Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 229, Issue -, Pages 100-109Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2016.01.114
Keywords
Quantum dots; Acetamiprid; Aptamer; Quantification; Imaging
Funding
- National Natural Science Foundation of China [21275056, 21575043, 51478196]
- Natural Science Foundation of Guangdong province [10251063101000000]
- cultivation foundation of South China Normal University for young teachers [14KJ08]
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In this paper, a novel turn-on sensor was constructed for quantification and imaging of acetamiprid. Acetamiprid aptamer-modified ZnS:Mn probe (ZnS:Mn-Aptamer) was obtained by conjugating ZnS:Mn QDs with acetamiprid binding aptamer. The fluorescence of the probe was turn off by multi-walled carbon nanotubes (MWCNTs) based on fluorescence resonance energy transfer (FRET) between ZnS: Mn-Aptamer and MWCNTs. After acetamiprid was introduced, the fluorescence was turn on because ZnS:Mn-Aptamer specifically binded with acetamiprid. Based on the above-mentioned principle, the quantitative detection and imaging of acetamiprid in real samples were achieved. The linear equation could be described as F/F-0 = 1.101 + 0.0312C(A) (C-A represented the concentration of acetamiprid, nM) in a linear range of 0-150 nM with a detection limit of 0.7 nM. The results suggested this method had remarkable sensitivity, selectivity, and was very simple without complex operation. Meanwhile, this novel turn-on sensor has the potential of in situ visual determination for pesticide residues in complex system. (C) 2016 Elsevier B.V. All rights reserved.
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