Journal
RNA BIOLOGY
Volume 13, Issue 2, Pages 172-176Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/15476286.2015.1137420
Keywords
CRISPR; Cas9; D-repeat; long non-coding RNA; XIST; X-inactivation
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Funding
- National Natural Science Foundation of China [31201080, 31272394]
- Agricultural science and technology research of Guangdong province [2011A020102003]
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XIST is a long non-coding RNA, which expressed exclusively from the inactive X chromosome. Although it has been revealed that the A-repeat contributes to the X chromosome inactivation (X-inactivation), the role of the longest D-repeat has not yet been investigated. Here, a sgRNA directed CRISPR/Cas9 system which have multiple target sites within repeat D of XIST, were used to generate D-repeat deletion and studied its roles on X-inactivation. The results showed that the deletion of D-repeat caused a significantly decreased expression of XIST, and up regulated expression of X-linked genes, suggesting that the D-repeat may play an important role in the regulation of XIST expression and silencing of the X-linked genes, which could provide a new idea in the molecular mechanisms of X-inactivation.
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