Article
Chemistry, Analytical
Taiga Iwama, Kuniyuki Kano, Daisuke Saigusa, Kim Ekroos, Gerhild van Echten-Deckert, Johannes Vogt, Junken Aoki
Summary: The novel on-tissue derivatization method using Phos-tag in combination with MALDI-MSI enables visualization of low-abundance bioactive lipids like LPA and S1P in the brain, providing a simple and optimal way to reveal the spatial localization of these important molecules in tissues.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Mai H. Philipsen, Ellinor Hansson, Auraya Manaprasertsak, Stefan Lange, Eva Jennische, Helena Caren, Kliment Gatzinsky, Asgeir Jakola, Emma U. Hammarlund, Per Malmberg
Summary: Glioblastoma multiforme (GBM) is a highly aggressive brain tumor that is resistant to chemo- and radiotherapies. The reprogramming of lipid metabolism in tumor cells and its localization remains unclear. This study used ToF-SIMS to spatially probe lipid composition in different regions of a GBM tumor, revealing distinct distributions of lipid species.
ACS CHEMICAL NEUROSCIENCE
(2023)
Review
Chemistry, Multidisciplinary
Melanie A. Brunet, Mary L. Kraft
Summary: Characterizing the subcellular distributions of biomolecules is crucial for understanding their role in biological functions. However, imaging specific lipid species and cholesterol without perturbing their distributions is difficult. This challenge has been addressed by using rare stable isotopes as labels and the Cameca NanoSIMS 50 instrument for high-resolution imaging.
ACCOUNTS OF CHEMICAL RESEARCH
(2023)
Article
Acoustics
Sophinese Iskander-Rizk, Mirjam Visscher, Astrid M. Moerman, Suze-Anne Korteland, Kim Van der Heiden, Antonius F. W. Van der Steen, Gijs Van Soest
Summary: In this study, lipid photoacoustic spectral signatures of plaque were extracted from human endarterectomy samples using optical resolution photoacoustic microscopy, with correlation found mainly with sphingomyelins and cholesteryl esters. Variability in lipid spectroscopic features revealed three distinct components with specific peak wavelengths. These findings will aid in the development of PA-based staging of atherosclerosis by capitalizing on lipidomics of atherosclerotic tissue.
Article
Hematology
Erin H. Seeley, Zhipeng Liu, Shuai Yuan, Chad Stroope, Elizabeth Cockerham, Nabil A. Rashdan, Luisa F. Delgadillo, Alexandra C. Finney, Dhananjay Kumar, Sandeep Das, Babak Razani, Wanqing Liu, James Traylor, A. Wayne Orr, Oren Rom, Christopher B. Pattillo, Arif Yurdagul Jr
Summary: Researchers used mass spectrometry imaging to characterize the spatial distribution of metabolites in stable and unstable atherosclerosis. They found that pathways related to lipid metabolism and long-chain fatty acids were enriched in stable plaques, while reactive oxygen species, aromatic amino acid, and tryptophan metabolism were increased in unstable plaques. This study provides valuable insights into the metabolic pathways involved in plaque destabilization and opens new avenues of research in cardiovascular disease.
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2023)
Article
Biochemical Research Methods
Hongxia Bai, Keith E. Linder, David C. Muddiman
Summary: This study introduces the use of IR-MALDESI in ablation-based 3D MSI for lipid analysis in fresh frozen skin tissue, exploring depth resolution with different laser energy levels. A total of 594 lipids were detected in a 56-layer 3D imaging experiment, revealing detailed lipid profiles across different skin layers.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Xiaoyu Zhou, Zhuofan Wang, Jingjin Fan, Zheng Ouyang
Summary: Ion mobility is utilized in mass spectrometers for analyzing the structure of biomolecules. Researchers demonstrate that ion mobility analysis in an ion trap with ultra-high fields can achieve resolutions over 10,000 for isomer separation, as shown for disaccharide, phospholipid, and peptide isomers. Elucidating complex structures of biomolecules is crucial in chemistry and life sciences. In the last decade, ion mobility coupled with mass spectrometry has become a unique tool for distinguishing isomers and isoforms of biomolecules. This study presents a concept for ion mobility analysis using an ion trap, enabling ultra-high field isomer separation and achieving resolutions over 10,000. The potential of this technology is demonstrated for analyzing isomers of disaccharides, phospholipids, and peptides with post-translational modifications.
NATURE COMMUNICATIONS
(2023)
Article
Immunology
Roel Tans, Shoumit Dey, Nidhi Sharma Dey, Jian-Hua Cao, Prasanjit S. Paul, Grant Calder, Peter O'Toole, Paul M. Kaye, Ron M. A. Heeren
Summary: This study uses mass spectrometry imaging (MSI) to spatially map hepatic lipid composition following Leishmania donovani infection in mice. The results identify specific lipids that correlate to hepatic granulomas and show an increase in unique phosphatidylglycerols (PGs) in infected liver tissue. These findings provide insight into the pathogenesis of Leishmania infection and the regulation of inflammatory cascades.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Mai H. Philipsen, Elias Ranjbari, Chaoyi Gu, Andrew G. Ewing
Summary: The changes in brain lipids induced by Modafinil treatment in Drosophila melanogaster show a decrease in phosphatidylcholine and sphingomyelin species, while an increase in phosphatidylethanolamine and phosphatidylinositol levels, which is consistent with previous studies on cognition-related drugs. These alterations offer insight into the potential mechanism of action of Modafinil in the brain and provide a potential target for the treatment of certain disorders.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Medical Laboratory Technology
Long Zou, Luolin Wang, Lei Guo, Weixun Zhou, Zhizhen Lai, Cheng Zhu, Xi Wu, Zhili Li, Aiming Yang
Summary: This study found that regions associated with early gastric cancer have different lipid distributions and enzyme expressions compared to paracancerous regions. Phosphatidylcholine (32:0) and phosphatidylcholine (34:3) in serum are potential biomarkers for discriminating between early gastric cancer patients and healthy controls.
CLINICA CHIMICA ACTA
(2022)
Article
Biochemical Research Methods
Jonathan T. Specker, Boone M. Prentice
Summary: The diverse array of chemical compounds in tissue samples can result in many isobaric compounds in imaging mass spectrometry experiments. High-resolution accurate mass measurements can help resolve these compounds, but may not always be feasible. Alternatively, tandem mass spectrometry can be used to improve molecular specificity. Gas-phase ion/ion reactions can selectively react with desired analytes and differentiate isobaric compounds. This method has been used to differentiate isobaric lipids in the imaging mass spectrometry analysis of rat brain tissue.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2023)
Article
Materials Science, Multidisciplinary
Ruiyang Sun, Weiwei Tang, Bin Li
Summary: Surface-assisted laser desorption/ionization mass spectrometry imaging is a promising technique for reducing interference and ion suppression. In this study, gold nanoparticles modified TiO2 nanospheres were used to fabricate a high-performance SALDI substrate. Compared to traditional matrices, this substrate provided higher sensitivity, lower interference, dual-polarity detection, and enhanced ionization efficiency for various molecules, especially neutral lipids. SALDI MSI using this substrate could be performed on both animal and plant tissues, revealing the spatial distribution of different molecular species.
APPLIED MATERIALS TODAY
(2022)
Review
Biochemistry & Molecular Biology
Wendy H. Muller, Edwin De Pauw, Johann Far, Cedric Malherbe, Gauthier Eppe
Summary: Mass spectrometry imaging (MSI) is crucial for understanding the spatial distribution of lipid species in biological samples, and Surface-Assisted Laser Desorption/Ionization (SALDI) MSI offers unique capabilities for lipid analysis, especially for lipids with low ionization efficiency in MALDI-MS.
PROGRESS IN LIPID RESEARCH
(2021)
Article
Chemistry, Analytical
Jian Zhang, Qingce Zang, Wei Xu, Fei Tang
Summary: This paper proposes a imaging method based on photoepoxidation coupled with air-flow-assisted desorption electrospray ionization mass spectrometry (AFADESI-MS) to rapidly characterize unsaturated lipids at the isomer level. The method demonstrates fast response speed, high epoxide yield (>80%), and high diagnostic ion abundance. The experiment reveals that the ratio of unsaturated lipid isomers varies in different types of tumor samples.
Article
Chemistry, Analytical
Sanaz C. Habibi, Gabe Nagy
Summary: In recent years, ion mobility spectrometry-mass spectrometry (IMS-MS) has advanced omics-based research with high-resolution platforms, but most separations have been qualitative. The rotational averaged ion neutral collision cross section (CCS) is a quantitative metric for characterizing biomolecules in IMS-MS. Determining CCS in multipass IMS systems has been challenging due to a lack of methods for calculating CCS and the difficulty of requiring identical passes for calibrants and unknown compounds. This study presents a method using average ion velocities to calculate CCS values in cyclic IMS-MS separations, demonstrating good agreement with previous literature and potential applicability to other IMS-MS platforms.
ANALYTICAL CHEMISTRY
(2023)
Article
Ophthalmology
Hannah E. Bowrey, Alexandra P. Metse, Amelia J. Leotta, Guang Zeng, Sally A. McFadden
CLINICAL AND EXPERIMENTAL OPTOMETRY
(2015)
Editorial Material
Neurosciences
Hannah E. Bowrey, Morgan H. James
FRONTIERS IN CELLULAR NEUROSCIENCE
(2015)
Article
Neurosciences
Morgan H. James, Colin M. Stopper, Benjamin A. Zimmer, Nikki E. Koll, Hannah E. Bowrey, Gary Aston-Jones
BIOLOGICAL PSYCHIATRY
(2019)
Article
Neurosciences
Morgan H. James, Hannah E. Bowrey, Colin M. Stopper, Gary Aston-Jones
EUROPEAN JOURNAL OF NEUROSCIENCE
(2019)
Article
Ophthalmology
Amelia J. Leotta, Hannah E. Bowrey, Guang Zeng, Sally A. McFadden
OPHTHALMIC AND PHYSIOLOGICAL OPTICS
(2013)
Article
Multidisciplinary Sciences
Jie Gong, Mark A. Fields, Ernesto F. Moreira, Hannah E. Bowrey, Monika Gooz, Zsolt Ablonczy, Lucian V. Del Priore
Article
Multidisciplinary Sciences
Mark A. Fields, Hannah E. Bowrey, Jie Gong, Ernesto F. Moreira, Hui Cai, Lucian V. Del Priore
Article
Neurosciences
Guang Zeng, Hannah E. Bowrey, Jun Fang, Yanhua Qi, Sally A. McFadden
Article
Neurosciences
Aida Mohammadkhani, Jennifer E. Fragale, Caroline B. Pantazis, Hannah E. Bowrey, Morgan H. James, Gary Aston-Jones
JOURNAL OF NEUROSCIENCE
(2019)
Review
Psychology, Clinical
Hannah E. Bowrey, Morgan H. James, Gary Aston-Jones
DEPRESSION AND ANXIETY
(2017)
Article
Ophthalmology
Hannah E. Bowrey, Guang Zeng, Dennis Y. Tse, Amelia J. Leotta, Yi Wu, Chi-Ho To, Christine F. Wildsoet, Sally A. McFadden
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2017)
Article
Ophthalmology
Mark A. Fields, Hui Cai, Hannah E. Bowrey, Ernesto F. Moreira, Monika Beck Gooz, Kannan Kunchithapautham, Jie Gong, Emma Vought, Lucian V. Del Priore
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2015)
Article
Ophthalmology
Sally A. McFadden, Dennis Y. Tse, Hannah E. Bowrey, Amelia J. Leotta, Carly S. Lam, Christine F. Wildsoet, Chi-Ho To
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2014)