Review
Biochemistry & Molecular Biology
Yong-Jin Kim, Amin Tamadon, Yoon-Young Kim, Byeong-Cheol Kang, Seung-Yup Ku
Summary: This review focuses on the current knowledge of epigenetic regulation of cardiomyocyte proliferation and differentiation from embryonic and induced pluripotent stem cells through histone modification and microRNAs, the maintenance of pluripotency, and its alteration during cardiac lineage differentiation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Karlijn A. L. Hasaart, Freek Manders, Joske Ubels, Mark Verheul, Markus J. van Roosmalen, Niels M. Groenen, Rurika Oka, Ewart Kuijk, Susana M. Chuva de Sousa Lopes, Ruben van Boxtel
Summary: This study compares the mutation burden between cultured iPSCs and their isogenic embryonic cells, finding that iPSCs maintain genomic integrity during culture at a similar degree as pluripotent cells in vivo.
Article
Chemistry, Multidisciplinary
Jonathan Sai-Hong Chui, Teresa Izuel-Idoype, Alessandra Qualizza, Rita Pires de Almeida, Lindsey Piessens, Bernard K. van der Veer, Gert Vanmarcke, Aneta Malesa, Paraskevi Athanasouli, Ruben Boon, Joris Vriens, Leo van Grunsven, Kian Peng Koh, Catherine M. Verfaillie, Frederic Lluis
Summary: This study demonstrates that a hyperosmolar environment can induce cell cycle arrest and cellular maturation through the regulation of NF-kappa B and WNT signaling. It provides a practical method for differentiating stem cells and suggests the use of higher osmolarity in in vitro cultures to mimic natural conditions.
Article
Biology
Lucie Zilova, Venera Weinhardt, Tinatini Tavhelidse, Christina Schlagheck, Thomas Thumberger, Joachim Wittbrodt
Summary: Organoids derived from rapidly developing teleosts show efficient assembly of anterior neural structures, particularly the retina, within just 4 days. The number of aggregated cells and genetic factors crucially impact morphological changes, reflecting the in vivo situation. High efficiency and rapid development of fish-derived organoids combined with advanced genome editing techniques allow for immediate research on development, disease, and the impact of physical environment on morphogenesis and differentiation.
Article
Biochemical Research Methods
Julia Vallverdu, Raquel A. Martinez Garcia de la Torre, Inge Mannaerts, Stefaan Verhulst, Ayla Smout, Mar Coll, Silvia Arino, Teresa Rubio-Tomas, Beatriz Aguilar-Bravo, Celia Martinez-Sanchez, Delia Blaya, Catherine M. Verfaillie, Leo A. van Grunsven, Pau Sancho-Bru
Summary: Human iPSCs are differentiated into HSCs with growth factors for in vitro modeling. The protocol yields iPSC-HSCs with phenotypic and functional characteristics of primary HSCs, suitable for high-throughput in vitro studies. Coculturing iPSC-HSCs with hepatocytes allows for the formation of 3D hepatic spheroids, enabling modeling and drug screening studies.
Review
Biochemistry & Molecular Biology
Menno ter Huurne, Hendrik G. Stunnenberg
Summary: During early embryonic development, the rapid increase in cell number and the expression of genes that control developmental decisions are tightly regulated and interconnected processes. Transcription factors and signaling pathways play a crucial role in maintaining pluripotency and cell cycle progression, while canonical cell cycle regulators impact molecular networks controlling pluripotency and lineage specification.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Julian O. Kimura, Marcela Bolanos, Lorenzo Ricci, Mansi Srivastava
Summary: This study reveals the cellular mechanism and molecular trajectory for the formation of adult pluripotent stem cells (aPSCs) during embryonic development. By utilizing lineage tracing and single-cell transcriptome profiling, the researchers identified a molecular trajectory for neoblast formation and showed that aPSCs derive from one lineage instead of multiple tissue-specific lineages.
Article
Biochemistry & Molecular Biology
Edward J. Calabrese
Summary: This paper provides a thorough assessment of hormetic dose responses in embryonic stem cells, suggesting that this response may serve as a fundamental and highly conserved evolutionary strategy.
CHEMICO-BIOLOGICAL INTERACTIONS
(2022)
Review
Biochemistry & Molecular Biology
Yahong Wu, Weiwei Zhang
Summary: Pluripotent stem cells, including ESCs and iPSCs, are crucial for studying early development and treating diseases, with ubiquitination playing a key role in pluripotency regulation, particularly through the actions of E3 ligases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Developmental Biology
Andrea L. Bredemeyer, Junedh M. Amrute, Andrew L. Koenig, Rachel A. Idol, Li He, Stephanie A. Luff, Carissa Dege, Jamison M. Leid, Joel D. Schilling, J. Travis Hinson, Mary C. Dinauer, Christopher M. Sturgeon, Kory J. Lavine
Summary: Tissue-resident macrophages play a crucial role in organ homeostasis, tissue repair, remodeling, and regeneration. Researchers have developed a system to generate macrophages that resemble tissue-resident or monocyte-derived subsets, allowing for the study of their function and potential applications in tissue engineering and regenerative medicine.
Review
Cell & Tissue Engineering
Sonam Raj, Sushil K. Jaiswal, Melvin L. DePamphilis
Summary: This review article addresses the enigmatic role of the p53 transcription factor and tumor suppressor in programmed cell death during embryonic development. Contradictory results regarding the dependence of pluripotent embryonic stem cells on p53 for cell death are reconciled based on experimental conditions. The review concludes that p53 is not required for mouse embryonic development, but can accelerate programmed cell death in certain conditions. Additionally, DNA damage in differentiated cells induces p53-dependent cell cycle arrest and senescence.
Article
Biotechnology & Applied Microbiology
Yanyan Chen, Pufeng Huang, Mengda Niu, Chuanhuizi Tian, Tingting Zhang, Zhiping Peng
Summary: This study developed an efficient culture method to generate functional CD8(+) T cells from human induced pluripotent stem cells (hiPSC) and obtained hiPS-CAR-T cells with high antigen-specific cytotoxicity that are not significantly different from CAR T cells found in peripheral blood. This finding may pave the way for the clinical use of hiPSC for cell-based cancer therapy.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Review
Cell Biology
Muhammad Shahid Javaid, Tracie Tan, Naomi Dvir, Alison Anderson, Terence J. O'Brien, Patrick Kwan, Ana Antonic-Baker
Summary: This review reports the current information and progress in using induced pluripotent stem cells to generate patient-specific models of epilepsy, which can be used to study the pathological mechanisms of epilepsy and drug development.
Article
Cell & Tissue Engineering
Kenji Kitajima, Minako Shingai, Hikaru Ando, Mako Hamasaki, Takahiko Hara
Summary: Researchers developed a novel method for hematopoietic differentiation of human-induced pluripotent stem cells to address the issue of low production of CD38(+) hematopoietic progenitor cells. They found that the lack of FLT3 expression was responsible for this problem and solved it by adding interferon-gamma.
Article
Engineering, Biomedical
Hong Cao, Qian Zhou, Chungeng Liu, Yecen Zhang, Minghui Xie, Weihua Qiao, Nianguo Dong
Summary: This study investigates the effect of substrate stiffness on the differentiation of human-induced pluripotent stem cells (h-iPSCs) into valve endothelial-like cells (h-iVECs). The results show that moderate substrate stiffness can enhance the differentiation efficiency of h-iVECs and is regulated by the WNT/Calcineurin signaling pathway. This study emphasizes the importance of using substrate stiffness to achieve a more specific and mature differentiation of h-iVECs.
ACTA BIOMATERIALIA
(2022)
Review
Biochemistry & Molecular Biology
Thomas Colunga, Stephen Dalton
TRENDS IN MOLECULAR MEDICINE
(2018)
Article
Cell Biology
Thomas Colunga, Miranda Hayworth, Sebastian Kress, David M. Reynolds, Luoman Chen, Kristopher L. Nazor, Johannes Baur, Amar M. Singh, Jeanne F. Loring, Marco Metzger, Stephen Dalton
Article
Cell & Tissue Engineering
Jarmon G. Lees, Timothy S. Cliff, Amanda Gammilonghi, James G. Ryall, Stephen Dalton, David K. Gardner, Alexandra J. Harvey
STEM CELLS INTERNATIONAL
(2019)
Article
Cell & Tissue Engineering
Vishnu Dileep, Korey A. Wilson, Claire Marchal, Xiaowen Lyu, Peiyao A. Zhao, Ben Li, Axel Poulet, Daniel A. Bartlett, Juan Carlos Rivera-Mulia, Zhaohui S. Qin, Allan J. Robins, Thomas C. Schulz, Michael J. Kulik, Rachel Patton McCord, Job Dekker, Stephen Dalton, Victor G. Corces, David M. Gilbert
Review
Cell Biology
Siwanon Jirawatnotai, Stephen Dalton, Methichit Wattanapanitch
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2020)
Article
Multidisciplinary Sciences
Amar M. Singh, Liang Zhang, John Avery, Amelia Yin, Yuhong Du, Hui Wang, Zibo Li, Haian Fu, Hang Yin, Stephen Dalton
NATURE COMMUNICATIONS
(2020)
Article
Cell & Tissue Engineering
Liang Zhang, John Avery, Amelia Yin, Amar M. Singh, Timothy S. Cliff, Hang Yin, Stephen Dalton
Article
Multidisciplinary Sciences
Fabian Grubert, Rohith Srivas, Damek Spacek, Maya Kasowski, Mariana Ruiz-Velasco, Nasa Sinnott-Armstrong, Peyton Greenside, Anil Narasimha, Qing Liu, Benjamin Geller, Akshay Sanghi, Michael Kulik, Silin Sa, Marlene Rabinovitch, Anshul Kundaje, Stephen Dalton, Judith B. Zaugg, Michael Snyder
Article
Multidisciplinary Sciences
Tianfang Wang, Sang-Ho Kwon, Xiao Peng, Severine Urdy, Zefu Lu, Robert J. Schmitz, Stephen Dalton, Keith E. Mostov, Shaying Zhao
Article
Genetics & Heredity
Bobby G. Ng, Paulina Sosicka, Francois Fenaille, Annie Harroche, Sandrine Vuillaumier-Barrot, Mindy Porterfield, Zhi-Jie Xia, Shannon Wagner, Michael J. Bamshad, Marie-Christine Vergnes-Boiteux, Sophie Cholet, Stephen Dalton, Anne Dell, Thierry Dupre, Mathieu Fiore, Stuart M. Haslam, Yohann Huguenin, Tadahiro Kumagai, Michael Kulik, Katherine McGoogan, Caroline Michot, Deborah A. Nickerson, Tiffany Pascreau, Delphine Borgel, Kimiyo Raymond, Deepti Warad, Heather Flanagan-Steet, Richard Steet, Michael Tiemeyer, Nathalie Seta, Arnaud Bruneel, Hudson H. Freeze
Summary: The study identified a recurrent mutation in SLC37A4 causing a dominantly inherited congenital disorder of glycosylation characterized by coagulopathy and liver dysfunction with abnormal serum N-glycans. Liver-specific abnormalities in glycosylation were replicated in a CRISPR base-edited hepatoma cell line carrying the mutation. The mutant protein showed relocation to a non-Golgi compartment and altered Golgi morphology and reduced intraluminal pH, potentially explaining the glycosylation alterations.
AMERICAN JOURNAL OF HUMAN GENETICS
(2021)
Article
Multidisciplinary Sciences
Kyle N. Klein, Peiyao A. Zhao, Xiaowen Lyu, Takayo Sasaki, Daniel A. Bartlett, Amar M. Singh, Ipek Tasan, Meng Zhang, Lotte P. Watts, Shin-ichiro Hiraga, Toyoaki Natsume, Xuemeng Zhou, Timour Baslan, Danny Leung, Masato T. Kanemaki, Anne D. Donaldson, Huimin Zhao, Stephen Dalton, Victor G. Corces, David M. Gilbert
Summary: Loss of RIF1 disrupts the temporal order of DNA replication, leading to changes in chromatin modifications and genome architecture. Depletion of RIF1 causes increased heterogeneity between individual cells, resulting in replication-dependent disruption of histone modifications and alterations in genome architecture. These effects are magnified with successive cycles of altered replication timing.
Article
Biochemistry & Molecular Biology
Udom Lao-On, Timothy S. Cliff, Stephen Dalton, Sarawut Jitrapakdee
Summary: This study reveals that the ablation of the PC gene in brown adipocytes results in a 35% reduction in ATP-linked respiration, but does not affect thermogenesis, highlighting the crucial role of PC in supporting fuel oxidation rather than heat production in human brown adipocytes.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Meeting Abstract
Biochemistry & Molecular Biology
Michelle T. Dookwah, Kazuhiro Aoki, Michael J. Kulik, Heidi Ulrichs, Stephen Dalton, Nadja Zeltner, Huiya Yang, Guangping Gao, Kevin A. Strauss, Richard Steet, Michael Tiemeyer
Meeting Abstract
Biochemistry & Molecular Biology
F. Grubert, R. Srivas, D. Spacek, M. Kasowski, M. Ruiz-Velasco, N. Sinnott-Armstrong, P. Greenside, A. Narasimha, Q. Liu, B. Geller, A. Sanghi, M. Kulik, S. Sa, M. Rabinovitch, A. Kundaje, S. Dalton, J. Zaugg, M. Snyder
EUROPEAN JOURNAL OF HUMAN GENETICS
(2020)
Meeting Abstract
Biochemistry & Molecular Biology
Alison V. Nairn, Harrison Grace, Katelyn Rosenbalm, Melina Galizzi, Mitche dela Rosa, Mindy Porterfield, Michael Kulik, J. Michael Pierce, Stephen Dalton, Michael Tiemeyer, Kelley W. Moremen