiyeh Hajizadeh-Saffar, Elham Shajareh, Hamidreza Jamaati, Mina Soufi-Zomorrod, Naghmeh Khavandgar, Hediyeh Alemi, Aliasghar Karimi, Neda Pak, Negin Hossieni Rouzbahani, Masoumeh Nouri, Majid Sorouri, Ladan Kashani, Hoda Madani, Nasser Aghdami, Mohammad Vasei, Hossein Baharvand
Summary: This study investigated the safety and effectiveness of multiple high-dose infusions of MSCs derived from placenta and umbilical cord in critically ill COVID-19-induced ARDS patients. Some patients showed significant improvement in respiratory distress and reduction in inflammatory markers after cell infusions.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Medicine, General & Internal
Friederike S. Schuster, Peter Nyvlt, Patrick Heeren, Claudia Spies, Moritz F. Adam, Thomas Schenk, Frank M. Brunkhorst, Gritta Janka, Paul La Rosee, Cornelia Lachmann, Gunnar Lachmann
Summary: This study systematically investigated the differential diagnoses of hyperferritinemia in critically ill adult patients without HLH. It found that sepsis or septic shock, liver disease (except hepatitis), and hematological malignancy are important differential diagnoses. Therefore, quick investigation of differential diagnoses is necessary in hyperferritinemic patients.
JOURNAL OF CLINICAL MEDICINE
(2023)
Review
Biochemistry & Molecular Biology
Marcela Kanova, Pavel Kohout
Summary: Tryptophan is an essential amino acid with key roles in physiological processes, and its metabolism is crucial in critically ill patients. Modulation of tryptophan metabolism is important for preventing and treating inflammation-related diseases and complications in critically ill patients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Critical Care Medicine
Jenna Spring, Laveena Munshi
Summary: This review provides diagnostic considerations and management approaches for common benign hematologic emergencies, including thrombotic thrombocytopenic purpura, atypical hemolytic uremic syndrome, disseminated intravascular coagulopathy, catastrophic antiphospholipid antibody syndrome, hemophagocytic lymphohistiocytosis, acute chest syndrome associated with sickle cell disease, and hyperhemolysis syndrome.
Article
Pediatrics
Nadir Yehya, Robinder G. Khemani, Simon Erickson, Lincoln S. Smith, Courtney M. Rowan, Philippe Jouvet, Doug F. Willson, Ira M. Cheifetz, Shan Ward, Neal J. Thomas
Summary: This study aimed to develop an operational and evidence-based definition of respiratory dysfunction for critically ill children through a comprehensive literature review.
Article
Medicine, General & Internal
Matthew W. Semler, Jonathan D. Casey, Bradley D. Lloyd, Pamela G. Hastings, Margaret A. Hays, Joanna L. Stollings, Kevin G. Buell, John H. Brems, Edward T. Qian, Kevin P. Seitz, Li Wang, Christopher J. Lindsell, Robert E. Freundlich, Jonathan P. Wanderer, Jin H. Han, Gordon R. Bernard, Wesley H. Self, Todd W. Rice
Summary: The study found that using different oxygen saturation targets in critically ill adults receiving invasive mechanical ventilation did not affect the number of ventilator-free days or in-hospital mortality rates.
NEW ENGLAND JOURNAL OF MEDICINE
(2022)
Article
Immunology
Muhammad Z. Mushtaq, Saad B. Z. Mahmood, Aysha Almas, Syed Ather Wasti, Syed Ahsan Ali
Summary: This study examined the response of tocilizumab in COVID-19 patients by analyzing clinical parameters and inflammatory markers. The results showed that tocilizumab can decrease the need for mechanical ventilation and improve inflammatory markers and neutrophil to lymphocyte ratio. However, it is associated with adverse events including bacterial and fungal infections.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2022)
Review
Nutrition & Dietetics
Annika Reintam Blaser, Jan Gunst, Carole Ichai, Michael P. Casaer, Carina Benstoem, Guillaume Besch, Stephane Dauger, Sonja M. Fruhwald, Michael Hiesmayr, Olivier Joannes-Boyau, Manu L. N. G. Malbrain, Maria-Helena Perez, Stefan J. Schaller, Angelique de Man, Joel Starkopf, Kadri Tamme, Jan Wernerman, Mette M. Berger
Summary: This systematic review identified significant variations in the prevalence of hypophosphatemia among critically ill patients, with its association with adverse outcomes such as prolonged hospital stay, need for respiratory support, and higher mortality. However, there is insufficient evidence regarding the optimal threshold for critical hypophosphatemia, frequency of phosphate measurements, and timing for correcting hypophosphatemia.
CLINICAL NUTRITION
(2021)
Article
Biochemistry & Molecular Biology
Daniel Mota-Rojas, Dina Villanueva-Garcia, Alfonso Solimano, Ramon Muns, Daniel Ibarra-Rios, Andrea Mota-Reyes
Summary: Perinatal asphyxia is a pathological condition caused by inadequate oxygen supply to internal organs, leading to hypoxic-ischemic encephalopathy as its clinical manifestation. The search for biomarkers and treatment strategies to predict the severity of lesions is ongoing, with hypothermia proving to be an effective neuroprotective strategy.
Article
Veterinary Sciences
Eleonora Gori, Alessio Pierini, Erica Bartolomeo, Gianila Ceccherini, Anna Pasquini, Veronica Marchetti
Summary: This study compared serum total thyroxine concentrations in hospitalized critical cats and non-hospitalized cats with non-thyroidal chronic diseases, finding that tT4 was associated with mortality in critical cats.
VETERINARY SCIENCES
(2021)
Article
Reproductive Biology
M. Ider, A. Naseri, M. Ok, E. Gulersoy, T. M. Bas, K. Uney, T. M. Parlak, A. Abdelaziz
Summary: The objective of this study was to evaluate the role of biomarkers specific to lung endothelial and epithelial damage in determining lung injury and its severity in calves with perinatal asphyxia. The study also aimed to assess the prognostic importance of these biomarkers among survivors and non-survivors. The results showed that serum concentrations of sRAGE and sE-selectin played a significant role in determining lung injury, and pH, pCO(2), lactate, HCO3, BE, and respiratory rate were useful indicators for predicting mortality.
Article
Agriculture, Dairy & Animal Science
Giuseppe Sarli, Carolina Castagnetti, Carlo Bianco, Giulia Ballotta, Giorgia Tura, Martina Caporaletti, Marco Cunto, Giancarlo Avallone, Cinzia Benazzi, Fabio Ostanello, Daniele Zambelli
Summary: This study highlights the association between placenta injuries and outcome conditions in newborn puppies, confirming the usefulness of histological placenta investigation in evaluating newborn health and prognosis. The function of the placenta is crucial for fetal development, and its impairment can lead to negative outcomes.
Article
Veterinary Sciences
Chiara Del Prete, Francesca Freccero, Aliai Lanci, Gayle D. Hallowell, Chiara Bullone, Carolina Castagnetti, Maria Pia Pasolini
Summary: The study evaluated the feasibility and reliability of an ultrasonographic technique for measuring Ao and CVC in healthy neonatal foals, with the left ventral view showing the highest reliability. Additionally, a positive correlation between bodyweight and CVC measurement, as well as age and CVC measurements and ratios, was found.
VETERINARY MEDICINE AND SCIENCE
(2021)
Article
Reproductive Biology
Aliai Lanci, Laura Ingra, Francesco Dondi, Federico Tomasello, Gabriella Teti, Jole Mariella, Mirella Falconi, Carolina Castagnetti
Summary: This study described the morphology of equine amniotic fluid and amniotic membrane using light and electron microscopy for the first time, revealing the composition of cells and structures. This could contribute to a better understanding of the changes in AF cells during pregnancy and the potential role of AM as a secondary source of pulmonary surfactant in horses.
Article
Agriculture, Dairy & Animal Science
Aliai Lanci, Beatrice Benedetti, Francesca Freccero, Carolina Castagnetti, Jole Mariella, Johannes P. A. M. van Loon, Barbara Padalino
Summary: This study developed and evaluated a composite pain scale for foals, which proved to be a valid tool to assess pain and quality of life in foals. However, further modifications and validation are needed.
Article
Agriculture, Dairy & Animal Science
Aliai Lanci, Jole Mariella, Nicola Ellero, Alice Faoro, Tanja Peric, Alberto Prandi, Francesca Freccero, Carolina Castagnetti
Summary: The study aimed to evaluate hormone concentrations in the hair of mares and foals in relation to their clinical parameters. The results showed that mare's endocrine setting influences hormone concentrations in foal's hair, and foals with different clinical conditions have different hormone concentrations. Furthermore, hormone concentrations in the hair of mares hospitalized for attended parturition did not differ from those at the breeding farm.
Article
Biochemistry & Molecular Biology
Gloria Isani, Alberto Niccolai, Giulia Andreani, Thomas Dalmonte, Elisa Bellei, Martina Bertocchi, Mario R. Tredici, Liliana Rodolfi
Summary: This study investigated the effects of varying iron concentrations on the biochemical composition and iron bioaccumulation in cyanobacteria. The results revealed a close relationship between iron concentration in the culture media and the iron content in the biomass. The study also investigated the iron speciation and the role of C-phycocyanin as an iron binding protein.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Agriculture, Dairy & Animal Science
Aliai Lanci, Francesca Perina, Andre Donadoni, Carolina Castagnetti, Jole Mariella
Summary: This study retrospectively describes the incidence, causes, severity categories, resolution procedures, and postpartum complications of dystocia in hospitalized Standardbred mares. It also evaluates the effects of dystocia on the clinical and blood parameters of their foals. The incidence of dystocia was 4.9% and it was associated with longer stage II and increased occurrences of postpartum complications and neonatal diseases.
Article
Agriculture, Dairy & Animal Science
Giulia Andreani, Thomas Dalmonte, Alessandro Guerrini, Caterina Lupini, Micaela Fabbri, Enea Ferlizza, Gloria Isani
Summary: This study investigated the effects of dietary supplementation with Boswellia serrata and Salix alba extracts on serum and albumen proteins, zinc and iron, and yolk cholesterol content in Leghorn hens during the early laying phase. The results showed no significant differences between the control and supplemented hens for the analyzed biochemical indices. Moreover, the supplementation with phytoextracts did not negatively affect the physiological variations in serum proteins, suggesting that it can be safely used as a treatment to prevent inflammatory states during the onset of laying.
Article
Biochemistry & Molecular Biology
Gloria Isani, Enea Ferlizza, Martina Bertocchi, Thomas Dalmonte, Simonetta Menotta, Giorgio Fedrizzi, Giulia Andreani
Summary: This research analyzed the iron content and speciation in commercial samples of Arthrospira spp., or blue-green algae, sold as food supplements. The study found that iron in most samples was mainly bound to high molecular mass ligands, but in four samples it was also bound to low molecular mass ligands. Phycocyanin was identified as the principal iron-binding protein in the commercial samples.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Veterinary Sciences
Thomas Dalmonte, Carla Giuditta Vecchiato, Giacomo Biagi, Micaela Fabbri, Giulia Andreani, Gloria Isani
Summary: In recent years, the microalga market has grown due to its interesting nutritional profile, and it is important to study how different species of microalgae can be used in animal nutrition. This study investigated the iron content in four microalgae and found that Chlorella vulgaris could be a valuable iron supplementation in dog nutrition.
VETERINARY SCIENCES
(2023)
Article
Nutrition & Dietetics
Simona Parisse, Alessandra Gianoncelli, Gloria Isani, Francesco Luigi Gambaro, Giulia Andreani, Emil Malucelli, Giuliana Aquilanti, Ilaria Carlomagno, Raffaella Carletti, Monica Mischitelli, Flaminia Ferri, Veronica Paterna, Quirino Lai, Gianluca Mennini, Fabio Melandro, Cira Di Gioia, Massimo Rossi, Stefano Iotti, Michela Fratini, Stefano Ginanni Corradini
Summary: This study aimed to evaluate the magnesium content in human cirrhotic liver and its correlation with serum AST levels, expression of hepatocellular injury, and MELDNa prognostic score. The results showed that patients with cirrhosis had lower hepatic magnesium content and higher percentage of TRPM7-positive hepatocytes. The increase in hepatic magnesium content and TRPM7 expression were associated with worsening of MELDNa score. These findings suggest that severity of hepatocyte injury and prognosis in cirrhosis are closely related to magnesium depletion and overexpression of TRPM7.
Article
Veterinary Sciences
Nicola Ellero, Aliai Lanci, Vito Antonio Baldassarro, Giuseppe Alastra, Jole Mariella, Maura Cescatti, Luciana Giardino, Carolina Castagnetti
Summary: The end of pregnancy and the birth bring significant changes to the fetus, requiring adaptation to the extra-uterine environment. Trophic factors and thyroid hormones play important roles in the final maturation of the fetus. This study evaluates trophic factors and thyroid hormone levels in healthy mares and foals during parturition and the first 72 hours of life. The results demonstrate a decrease in trophic factors in foal plasma over time and a correlation between their levels. Trophic factors are expressed in placental tissues and are crucial for fetal development and adaptation to extra-uterine life.
VETERINARY SCIENCES
(2022)
Article
Veterinary Sciences
Nicola Ellero, Aliai Lanci, Vito Antonio Baldassarro, Giuseppe Alastra, Jole Mariella, Maura Cescatti, Carolina Castagnetti, Luciana Giardino
Summary: Neonatal Encephalopathy is a term used to describe non-infectious neurological signs in newborn foals, and understanding the pathophysiology of this condition is important for diagnosis. This study aimed to characterize a population of affected foals and evaluate the levels of trophic factors and thyroid hormones. The results suggest that the dysregulation of trophic factor expression and their relationship with thyroid hormones may play a role in the mechanisms of Neonatal Encephalopathy.
VETERINARY SCIENCES
(2022)
Article
Veterinary Sciences
Alessandro Guerrini, Thomas Dalmonte, Caterina Lupini, Giulia Andreani, Roberta Salaroli, Giulia Quaglia, Augusta Zannoni, Maurizio Scozzoli, Monica Forni, Gloria Isani
Summary: This study evaluated the safety and effects of supplementing Leghorn hens' diet with Boswellia serrata and Salix alba. The supplementation was found to increase water consumption, decrease egg weight, and have a sedative effect on the hens.
VETERINARY SCIENCES
(2022)
Article
Veterinary Sciences
Aliai Lanci, Martina Ingallinesi, Maria Morini, Francesca Freccero, Carolina Castagnetti, Jole Mariella
Summary: Hydramnios is a rare condition in mares, often associated with fetal anomalies. The accumulation of excessive fluid within the amniotic compartment can predispose to the rupture of the prepubic tendon. Treatment approaches may vary, with some authors suggesting induction of parturition and others opting for conservative methods.
VETERINARY SCIENCES
(2021)
Article
Reproductive Biology
Haolin Li, Xiaomeng Pei, Hao Yu, Wei Wang, Dagan Mao
Summary: This study investigated the effects of APN/AdipoRon on autophagy and apoptosis in goat luteal cells, and found that autophagy and apoptosis play important roles in the structural regression of goat corpus luteum.
Article
Reproductive Biology
Yan-Qiu Wang, He-Xuan Qu, Yan-Wei Dong, Jia-Jia Qi, Hua-Kai Wei, Hao Sun, Hao Jiang, Jia-Bao Zhang, Bo-Xing Sun, Shuang Liang
Summary: This research reveals the crucial role of Ferroptosis Suppressor Protein 1 (FSP1) in regulating the development and quality of porcine early embryos. Inhibition of FSP1 impairs blastocyst formation, induces glutathione-independent ferroptosis, and leads to oxidative stress due to mitochondrial dysfunction.
Article
Reproductive Biology
Xiong Lai, Ruizhuo Liu, Mengyu Li, Yaochun Fan, Hongxia Li, Guotao Han, Ruijie Guo, Hairui Ma, Huimin Su, Wanjin Xing
Summary: This study identifies WDR54 as a protein involved in sperm-oocyte fertilization and shows that it interacts with IZUMO1 and JUNO. The formation of the JWIJ complex on the oocyte surface suggests its importance in sperm-oocyte adhesion and fusion. This discovery provides new insights into the mechanisms of mammalian sperm-oocyte adhesion and fusion.
Article
Reproductive Biology
S. Nandi, B. Sampath Kumar, P. S. P. Gupta, S. Mondal, V. Girish Kumar
Summary: This study examined the effects of plant bioactive compounds on the survival and growth parameters of ovine preantral follicles granulosa cells (GCs) and the expression of key developmental genes. The results showed that KAE, MYR and QUE had dose-dependent responses on the morphological and functional parameters of the cells, with KAE being the most potent in augmenting ovarian functions.
Article
Reproductive Biology
Francisco E. Martin-Cano, Gemma Gaitskell-Phillips, Laura Becerro-Rey, Eva da Silva, Javier Masot, Eloy Redondo, Antonio Silva-Rodriguez, Cristina Ortega-Ferrusola, Maria Cruz Gil, Fernando J. Pena
Summary: The presence of a more efficient glycolysis mechanism depending on pyruvate in stallion spermatozoa can counteract the detrimental effects of higher glucose concentrations. Incubating spermatozoa with 10 mM pyruvate in a modified Tyrode's media with 67 mM glucose showed increased motility and maintained viability. This improvement is attributed to the conversion of lactate to pyruvate and increased NAD(+) enhancing glycolysis efficiency.
Article
Reproductive Biology
Thanida Sananmuang, Denis Puthier, Catherine Nguyen, Kaj Chokeshaiusaha
Summary: Ongoing progress in mRNA-Sequencing technologies has contributed to the refinement of assisted reproductive technologies. This study utilized mRNA-Seq datasets of various mammalian species to analyze differential transcript usage (DTU) and identified ten cross-species DTU genes. Gene Ontology (GO) term enrichment analysis revealed the involvement of these genes in RNA and cell-cycle control mechanisms, suggesting their importance during oocyte maturation. Further exploration of these genes' transcript isoforms may lead to the discovery of novel markers for reproductive technologies.
Article
Reproductive Biology
Chenglei Song, Zhe Zhang, Yilin Wei, Yaqing Dou, Kunlong Qi, Xiuling Li, Feng Yang, Xinjian Li, Kejun Wang, Ruimin Qiao, Xuelei Han
Summary: In this study, the changes in protein levels in boar sperm with different liquid preservation abilities were analyzed. Key proteins related to sperm liquid preservation ability were identified. The findings provide new insights into the molecular mechanisms of boar sperm liquid preservation ability.
Article
Reproductive Biology
Genevieve M. VanWye, Emily G. Smith, Christine Spinka, Michael F. Smith, Matthew C. Lucy, Jordan M. Thomas
Summary: A series of experiments evaluated treatment schedules for controlling the estrous cycle in beef heifers. The results showed that the duration of progestin treatment and the timing of prostaglandin administration had an impact on estrus expression and pregnancy rates.
Article
Reproductive Biology
Hongwei Duan, Shuai Yang, Longfei Xiao, Shanshan Yang, Zhenxing Yan, Fang Wang, Xiaofei Ma, Lihong Zhang, Yong Zhang, Junjie Hu, Xingxu Zhao
Summary: This study first elucidated the mechanism by which melatonin regulates progesterone secretion in the corpus luteum of sheep, namely by promoting progesterone secretion through the regulation of autophagy. This has important implications for further exploring the role of melatonin in regulating sheep ovarian function.
Article
Reproductive Biology
Zhijie Zheng, Yingfang Guo, Yonghui Zheng, Haichong Wu
Summary: This study demonstrates that miR-92b plays a protective role in LTA-induced endometritis by targeting FZD10 and reducing the production of pro-inflammatory cytokines, thus alleviating uterine injury.
Article
Reproductive Biology
Areeg Almubarak, Sanghoon Lee, Il-Jeoung Yu, Yubyeol Jeon
Summary: This study found that Nobiletin (NOB) can promote the cryosurvival and embryo development of porcine sperm after in vitro fertilization (IVF). The addition of NOB improves sperm motility, acrosome integrity, viability, and mitochondrial membrane potential, reduces lipid peroxidation, and increases cleavage and blastocyst formation rates. Furthermore, NOB treatment upregulates the expression of genes related to pluripotency and cell differentiation in the resulting embryos.
Article
Reproductive Biology
Yuan Li, Lishu Li, Wenjie Xiong, Xing Duan, Huaming Xi
Summary: This study investigated the cytotoxicity and toxicological mechanisms of fluorochloridone (FLC) on cultured goat Sertoli cells. The results showed that FLC exposure induced oxidative stress, promoted apoptosis and autophagy, inhibited lysosomal biogenesis, and blocked autophagic flux in goat Sertoli cells. FLC also caused mitochondrial dysfunction and disturbed mitophagy, leading to cell apoptosis. The antioxidant N-acetyl-L-cysteine (NAC) reduced FLC-induced ROS accumulation and reversed the disorder of autophagy levels.
Article
Reproductive Biology
Gabriela Ramos Leal, Thais de Almeida Oliveira, Mariana Pedrosa de Paula Guimaraes, Lucas Francisco Leodido Correia, Erlandia Marcia Vasconcelos, Joanna Maria Goncalves Souza-Fabjan
Summary: This study investigated the time course of lipid accumulation during IVM and the effects of lipid modulators on cat oocytes. The results showed that adding lipid modulators can reduce lipid content and improve viability after cryopreservation.
Article
Reproductive Biology
Isabella Rio Feltrin, Amanda Guimaraes da Silva, Cecilia Constantino Rocha, Priscila Assis Ferraz, Paola Maria da Silva Rosa, Thiago Martins, Juliano Coelho da Silveira, Milena Lopes Oliveira, Mario Binelli, Guilherme Pugliesi, Claudia Maria Bertan Membrive
Summary: The study evaluated the effects of E2 treatment on Nelore heifers on day 15 of the estrous cycle, finding that E2 promoted an increase in PGFM concentrations and accelerated functional and structural luteolysis by upregulating the expression of PGR and OXTR. This suggests that within 3 hours after E2 stimulus, the expression of these receptors is associated with triggering luteolysis in cattle.
Article
Reproductive Biology
Aline Matos Arrais, Angelo Jose Burla Dias, Claudio Luiz Melo de Souza, Alinne Gloria Curcio, Marco Roberto Bourg de Mello
Summary: This study evaluated the effect of phospholipase C (PLC) on the capacitation of cryopreserved ovine semen. The results showed that the inhibitor U73122 can prevent premature capacitation and acrosome reaction induced by the freezing procedure, without affecting the kinetics and integrity of the sperm membranes.