Article
Cell & Tissue Engineering
Belen Alvarez-Palomo, Anna Veiga, Angel Raya, Margarita Codinach, Silvia Torrents, Laura Ponce Verdugo, Clara Rodriguez-Aierbe, Leopoldo Cuellar, Raquel Alenda, Cristina Arbona, Dolores Hernandez-Maraver, Cristina Fuste, Sergi Querol
Summary: This article describes the process of generating a CD34+ cell product as a starting material to produce HLA-homozygous iPSC following a cost-effective and clinical grade-compliant procedure, starting from unit selection and donor reconsenting. These CD34+ cells serve as the basis for the Spanish bank of haplolines, aimed to provide cell products for clinical research and therapy.
STEM CELL RESEARCH & THERAPY
(2022)
Review
Biochemistry & Molecular Biology
Michaela Oeller, Sandra Laner-Plamberger, Linda Krisch, Eva Rohde, Dirk Strunk, Katharina Schallmoser
Summary: HPL serves as a valuable alternative to fetal bovine serum in cell culture, allowing for efficient and animal serum-free cell propagation. Providers must adhere to strict quality control and regulatory requirements to ensure safe and GMP-compliant production of HPL. The starting material for HPL preparation is fresh or expired platelet concentrates from healthy donors, and the process may involve pathogen reduction technologies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Oncology
P. K. J. D. de Jonge, P. M. M. van Hauten, L. D. Janssen, A. L. de Goede, M. M. Berrien-Elliott, J. M. R. van der Meer, C. M. Mousset, M. W. H. Roeven, M. Foster, N. Blijlevens, W. Hobo, T. A. Fehniger, J. H. Jansen, N. P. M. Schaap, H. Dolstra
Summary: Allogeneic natural killer (NK) cell-based immunotherapy is a promising approach for acute myeloid leukemia (AML). A good manufacturing practice (GMP)-compliant, cytokine-based ex vivo manufacturing process for generating NK cells from CD34(+) hematopoietic stem and progenitor cells (HSPC) has been developed. This process generates a consistent and active NK cell product that meets the requirements for NK cell immunotherapy. The manufactured NK cell batches are used in a clinical trial approved by the Dutch Ethics Committee.
CANCER IMMUNOLOGY IMMUNOTHERAPY
(2023)
Article
Biochemistry & Molecular Biology
Tereza Souralova, Daniela Rehakova, Michal Jeseta, Lenka Tesarova, Jindrich Beranek, Pavel Ventruba, Ales Hampl, Irena Koutna
Summary: Human embryonic stem cells (hESCs) are being used more and more in clinical trials due to their potential to improve the outcomes of treatment for various human diseases. Ensuring the quality of hESCs is crucial, and current good manufacturing practice (cGMP) guidelines are followed during the transport of embryos and the derivation of hESC cultures. This paper presents the derivation of three clinical-grade hESC lines that meet these criteria and demonstrates the development and validation of a system for manufacturing high-quality starting materials suitable for cell therapy according to cGMP.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell & Tissue Engineering
B. Kuebler, B. Alvarez-Palomo, B. Aran, J. Castano, L. Rodriguez, A. Raya, S. Querol Giner, A. Veiga
Summary: This study established the first clinical-grade iPSC haplobank in Spain, utilizing allogenic HLA-matched donors to generate iPSC lines. The iPSCs were expanded and stored under GMP conditions. These iPSCs will be used for the development of advanced therapy medicinal products.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Biochemistry & Molecular Biology
Katia Mareschi, Elena Marini, Alessia Giovanna Santa Banche Niclot, Marta Barone, Giuseppe Pinnetta, Aloe Adamini, Manuela Spadea, Luciana Labanca, Graziella Lucania, Ivana Ferrero, Franca Fagioli
Summary: This study demonstrates that the use of HPL-S is an effective alternative for MSC production under GMP conditions. The protein and growth factor content in HPL-S is comparable to that of HPL-E. Furthermore, MSCs derived from HPL-S show lower HLA-DR expression in immunophenotypic analysis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell & Tissue Engineering
Kei Takada, Ryoko Nakatani, Emiko Moribe, Shizuka Yamazaki-Fujigaki, Mai Fujii, Masayo Furuta, Hirofumi Suemori, Eihachiro Kawase
Summary: This article introduces the establishment of clinical-grade human embryonic stem cell lines KthES11 in Japan according to good manufacturing practice standards. By using xeno-free chemically defined medium and optimizing the protocol, they successfully established five hESC lines, which are of great significance for human pluripotent stem cell therapy applications.
REGENERATIVE THERAPY
(2022)
Article
Computer Science, Artificial Intelligence
Alberto Loffredo, Marvin Carl May, Andrea Matta, Gisela Lanza
Summary: The importance of sustainability in industry is increasing, and energy-efficient control (EEC) is an effective method for improving energy efficiency in production systems. This study develops a novel RL-based model for applying EEC to multi-stage production lines with parallel machine workstations, and the experiments confirm the effectiveness and generality of the approach.
JOURNAL OF INTELLIGENT MANUFACTURING
(2023)
Article
Robotics
Chen Li, Qing Chang, Guoxian Xiao, Jorge Arinez
Summary: This letter presents an integrated data-enabled model that combines system-level and process-level models and analysis. By utilizing sensor data, the system yield can be accurately evaluated, and the root cause of permanent production loss can be identified through the proposed model.
IEEE ROBOTICS AND AUTOMATION LETTERS
(2022)
Review
Immunology
MingYue Kong, Di Zhou
Summary: Human embryonic stem cells have great potential in regenerative medicine, but HLA incompatibility is a major obstacle that requires immunosuppressive therapy. Using gene-editing technology to establish HLA-compatible human embryonic stem cell lines can provide an unlimited source for future transplantation.
IMMUNOLOGY LETTERS
(2021)
Article
Biochemistry & Molecular Biology
Man-Ling Zhang, Yong Jin, Li-Hua Zhao, Jia Zhang, Meng Zhou, Mei-Shuang Li, Zhi-Bao Yin, Zi-Xin Wang, Li-Xia Zhao, Xi-He Li, Rong-Feng Li
Summary: Researchers have successfully derived porcine XEN cells through a novel method, showing positive XEN markers in vivo and retaining the ability to undergo VE and PE differentiation in vitro. These cells can be stably passaged and are significant for studying porcine embryo development and differentiation pathways.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Engineering, Industrial
Julia Mindlina, Horst Tempelmeier
Summary: This study focuses on stochastic flow lines with limited material supply, where shortages of material supply can limit system performance. By using linear programming approaches, the impact of material shortages on system output is quantified, and optimal material levels are determined to prevent shortages and optimize system performance. Insights are gained on the accuracy of linear programs and the dependence of optimal material levels on flow line characteristics.
INTERNATIONAL JOURNAL OF PRODUCTION RESEARCH
(2022)
Article
Construction & Building Technology
Sara Reichenbach, Benjamin Kromoser
Summary: The current evaluation of the building industry reveals low efficiency in material use and automation level, as well as a scarcity of skilled workers in mechanical engineering and construction. Therefore, there is a noticeable shift towards automated production processes, particularly in the field of precast concrete construction. The high innovation spirit in individual research facilities and companies has led to various new applications of robotics and automated systems in the precast industry.
JOURNAL OF BUILDING ENGINEERING
(2021)
Article
Medicine, Research & Experimental
Somayeh Naderi, Zahra Shiri, Masoumeh Zarei-Kheirabadi, Sepideh Mollamohammadi, Parastoo Hosseini, Golnoosh Rahimi, Azadeh Moradmand, Azam Samadian, Amir Shojaei, Meghdad Yeganeh, Seyed Ahmad Mousavi, Motahare Badri, Adeleh Taei, Seyedeh-Nafiseh Hassani, Hossein Baharvand
Summary: This study aimed to generate transplantable midbrain dopaminergic (mDA) progenitors for treatment of Parkinson's Disease (PD). By optimizing the differentiation protocol using clinical-grade human embryonic stem cells (hESC), the researchers successfully obtained functional mDA progenitors. Transplantation of cryopreserved mDA progenitors into rats with 6-hydroxydopamine lesions resulted in functional recovery. This study provides a foundation for future translational applications.
Article
Biochemistry & Molecular Biology
Dan Xing, Kai Wang, Jun Wu, Yu Zhao, Wei Liu, Jiao Jiao Li, Tingting Gao, Deng Yan, Liu Wang, Jie Hao, Jianhao Lin
Summary: The study demonstrates that multiple injections of ES-MSCs can improve osteoarthritis in a rat model. The repeated doses group showed more proteoglycan and less cartilage loss, along with lower Mankin scores, indicating the potential of ES-MSCs as a regenerative therapy for OA.
Article
Biochemical Research Methods
N. A. Willoughby, H. Bock, M. A. Hoeve, S. Pells, C. Williams, G. McPhee, P. Freile, D. Choudhury, P. A. De Sousa
Article
Endocrinology & Metabolism
Margaret R. Davis, Erik Arner, Cairnan R. E. Duffy, Paul A. De Sousa, Ingrid Dahlman, Peter Arner, Kim M. Summers
MOLECULAR GENETICS AND METABOLISM
(2016)
Editorial Material
Cell & Tissue Engineering
P. A. De Sousa, B. J. Tye, K. Bruce, P. Dand, G. Russell, D. M. Collins, A. Greenshields, K. McDonald, H. Bradburn, A. Laurie, J. M. Downie, M. Bateman, A. Courtney
STEM CELL RESEARCH
(2016)
Editorial Material
Cell & Tissue Engineering
P. A. De Sousa, B. J. Tye, K. Bruce, P. Dand, G. Russell, D. M. Collins, A. Greenshields, K. McDonald, H. Bradburn, A. Laurie, J. M. Downie, M. Bateman, A. Courtney
STEM CELL RESEARCH
(2016)
Editorial Material
Cell & Tissue Engineering
P. A. De Sousa, B. Tye, K. Bruce, P. Dand, G. Russell, J. Gardner, J. M. Downie, M. Bateman, A. Courtney
STEM CELL RESEARCH
(2016)
Editorial Material
Cell & Tissue Engineering
P. A. De Sousa, B. J. Tye, S. Sneddon, K. Bruce, P. Dand, G. Russell, D. M. Collins, A. Greenshields, K. McDonald, H. Bradburn, J. Gardner, J. M. Downie, A. Courtney, D. R. Brison
STEM CELL RESEARCH
(2016)
Editorial Material
Cell & Tissue Engineering
P. A. De Sousa, B. J. Tye, D. M. Collins, K. Bruce, P. Dand, G. Russell, H. Bradburn, J. M. Downie, M. Bateman, A. Courtney
STEM CELL RESEARCH
(2016)
Editorial Material
Cell & Tissue Engineering
P. A. De Sousa, B. J. Tye, K. Bruce, P. Dand, G. Russell, D. M. Collins, A. Greenshields, H. Bradburn, J. M. Downie, M. Bateman, A. Courtney
STEM CELL RESEARCH
(2016)
Editorial Material
Cell & Tissue Engineering
P. A. De Sousa, B. J. Tye, K. Bruce, P. Dand, G. Russell, D. M. Collins, A. Greenshields, H. Bradburn, J. M. Downie, M. Bateman, A. Courtney
STEM CELL RESEARCH
(2016)
Editorial Material
Cell & Tissue Engineering
P. A. De Sousa, B. J. Tye, K. Bruce, P. Dand, G. Russell, D. M. Collins, H. Bradburn, J. Gardner, J. M. Downie, M. Bateman, A. Courtney
STEM CELL RESEARCH
(2016)
Editorial Material
Cell & Tissue Engineering
P. A. De Sousa, B. J. Tye, K. Bruce, P. Dand, G. Russell, D. M. Collins, A. Greenshields, K. McDonald, H. Bradburn, A. Laurie, J. M. Downie, M. Bateman, A. Courtney
STEM CELL RESEARCH
(2016)
Article
Cell & Tissue Engineering
Paul A. De Sousa, Rachel Steeg, Elisabeth Wachter, Kevin Bruce, Jason King, Marieke Hoeve, Shalinee Khadun, George McConnachie, Julie Holder, Andreas Kurtz, Stefanie Seltmann, Johannes Dewender, Sascha Reimann, Glyn Stacey, Orla O'Shea, Charlotte Chapman, Lyn Healy, Heiko Zimmermann, Bryan Bolton, Trisha Rawat, Isobel Atkin, Anna Veiga, Bernd Kuebler, Blanca Miranda Serano, Tomo Saric, Juergen Hescheler, Oliver Bruestle, Michael Peitz, Cornelia Thiele, Niels Geijsen, Bjorn Holst, Christian Clausen, Majlinda Lako, Lyle Armstrong, Shailesh K. Gupta, Alexander J. Kvist, Ryan Hicks, Anna Jonebring, Gabriella Brolen, Andreas Ebneth, Alfredo Cabrera-Socorro, Patrik Foerch, Martine Geraerts, Tina C. Stummann, Shawn Harmon, Carol George, Ian Streeter, Laura Clarke, Helen Parkinson, Peter W. Harrison, Adam Faulconbridge, Luca Cherubin, Tony Burdett, Cesar Trigueros, Minal J. Patel, Christa Lucas, Barry Hardy, Rok Predan, Joh Dokler, Maja Brajnik, Oliver Keminer, Ole Pless, Philip Gribbon, Carsten Claussen, Annette Ringwald, Beate Kreisel, Aidan Courtney, Timothy E. Allsopp
STEM CELL RESEARCH
(2017)
Editorial Material
Biotechnology & Applied Microbiology
Paul A. De Sousa, Rachel Steeg, Beate Kreisel, Timothy E. Allsopp
TRENDS IN BIOTECHNOLOGY
(2017)
Review
Clinical Neurology
Paul A. De Sousa, Diane Ritchie, Alison Green, Siddharthan Chandran, Richard Knight, Mark W. Head
ACTA NEUROPATHOLOGICA
(2019)
Article
Cell & Tissue Engineering
Eirini Koutsouraki, Steve Pells, Paul A. De Sousa
STEM CELL RESEARCH
(2019)
