Review
Biochemistry & Molecular Biology
Samuel Smalley, Hanjo Hellmann
Summary: This review provides an overview of how ubiquitin and similar proteins modify target proteins in plants to rapidly change their stability and activity, with a focus on their contribution to stress tolerance. The study also explores the potential use of these pathways for generating more robust crop plants to cope with stress situations.
Review
Plant Sciences
Shumei Fang, Xue Hou, Xilong Liang
Summary: SUMOylation is a crucial posttranslational modification in eukaryotes, involved in metabolic regulation under various stresses. SIZ1, a SUMO E3 ligase, plays a vital role in substrate-specific recognition and facilitates the binding of SUMO proteins to target proteins. This review provides insights into the characteristics of SUMOylation and the role of SIZ1 in plant adaptation to abiotic stresses, offering valuable references for crop improvement and future research on plant responses to environmental stress.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2022)
Article
Multidisciplinary Sciences
Stephanie L. Sarbanes, Vincent A. Blomen, Eric Lam, Soren Heissel, Joseph M. Luna, Thijn R. Brummelkamp, Erik Falck-Pedersen, H-Heinrich Hoffmann, Charles M. Rice
Summary: The journey of DNA viruses from the plasma membrane to the nuclear pore is crucial for viral replication, with MIB1 identified as a critical host factor for Adenovirus infectivity. MIB1's ubiquitination activity is essential for promoting AdV infection by potentially degrading negative regulators. This study highlights a new way that viruses exploit host cell machinery for replication, suggesting a potential target for therapeutic interventions against AdV infection.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Medicine, General & Internal
Zhonghui Zhang, Liang Zhong, Wenran Dan, Xuan Chu, Chen Liu, Xu Luo, Peng Wan, Zhenyan Liu, Yang Lu, Xiao Wang, Beizhong Liu
Summary: The research reveals that ZFP91 enhances AML cell proliferation and inhibits apoptosis by interacting with RIP1 and inhibiting its K48-linked ubiquitination. These findings provide evidence that targeted inhibition of ZFP91 could be a potential approach to treat AML.
INTERNATIONAL JOURNAL OF MEDICAL SCIENCES
(2022)
Review
Pharmacology & Pharmacy
Si-Min Qi, Jinyun Dong, Zhi-Yuan Xu, Xiang-Dong Cheng, Wei-Dong Zhang, Jiang-Jiang Qin
Summary: PROTAC technology is an effective method for degrading target proteins through the ubiquitin-proteasome system, with promising applications in cancer treatment. The first oral PROTACs have shown encouraging results in clinical trials, sparking greater enthusiasm for PROTAC research.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Ting Xu, Weiwei Yu, Hui Fang, Zhen Wang, Zhexu Chi, Xingchen Guo, Danlu Jiang, Kailian Zhang, Sheng Chen, Mobai Li, Yuxian Guo, Jian Zhang, Dehang Yang, Qianzhou Yu, Di Wang, Xue Zhang
Summary: The membrane-bound E3 ubiquitin ligase gp78 mediates mixed ubiquitination of NLRP3, restraining inflammasome activation by inhibiting NLRP3 oligomerization and subcellular translocation. Insig-1, an endoplasmic reticulum membrane protein, is required for the gp78-NLRP3 interaction and gp78-mediated NLRP3 ubiquitination. Deficiency of gp78 or Insig-1 in myeloid cells leads to exacerbated NLRP3 inflammasome-dependent inflammation in vivo.
CELL DEATH AND DIFFERENTIATION
(2022)
Article
Chemistry, Medicinal
Nobumichi Ohoka, Masanori Suzuki, Takuya Uchida, Genichiro Tsuji, Yoshinori Tsukumo, Masayuki Yoshida, Takao Inoue, Yosuke Demizu, Hitoshi Ohki, Mikihiko Naito
Summary: In this study, novel FLT3 degraders were developed by introducing an FDA-approved FLT3 inhibitor, gilteritinib, into targeted protein degradation technology. The most active compound, CRBN(FLT3)-8, potently degraded FLT3-ITD via the ubiquitin-proteasome system and effectively inhibited the proliferation of AML cells.
ACS MEDICINAL CHEMISTRY LETTERS
(2022)
Article
Cell Biology
Sujuan Gao, Xueqin Zeng, Jianhao Wang, Yingchao Xu, Chunwei Yu, Yishui Huang, Feng Wang, Keqiang Wu, Songguang Yang
Summary: The study reveals that the Arabidopsis SUMO E3 ligase SIZ1 interacts with HDA6 and negatively regulates the function of HDA6 by affecting the acetylation level of histone H3 and the binding of HDA6 to its target genes.
Review
Cell Biology
Emanuela Senatore, Rosa Iannucci, Francesco Chiuso, Rossella Delle Donne, Laura Rinaldi, Antonio Feliciello
Summary: Primary cilia are microtubule-based sensory organelles that receive and transmit external signals to control cell growth, differentiation, and development. The formation and disassembly of primary cilia are finely regulated during the cell cycle. The ubiquitin-proteasome system and autophagy machinery play essential roles in cilia dynamics.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Na Yang, Bo Kong, Zhaohong Zhu, Fei Huang, Liliang Zhang, Tao Lu, Yadong Chen, Yanmin Zhang, Yulei Jiang
Summary: Targeted protein degradation (TPD) technology, such as PROTACs, has become widespread in the past 20 years and greatly accelerates the development of disease treatment. Unlike small inhibitors, targeted protein degraders can target undruggable targets and overcome drug resistance through ubiquitin-proteasome pathway (UPP) and lysosome pathway. This review discusses different degradation technologies and provides a basis for future research.
MOLECULAR DIVERSITY
(2023)
Article
Biochemistry & Molecular Biology
Tommaso Palomba, Massimo Baroni, Simon Cross, Gabriele Cruciani, Lydia Siragusa
Summary: Proteolysis-targeting chimeras (PROTACs) are novel therapeutics that utilize E3 ligases to degrade target proteins. Existing databases on E3 ligases have limited information on their structures and ligands. This study presents an accurate and comprehensive platform, ELIOT, to navigate and select new E3 ligases and ligands for the design of new PROTACs.
CHEMICAL BIOLOGY & DRUG DESIGN
(2023)
Article
Virology
Behdokht Jan Fada, Udayan Guha, Yi Zheng, Eleazar Reward, Elie Kaadi, Ayette Dourra, Haidong Gu
Summary: It was found that ICP0 E3 enzyme continuously degrades PML I through a specific mechanism to prevent the reformation of ND10 complex.
Article
Biochemistry & Molecular Biology
Jiawei Xiong, Fabin Yang, Fan Wei, Feng Yang, Honghui Lin, Dawei Zhang
Summary: HLS1 plays a key role in the development of the apical hook in plants, and SIZ1 regulates the rapid opening of the apical hook by mediating HLS1 SUMOylation. The opening of the apical hook is associated with a decrease in SIZ1 transcript levels, leading to reduced HLS1 SUMOylation. In addition, HY5 promotes the rapid opening of the apical hook by inhibiting SIZ1 transcription.
Article
Oncology
Moyang Lv, Weichao Hu, Shengwei Zhang, Lijiao He, Changjiang Hu
Summary: Proteolysis-targeting chimeras (PROTACs) are a promising class of drugs that can effectively target undruggable proteins and have potential applications in cancer therapy. This review provides an overview of PROTAC technology and highlights recent clinical trials, as well as novel findings and strategies to enhance PROTAC antitumor activity.
Article
Fisheries
Yan Gao, Yu-Hang Xiang, Chen Li, Jing Ye, Yuan-An Lu, Usama Ashraf, Xue-Qin Liu
Summary: The study demonstrates that TRIM33 enhances SVCV replication by interacting with and degrading Viperin_sv1 protein. Additionally, SVCV infection increases TRIM33 expression, reducing interferon response and promoting viral replication.