Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 113, Issue 32, Pages 9003-9008Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1602716113
Keywords
beta-barrel pores; nanopore-based sensing; polymer confinement; polymer transport; macromolecular crowding
Categories
Funding
- US Department of Energy, Office of Basic Energy Sciences, Division of Materials Sciences and Engineering Award [DE-SC0008176]
- Intramural Research Program of the NIH, Eunice Kennedy Shriver National Institute of Child Health and Human Development
- National Science Foundation EAGER Award [1249199]
- National Science Foundation [DMR-1504265]
- Air Force Office of Scientific Research Grant [FA9550-14-1-0164]
- NIH [R01HG002776-11]
- Div Of Chem, Bioeng, Env, & Transp Sys
- Directorate For Engineering [1249199] Funding Source: National Science Foundation
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Nonideal polymer mixtures of PEGs of different molecular weights partition differently into nanosize protein channels. Here, we assess the validity of the recently proposed theoretical approach of forced partitioning for three structurally different beta-barrel channels: voltage-dependent anion channel from outer mitochondrial membrane VDAC, bacterial porin OmpC (outer membrane protein C), and bacterial channel-forming toxin alpha-hemolysin. Our interpretation is based on the idea that relatively less-penetrating polymers push the more easily penetrating ones into nanosize channels in excess of their bath concentration. Comparison of the theory with experiments is excellent for VDAC. Polymer partitioning data for the other two channels are consistent with theory if additional assumptions regarding the energy penalty of pore penetration are included. The obtained results demonstrate that the general concept of polymers pushing polymers is helpful in understanding and quantification of concrete examples of size-dependent forced partitioning of polymers into protein nanopores.
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