Journal
PROTEIN EXPRESSION AND PURIFICATION
Volume 120, Issue -, Pages 110-117Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.pep.2015.12.018
Keywords
Downstream processing; Purification of protein; Extraction of protein; Recombinant protein; Plant based platforms
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During the last two decades, the production of recombinant proteins in plant systems has been receiving increased attention. Currently, proteins are considered as the most important biopharmaceuticals. However, high costs and problems with scaling up the purification and isolation processes make the production of plant-based recombinant proteins a challenging task. This paper presents a summary of the information regarding the downstream processing in plant systems and provides a comprehensible overview of its key steps, such as extraction and purification. To highlight the recent progress, mainly new developments in the downstream technology have been chosen. Furthermore, besides most popular techniques, alternative methods have been described. (C) 2015 Elsevier Inc. All rights reserved.
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