Article
Biochemistry & Molecular Biology
Martina Leitner, Christian Buchold, Ralf Pasternack, Nikolaus B. Binder, Gary W. Moore
Summary: The study validated a new FXIII activity detection method that is rapid, accurate, and precise for routine clinical use, with fewer interferents than ammonia release FXIII activity detection methods.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Chiara Motta, Angelica Pellegrini, Stefano Camaione, Joan Geoghegan, Pietro Speziale, Giulia Barbieri, Giampiero Pietrocola
Summary: In this study, the interaction between the secreted von Willebrand factor-binding protein (vWbp) from Staphylococcus aureus and coagulation factors prothrombin and fibrinogen (Fbg) was investigated. It was found that vWbp-activated Factor XIII (FXIII) catalyzes the incorporation of amino-donor dansylcadaverine into region A of fibronectin-binding protein A (FnBPA). The study suggests a novel mechanism by which transglutaminase action of FXIII and TG2 contributes to the entrapment and persistence of S. aureus in blood and host tissues.
SCIENTIFIC REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Fahad S. M. Alshehri, Claire S. Whyte, Ahmet Tuncay, Maria-Louise Williams, Heather M. Wilson, Nicola J. Mutch
Summary: FXIII is a transglutaminase that promotes thrombus stability by cross-linking fibrin. Monocytes and THP-1 cells exhibit up-regulation of FXIII-A antigen and activity in response to IL-4 and IL-10 stimulation, leading to enhanced stability of thrombi against fibrinolytic degradation. Membrane-associated FXIII-A in monocytes appears to play a crucial role in thrombus stabilization, particularly through a transglutaminase-dependent mechanism.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Oncology
Robert Tempest, Sonia Guarnerio, Rawan Maani, Jamie Cooper, Nicholas Peake
Summary: Transglutaminase-2 (TG2) is an enzyme involved in protein cross-linking and has been linked to the development and progression of various cancers. It plays a role in cellular processes related to apoptosis, chemoresistance, epithelial-mesenchymal transition, and stem cell phenotype. TG2 influences the tumour microenvironment by modulating matrix remodelling, biomechanical properties, cell adhesion, motility, and invasion. Therapeutic targeting of TG2 in the TME shows promise, with ongoing efforts in drug development and clinical trials aiming to target its biomolecular and biomechanical function.
Review
Biochemistry & Molecular Biology
Michal Zabczyk, Joanna Natorska, Anetta Undas
Summary: FXIII is crucial in the modulation of fibrin clot properties in patients with VTE, impacting clot structure and function. FXIII activity is significantly reduced in acute PE and DVT patients, but gradually increases after the acute phase. Reduced FXIII activity is associated with impaired clot permeability and hypofibrinolysis in acute PE patients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Hematology
Akitada Ichinose, Tsukasa Osaki, Masayoshi Souri
Summary: Autoimmune factor XIII deficiency is a rare and life-threatening bleeding disorder that primarily affects the elderly. Research shows that patients with AiF13D have lower levels of FXIII antigen, and specific screening tests can effectively differentiate this condition from others.
Review
Biochemistry & Molecular Biology
Fahad S. M. Alshehri, Claire S. Whyte, Nicola J. Mutch
Summary: Factor XIII is a transglutaminase enzyme crucial for hemostasis, with deficiency leading to severe bleeding diathesis. FXIII exists in different forms in plasma and cells, playing important roles in physiological processes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Thomas Schmitz, Ajay Abisheck Paul George, Britta Nubbemeyer, Charlotte A. Bauml, Torsten Steinmetzer, Oliver Ohlenschlager, Arijit Biswas, Diana Imhof
Summary: The saliva of blood-sucking leeches contains anticoagulant substances, including tridegin which can inhibit blood coagulation factor FXIIIa. Synthesized tridegin variants have potential for antithrombotic therapy, and structural analysis using NMR spectroscopy and MD simulations can provide insights into structure-activity relationships for further lead optimization. This study supports the development of anticoagulant therapy targeting FXIIIa.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Anesthesiology
Patricia Guilabert, Lars Asmis, Vicente Cortina, Joan P. Barret, Maria J. Colomina
Summary: Factor XIII (FXIII) is the final factor in the coagulation cascade and its deficiency can lead to surgical bleeding. Congenital FXIII deficiency is rare, while acquired FXIII deficiency is more common. This review provides guidance on the diagnosis and treatment of FXIII deficiency in preoperative consultation and surgical settings.
MINERVA ANESTESIOLOGICA
(2022)
Article
Hematology
Tsukasa Osaki, Chikako Yokoyama, Yasuo Magari, Masayoshi Souri, Akitada Ichinose
Summary: This study generated rat monoclonal antibodies against human FXIII-B and used them to create an immunochromatographic test (ICT) to detect anti-FXIII-B autoantibodies and distinguish between AiF13D and acF13D. The ICT showed similar precision to dot-blot analysis. Combining this ICT with the previously developed ICT for anti-FXIII-A autoantibodies may reduce false negatives and enable accurate diagnosis and treatment.
THROMBOSIS AND HAEMOSTASIS
(2023)
Review
Biochemistry & Molecular Biology
Vassilios P. Memtsas, Deepa R. J. Arachchillage, Diana A. Gorog
Summary: This review article discusses the pathophysiological role and laboratory assessment of fibrin, factor XIII, and endogenous fibrinolysis in the final phase of coagulation cascade and fibrinolysis, as well as their involvement in various disease states and assessment of cardiovascular risk with the most up-to-date evidence.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Lei Mao, Yiyang Huang, Weiqiang Jia, Zhi Zhou, Xiaobo He
Summary: This study investigated the relationship between the expression of matrix metalloproteinase 9 and 2 genes with cerebral hemorrhage in patients with coagulation factor XIII deficiency. The results showed that the increased expression of the MMP-9 gene may be related to the pathogenesis of cerebral hemorrhage.
CELLULAR AND MOLECULAR BIOLOGY
(2022)
Article
Hematology
Masayoshi Souri, Chikako Yokoyama, Tsukasa Osaki, Akitada Ichinose
Summary: Anti-FXIII-B antibodies can bind to the catalytic subunit of FXIII, thus inhibiting its activation and fibrin crosslinking function.
THROMBOSIS AND HAEMOSTASIS
(2023)
Article
Medicine, General & Internal
Nasim Shahidi Hamedani, Arijit Biswas, Oliver Rudan, Rosa Toenges, Carlotta Meyring, Fabian Tolle, Guenter Mayer, Johannes Oldenburg, Jens Mueller, Bernd Poetzsch
Summary: Six DNA aptamers specific for activated FXIII (FXIIIa) were selected, with FA12 capturing FXIIIa efficiently and inhibiting its incorporation with fibrinogen and alpha 2-antiplasmin. Another aptamer, FA2, also showed significant effects in plasma-based thromboelastometry analysis. The structure-function correlations revealed FA12 as a potential candidate for FXIIIa-targeting therapeutic strategies and diagnostic assays.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Hematology
Wayne L. Chandler
Summary: In patients undergoing extracorporeal membrane oxygenation (ECMO), coagulation is primarily activated through the contact system and formation of activated factor XIa, leading to increased thrombin generation potential (TGP) in plasma. Additionally, procoagulant phospholipids in the form of circulating platelet and red cell extracellular vesicles are increased in ECMO patients, which contribute to the elevation of TGP activity.
THROMBOSIS RESEARCH
(2022)
Letter
Hematology
Remi O. Kool, Hans P. Kohler, Jonathan M. Coutinho, Marcel Levi, Michiel Coppens, Joost C. M. Meijers, Verena Schroeder
THROMBOSIS RESEARCH
(2017)
Article
Multidisciplinary Sciences
Lorenz Jenny, Jozsef Dobo, Peter Gal, Gabor Pal, Wilbur A. Lam, Verena Schroeder
Letter
Hematology
Bojun Li, Munira Borhany, Madiha Abid, Hans P. Kohler, Verena Schroeder
THROMBOSIS RESEARCH
(2018)
Article
Hematology
Bojun Li, Ramya Billur, Muriel C. Maurer, Hans P. Kohler, Pascale Raddatz Mueller, Lorenzo Alberio, Verena Schroeder
THROMBOSIS AND HAEMOSTASIS
(2018)
Article
Hematology
Rolf P. Engelberger, Verena Schroeder, Michael Nagler, Raja Princes, Daniel Periard, Daniel Hayoz, Nils Kucher
THROMBOSIS AND HAEMOSTASIS
(2019)
Article
Hematology
Bojun Li, Hans P. Kohler, Verena Schroeder
Article
Biochemistry & Molecular Biology
Zsuzsanna Z. Orosz, Helga Bardos, Amir H. Shemirani, Ildiko Beke Debreceni, Riitta Lassila, Antti S. Riikonen, Johanna A. Kremer Hovinga, Theo G. Seiler, Hendrika A. van Dorland, Verena Schroeder, Zoltan Boda, Laszlo Nemes, Beatrice Frueh Eppstein, Bence Nagy, Andrea Facsko, Janos Kappelmayer, Laszlo Muszbek
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2019)
Article
Biochemistry & Molecular Biology
Lorenz Jenny, Danilo Noser, Julie Brogaard Larsen, Jozsef Dobo, Peter Gal, Gabor Pal, Verena Schroeder
MOLECULAR IMMUNOLOGY
(2019)
Article
Biochemistry & Molecular Biology
Ramzi A. Ajjan, Verena Schroeder
MOLECULAR IMMUNOLOGY
(2019)
Article
Endocrinology & Metabolism
Lorenz Jenny, Andreas Melmer, Markus Laimer, Elaissa T. Hardy, Wilbur A. Lam, Verena Schroeder
DIABETES & VASCULAR DISEASE RESEARCH
(2020)
Review
Hematology
Verena Schroeder
Article
Immunology
Murielle Golomingi, Jessie Kohler, Lorenz Jenny, Elaissa T. Hardy, Jozsef Dobo, Peter Gal, Gabor Pal, Bence Kiss, Wilbur A. Lam, Verena Schroeder
Summary: In this study, the role of MBL and MASP-1 in the haemostatic response after mechanical vessel injury was investigated using a microvascular bleeding model. MBL was found to recognize the injury site, while MASP-1 increased fibrin formation, platelet activation, and shortened bleeding time. These findings suggest that the complement lectin pathway has a beneficial role in the physiological coagulation response by supporting the crucial haemostatic system.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Murielle Golomingi, Jessie Kohler, Christina Lamers, Richard B. Pouw, Daniel Ricklin, Jozsef Dobo, Peter Gal, Gabor Pal, Bence Kiss, Arthur Dopler, Christoph Q. Schmidt, Elaissa Trybus Hardy, Wilbur Lam, Verena Schroeder
Summary: The potential role of complement components and activation during the haemostatic response to mechanical vessel injury was investigated in this study. The results suggest that complement inhibitors have an impact on reducing fibrin clot formation and platelet activation, indicating a role of the complement system in haemostasis.
FRONTIERS IN IMMUNOLOGY
(2023)
Review
Hematology
Sriram Krishnaswamy, Walter Ageno, Yaseen Arabi, Tiziano Barbui, Suzanne Cannegieter, Marc Carrier, Audrey C. Cleuren, Peter Collins, Laurence Panicot-Dubois, Jane E. Freedman, Kathleen Freson, Philip Hogg, Andra H. James, Colin A. Kretz, Michelle Lavin, Frank W. G. Leebeek, Weikai Li, Coen Maas, Kellie Machlus, Michael Makris, Ida Martinelli, Leonid Medved, Marguerite Neerman-Arbez, James S. O'Donnell, Jamie O'Sullivan, Madhvi Rajpurkar, Verena Schroeder, Paul Clinton Spiegel, Simon J. Stanworth, Laura Green, Anetta Undas
Summary: The 2021 Congress of the International Society of Thrombosis and Haemostasis (ISTH) was held virtually, showcasing cutting-edge advances in basic, population, and clinical sciences with 77 State of the Art talks covering a range of important topics related to hemostasis and thrombosis.
RESEARCH AND PRACTICE IN THROMBOSIS AND HAEMOSTASIS
(2021)