Article
Biochemistry & Molecular Biology
Mari Gogniashvili, Yoshihiro Matsuoka, Tengiz Beridze
Summary: The aim of this study was to genetically characterize hexaploid wheat Triticum aestivum L. Two approaches were used - complete sequencing of chloroplast DNA and PCR-based haplotype analysis of specific gene regions. The results revealed genetic differences between samples collected in different regions, with samples grouped into two main categories: those collected in Armenia, and those collected in Europe. This study identified single nucleotide polymorphisms (SNPs) in various regions of the wheat genome, providing insights into the genetic diversity and evolutionary history of hexaploid wheats.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Kari Johnson, Uyen Cao Chu, Geny Anthony, Emily Wu, Ping Che, Todd J. Jones
Summary: Two wheat transformation methods using morphogenic regulator genes, with or without gene excision, have been developed. These methods achieve independent transformation efficiencies of up to 58% and 75%, respectively, and significantly reduce tissue culture duration. The procedures are simplified, less labor-intensive, higher-throughput, and cost-effective.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biotechnology & Applied Microbiology
Gazaldeep Kaur, Vishnu Shukla, Varsha Meena, Anil Kumar, Deepshikha Tyagi, Jagtar Singh, Pramod Kaitheri Kandoth, Shrikant Mantri, Hatem Rouached, Ajay Kumar Pandey
Summary: Iron (Fe) and phosphorus (P) are essential mineral nutrients for plant growth, and their molecular interactions in crops remain unclear. This study analyzed the physiological and molecular responses of hexaploid wheat to individual and combined Fe and P deficiencies, revealing significant gene expression changes and metabolite accumulation, providing insights for developing strategies to enhance crop resilience in limited nutrient conditions.
Article
Plant Sciences
Jianhui Ma, Meng Yuan, Bo Sun, Daijing Zhang, Jie Zhang, Chunxi Li, Yun Shao, Wei Liu, Lina Jiang
Summary: The study identified the evolutionary stages and expansion of NAC transcription factors in green plants, particularly in hexaploid wheat. The results showed that tandem replications during polyploidization play a significant role in increasing the number of NAC transcription factors in wheat. Additionally, the analysis of TaNACs in wheat revealed distinct groups and clusters, indicating the presence of neofunctionalization and subfunctionalization genes which are important in responding to various stresses.
Article
Plant Sciences
Amanda J. Burridge, Mark O. Winfield, Paul A. Wilkinson, Alexandra M. Przewieslik-Allen, Keith J. Edwards, Gary L. A. Barker
Summary: In this study, the bread wheat genome was analyzed using exome capture and sequencing techniques, revealing variable regions and potential undocumented introgressions.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Christina R. Ingvardsen, Julio A. Massange-Sanchez, Finn Borum, Winnie S. Fuchtbauer, Merethe Bagge, Soren Knudsen, Per L. Gregersen
Summary: The introduction of mlo-based resistance in wheat is a significant advancement in crop breeding for durable disease resistance. By screening mutant plants, we obtained triple homozygous mlo mutant lines that showed highly effective resistance against powdery mildew attacks. These mutations all contributed to resistance, but had varying effects on the occurrence of symptoms.
Article
Plant Sciences
Dmitry Miroshnichenko, Vadim Timerbaev, Anna Klementyeva, Alexander Pushin, Tatiana Sidorova, Dmitry Litvinov, Lubov Nazarova, Olga Shulga, Mikhail Divashuk, Gennady Karlov, Elena Salina, Sergey Dolgov
Summary: The VRN-1 gene plays a crucial role in regulating the flowering time of wheat. In this study, the researchers used CRISPR/Cas9 to modify the promoter sequence of the VRN-A1 gene and successfully manipulated the heading time of wheat without affecting its yield characteristics.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Lingli Li, Yingjun Zhang, Yong Zhang, Ming Li, Dengan Xu, Xiuling Tian, Jie Song, Xumei Luo, Lina Xie, Desen Wang, Zhonghu He, Xianchun Xia, Yan Zhang, Shuanghe Cao
Summary: This study identified four QTL associated with PHS resistance in wheat, providing valuable genetic resources and breeding tools. Development of KASP markers and validation of their effectiveness in a test panel of cultivars offer new approaches for marker-assisted selection in wheat breeding.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Firdissa E. Bokore, Richard D. Cuthbert, Ron E. Knox, Colin W. Hiebert, Curtis J. Pozniak, Samia Berraies, Yuefeng Ruan, Brad Meyer, Pierre Hucl, Brent D. McCallum
Summary: This study aimed to identify and map the leaf rust resistance of six Canadian Western Red Spring wheat cultivars. Three doubled haploid populations were evaluated for seedling resistance and adult plant disease response and genotyped with a SNP array. Multiple QTL for leaf rust resistance were found in each population, with contributions from different cultivars. The results provide valuable information for resistance breeding and expanding our knowledge of leaf rust resistance genes in Canadian wheat.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biotechnology & Applied Microbiology
Siyu Zhang, Pei Du, Xueying Lu, Jiaxin Fang, Jiaqi Wang, Xuejun Chen, Jianyong Chen, Hao Wu, Yang Yang, Hisashi Tsujimoto, Chenggen Chu, Zengjun Qi
Summary: This study investigates the impact of chromosome variations on wheat evolution using synthetic hexaploid wheat (SHW). Analysis of SHW lines using chromosome FISH and SNP marker clustering reveals non-random chromosome variations, indicating the occurrence of similar variations in the early stages of wheat polyploidization and their important role in wheat evolution.
Article
Biochemistry & Molecular Biology
Danyu Yao, Waqas Ijaz, Yi Liu, Jinghuang Hu, Wentao Peng, Bowen Zhang, Xiaolan Wen, Juan Wang, Dan Qiu, Hongjie Li, Shihe Xiao, Guozhong Sun
Summary: In this study, the powdery mildew resistance gene pmXMM was localized in Chinese landrace Xiaomaomai using bulked segregant RNA sequencing and comparative genomics analysis. Genetic analysis and SNP markers revealed that pmXMM is located at the distal end of chromosome 2AL. It was confirmed that Xiaomaomai carries a Pm4 allele and pmXMM is identical to Pm4d and Pm4e at the sequence level.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Tianheng Ren, Zixin Sun, Yuling Hu, Zhenglong Ren, Feiquan Tan, Peigao Luo, Zhi Li
Summary: In this study, resistant genes from rye were successfully introduced into wheat through hybridization, which is of great significance for improving wheat disease resistance.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Firdissa E. Bokore, Ron E. Knox, Colin W. Hiebert, Richard D. Cuthbert, Ron M. DePauw, Brad Meyer, Amidou N'Diaye, Curtis J. Pozniak, Brent D. McCallum
Summary: Carberry is a widely grown spring wheat cultivar in Canada with high level of leaf rust resistance. The genetic basis of Carberry's resistance includes multiple genes, such as Lr46 and Lr34. Despite the evolution of virulence in the pathogen population towards other resistance genes, Carberry's resistance remains effective.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Agronomy
Fan Yang, Jingjuan Zhang, Yun Zhao, Qier Liu, Shahidul Islam, Wuyun Yang, Wujun Ma
Summary: The glutamine synthetase gene TaGSr-4B is a potential candidate gene for the QTL of thousand grain weight on chromosome 4B in wheat. The gene marker is ready for wheat breeding.
THEORETICAL AND APPLIED GENETICS
(2022)
Article
Plant Sciences
Kim Osman, Uthman Algopishi, James D. Higgins, Ian R. Henderson, Keith J. Edwards, F. Chris H. Franklin, Eugenio Sanchez-Moran
Summary: Meiotic recombination in wheat exhibits biased distribution of crossovers towards distal regions of chromosomes, creating challenges for plant breeders. Recent advances in wheat genomics and genome engineering, along with well-developed cytogenetics, provide new opportunities for manipulating recombination and unlocking genetic variation in wheat. This study provides a cytogenetic framework for functional studies and ongoing initiatives to manipulate recombination in the wheat genome.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Agronomy
Zibei Lin, Hannah Robinson, Jayfred Godoy, Allan Rattey, David Moody, Daniel Mullan, Gabriel Keeble-Gagnere, Kerrie Forrest, Josquin Tibbits, Matthew J. Hayden, Hans Daetwyler, Dunia Pino Del Carpio
Summary: The study introduced two methods for categorizing trials into clusters on unbalanced data to reduce the variance resulting from genotype x environment interaction (GEI). Testing with empirical barley yield data in Australia showed both methods achieved comparable predictive accuracies, with the cluster method outperforming the non-GEI model. Within-cluster validations demonstrated higher predictive accuracies when training populations were from the same cluster.
Article
Plant Sciences
Vani Kulkarni, Tim Sawbridge, Sukhjiwan Kaur, Matthew Hayden, Anthony T. Slater, Sally L. Norton
Summary: This study established a high throughput hydroponics screening method to identify aluminium toxicity tolerant lentil accessions. Some new sources of aluminium tolerant lentil germplasm were found, showing potential for breeding new varieties with aluminium toxicity tolerance. High performing accessions in acid soil screening could be further evaluated for yield potential under field conditions.
PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS
(2021)
Article
Agronomy
Md. Mahbubur Rahman, Philip Davies, Urmil Bansal, Raj Pasam, Matthew Hayden, Richard Trethowan
Summary: The study evaluated the relationship between resistance and tolerance in wheat genotypes, finding transgressive segregation for both traits. A genome wide association analysis identified 43 QTLs associated with resistance and tolerance traits.
FIELD CROPS RESEARCH
(2021)
Article
Multidisciplinary Sciences
Sameer Joshi, Emily Thoday-Kennedy, Hans D. Daetwyler, Matthew Hayden, German Spangenberg, Surya Kant
Summary: The study utilized digital imaging technology for rapid and precise screening of safflower genotypes for drought tolerance in controlled environments. The results indicate that digital traits can serve as proxy measures for plant growth, aiding in the identification of drought tolerance in a large number of safflower genotypes.
Article
Agronomy
Reem Joukhadar, Rebecca Thistlethwaite, Richard M. Trethowan, Matthew J. Hayden, James Stangoulis, Suong Cu, Hans D. Daetwyler
Summary: Genomic selection was successfully used to predict the concentrations of 13 nutritional elements in wheat grain, showing potential for wheat biofortification breeding. BayesR model demonstrated higher prediction accuracy for mineral concentrations, while BRR model performed well in predicting grain yield.
THEORETICAL AND APPLIED GENETICS
(2021)
Article
Plant Sciences
Emily Thoday-Kennedy, Sameer Joshi, Hans D. Daetwyler, Matthew Hayden, David Hudson, German Spangenberg, Surya Kant
Summary: Salinity is a major factor contributing to land degradation and reduced crop yield, highlighting the need for improving salt tolerance in crop varieties. Safflower, a versatile oilseed crop capable of growing in saline and arid conditions, can be effectively differentiated for salt responses through digital phenotyping. Experimental results suggest that safflower salt tolerance is linked to ion accumulation and biomass, indicating the potential of digital phenotyping in identifying candidate genotypes for salt tolerance.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Delphine Vincent, AnhDuyen Bui, Doris Ram, Vilnis Ezernieks, Frank Bedon, Joe Panozzo, Pankaj Maharjan, Simone Rochfort, Hans Daetwyler, Matthew Hayden
Summary: This study optimized a LC-MS shotgun quantitative proteomics method to screen wheat genotypes for breeding lines with better performance. A large number of wheat proteins were identified using this method, and data mining tools were used to explore the flour proteome.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Sang He, Yong Jiang, Rebecca Thistlethwaite, Matthew J. Hayden, Richard Trethowan, Hans D. Daetwyler
Summary: The study suggests that genomics-based sparse phenotyping can improve selection response in the middle stages of crop breeding programs, with the effectiveness depending on the correlations of phenotypes between different environments.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Gabriel Keeble-Gagnere, Raj Pasam, Kerrie L. Forrest, Debbie Wong, Hannah Robinson, Jayfred Godoy, Allan Rattey, David Moody, Daniel Mullan, Tresslyn Walmsley, Hans D. Daetwyler, Josquin Tibbits, Matthew J. Hayden
Summary: Array-based SNP genotyping platforms have lower error and missing data rates compared to genotyping-by-sequencing technologies. In this study, a novel approach was described for the design of cost-effective imputation-enabled SNP genotyping arrays applicable to any animal or plant species, demonstrated through the development of the Illumina Infinium Wheat Barley 40K SNP array Version 1.0. The new array delivers high quality and resolution data for wheat and barley, aiming to capture haplotypic diversity while minimizing ascertainment bias and improving statistical power for GWAS and genomic selection.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Neelesh Sharma, Bikram Pratap Banerjee, Matthew Hayden, Surya Kant
Summary: Advanced plant phenotyping techniques using different sensors for image acquisition and analysis are helping to deliver improved crop varieties faster.
Article
Biochemistry & Molecular Biology
Xiaoqing Li, Anton P. Wasson, Alexander B. Zwart, Alex Whan, Peter R. Ryan, Kerrie Forrest, Matthew Hayden, Sabrina Chin, Richard Richards, Emmanuel Delhaize
Summary: Root architecture determines the efficiency of nutrient and water absorption in plants. We crossed two wheat cultivars with contrasting root morphologies to generate a recombinant inbred lines population. The plants were grown under controlled conditions and assessed for root and shoot traits. By comparing gene lists, we identified genes associated with root morphology in wheat and determined their physical locations on the Chinese Spring reference genome.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Raj K. Pasam, Surya Kant, Emily Thoday-Kennedy, M. Adam Dimech, Sameer Joshi, Gabriel Keeble-Gagnere, Kerrie Forrest, Josquin Tibbits, Matthew Hayden
Summary: Soil salinity stress leads to significant yield losses in plants. To sustain yields in saline soils, it is important to identify crop varieties tolerant to salinity through genotyping and phenotyping. This study used digital phenotyping to investigate the growth response of a diverse collection of wheat accessions to salinity stress. The results showed that digital plant traits can be used to select salinity-tolerant accessions. A genome-wide association study identified novel genes and QTL for salinity tolerance. This study provides insights into the genetic basis of salinity tolerance and identifies potential candidate genes for future crop breeding.
Article
Agronomy
Emily Thoday-Kennedy, Bikram Banerjee, Joe Panozzo, Pankaj Maharjan, David Hudson, German Spangenberg, Matthew Hayden, Surya Kant
Summary: In this study, field trials were conducted to assess crop traits and potential uses of safflower. Unmanned aerial vehicle (UAV) multispectral data were used to estimate vegetation indices and model crop yield. The results showed that crop traits and performance were influenced by environmental conditions, particularly rainfall. UAV-multispectral derived data proved to be useful for phenotyping safflower and estimating key agronomical traits.
Article
Biochemistry & Molecular Biology
Md Arifuzzaman, Matthias Jost, Meinan Wang, Xianming Chen, Dragan Perovic, Robert F. Park, Matthew Rouse, Kerrie Forrest, Matthew Hayden, Ghazanfar Abbas Khan, Peter M. Dracatos
Summary: In this study, a genotype-by-sequencing approach was used to identify rust resistance alleles in a collection of diverse barley landraces and cultivars. Resistance factors were found on chromosomes 1H, 2H, 4H, and 5H. Additionally, a QTL on chromosome 5HL conferred resistance to both leaf rust and stripe rust.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Huanhuan Zhao, Yongjun Li, Joanna Petkowski, Surya Kant, Matthew J. Hayden, Hans D. Daetwyler
Summary: The study investigated the population structure of a safflower collection sourced from the Australian Grain Genebank and assessed the potential of genomic prediction (GP) to evaluate grain yield and related traits. Genomic best linear unbiased prediction (GBLUP) showed promising prediction accuracies, indicating the feasibility of GP in safflower evaluation and fast introgression of desired traits from genebank germplasm into breeding lines.