Journal
SPINAL CORD
Volume 54, Issue 10, Pages 778-784Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/sc.2016.20
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Funding
- National Natural Science Foundation of China [81201403, 81171114]
- Scientific Research Project of Fujian Provincial Department of Education [JB12093]
- Scientific Research Project of Quanzhou City Administration of Science and Technology [2012Z31]
- Special Fund for Training Outstanding Talents of Quanzhou City
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Study design: We introduced an adenoviral vector expressing interleukin-1 beta (IL-1 beta) small-hairpin RNA (shRNA) into the injured spinal cords to evaluate the therapeutic potential of IL-1 beta downregulation in a rat model of spinal cord injury (SCI). Objectives: The purpose of this study was to investigate the possible protective effects of the IL-1 beta downregulation on traumatic SCI in rats. Setting: Department of Orthopedic Surgery, The Second Affiliated Hospital, Fujian Medical University, Quanzhou, People's Republic of China. Methods: An adenoviral shRNA targeting IL-1 beta was constructed and injected at the T12 section 7 days before SCI. The rats' motor functions were evaluated by the Basso-Beattie-Bresnahan (BBB) rating scale. Immunofluorescence, enzyme-linked immunosorbent assay, flow-cytometric analysis and western blots were also performed. Results: Animals downregulating IL-1 beta had significantly better recovery of locomotor function and less neuronal loss after SCI. In addition, IL-1 beta downregulation significantly decreased tumor necrosis factor-alpha (TNF-alpha) level and Bax expression, reduced the activity of caspase-3 and increased Bcl-2 expression after SCI. Conclusion: This study demonstrated that the IL-1 beta downregulation may have potential therapeutic benefits for both reducing secondary damages and improving the outcomes after traumatic SCI.
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