Journal
SOUTH AFRICAN JOURNAL OF BOTANY
Volume 102, Issue -, Pages 12-17Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.sajb.2015.08.003
Keywords
Endangered; Ex vitro; Leaf regeneration; Vegetable rennet and withanolides
Categories
Funding
- Council of Scientific and Industrial Research (CSIR), New Delhi
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Withania coagulans (Stocks) Dunal (Solanaceae) is a critically endangered medicinal plant known for its multiple medicinal properties. We report here an efficient and high frequency plantlet regeneration system through direct organogenesis in W. coagulans using in vitro-derived leaves as explants. On Murashige and Skoog's (MS) medium containing 4.44 mu M 6-benzylaminopurine (BAP) 73.7 +/- 43% explants responded and produced 11.4 +/- 0.9 shoot buds per explant. The regenerated shoot buds were elongated (6.7 +/- 0.22 cm) on MS medium with 1.11 mu M BAP and 057 mu M indole-3-acetic acid (IAA). The elongated shoots were rooted both in vitro and ex vitro. The regenerates were acclimatized by slow and gradual exposure to different regimes of temperature and relative humidity. The leaf-regenerates were assessed for genetic stability using RAPD and ISSR molecular markers and confirmed true-to-type. The in vitro regeneration system developed would be useful for genetic restoration program as the overexploitation and reproductive failure forced W coagulans towards the verge of complete extinction. The regeneration method would also be useful for genetic transformation for genetic improvement and improved phyto-pharming. (C) 2015 SAAB. Published by Elsevier B.V. All rights reserved.
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