Article
Nutrition & Dietetics
Kristina Arih, Nina Dordevic, Mitja Kosnik, Matija Rijavec
Summary: Histamine intolerance is a clinical condition caused by decreased activity of the enzyme diamine oxidase, leading to histamine accumulation. Measurement of serum DAO can aid in the diagnosis of HIT, but should not be the sole diagnostic tool.
Article
Nutrition & Dietetics
Lucas Kettner, Ines Seitl, Lutz Fischer
Summary: A new diamine oxidase (DAO-1) was discovered in yeast, which has the potential to treat histamine intolerance by oral supplementation under simulated intestinal conditions.
Article
Nutrition & Dietetics
Sonia Sanchez-Perez, Oriol Comas-Baste, Judit Costa-Catala, Irache Iduriaga-Platero, M. Teresa Veciana-Nogues, M. Carmen Vidal-Carou, M. Luz Latorre-Moratalla
Summary: There are uncertainties regarding the onset of histamine adverse effects that still require further research. This study investigated the interference of different amines on the rate of histamine degradation by the enzyme DAO. The results showed that certain amines significantly delayed histamine degradation, while others inhibited it, suggesting a possible explanation for the variable susceptibility to dietary histamine.
FRONTIERS IN NUTRITION
(2022)
Article
Nutrition & Dietetics
Valentina Cucca, Giuseppe A. Ramirez, Patrizia Pignatti, Chiara Asperti, Marco Russo, Emanuel Della-Torre, Daniela Breda, Samuele E. Burastero, Lorenzo Dagna, Mona-Rita Yacoub
Summary: This study aimed to evaluate the clinical usefulness and consistency of serum DAO value ranges and verify their predictive value for treatment response in HIT patients. The results showed that the severity of symptoms in HIT patients was correlated with the degree of DAO deficiency. Patients with DAO values between 3 and 10 U/mL showed the best response to treatment.
Article
Chemistry, Applied
Xinxin Tian, Haozhe Liu, Huan Liu, Xiaodong Wang
Summary: In this study, we developed a biosensor based on phase-change microcapsules for detecting histamine in foods at high temperatures. The biosensor exhibited higher response sensitivity and lower detection limit through temperature regulation.
Review
Biotechnology & Applied Microbiology
Lucas Kettner, Ines Seitl, Lutz Fischer
Summary: Consuming foods with high histamine levels can cause allergy-like symptoms if the body cannot sufficiently break down histamine. This can lead to histamine intolerance. One solution is to produce histamine-free or reduced foods and supplement with exogenous DAO. Microbial DAOs show promise due to their efficient biotechnological production capabilities, but their biochemical properties are important for successful application.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Lucas Kettner, Carina Braun, Ines Seitl, Eva Pross, Lutz Fischer
Summary: In this study, a putative diamine oxidase (DAO) from Yarrowia lipolytica was successfully recombinantly integrated into the genome of Y. lipolytica for production in a bioreactor, showing a 93-fold increase in specific DAO activity compared to the native production. The produced DAO-1 exhibited broad substrate selectivity, with tyramine, histamine, putrescine, and cadaverine being the favored substrates, making it a potentially valuable enzyme for biogenic amine degradation in the food industry.
JOURNAL OF BIOTECHNOLOGY
(2021)
Article
Medicine, General & Internal
Elena Garcia-Martin, Santiago Navarro-Munoz, Gemma Amo, Christopher Rodriguez, Mercedes Serrador, Hortensia Alonso-Navarro, Marisol Calleja, Laura Turpin-Fenoll, Marta Recio-Bermejo, Rafael Garcia-Ruiz, Jorge Millan-Pascual, Francisco Navacerrada, Jose Francisco Plaza-Nieto, Esteban Garcia-Albea, Jose A. G. Agundez, Felix Javier Jimenez-Jimenez
Summary: This study investigated the relationship between serum diamine oxidase (DAO) activity and histamine levels, as well as three polymorphisms in the DAO gene, and the risk for migraine. The results showed that serum DAO activity was significantly higher in migraine patients, especially in females, while serum histamine levels were similar in both study groups. None of the studied polymorphisms were associated with the risk for migraine.
EUROPEAN JOURNAL OF CLINICAL INVESTIGATION
(2022)
Article
Chemistry, Analytical
Xia Xu, Xiaotian Wu, Shunqian Zhuang, Yucong Zhang, Yuting Ding, Xuxia Zhou
Summary: In this study, magnetic graphene oxide was used to immobilize diamine oxidase for the development of a colorimetric biosensor to detect histamine in fish. The biosensor showed a strong response to histamine and had negligible interference from other compounds. The etching induced multicolor change of the gold nanorods indicated the presence of varying levels of histamine in the fish, which was consistent with the overall change in the content of total volatile basic nitrogen. This colorimetric biosensor has great potential in evaluating the freshness of red-fleshed fish high in histamine.
Review
Chemistry, Physical
Zhaowang Tan, Yingwei Ou, Wenwei Cai, Yueliang Zheng, Hengjie Li, Yunyun Mao, Shengang Zhou, Jianfeng Tu
Summary: The serum level of diamine oxidase (DAO) is crucial in diagnosing various diseases and reflects the integrity and maturation of the small intestinal mucosa. This review summarizes previous studies on DAO level changes in diverse diseases, its clinical applications, and its roles under physiological and pathological conditions. The advances in understanding the mechanism and clinical applications of DAO presented in this review will contribute to future research and applications.
Article
Biochemical Research Methods
Isabel Sanz-Vicente, Irina Rivero, Lucia Marcuello, Maria Pilar Montano, Susana de Marcos, Javier Galban
Summary: Tyramine oxidase (TAO), peroxidase (HRP), and Amplex Red (AR) were immobilized on cellulose to create disposable biosensors for histamine determination. The enzymatic reaction produced a pink spot through the oxidation of AR. By using a smartphone to measure the G (green) color coordinate, histamine can be quantified in the presence of other biogenic amines in a range of concentrations, with a low limit of detection. The method was successfully applied to determine tyramine and histamine in a tuna extract, showing good results compared to the reference HPLC-MS method.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
P. Ramu, S. P. Vimal, P. Suresh, Anandhavelu Sanmugam, U. Saravanakumar, Raju Suresh Kumar, Abdulrahman I. Almansour, Natarajan Arumugam, Dhanasekaran Vikraman
Summary: The PEDOT-GO-PPO sensor was fabricated for dopamine detection, with the morphology and oxidation-reduction characteristics studied for optimization. Analysis of the detection limit and linear range data showcased the performance advantages of the modified electrode.
Article
Biology
Elisabeth Gludovacz, Kornelia Schuetzenberger, Marlene Resch, Katharina Tillmann, Karin Petroczi, Markus Schosserer, Sigrid Vondra, Serhii Vakal, Gerald Klanert, Jurgen Pollheimer, Tiina A. Salminen, Bernd Jilma, Nicole Borth, Thomas Boehm
Summary: By mutating the heparin-binding motif of hDAO, the plasma clearance rate was successfully reduced in rodents, showing potential for treating diseases characterized by high plasma histamine concentrations.
Article
Biochemistry & Molecular Biology
Meriem Megoura, Pompilia Ispas-Szabo, Mircea Alexandru Mateescu
Summary: This study formulated gastro-resistant tablets containing vegetal diamine oxidase (vDAO) as a treatment for histamine-related intestinal disorders. The use of sucrose and trehalose as cryoprotectants was found to protect vDAO from freeze-drying stress and improve the release performance of the tablets.
Article
Chemistry, Physical
Bin Li, Xingmin Wu, Chaosheng Shi, Yongqiang Dai, Jingfei Zhang, Wanyu Liu, Chen Wu, Yangfan Zhang, Xue Huang, Wei Zeng
Summary: The development of flexible glucose biosensors for non-invasive blood glucose monitoring through wearable devices is significant for diabetes prevention and treatment. A flexible GCGP biosensor was fabricated for sweat glucose detection, exhibiting high sensitivity and low detection limit. The biosensor showed high selectivity for glucose and good response in real sweat, attributed to the inclusion of GS.
SURFACES AND INTERFACES
(2023)
Review
Biochemistry & Molecular Biology
Ancuta Dinu, Constantin Apetrei
Summary: In recent years, the development of sensors and biosensors has become a hot topic in interdisciplinary research, attracting researchers from various fields. This paper synthesizes the most relevant studies on the construction and functioning of versatile devices, as well as electrochemical sensors and biosensors. It focuses on the role and importance of the amino acids phenylalanine, tyrosine, and tryptophan in the central nervous system, and the application of conductor polymers and molecularly imprinted polymers in achieving electrochemical sensors and biosensors. The paper also analyzes the performance of sensors and biosensors developed so far for amino acid detection.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Polymer Science
Ancuta Dinu, Constantin Apetrei
Summary: This study presents the development of a new enzyme sensor for the quantification of tyrosine in pharmaceuticals. The enzyme sensor showed a low limit of detection and high sensitivity, and was validated using infrared spectrometry on pharmaceuticals from different manufacturers.
Article
Chemistry, Physical
Irina Georgiana Munteanu, Constantin Apetrei
Summary: The purpose of this research was to develop a new enzymatic biosensor for sensitive detection of chlorogenic acid (CGA). The modified electrode showed a great catalytic effect on the electrochemical redox of CGA, leading to a linear peak current response. The detection limit and quantification limit were determined, and the method proved suitable for the detection of CGA in nutraceutical formulations. The correlation between the voltammetric method and the spectrophotometric one was good, and the antioxidant activity of CGA was demonstrated.
Article
Biochemistry & Molecular Biology
Irina Georgiana Munteanu, Constantin Apetrei
Summary: The screening and quantification of antioxidants from biological samples is essential for human health, and electrochemical sensors and biosensors show promising potential in antioxidant research, providing fast, economical, and reliable analytical methods for their detection.
Article
Chemistry, Analytical
Ancuta Dinu, Constantin Apetrei
Summary: This study reports the determination of ascorbic acid using platinum-based voltammetric sensors modified with potassium hexacyanoferrate-doped polypyrrole. The modified electrodes showed excellent sensitivity for ascorbic acid detection, with a detection limit of 2.5×10(-7) M. The analyses of pharmaceutical products with different concentrations of ascorbic acid validated the accuracy of the method.
Article
Biochemistry & Molecular Biology
Irina Georgiana Munteanu, Constantin Apetrei
Summary: The analysis of antioxidants in different foodstuffs has been a popular area of research, leading to the development of new antioxidant assays. Green tea, known for its health benefits, contains catechin, an important antioxidant. This study investigates the use of different electrodes and enzyme modifications to measure the catechin content and antioxidant activity in green tea.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Alexandra Virginia Bounegru, Constantin Apetrei
Summary: Hydroxytyrosol (HT) is an important marker for authenticity and quality assessment of extra virgin olive oils (EVOO). This study developed a biosensor based on a screen-printed electrode modified with single-layer carbon nanotubes and tyrosinase (SPE-SWCNT-Ty) for the qualitative and quantitative determination of hydroxytyrosol in commercial EVOOs. The results showed that the biosensor exhibited increased selectivity and sensitivity, making it suitable for quality control of EVOOs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Analytical
Alexandra Virginia Bounegru, Constantin Apetrei
Summary: This study aimed to simultaneously qualitatively and quantitatively determine two hydroxycinnamic acids using a carbon nanofiber-based screen-printed sensor. The method was found to be stable, reproducible, and sensitive, making it suitable for detecting these compounds in various dietary supplements or pharmaceutical products.
Article
Biochemistry & Molecular Biology
Alexandra Virginia Bounegru, Constantin Apetrei
Summary: This study achieved qualitative and quantitative determination of oleuropein in commercial EVOO samples using electrochemical techniques. Enzyme biosensors were constructed with successful immobilization of enzymes on carbon nanotube-modified electrodes, demonstrating efficient detection of oleuropein. The tyrosinase-based biosensor showed superior sensitivity towards oleuropein compared to the laccase-based one.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Multidisciplinary
Irina Georgiana Munteanu, Vasile Robert Gradinaru, Constantin Apetrei
Summary: Peptides have been used as components in biological analysis and fabrication of novel sensors due to their synthesis protocols and diverse structures. This study presents the development of a peptide-based sensor with improved performance for the quantification of rosmarinic acid in cosmetic products.
Article
Chemistry, Analytical
Dorin Dascalescu, Constantin Apetrei
Summary: A new Lac-based biosensor has been developed to qualitatively and quantitatively determine serotonin in dietary supplements. The biosensor showed selective detection capabilities and superior electrochemical performance. The quantitative determination of serotonin by square-wave voltammetry showed good correlation with the FTIR method.
Article
Biochemistry & Molecular Biology
Irina Georgiana Munteanu, Vasile Robert Gradinaru, Constantin Apetrei
Summary: Olive phenolic compounds have antioxidant and antimicrobial properties in functional foods, beverages, and dermato-cosmetic products. They are also recognized for their role in preventing diabetes, inflammation, and heart disease. This study developed a new sensor to detect verbascoside in commercial extra virgin olive oils (EVOOs) by modifying a carbon electrode with graphene oxide. The sensor showed high sensitivity, low detection limit, and a wide linearity range. The sensor successfully detected verbascoside in different EVOO samples, and the method was validated with good results.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Elisabeta-Irina Geana, Corina Teodora Ciucure, Irina Mirela Apetrei, Maria Lisa Clodoveo, Constantin Apetrei
Summary: This study presents a methodology for distinguishing olive oil and extra-virgin olive oil from other vegetable oils using targeted and untargeted high-resolution mass spectrometry (HRMS) analysis of phenolic and triterpenic compounds, coupled with multivariate statistical analysis. Certain phenolic compounds, coumaric acids, apigenin, pinocembrin, hydroxytyrosol, and maslinic acid, were found to be potential biomarkers of olive oil, with higher levels present in extra-virgin olive oil compared to other vegetable oils. Principal component analysis (PCA) and heat map profiles based on the HRMS data were able to discriminate olive oils from other vegetable oils. This methodology could be applied for authenticity and classification purposes, considering factors such as variety, geographical origin, and adulteration practices.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Irina Georgiana Munteanu, Constantin Apetrei
Summary: The classification and authentication of olive oils based on their biological or geographic origin is crucial for public health and the market. This study explored the use of cyclic voltammograms (CVs) obtained from electrochemical sensors to discriminate and classify vegetable oils. The results show that this method enables clear differentiation of oils from different origins.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Chemistry, Multidisciplinary
Alexandra Virginia Bounegru, Constantin Apetrei
Summary: This review examines the various tyrosinase immobilization techniques for the development of electrochemical biosensors. Cross-linking immobilization is deemed the most feasible and economical approach, with advantages such as enhanced biosensor performance characteristics and the ability to select reagents and immobilization steps. Recent research has also focused on new immobilization strategies, including cross-linked enzyme aggregates (CLEAs) and magnetic cross-linked enzyme aggregates (mCLEAs).