Journal
SOIL BIOLOGY & BIOCHEMISTRY
Volume 96, Issue -, Pages 176-179Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.soilbio.2016.02.001
Keywords
Nitrogen use efficiency; Rhizosphere; Protease activity; Protease encoding genes; Illumina sequencing; PCR-DGGE
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Funding
- Ministry of Education under Research project 'PRIN' [2009MWY5F9']
- Marie Curie ITN action 'TRAINBIODIVERSE' [289949]
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This study was carried out to understand the interplay of plant Nitrogen Utilizing Efficiency (NUE) with protease activity and microbial proteolytic community composition in the rhizosphere and bulk soils. Protease activity, diversity and abundance of protease genes (using DGGE and qPCR respectively of two key bacterial protease encoding genes: alkaline metallo-peptidase (apr) and neutral-metallopeptidases (npr)) were monitored in both rhizosphere and bulk soils from two maize in-bred lines L05 and T250 with higher and lower NUE respectively, using a rhizobox approach. Illumina sequencing was employed to assess the diversity of proteolytic communities encoding for the above-mentioned protease genes. Our results show higher enzyme activity, higher abundance and diversity of proteolytic genes in L05 maize rhizosphere, with higher NUE than in T250 maize rhizosphere. (C) 2016 Elsevier Ltd. All rights reserved.
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