Journal
RNA BIOLOGY
Volume 13, Issue 9, Pages 820-825Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/15476286.2016.1197481
Keywords
Full-length transcriptome; gene transcription; mitochondrial genes; PacBio; RNA processing
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Funding
- Nankai University
- Natural Science Foundation of China [31371974, 31201738]
- Fundamental Research Funds for the Central Universities
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In this study, we sequenced the first full-length insect transcriptome using the Erthesina fullo Thunberg based on the PacBio platform. We constructed the first quantitative transcription map of animal mitochondrial genomes and built a straightforward and concise methodology to investigate mitochondrial gene transcription, RNA processing, mRNA maturation and several other related topics. Most of the results were consistent with the previous studies, while to the best of our knowledge some findings were reported for the first time in this study. The new findings included the high levels of mitochondrial gene expression, the 3 polyadenylation and possible 5 m(7)G caps of rRNAs, the isoform diversity of 12S rRNA, the polycistronic transcripts and natural antisense transcripts of mitochondrial genes etal. These findings could challenge and enrich fundamental concepts of mitochondrial gene transcription and RNA processing, particularly of the rRNA primary (sequence) structure. The methodology constructed in this study can also be used to study gene expression or RNA processing of nuclear genomes.
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