Article
Biochemical Research Methods
Mengjie Qie, Shuangyue Li, Chuntao Guo, Shuming Yang, Yan Zhao
Summary: A simple and effective method was developed to determine fifteen toxic alkaloids in forage grass using the QuEChERS method and LC-MS/MS. The method was validated with high accuracy and reproducibility, making it suitable for monitoring and analyzing toxic alkaloids.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Food Science & Technology
Kim Stanford, Karen S. Schwartzkopf-Genswein, Daniela M. Melendez, Skyler Ngo, Michael Harding, Tim A. McAllister, Dian Schatzmayr, Mary Lou Swift, Barry Blakley, Gabriel O. Ribeiro
Summary: Studies evaluated the effects of heating and pelleting on concentrations of ergot alkaloids, followed by a feeding trial. Results showed that heating and pelleting increased alkaloid concentrations, but did not significantly impact growth performance or internal parameters.
Article
Biochemistry & Molecular Biology
Sunyeong Jang, Hyungju Seo, Hojin Kim, Hyoyoung Kim, Jongsung Ahn, Hyunjeong Cho, Sunghie Hong, Seunghwa Lee, Taewoong Na
Summary: A method for the rapid and quantitative analysis of 30 veterinary drugs in feed samples was developed and validated. The method showed good linearity and recovery rates, and can be used for screening residual multiclass veterinary drugs in feed samples.
Article
Food Science & Technology
Veronica Maria Teresa Lattanzio, Emanuela Verdini, Stefano Sdogati, Angela Caporali, Biancamaria Ciasca, Ivan Pecorelli
Summary: This study reports monitoring data of 12 ergot alkaloids in cereal and cereal-derived products collected in Italy from 2017 to 2020. An LC-MS/MS method was used for analysis, with verified analytical performance and validity of generated data. Results showed that most samples contained low levels of ergot alkaloids, but some samples also exhibited high contamination levels.
Article
Environmental Sciences
Ruiguo Wang, Yuan Huang, Shujun Dong, Peilong Wang, Xiaoou Su
Summary: Animal-derived food plays a significant role in human exposure to bisphenol compounds, with limited studies on the source of bisphenols in animal feed. This study examined the occurrence of BPs in animal feed packaging and their migration into feed, confirming that BPA can migrate from plastic packaging into solid feed. Results show that contact time and initial BP concentration affect the migration extent, expanding knowledge on the origin of BPs in the food chain.
Article
Food Science & Technology
Hyoyoung Kim, Eun Joo Baek, Byeung Gon Shin, Ho Jin Kim, Jang-Eok Kim
Summary: A high-performance liquid chromatography tandem mass spectrometry method was developed and successfully applied for the simultaneous determination of multiple mycotoxins in three types of grains. The results of the analysis of Korean grain samples demonstrated the level of mycotoxin contamination.
Article
Toxicology
Jochem Louisse, Patrick P. J. Mulder, Arjen Gerssen, Geert Stoopen, Deborah Rijkers, Milou G. M. van de Schans, Ad A. C. M. Peijnenburg
Summary: “The study utilized a bioassay-directed analysis approach to identify potentially toxic PAs not yet included in monitoring programs. The findings suggest that this method is effective in identifying candidate PAs with genotoxic activity.”
ARCHIVES OF TOXICOLOGY
(2022)
Article
Chemistry, Physical
Shahida Anusha Siddiqui, Nur Alim Bahmid, Ahmed Taha, Abdel-Moneim Eid Abdel-Moneim, Abdelrazeq M. Shehata, Chen Tan, Mohammad Saeed Kharazmi, Yuan Li, Elham Assadpour, Roberto Castro-Munoz, Seid Mahdi Jafari
Summary: This study reviews the application of nanoencapsulation in animal feedings and relevant drugs. Nanoencapsulation design can protect feed bioactives and improve their absorption and release in animals. Chitosan is found to be the most effective carrier. Nanoparticles and nanocapsules are commonly used nanocarriers. Nanofibers, nanohydrogels, and nanoemulsions have not been applied in feeds yet. For ruminants, nano-minerals, vitamins, phytochemicals, essential fatty acids, and drugs are encapsulated using nanoparticles for improved delivery and bioavailability. Safety and regulatory issues, as well as advantages and disadvantages of nanoencapsulation in animal feeds, are also discussed.
ADVANCES IN COLLOID AND INTERFACE SCIENCE
(2022)
Article
Chemistry, Applied
Rafaelle Pereira Cordeiro, Patricia Aparecida de Campos Braga, Maria Juliete Souza Rocha, Edsandra Campos Chagas, Felix Guillermo Reyes Reyes
Summary: This study investigated the residue depletion of albendazole and its metabolites in tambaqui parasitized by acanthocephalan. The results showed that ABZ was rapidly metabolized in the target tissue, with ABZSO being the most persistent metabolite. It is suggested that the total residue be considered as a marker residue for fish in legislative frameworks.
FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT
(2021)
Review
Chemistry, Applied
Stephen W. C. Chung
Summary: Analysis of EAs in cereals and feed has been a focus for decades, with liquid chromatography with fluorescence detection and LC-MS now being the main analytical methods. However, matrix effects in LC-MS analysis can lead to underestimation or overestimation of EAs levels, especially for certain epimers.
FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT
(2021)
Review
Food Science & Technology
Bo Wang, Kaizhou Xie, Kiho Lee
Summary: The review highlights the progress made in the detection of veterinary drug residues over the past decade, emphasizing the potential harm of veterinary drugs to human health and the environment. Future research should focus on the development of extraction methods and detection technologies, as well as exploring more advanced methods such as the application of biosensors.
Article
Veterinary Sciences
Katie Waine, Constance White, Rachel S. Dean, Chris Hudson, Jonathan N. Huxley, Marnie L. Brennan
Summary: This study explores the process and feasibility of prospective and retrospective data collection for farm animal clinical audit at three different practices in the UK. It found that retrospective clinical audit was challenging due to barriers in establishing diagnosis and patient identity, while prospective data collection was feasible with sufficient case accrual and practitioner engagement. Further research is needed to investigate the use of clinical coding and alternatives to practice management software to improve retrospective data availability for clinical audit.
VETERINARY SCIENCES
(2021)
Article
Food Science & Technology
Mikael Pedersen, Elena Hakme, Ederina Ninga, Henrik L. Frandsen
Summary: This study developed a LC-HRMS method for screening, quantification, and identification of veterinary drugs and pesticide residues in pig muscle. The method involved extraction of tissues with acidic methanol/water with EDTA, followed by solid phase extraction. The extracts were analyzed using a MaXis Q-TOF mass spectrometer, with criteria established for screening and identification.
Article
Environmental Sciences
Felipe Penagos-Tabares, Michael Sulyok, Johannes Faas, Rudolf Krska, Ratchaneewan Khiaosa-ard, Qendrim Zebeli
Summary: Modern agriculture heavily relies on the use of pesticides and veterinary drugs, making it crucial to control the exposure to these substances in the feed and food chain. This study investigated the residues of a wide range of pesticides and veterinary drugs in dairy cattle diets. Samples collected from three Austrian provinces revealed the presence of 16 residues, including pesticides such as fluopyram, piperonyl butoxide, and diethyltoluamide. The study also identified three residues of veterinary drugs. The findings highlight the occurrence of pesticide cocktails at low doses in the feed/food chain, emphasizing the need for further research on the potential synergistic effects of co-occurring chemicals.
ENVIRONMENTAL POLLUTION
(2023)
Article
Biochemical Research Methods
Garnet McRae, Donald M. Leek, Juris Meija, Bryn Shurmer, Steven J. Lehotay, Joachim Polzer, Jeremy E. Melanson, Zoltan Mester
Summary: A freeze-dried bovine muscle-certified reference material (CRM), called BOTS-1, containing residues of commonly used veterinary drugs, was produced and certified. Liquid chromatography tandem mass spectrometry (LC-MS/MS) methods were used for value assignment, along with isotope dilution and standard addition approaches. Data from multiple organizations were used for value assignment. Results were obtained through an international inter-laboratory comparison. Primary standards were characterized using quantitative NMR (1H-qNMR). The certified mass fractions of the veterinary drug residues were reported with expanded uncertainties.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Applied
Silvia Marin, Olga Pardo, Rosario Baguena, Guillermina Font, Vicent Yusa
FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT
(2017)
Article
Environmental Sciences
Clara Coscolla, Antonio Lopez, Abderrazak Yahyaoui, Patrice Colin, Corine Robin, Quentin Poinsignon, Vicent Yusa
SCIENCE OF THE TOTAL ENVIRONMENT
(2017)
Article
Chemistry, Analytical
Eva Soriano, Vicent Yusa, Agustin Pastor, Miguel de la Guardia
MICROCHEMICAL JOURNAL
(2018)
Article
Environmental Sciences
Vicent Yusa, Rosa Perez, Alfredo Sanchez, Olga Pardo, Marta Roca
SCIENCE OF THE TOTAL ENVIRONMENT
(2018)
Article
Chemistry, Analytical
Maria Isabel Beser, Olga Pardo, Joaquim Beltran, Vicent Yusa
ANALYTICA CHIMICA ACTA
(2019)
Article
Environmental Sciences
Rosa Perez, Trinidad Suelves, Yolanda Molina, Francisca Corpas-Burgos, Vicent Yusa
Article
Chemistry, Analytical
Pablo Dualde, Clara Coscolla, Agustin Pastor, Vicent Yusa
INTERNATIONAL JOURNAL OF ANALYTICAL CHEMISTRY
(2019)
Article
Environmental Sciences
Pablo Dualde, Olga Pardo, Francisca Corpas-Burgos, Julia Kuligowski, Maria Gormaz, Maximo Vento, Agustin Pastor, Vicent Yusa
SCIENCE OF THE TOTAL ENVIRONMENT
(2019)
Article
Environmental Sciences
Pablo Dualde, Olga Pardo, Francisca Corpas-Burgos, Julia Kuligowski, Maria Gormaz, Maximo Vento, Agustin Pastor, Vicent Yusa
Article
Environmental Sciences
Clara Coscolla, Alfredo Sanchez, Francisca Corpas-Burgos, Antonio Lopez, Rosa Perez, Julia Kuligowski, Maximo Vento, Vicent Yusa
Summary: The study analyzed the exposure and risk assessment of toxic and essential elements in Spanish women of reproductive age, finding high concentrations of arsenic positively associated with fish intake, and molybdenum with cereal and pastry intake. Some mothers showed lower levels of selenium and molybdenum intake, indicating inadequate nutritional intake.
ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY
(2021)
Article
Environmental Sciences
Sandra F. Fernandez, Olga Pardo, Francisca Corpas-Burgos, Vicent Yusa
SCIENCE OF THE TOTAL ENVIRONMENT
(2020)
Article
Chemistry, Analytical
Vicent Yusa, Antonio Lopez, Pablo Dualde, Olga Pardo, Igor Fochi, Pablo Miralles, Clara Coscolla
Summary: A new, fast, and automated analytical strategy has tentatively identified twenty-four substances in food contact polycarbonate, most of which are plasticizers, slip agents, antioxidants, ultraviolet stabilizers, and fragrances. The workflow includes intelligent data acquisition using LC-Orbitrap Tribrid-HRMS (MS3) and automated data processing using Compound Discoverer TM. A strict criterion has been established for high confidence identification, and a risk assessment was conducted using the toxicological concern threshold approach. Except for one compound, the identified substances do not pose a risk in migration from the food contact polycarbonate.
INTERNATIONAL JOURNAL OF ANALYTICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Pablo Miralles, Vicent Yusa, Yovana Sanchis, Clara Coscolla
Summary: A GC-HRMS analytical method has been developed and validated for the determination of 60 migrant substances in plastic food contact materials, showing satisfactory linearity, precision, and relative recovery. The method was successfully applied to field samples, demonstrating compliance with current European regulations.
Article
Environmental Sciences
Carmen Saez, Alfredo Sanchez, Vicent Yusa, Pablo Dualde, Sandra F. Fernandez, Antonio Lopez, Francisca Corpas-Burgos, Miguel Angel Aguirre, Clara Coscolla
Summary: This study investigated the exposure of 119 Spanish women of reproductive age to essential and toxic elements, finding differences in toxic element levels between urban and rural areas. Diet preferences were also found to influence exposure levels, with higher levels of certain elements detected in women with specific dietary habits. Overall, health risks due to exposure to the studied toxic elements were expected to be low for the female population under study.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2021)
Article
Environmental Sciences
Pablo Miralles, Vicent Yusa, Adriana Pineda, Clara Coscolla
Summary: A fast and automated approach using GC-Q-Orbitrap HRMS was developed for the tentative identification and risk assessment of unknown substances in plastic food contact materials. The study applied strict identification criteria and a threshold of toxicological concern (TTC) approach for risk assessment, showing that there was no risk associated with the release of the identified substances. Additional studies on toxicity of unidentified substances and potential mixture toxicity should be conducted.
Article
Chemistry, Analytical
Qian Wu, Li Tian, Xiangyu Shan, Huiling Li, Chao Li, Juan Lu
Summary: A novel ECL sensor was developed by modifying Fe3CuO4-Ru(bpy)3+ and GO-CdS@ZnS on the electrode for the detection of permethrin. The sensor exhibited high sensitivity and a wide detection range.
Article
Chemistry, Analytical
Tingting Gong, Lei Liao, Bingying Jiang, Ruo Yuan, Yun Xiang
Summary: In this study, a fluorescent biosensing system for high sensitivity detection of MT was established using a new Ag+-stabilized DNA triplex probe (Ag+-SDTP) combined with catalytic hairpin assembly (CHA) and CRISPR/Cas12a signal enhancements. The sensing method is able to selectively detect MT from other non-specific molecules and can achieve low level detection of MT in diluted human serums, demonstrating its potential for monitoring disease-specific MT biomarker at trace levels.
Article
Chemistry, Analytical
Estrella Sanz Rodriguez, Kyra Wood, Paul R. Haddad, Brett Paull
Summary: This study presents a simple, rapid, and reliable method for determining boron in aqueous solutions using suppressed ion chromatography coupled to electrospray ionisation-triple quadrupole mass spectrometry. The method achieved a low limit of detection and demonstrated excellent analytical performance.
Article
Chemistry, Analytical
Aline Cournut, Paul Moustiez, Yannick Coffinier, Christine Enjalbal, Claudia Bich
Summary: Alzheimer's disease is a major cause of senile dementia and is characterized by the aggregation of neurofibrillary tangles and amyloid plaques in the brain. The A beta 1-42 peptide is considered the primary cause of neurotoxicity due to its insolubility and subsequent aggregation. A beta 1-42 and A beta 1-40 have been identified as relevant biomarkers for AD diagnosis, and SurfaceAssisted Laser Desorption/Ionization Mass Spectrometry (SALDI-MS) was used to monitor the A beta 1-42/A beta 1-40 ratio without prior sample treatment.
Article
Chemistry, Analytical
Dotse Selali Chormey, Buse Tugba Zaman, Tulay Borahan Kustanto, Sezin Erarpat Bodur, Suleyman Bodur, Elif Ozturk Er, Sezgin Bakirdere
Summary: This review provides an overview of the harmful effects of endocrine disrupting chemicals (EDCs) on human health and introduces the methods for analyzing EDCs. Recent research has focused on using green chemicals, such as deep eutectic solvents (DESs), for microextraction to ensure environmental safety.
Article
Chemistry, Analytical
Yan Zhang, Renfeng Xu, Jingjing Wu, Zhenghong Zhang, Yuhuang Wang, Hongqin Yang, Sheng Zhang
Summary: This study visualized the pore-like structures in prostate cancer cell membranes after treatment with the anticancer drug paclitaxel, demonstrating its mechanism of action related to oxidative damage.
Article
Chemistry, Analytical
Chihong Ma, Qin Zhou, Jinjin Shi, Hua Gao, Di Huang, Huimin Xue, Han Wang, Zhenzhong Zhang, Sen Yang, Junli Zhang, Kaixiang Zhang
Summary: We developed a CRISPR-empowered electrochemical biosensor, named PER-E-CRISPR, for amplification-free and sensitive detection of miR-21. The biosensor combines the advantages of CRISPR/Cas13a and primer exchange reaction (PER) for target amplification. Under optimized conditions, the PER-E-CRISPR assay exhibits a linear range from 10-13 to 10-7 M and a limit of detection of 30.2 fM. The biosensor shows excellent performance in actual plasma, indicating promising prospects for miRNA detection in molecular diagnosis.
Article
Chemistry, Analytical
Gang Liang, Jie Zhao, Yufei Gao, Tao Xie, Jianhui Zhen, Ligang Pan, Wenwen Gong
Summary: Molecular docking (MD) analysis is widely used for studying the interaction between aptamers and small molecules. This study established steady-state aptamers of tertiary structures (SATS) using specific aptamers of tetracycline antibiotics as docking models. Molecular docking results revealed multiple binding sites in the SATS of aptamers, with significant variations in binding free energy (BFE) and docking score (DS). The study also proposed a method for MD analysis based on SATS, providing insights into binding mode and predicting binding sites.
Article
Chemistry, Analytical
Yixuan Xu, Changchang Teng, Huiping Dang, Dalong Yin, Lifeng Yan
Summary: Stable organic radical molecules with high NIR fluorescence quantum efficiency have been synthesized and encapsulated into nanoparticles, which show great potential for both photodynamic therapy (PDT) and photothermal therapy (PTT) of tumors.
Article
Chemistry, Analytical
Yu Su, Dandan Yang, Yanjie Wang, Jie Ding, Lan Ding, Daqian Song
Summary: In this study, a highly selective molecularly imprinted polymer Cu-MIPs@CS was designed and synthesized for bisphenol A (BPA). Cu-MIPs@CS showed significantly enhanced imprinting factor and selective factor towards BPA compared to MIPs@CS. It also exhibited superior discrimination ability between BPA and its structural analogue. Additionally, Cu-MIPs@CS were successfully applied as a solid phase extraction adsorbent for the determination of BPA in drinking water samples.
Article
Chemistry, Analytical
Yi Qiao, Qiongdan Zhang, Yukun He, Tianguang Cheng, Jing Tu
Summary: Single cell heterogeneity plays an important role in many biological phenomena, and distinguishing cells with specific mutations is beneficial for clinical diagnosis and drug screening. This study introduces a gel plate platform that can distinguish cell types based on their phenotypes, with low equipment requirement. The gel platform has potential in point-of-care circumstances and single-cell stimulation responses that prioritize efficiency and simplicity.
Article
Chemistry, Analytical
Chengke Wang, Shuyang Sun, Ping Wang, Huawei Zhao, Wenling Li
Summary: The food safety of aquatic products is a global concern. Nanotechnology-based analyses have advantages in the detection of bacteria, metal ions, and small molecule contaminants. This review summarizes the recent advances in biosensing strategies for aquatic products and highlights the application of nanomaterials, lateral flow-based biosensors, surface-enhanced Raman scattering, microfluidic chips, and molecular imprinting technologies.
Article
Chemistry, Analytical
Liping Zhu, Zeng Tang, Xuemei Zhang, Li Zhu, Tian Meng, Linying Yu, Ting Xiao, Shasha Lu, Xiaoli Xiong, Xiurong Yang
Summary: A sensitive off-on electrochemiluminescence DNA sensor was developed using Exo III-assisted cascade amplification system. The sensor achieved high selectivity and ultra-high sensitivity in detecting target DNA by utilizing ECL and SPR effects. This study provides a new perspective for designing highly sensitive and programmable ECL biosensors.
Article
Chemistry, Analytical
Tong Li, Mengxu Sun, Suping Xia, Ting Huang, Rong-Tian Li, Chunrong Li, Zong Dai, Jin-Xiang Chen, Jun Chen, Nuan Jia
Summary: Here, we developed a binary system based on DNA tetrahedron and fluorogenic RNA aptamers for highly specific and label-free mRNA imaging in living cells. This system achieved high specificity label-free detection of nucleic acids with a detection limit of 1.34 nM. The method has potential applications in early disease diagnosis and new drug development.
Article
Chemistry, Analytical
Yao Zhao, Shu Zhang, Wang Yao, Yuxuan Zhu, Jing Qian, Juan Yang, Nianjun Yang
Summary: A new hierarchical nanostructured composite with high conductivity and catalytic activity is synthesized. The sensor exhibits significant catalytic effect and high sensitivity for monitoring the environmental endocrine disruptor bisphenol A (BPA).