Article
Multidisciplinary Sciences
C. L. Tschirhart, M. Serlin, H. Polshyn, A. Shragai, Z. Xia, J. Zhu, Y. Zhang, K. Watanabe, T. Taniguchi, M. E. Huber, A. F. Young
Summary: Researchers have found that electrons in moire flat band systems can break time-reversal symmetry, leading to a quantized anomalous Hall effect, with magnetism primarily orbital in nature. The study also reveals a significant change in magnetization as the chemical potential crosses the quantum anomalous Hall gap, consistent with the expected contribution of chiral edge states to the magnetization in an orbital Chern insulator. Additionally, mapping the spatial evolution of field-driven magnetic reversal shows reproducible micrometer-scale domains pinned to structural disorder.
Article
Biology
Jeremy Copperman, Sean M. Gross, Young Hwan Chang, Laura M. Heiser, Daniel M. Zuckerman
Summary: Time-lapse imaging is a powerful tool to study cell dynamics, but analyzing morphological changes over time is challenging. In this study, we use trajectory embedding to analyze cellular behavior based on multiple time points simultaneously. We apply this method to study the effects of microenvironmental perturbagens on MCF10A mammary epithelial cells. Our analysis reveals ligand-specific regulation of cell state transitions and provides descriptive models of single-cell trajectories. Incorporating trajectories improves the characterization of cell states and enables better identification of phenotypes compared to snapshot-based analysis. This approach has broad applications in live-cell imaging for studying cell responses.
COMMUNICATIONS BIOLOGY
(2023)
Article
Chemistry, Analytical
Su Liang, Zeyu Wang, Luyu Qi, Chao Tang, Yiming Zhang, Qun Luo, Yayun Wu, Jinghe Yuan, Yao Zhao, Yanyan Zhang, Xiaohong Fang, Shijun Wang, Fuyi Wang
Summary: This study designed a fluorescent probe, ATTO565-WGN, which showed similar anti-proliferative activity to wogonin against human cancer cell lines and demonstrated mitochondrial localization through confocal fluorescence imaging. The conjugated ATTO565 fluorophore enabled STED fluorescence living cell imaging with ultrahigh spatial resolution, facilitating the investigation of wogonin-biological target interactions in living cells at mitochondria. This work provides novel insights into rational design of mitochondrion targeting fluorescence probes for ultrahigh resolution living cell imaging.
Article
Chemistry, Multidisciplinary
Danyang Wang, Ana Shalamberidze, Emilia Arguello, Byron W. Purse, Ralph E. Kleiner
Summary: The article introduces a platform for fluorescence imaging of bulk RNA dynamics in living cells and uses this platform to study RNA metabolism and localization during oxidative stress. The study found that bulk RNA turnover is accelerated upon oxidative stress treatment and identified new cytoplasmic RNA granules that co-localize with RNA helicase.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Chemistry, Multidisciplinary
Dana Al Kelabi, Avishek Dey, Lukman O. Alimi, Hubert Piwonski, Satoshi Habuchi, Niveen M. Khashab
Summary: Fluorescent microscopy is a powerful technique for studying cellular dynamics. The organic fluorophore OC1, with high cell permeability, photostability, and selectivity for mitochondria, has been identified. The keto-enol tautomerization of OC1 allows for consistent fluorescence in dilute solutions through supramolecular self-assembly.
Article
Biotechnology & Applied Microbiology
Andreas Boden, Francesca Pennacchietti, Giovanna Coceano, Martina Damenti, Michael Ratz, Ilaria Testa
Summary: A new 3D pRESOLFT microscope method with sub-80-nm resolution has been developed to visualize the volumetric architecture of organelles and molecules inside whole living cells, allowing for targeted 3D confinement of fluorescence and observation of dynamic structural alterations in cells.
NATURE BIOTECHNOLOGY
(2021)
Review
Oncology
Maria Alieva, Amber K. L. Wezenaar, Ellen J. Wehrens, Anne C. Rios
Summary: Live-cell imaging provides a deeper understanding of cancer response to treatment by offering spatial, molecular, and morphological data over time. It has been applied to uncover tumour heterogeneity in treatment response and the mode of action of cancer-targeting drugs. The unique opportunity of time-lapse imaging in capturing the interactivity and motility of immunotherapies is also discussed. Recent technological advancements in multidimensional imaging and multi-omics data integration have allowed for the connection of single-cell dynamics to molecular phenotypes. These advancements significantly contribute to our understanding of tumour targeting and have implications for next-generation precision medicine.
NATURE REVIEWS CANCER
(2023)
Review
Optics
Herbert Schneckenburger, Verena Richter
Summary: This short overview discusses 3D live cell imaging, relevant samples, challenges like optical sectioning and phototoxicity, and enhanced microscopy methods with some examples and applications. Future advancements may involve increasing microscopic resolution and utilizing molecular sensing techniques.
Article
Nanoscience & Nanotechnology
Peilin Gu, Bin Chen, Tingting Zhai, Qian Li, Xiaolei Zuo, Lihua Wang, Anjun Qin, Yi Zhou, Jianlei Shen
Summary: The mechanical properties of soft luminescent nanoparticles have been explored for their efficiency in drug delivery and rapid elimination from cells. These soft AIE dots exhibit high light emission and can induce strong immunostimulatory effects, increasing the levels of proinflammatory cytokines. This study introduces a new design strategy for synthesizing a soft nanocarrier system that can efficiently deliver drugs into cells and be quickly eliminated.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Biology
Francesco Padovani, Benedikt Mairhoermann, Pascal Falter-Braun, Jette Lengefeld, Kurt M. Schmoller
Summary: Cell-ACDC is an open-source user-friendly framework written in Python for segmentation, tracking, and cell cycle annotations in live cell imaging data. It includes state-of-the-art deep learning models, visualization and error correction tools, and allows for fast integration of new methods.
Article
Multidisciplinary Sciences
George Alzeeb, Danielle Arzur, Valerie Trichet, Matthieu Talagas, Laurent Corcos, Catherine Le Jossic-Corcos
Summary: Gastric cancer is the third leading cause of cancer-related mortality worldwide, often diagnosed at advanced stages. Utilizing the cholesterol-lowering drug lovastatin as an adjuvant to chemotherapy has shown significant efficacy in treating gastric cancer. In a 3D model, the combination of lovastatin and docetaxel effectively eliminates growing gastric cancer cells.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Multidisciplinary
Lei Jiang, Hao Cai, Wanwan Zhou, Zijian Li, Liang Zhang, Hong Bi
Summary: Monitoring the dynamics of RNA granules and phase separation inside cells is important for understanding cellular responses under various stresses, such as oxidative stress. Current small-molecule RNA probes face limitations in stability and biocompatibility, highlighting the urgent need for RNA-targeting fluorescent nanoprobes that can be used in living cells. In this study, a novel RNA-targeting, red-emissive carbon dots (M-CDs) were designed and synthesized, which showed high water solubility, fluorescence quantum yield, and selectivity for RNA. The M-CDs were successfully internalized into cells within 5 seconds and were able to image the dynamic process of intracellular stress granules under oxidative stress in real-time. This research opens up new possibilities for visualizing bulk RNA dynamics and studying phase-separation behaviors in living cells.
ADVANCED MATERIALS
(2023)
Review
Plant Sciences
Maria Ada Prusicki, Martina Balboni, Kostika Sofroni, Yuki Hamamura, Arp Schnittger
Summary: Live-cell imaging is a powerful tool for understanding cellular processes, especially in meiosis where chromosomes and cellular components exhibit complex dynamics over a short period of time. Recent advances in live-cell imaging now allow real-time analysis of meiotic events in plants.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Chemistry, Multidisciplinary
Georgina C. Gavins, Katharina Groeger, Michael D. Bartoschek, Philipp Wolf, Annette G. Beck-Sickinger, Sebastian Bultmann, Oliver Seitz
Summary: DNA nanotechnology offers opportunities for manipulating and imaging proteins on cell surfaces by creating nucleic acid-protein junctions using PNA tags. This method allows for quick assembly of different fluorescent dyes, increased brightness, reversible labeling, and is applicable to various cell and protein systems.
Article
Chemistry, Multidisciplinary
Adam Coln Hundahl, Arjen Weller, Jannik Bruun Larsen, Claudia U. Hj, Morten B. Hansen, Ann-Kathrin Muendler, Astrid Knuhtsen, Kasper Kristensen, Eva C. Arnspang, Thomas Lars Andresen, Kim I. Mortensen, Rodolphe Marie
Summary: Oral drug delivery is the preferred administration route for most drugs, but it is limited for biologics due to the intestinal barrier. One strategy to improve the absorption is chemical modification through lipidation. However, the mechanistic understanding of the effect is largely unexplored. This study developed a method to quantify peptide transport through a monolayer of Caco-2 cells and investigated the effects of lipidation on transport mechanism.
JOURNAL OF CONTROLLED RELEASE
(2023)
Article
Biology
Mukulika Ray, Sundaram Acharya, Sakshi Shambhavi, Subhash C. Lakhotia
JOURNAL OF BIOSCIENCES
(2019)
Article
Biology
Anand K. Singh, Subhendu Roy Choudhury, Sandip De, Jie Zhang, Stephen Kissane, Vibha Dwivedi, Preethi Ramanathan, Marija Petric, Luisa Orsini, Daniel Hebenstreit, Saverio Brogna
Article
Anatomy & Morphology
Mukulika Ray, Subhash C. Lakhotia
DEVELOPMENTAL DYNAMICS
(2019)
Article
Developmental Biology
Mukulika Ray, Gunjan Singh, Subhash C. Lakhotia
GENE EXPRESSION PATTERNS
(2019)
Article
Biology
Anand K. Singh, Akilu Abdullahi, Matthias Soller, Alexandre David, Saverio Brogna
Article
Genetics & Heredity
Ranjan Kumar Sahu, Eshita Mutt, Subhash Chandra Lakhotia
JOURNAL OF GENETICS
(2020)
Article
Genetics & Heredity
Subhash C. Lakhotia
JOURNAL OF GENETICS
(2021)
Article
Biochemistry & Molecular Biology
Sandip De, David M. Edwards, Vibha Dwivedi, Jianming Wang, Wazeer Varsally, Hannah L. Dixon, Anand K. Singh, Precious O. Owuamalam, Matthew T. Wright, Reece P. Summers, Md Nazmul Hossain, Emily M. Price, Marcin W. Wojewodzic, Francesco Falciani, Nikolas J. Hodges, Marco Saponaro, Kayoko Tanaka, Claus M. Azzalin, Peter Baumann, Daniel Hebenstreit, Saverio Brogna
Summary: The study found that the RNA helicase Upf1 in Schizosaccharomyces pombe is associated with active genes genome-wide, correlates with Pol II transcription and mRNA expression levels, and may regulate gene expression by influencing Pol II phosphorylation and transcription.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Cell Biology
Gunjan Singh, Saptomee Chakraborty, Subhash C. Lakhotia
Summary: Heat shock proteins play important roles in the initiation and establishment of cancers. The major constitutively expressed heat shock proteins are involved in both early and late stages of epithelial tumors.
CELL STRESS & CHAPERONES
(2022)
Review
Biology
Anand Kumar Singh
Summary: Long noncoding RNAs (lncRNAs) play a crucial role in regulating gene expression at epigenetic, transcriptional, and posttranscriptional levels. Several regulatory lncRNAs, such as MALAT1, NEAT1, HOTAIR, are associated with neurodegenerative diseases in humans. Studies have shown that hsr omega lncRNAs derived from the hsr omega gene modulate neurotoxicity in Drosophila models of polyQ and amyotrophic lateral sclerosis diseases. Elevated expression of hsr omega lncRNAs exacerbates disease pathogenicity, while their genetic depletion suppresses it. This review discusses the mechanistic details and implications of hsr omega lncRNAs in neurodegenerative diseases.
Article
Immunology
Tanmayee Nayak, Anuja Kakkar, Rakesh Kumar Singh, Lav Kumar Jaiswal, Anand Kumar Singh, Louise Temple, Ankush Gupta
Summary: This article describes the isolation and characterization of a novel mycobacteriophage Kashi-VT1 (KVT1) infecting Mycobacterium smegmatis mc(2) 155 (M. smegmatis) and Mycobacterium fortuitum isolated from Varanasi, India. The KVT1 phage belongs to the Siphoviridae family and has a genome length of 61,010 base pairs with several characteristic genes, suggesting its potential therapeutic role against tuberculosis and other mycobacterial diseases.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Genetics & Heredity
Subhash C. Lakhotia
Summary: In the early 1980s, Hsp60 family of heat shock proteins (Hsp) were identified in various eukaryotes but not in Drosophila. However, in 1989, a putative Hsp60 family protein was discovered in the larval Malpighian tubules of Drosophila. Further studies revealed the presence of four putative Hsp60 gene sequences in the D. melanogaster genome. This historical perspective highlights the discovery and characterization of multiple Hsp60 genes in D. melanogaster.
JOURNAL OF GENETICS
(2022)
Article
Multidisciplinary Sciences
S. Chakraborty, J. Gowrishankar, A. Joshi, P. Kannan, R. K. Kohli, S. C. Lakhotia, G. Misra, C. M. Nautiyal, K. Ramasubramanian, N. Sathyamurthy, A. K. Singhvi
Article
Multidisciplinary Sciences
Praveen Chaddah, Subhash C. Lakhotia
PROCEEDINGS OF THE INDIAN NATIONAL SCIENCE ACADEMY
(2018)
Letter
Integrative & Complementary Medicine
Deep Narayan Pandey, Sanjeev Rastogi, Girdhar Gopal Agarwal, S. C. Lakhotia
JOURNAL OF AYURVEDA AND INTEGRATIVE MEDICINE
(2022)