Journal
SCIENCE OF THE TOTAL ENVIRONMENT
Volume 541, Issue -, Pages 1243-1252Publisher
ELSEVIER
DOI: 10.1016/j.scitotenv.2015.10.003
Keywords
B[a]P; Case control study; DNA methylation; Epigenetic; GSTP; Hepatocellular carcinoma
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Funding
- National Natural Science Foundation of China foundation [21177123, 21307126]
- NSFC-RC International Exchange Programme
- Chinese Academy of Sciences (CAS)
- CAS/SAFEA International Partnership Program for Creative Research Teams [KZCX2-YW-T08]
- Xiamen Science and Technology Fund [3502Z20122003]
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Epidemiological studies implicate environmental risk factors and epigenetic alterations in the multistage process of hepatocellular carcinoma (HCC) development. However, associations between environmental factors and DNA methylation of tumour suppressor genes (TSGs) in HCC development remain ambiguous. Understanding how possible interactions influence risk may provide insights into the complexity of hepato-carcinogenesis. For this study, blood samples were collected from HCC patients (n = 90) and healthy volunteers (n = 99) from Xiamen (China) and data for selected environmental risk factors [e.g., benzo[a] pyrene (B[a]P), hepatitis B or C virus (HBV or HCV) infection, smoking and alcohol consumption] were recorded; factors identified as significantly higher (P < 0.05) amongst case subjects compared to controls were identified. In order to assess associations for epigenetic alterations and HCC risk factors, serum DNA methylation of TSGs was quantified using high-resolution melting (HRM) analysis. Our results clearly indicate elevated methylation patterns for detoxification gene [glutathione-S-transferase Pi (GSTP)] promoter regions in cases compared to control subjects. Additionally, GSTP promoter hypermethylation and B[a] P diol epoxide-albumin (BPDE-Alb) were positively correlated with HCC incidence. Our epidemiological and in vitro cell model studies indicated that GSTP promoter DNA methylation regulates this gene's expression. Moreover, GSTP also plays an important role in B[a]P detoxification and potential protective role against B[a] P-induced liver cell toxicity and hepato-carcinogenesis. (C) 2015 Elsevier B.V. All rights reserved.
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