Review
Cell Biology
Noritaka Tanaka, Takeharu Sakamoto
Summary: MT1-MMP is a member of the metalloproteinase family, which degrades extracellular matrix components directly and can indirectly degrade type IV collagen through the activation of MMP-2 with TIMP-2. MT1-MMP is initially expressed as an inactive form in the ER and is activated through truncation processing by furin. Upon trafficking from the ER to the cell surface membrane, MT1-MMP exhibits proteolytic activities on surrounding molecules.
Review
Cell Biology
Maike Kuemper, Joy Steinkamp, Paola Zigrino
Summary: This review focuses on the regulation of metalloproteinases in maintaining skin homeostasis, particularly in dermal homeostasis. It discusses their activity and expression regulation mechanisms and how the dysregulation of these metalloproteinases affects disease development, particularly cancer and aging. Furthermore, it emphasizes recent discoveries and new therapeutic approaches that target metalloproteinase activity, as well as the potential of naturally derived components in regulating metalloproteinase expression and activity in disease.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2022)
Article
Reproductive Biology
Yunxiang Li, Yuguang Chen, Wenping Wu, Na Li, Jinlian Hua
Summary: Metalloproteinases, including matrix metalloproteinases and disintegrin metalloproteinases, play important roles in the degradation and reconstruction of the extracellular matrix (ECM). They are associated with tissue development and disease. Although their relationship with male reproduction is clear, the underlying mechanism is still unclear.
Review
Biochemistry & Molecular Biology
Il-Sup Kim, Woong-Suk Yang, Cheorl-Ho Kim
Summary: MMPs are enzymes that play a key role in promoting cell migration by degrading the extracellular matrix (ECM). They are upregulated in cancers and inflamed regions. TIMPs are highly specific inhibitors of MMPs, regulating their activity and potentially playing a role in inhibiting MMP-dependent diseases.
CURRENT MEDICINAL CHEMISTRY
(2023)
Article
Ecology
Nour Al Haj Baddar, Nataliya Timoshevskaya, Jeramiah J. Smith, Houfu Guo, S. Randal Voss
Summary: This study identified 28 MMPs in salamanders, with 9 MMP paralogs unique to axolotls and other salamanders. These novel salamander MMPs are all of the secreted type, suggesting a unique expansion within salamanders.
FRONTIERS IN ECOLOGY AND EVOLUTION
(2021)
Article
Chemistry, Medicinal
Defeng Xu, Caihong Li, Mouming Zhao
Summary: The extract from Theragra chalcogramma (TCH) can improve the barrier functions of photoaged skin, promote the deposition of extracellular matrix (ECM) components, and inhibit the activity of MMP-1 in photoaging skin. It also restores the impaired structures in the skin and enhances the expression levels of elastin and fibrillin-1.
Article
Food Science & Technology
Maha Sayari, Houcine Dab, Mounira Tlili, Lazhar Zourgui, Abdelmajid Khabir
Summary: This study aimed to investigate the impact of yellow lupin extract (YLE) on renal and hepatic extracellular matrix (ECM) in diabetes. The results showed that diabetes increased the contents of collagen, laminin, and fibronectin in the liver and kidney. YLE treatment partially improved the tissue disorders caused by diabetes.
FOOD SCIENCE & NUTRITION
(2023)
Article
Biochemistry & Molecular Biology
Ludwig Werny, Antonia Grogro, Kira Bickenbach, Cynthia Bulck, Fred Armbrust, Tomas Koudelka, Kriti Pathak, Franka Scharfenberg, Martin Sammel, Farah Sheikhouny, Andreas Tholey, Stefan Linder, Christoph Becker-Pauly
Summary: This study investigated the interaction between membrane-type-I matrix metalloproteinase (MT1-MMP) and meprin beta, revealing a potential regulatory connection between the two enzymes.
Article
Pharmacology & Pharmacy
Ming Zhang, Rui Zhang, Tiansheng Zheng, Zhixi Chen, Guanglin Ji, Fang Peng, Wei Wang
Summary: Xanthohumol (XH) attenuates inflammatory responses and extracellular matrix (ECM) degradation in osteoarthritis chondrocytes by mediating HO-1 and C/EBPβ signaling pathways.
FRONTIERS IN PHARMACOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Hyun Lee, Lucas Ibrahimi, Dimitri T. Azar, Kyu-Yeon Han
Summary: MT1-MMP is a protease enzyme that regulates cellular activities, especially cell migration. It interacts with various molecules and can not only change the extracellular environment but also regulate cell signaling.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Oncology
Rajalakshmi Sabanayagam, Sneha Krishnamoorthy, Jayapradha Gnanagurusamy, Bharathi Muruganatham, Sridhar Muthusami
Summary: This study aimed to investigate the effects of epigallocatechin gallate (EGCG) in preventing the actions of epidermal growth factor (EGF) in HPV positive and HPV negative cervical cancer cells. The results showed that EGCG pre-treatment blocked EGF-induced colony formation and prevented epithelial-mesenchymal transition (EMT). EGCG has the potential to act as a supplementary therapy for EGF-driven cervical cancer.
Article
Surgery
A. Winsnes, M-L Ivarsson, P. Falk, U. Gunnarsson, K. Strigard
Summary: This study evaluated the tissue remodelling of autologous full-thickness skin grafting (FTSG) in the intraperitoneal and onlay positions in mice. The results suggest that both positions are promising options for abdominal wall repair.
Article
Cell Biology
Miriam Bobadilla Munoz, Josune Orbe, Gloria Abizanda, Florencio J. D. Machado, Amaia Vilas, Asier Ullate-Agote, Leire Extramiana, Arantxa Baraibar Churio, Xabier L. Aranguren, Gloria Cantero, Neira Sainz Amillo, Jose Antonio Rodriguez, Luis Ramos Garcia, Juan Pablo Romero Riojas, Ainara Vallejo-Illarramendi, Carmen Paradas, Adolfo Lopez de Munain, Jose Antonio Paramo, Felipe Prosper, Ana Perez-Ruiz
Summary: Aged muscles have a decline in their response to damage, which is mainly attributed to intrinsic defects in satellite cells. However, changes in the muscle-stem cell microenvironment also contribute to aging. Loss of MMP-10 in young mice alters the muscle ECM and disrupts the satellite cell niche, leading to premature aging features. MMP-10 has a protective effect on satellite cells and can delay their aging and overcome dysfunction in muscular dystrophy.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Dentistry, Oral Surgery & Medicine
Igor Paulino Mendes Soares, Caroline Anselmi, Isabela Guine, Lidia de Oliveira Fernandes, Maria Luiza Barucci Araujo Pires, Carlos Alberto de Souza Costa, Debora Lopes Salles Scheffel, Josimeri Hebling
Summary: The ion-releasing filler (S-PRG) eluate showed inhibitory activity on dentin collagen-bound metalloproteinases (MMPs) and dentin matrix degradation. Short-term treatment with S-PRG eluate may help prevent tooth decay and dentin degradation.
JOURNAL OF DENTISTRY
(2022)
Article
Dentistry, Oral Surgery & Medicine
Igor Paulino Mendes Soares, Caroline Anselmi, Isabela Guine, Lidia de Oliveira Fernandes, Maria Luiza Barucci Araujo Pires, Carlos Alberto de Souza Costa, Debora Lopes Salles Scheffel, Josimeri Hebling
Summary: This study evaluated the inhibitory activity of an ion-releasing filler on dentin collagen-bound metalloproteinases and dentin matrix degradation. The results showed that the ion-releasing filler reduced metalloproteinase activity and dentin degradation, potentially enhancing the stabilization of dentin bonding interfaces.
JOURNAL OF DENTISTRY
(2022)
Article
Engineering, Biomedical
Keren Kahil, Ifat Kaplan-Ashiri, Sharon G. Wolf, Katya Rechav, Steve Weiner, Lia Addadi
Summary: During spicule formation in sea urchin larvae, calcium ions are concentrated and precipitated while other abundant ions are removed. Analytical techniques such as cryo-SEM-EDS and cryo-STEM-EDS are used to study the overall ion composition in vesicles and their potential applicability in studying ion transport pathways.
ACTA BIOMATERIALIA
(2023)
Correction
Cell & Tissue Engineering
Berengere de Laval, Julien Maurizio, Prashanth K. Kandalla, Gabriel Brisou, Louise Simonnet, Caroline Huber, Gregory Gimenez, Orit Matcovitch-Natan, Susanne Reinhardt, Eyal David, Alexander Mildner, Achim Leutz, Bertrand Nadel, Christophe Bordi, Ido Amit, Sandrine Sarrazin, Michael H. Sieweke
Article
Hematology
Avigail Rein, Ifat Geron, Eitan Kugler, Hila Fishman, Eyal Gottlieb, Ifat Abramovich, Amir Giladi, Ido Amit, Roger Mulet-Lazaro, Ruud Delwel, Stefan Groeschel, Smadar Levin-Zaidman, Nili Dezorella, Vered Holdengreber, Tata Nageswara Rao, Joanne Yacobovich, Orna Steinberg-Shemer, Qiu-Hua Huang, Yun Tan, Sai-Juan Chen, Shai Izraeli, Yehudit Birger
Summary: Mono-allelic germline disruptions of the GATA2 transcription factor can lead to myelodysplastic syndrome (MDS) and acute myeloid leukemia (AML). This study shows the role of GATA2 mutations in the development of AML and identifies mitochondrial abnormalities as a potential therapeutic target for preventing leukemic transformation.
Article
Immunology
Saskia Rauber, Christina B. Schroeter, Christine Strippel, Christopher Nelke, Tillmann Ruland, Andre Dik, Kristin S. Golombeck, Liesa Regner-Nelke, Manuela Paunovic, Daniela Esser, Christian Muench, Felix Rosenow, Martijn van Duijn, Antonia Henes, Tobias Ruck, Ido Amit, Frank Leypoldt, Maarten J. Titulaer, Heinz Wiendl, Sven G. Meuth, Gerd Meyer zu Horste, Nico Melzer
Summary: Autoimmune encephalitis (AE) is a group of non-infectious inflammatory conditions of the central nervous system caused by an imbalanced immune response. Analysis of cerebrospinal fluid (CSF) protein profiles in AE patients with different autoantibodies revealed dysregulation in the complement system, imbalance between pro-inflammatory and anti-inflammatory proteins, dysregulation of proteins involved in synaptic transmission and neurodegeneration, as well as elevated levels of proteases and reduced protease inhibitors. Furthermore, distinct protein profiles were found in different AE subtypes, which may aid in identifying disease-specific biomarkers.
JOURNAL OF AUTOIMMUNITY
(2023)
Correction
Neurosciences
Mor Kenigsbuch, Pierre Bost, Shahar Halevi, Yuzhou Chang, Shuo Chen, Qin Ma, Renana Hajbi, Benno Schwikowski, Bernd Bodenmiller, Hongjun Fu, Michal Schwartz, Ido Amit
NATURE NEUROSCIENCE
(2023)
Article
Multidisciplinary Sciences
Junil Kim, Michaela Mrugala Rothova, Esha Madan, Siyeon Rhee, Guangzheng Weng, Antonio M. Palma, Linbu Liao, Eyal David, Ido Amit, Morteza Chalabi Hajkarim, Vignesh Vudatha, Andres Gutierrez-Garcia, Eduardo Moreno, Robert Winn, Jose Trevino, Paul B. Fisher, Joshua M. Brickman, Rajan Gogna, Kyoung Jae Won
Summary: Development of multicellular organisms relies on persistent cell-cell communication, which drives lineage specification and cell fate decisions. The current single-cell RNA-seq technology lacks spatial information needed to analyze cell-cell contact-dependent gene expression. To overcome this, we performed RNA sequencing of physically interacting cells (PIC-seq) and compared them with single-cell transcriptomes from developing mouse embryos. Our analysis identified cell-type-dependent gene expression signatures, and we developed a computational model to predict the impact of one cell type on neighboring cell types. We also developed a spatial embedding method to visualize the distribution of cells in a 2D space. This innovative approach allows us to study contact-specific gene regulation during embryogenesis.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Multidisciplinary Sciences
Jennifer Cable, Jeffrey C. Rathmell, Erika L. Pearce, Ping-Chih Ho, Marcia C. Haigis, Murad R. Mamedov, Meng-Ju Wu, Susan M. Kaech, Lydia Lynch, Mark A. Febbraio, Sagar P. Bapat, Hanna S. Hong, Weiping Zou, Yasmine Belkaid, Zuri A. Sullivan, Andrea Keller, Stefanie K. Wculek, Douglas R. Green, Catherine Postic, Ido Amit, Salvador Aznar Benitah, Russell G. Jones, Miguel Reina-Campos, Santiago Valle Torres, Semir Beyaz, Donal Brennan, Luke A. J. O'Neill, Rachel J. Perry, Dirk Brenner
Summary: Immunometabolism studies the relationship between metabolism and immunity, with a focus on the metabolic pathways within immune cells and the impact of immune cells on systemic metabolism. In the Keystone symposium on Immunometabolism at the Crossroads of Obesity and Cancer, experts presented recent research on the complex interplay between metabolism, immunity, and cancer, highlighting metabolic links between tumor cells and immune cells, the effects of diet, the microbiome, and obesity on immune system function and cancer, and new technologies to study the immune system and uncover novel metabolic pathways.
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES
(2023)
Article
Cell Biology
Svetlana Markman, Mor Zada, Eyal David, Amir Giladi, Ido Amit, Elazar Zelzer
Summary: Through single-cell RNA sequencing of developing mouse forelimb, we identify three progenitor states: naive, proximal, and autopodial, with Msx1 as a marker for naive progenitors. Lineage tracing confirms the role of naive progenitors and locates them at the outer margin of the limb along the anterior-posterior axis. Sequential pulse-chase experiments show that the progressive transition of Msx1+ naive progenitors into proximal and autopodial progenitors coincides with their differentiation into Sox9+ chondroprogenitors, occurring along all the forming skeletal segments. Hence, the skeleton forms progressively in a complex pattern.
DEVELOPMENTAL CELL
(2023)
Article
Biochemistry & Molecular Biology
Peter Kirchweger, Debakshi Mullick, Prabhu Prasad Swain, Sharon G. Wolf, Michael Elbaum
Summary: Visualization of organelles and their interactions with other features in the native cell remains a challenge in modern biology. We introduced cryo-scanning transmission electron tomography (CSTET) which enables high-resolution 3D imaging on a micron scale, and demonstrated the utility of cryo-SRRF nanoscopy and deconvolution processing in enhancing precision and resolution. The integration of cryo-SRRF with deconvolved dual-axis CSTET provides a versatile workflow for studying unique objects in a cell.
JOURNAL OF STRUCTURAL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Bjort K. Kragesteen, Amir Giladi, Eyal David, Shahar Halevi, Laufey Geirsdottir, Olga M. Lempke, Baoguo Li, Andreas M. Bapst, Ken Xie, Yonatan Katzenelenbogen, Sophie L. Dahl, Fadi Sheban, Anna Gurevich-Shapiro, Mor Zada, Truong San Phan, Roberto Avellino, Shuang-Yin Wang, Oren Barboy, Shir Shlomi-Loubaton, Sandra Winning, Philipp P. Markwerth, Snir Dekalo, Hadas Keren-Shaul, Merav Kedmi, Martin Sikora, Joachim Fandrey, Thorfinn S. Korneliussen, Josef T. Prchal, Barak Rosenzweig, Vladimir Yutkin, Fernando Racimo, Eske Willerslev, Chamutal Gur, Roland H. Wenger, Ido Amit
Summary: Single-cell RNA and transposase-accessible chromatin (ATAC) sequencing in a mouse model identified a rare cell subset called Norn cells in kidney stroma as the major source of endocrine Epo production in mice, and this finding was confirmed in human kidney tissues. These findings provide new insights into EPO gene regulation and may lead to improved therapies for anemia.
Article
Cell Biology
Thomas Look, Emanuele Puca, Marcel Buhler, Daniel Kirschenbaum, Roberto De Luca, Riccardo Stucchi, Domenico Ravazza, Cesare Di Nitto, Patrick Roth, Yonatan Katzenelenbogen, Assaf Weiner, Lukas Rindlisbacher, Burkhard Becher, Ido Amit, Michael Weller, Dario Neri, Teresa Hemmerle, Tobias Weiss
Summary: In this study, a combination therapy of L19TNF, an antibody-cytokine fusion protein, and CCNU, an alkylating agent, demonstrated strong anti-glioma activity in mouse models. The combination treatment induced tumor DNA damage, tumor necrosis, up-regulation of tumor endothelial cell adhesion molecules, immune cell infiltration, immunostimulatory pathways, and decreased immunosuppression pathways. Clinical translation of this combination therapy in glioblastoma patients has shown objective responses in a patient cohort, indicating its potential efficacy.
SCIENCE TRANSLATIONAL MEDICINE
(2023)
Review
Oncology
Oren Barboy, Yonatan Katzenelenbogen, Rotem Shalita, Ido Amit
Summary: CAR T-cell therapies have the potential to revolutionize cancer treatment, but face challenges in solid tumor settings. Understanding their mechanism of action and clinical implications is crucial. Combining single-cell genomics and cell engineering tools can accelerate CAR T-cell development.
Article
Oncology
Omer Goldman, Lital N. Adler, Emma Hajaj, Tommaso Croese, Naama Darzi, Sivan Galai, Hila Tishler, Yarden Ariav, Dor Lavie, Liat Fellus-Alyagor, Roni Oren, Yuri Kuznetsov, Eyal David, Rami Jaschek, Chani Stossel, Oded Singer, Sergey Malitsky, Renana Barak, Rony Seger, Neta Erez, Ido Amit, Amos Tanay, Ann Saada, Talia Golan, Tamar Rubinek, Joo Sang Lee, Shay Ben-Shachar, Ido Wolf, Ayelet Erez
Summary: Multiple studies have found metabolic changes within the tumor and its micro-environment during carcinogenesis, but the mechanisms by which tumors affect the host metabolism are unclear. This study discovered that systemic inflammation induced by cancer results in the infiltration of myeloid cells into the liver, leading to the depletion of a master metabolic regulator, HNF4α, and subsequently causing systemic metabolic changes that promote breast and pancreatic cancer proliferation.
Article
Multidisciplinary Sciences
Peter Kirchweger, Debakshi Mullick, Sharon Grayer Wolf, Michael Elbaum
Summary: Cryo-EM is a method of imaging biological or organic specimens in their native aqueous medium, allowing for the determination of their structure at near-atomic resolution. This method has been extended to the study of organelles and cells using tomography, overcoming the thickness limitation of traditional transmission EM imaging. This article presents a setup for cryo-tomography using STEM, enabling the detailed visualization of organelles in the cytoplasm.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2023)
Article
Microbiology
Andre G. Gouveia, Bruno A. Salgueiro, Dean O. Ranmar, Wilson D. T. Antunes, Peter Kirchweger, Ofra Golani, Sharon G. Wolf, Michael Elbaum, Pedro M. Matias, Celia V. Romao
Summary: Arsenic is a toxic heavy metal that is widely present in the environment and can severely harm water resources. Bioremediation is a sustainable and cost-effective technology for removing toxic compounds. The resistance of Deinococcus indicus, an arsenic resistant bacterium, to cellular insults was extensively characterized to explore its potential use in arsenic bioremediation.
FRONTIERS IN MICROBIOLOGY
(2023)