Article
Biochemistry & Molecular Biology
Chiara Greggi, Ida Cariati, Federica Onorato, Riccardo Iundusi, Manuel Scimeca, Umberto Tarantino
Summary: The study revealed that PTX3 plays a role in osteogenic differentiation and possesses osteoinductive capacity, making it a potential biomarker and therapeutic agent for osteoporosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Nanoscience & Nanotechnology
Hong Wang, Yingzhen Lai, Zeyu Xie, Yanyin Lin, Yihuang Cai, Zhiqiang Xu, Jiang Chen
Summary: Surface modification of titanium implants is an effective method to improve their biocompatibility and mechanical properties. In this study, a concentric microgrooved titanium surface with osteon-mimetic features was constructed and further modified with graphene oxide. The modified surface showed excellent biocompatibility and promoted the proliferation of bone marrow-derived mesenchymal stem cells and macrophages. The microgrooves on the titanium surface guided cell migration and altered cell morphology. Moreover, the GO-modified surface promoted osteogenic differentiation and inhibited osteoclastogenic differentiation. Using an indirect coculture system, it was found that the modified surface regulated the secretion of specific factors and influenced cell differentiation. The dual effects of osteogenesis and antiosteoclastogenesis make this surface modification technique promising for implant osseointegration.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Dentistry, Oral Surgery & Medicine
Manuel Toledano-Osorio, Elvira de Luna-Bertos, Manuel Toledano, Francisco Javier Manzano-Moreno, Victor Costela-Ruiz, Concepcion Ruiz, Javier Gil, Raquel Osorio
Summary: This study evaluated the effect of doxycycline and dexamethasone doped nanoparticles on osteoblasts proliferation and differentiation when applied on titanium surfaces. The results showed that these nanoparticles promoted osteoblasts differentiation, up-regulated the expression of osteogenic genes, and altered cell morphology. Therefore, using these nanoparticles in regenerative procedures around titanium dental implants could potentially create a favorable osteogenic environment.
Review
Polymer Science
Aiah A. El-Rashidy, Sara El Moshy, Israa Ahmed Radwan, Dina Rady, Marwa M. S. Abbass, Christof E. Doerfer, Karim M. Fawzy El-Sayed
Summary: The stiffness of the extracellular matrix plays a regulatory role in directing the osteogenic differentiation of MSCs, with MSCs sensing and responding to their ECM to undergo differentiation. Customizing scaffold materials to mimic cells' natural environment is crucial in the tissue engineering process.
Article
Anatomy & Morphology
Celio Junior da Costa Fernandes, Ana Flavia Balestrero Cassiano, Flavio Henrique-Silva, Joni Augusto Cirelli, Eduardo Pereira de Souza, Hernan Coaguila-Llerena, Willian Fernando Zambuzzi, Gisele Faria
Summary: Cysteine protease inhibitors, specifically sugarcane-derived cystatin (CaneCPI-5), were shown to negatively modulate adhesion and cytoskeleton remodeling, while also promoting cell migration and matrix metalloproteinase activation in osteoblasts. This study suggests that CaneCPI-5 can trigger mechanisms related to osteoblast differentiation, providing new perspectives for biotechnological approaches to bone disorders.
Article
Biochemistry & Molecular Biology
Li Peng, Fanzi Wu, Mengjiao Cao, Mengxin Li, Jingyao Cui, Lijia Liu, Yun Zhao, Jing Yang
Summary: Osteoblasts are key players in bone formation and can sense mechanical stimuli, converting them into biochemical responses through mechanical transduction. These cells actively participate in osteogenesis and the formation and mineralization of the extracellular bone matrix. This review summarizes the physiological and biological mechanisms behind five different physical stimuli (light, electricity, magnetism, force, and sound) that induce osteogenesis, and also discusses the effects of culture conditions on the morphology, structure, and function of osteoblasts. These findings provide a theoretical foundation for studying bone physiology and pathology at the cellular level and have implications in the clinical application of bone formation and regeneration technology.
Article
Chemistry, Multidisciplinary
Georgia-Ioanna Kontogianni, Catarina Coelho, Remy Gauthier, Sonia Fiorilli, Paulo Quadros, Chiara Vitale-Brovarone, Maria Chatzinikolaidou
Summary: Nanohydroxyapatite (nanoHA) is a highly biocompatible and osteoconductive material for bone regeneration. By enriching nanoHA with strontium ions, enhanced mechanical properties and biological activity can be achieved. In this study, nanoHA and nanoHA with different substitution degrees of calcium with strontium ions (Sr-nanoHA_50 and Sr-nanoHA_100) were evaluated for their cytotoxicity and osteogenic potential. All three nanoHA-based materials showed cytocompatibility, needle-shaped nanocrystals, and enhanced osteogenic activity. Gene expression analysis demonstrated significant upregulation of osteocalcin, osteonectin, and osteopontin in all three compositions compared to the control. These findings indicate the great potential of the produced compounds for bone regeneration.
Article
Materials Science, Multidisciplinary
Qingchun Zheng, Lulu Mao, Yutao Shi, Weihua Fu, Yahui Hu
Summary: In this paper, laser processing technology was used to create microgrooves on the surface of Ti-6Al-4V titanium alloy implants. The effects of these microgrooves on cell behavior were studied, and it was found that they promoted cell proliferation and differentiation by increasing wettability and surface roughness.
MATERIALS TECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Paulina Kazimierczak, Malgorzata Koziol, Agata Przekora
Summary: The newly developed biomaterial showed high biocompatibility and influenced macrophage polarization and osteoblast differentiation, playing an immunomodulatory role. The study demonstrated a low risk of inflammatory response and induced macrophage polarization into the osteogenic phenotype M2, making it a promising bone scaffold for regenerative medicine applications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Huike Ma, Tingting Gao, Liu Wang, Ali Mohsin, Jie Hao, Meijin Guo, Jun Wu
Summary: Bone defects, a clinically refractory orthopaedic disease, currently have no effective treatments. Mesenchymal stem cells (MSCs) can differentiate into osteoblasts and be used as potential seed cells for bone tissue engineering. However, the feasibility of using MSCs for bone tissue engineering and the preparation of large-scale cell-scaffold remain unresolved.
CELL PROLIFERATION
(2023)
Article
Chemistry, Multidisciplinary
Huichang Gao, Jin Xiao, Yingqi Wei, Hao Wang, Hongxia Wan, Shan Liu
Summary: "Inspired by the natural topological structure of skeletal muscle tissue, bionic scaffolds with various microgroove topographies were fabricated and studied for their effects on skeletal muscle cell behaviors, especially myogenic differentiation. The results showed that the width of microgroove structures had a stronger effect on myogenic differentiation compared to their depth, with wider grooves enhancing myoblast differentiation. The integrin-mediated focal adhesion kinase signaling pathway and MAPK signaling pathway were activated in response to the external topological structure, indicating a potential mechanism for regulating cellular response in skeletal muscle tissue engineering."
Article
Engineering, Biomedical
Ho-Ying Wan, Rita Lih Ying Shin, Jack Chun Hin Chen, Marisa Assuncao, Dan Wang, Susie K. Nilsson, Rocky S. Tuan, Anna Blocki
Summary: The study demonstrated that supplementation of Dextran sulfate (DxS) in osteogenically induced cultures of human mesenchymal stem cells (MSCs) amplified the deposition of osteoblastic extracellular matrix (ECM). This enhancement led to increased collagen type I deposition and osteogenic gene expression, as well as enrichment in bone morphogenetic protein 2 (BMP-2), suggesting a potential role of DxS in promoting osteogenic differentiation by MSCs.
ACTA BIOMATERIALIA
(2022)
Article
Nanoscience & Nanotechnology
Weimin Fu, Xiuzhi Zhang, Benjie Wang, Hui Xie, Nannan Jiang, Shun Han, Ke Zhou, Yingjie Ma, Tianshuang Qiu, Dewei Zhao
Summary: Zinc nanoparticles are commonly used in biomedical applications, but their role in the osteogenic differentiation of mouse bone marrow stromal cells (BMSCs) is not fully understood. This study investigated the effects of zinc on the adipogenic and osteogenic differentiation of BMSCs via the TRPM7 pathway. The results suggested that zinc may protect bone through modulating the differentiation of BMSCs away from adipocytes.
JOURNAL OF BIOMEDICAL NANOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Anne M. Skelton, D. Joshua Cohen, Barbara D. Boyan, Zvi Schwartz
Summary: This study aimed to investigate the characteristics of osteoblast-derived matrix vesicles (MV) in the regulation of extracellular vesicles and osteoblast differentiation, and to assess their mechanism of uptake. The results showed that MVs exhibited increased alkaline phosphatase activity, specific size and membrane protein markers, and played a regulatory role in osteoblast differentiation and production of inflammatory markers. The uptake of MVs by osteoblasts was found to involve a vesicular transport mechanism. These findings support the hypothesis that osteoblast-derived MVs participate in the regulation of osteogenesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell & Tissue Engineering
Arseniy Lobov, Polina Kuchur, Aigul Khizhina, Anastasia Kotova, Andrei Ivashkin, Daria Kostina, Polina Klausen, Evgeniia Khokhlova, Egor Repkin, Kseniia Postnikova, Daria Perepletchikova, Evgeny Denisov, Tatiana Gerashchenko, Rashid Tikhilov, Svetlana Bozhkova, Andrey Sereda, Vitaliy Karelkin, Natella Enukashvily, Anna Malashicheva
Summary: In this study, we conducted a comparative analysis of mesenchymal cell primary cultures from various sources and found differences in their ability to undergo osteogenic differentiation and produce extracellular matrix. Based on the analysis of secretome, proteome, and transcriptome, we classified them into dental stem cells, mesenchymal stem cells, and fetal stem cells. Although they have differences, mesenchymal cells from all sources secreted core bone extracellular matrix-associated proteins. These findings shed light on the distinctive characteristics of mesenchymal stem cells and provide insights into their potential applications in regenerative medicine and the inherent diversity of mesenchymal cells in vivo.
Article
Biochemistry & Molecular Biology
Floor A. A. Ruiter, Jasia King, Sangita Swapnasrita, Stefan Giselbrecht, Roman Truckenmuller, Vanessa L. S. LaPointe, Matthew B. Baker, Aurelie Carlier
Summary: Three-dimensional cell culture in engineered hydrogels is widely used in tissue engineering and regenerative medicine, but guidelines for diffusion coefficients are lacking. In this study, we developed a multiscale practical approach to diffusion using mathematical modeling, fluorescence recovery after photobleaching, and hydrogel diffusion experiments. Our results showed a dampening effect of the hydrogel on diffusion and the creation of a diffusion gradient. Our model, combined with experimental measurements, provides a practical reference for diffusion coefficients in real-world culture conditions, allowing for better choices in hydrogel culture conditions. The model can be further improved to study more complex hydrogel properties and secondary interactions.
Article
Cell & Tissue Engineering
Panagiota Kakni, Barry Jutten, Daniel Teixeira Oliveira Carvalho, John Penders, Roman Truckenmuller, Pamela Habibovic, Stefan Giselbrecht
Summary: The gut microbiome is crucial for proper gut function, but imbalances can lead to gastrointestinal conditions. Current models do not accurately depict in vivo situations, thus more advanced in vitro models are needed to study host-microbiome interactions. Here, we developed a novel hypoxia-tolerant apical-out small intestinal organoid model that mimics intestinal cell composition and structure. Co-cultures with anaerobic strains showed successful colonization and probiotic benefits. These organoids will help unravel unknown mechanisms and aid in the development of microbiome-related therapeutics.
JOURNAL OF TISSUE ENGINEERING
(2023)
Article
Biochemistry & Molecular Biology
Panagiota Kakni, Carmen Lopez-Iglesias, Roman Truckenmuller, Pamela Habibovic, Stefan Giselbrecht
Summary: Pluripotent stem cell-derived intestinal organoids with reversed epithelial polarity (apical-out) successfully recapitulate tissue-specific functions and show strong epithelial barrier formation, nutrient transport, active lipid metabolism, and expression of drug-metabolizing enzymes and transporters. This breakthrough enables high-throughput/high-content in vitro applications in the fields of nutrition, metabolism, and drug discovery.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2023)
Article
Engineering, Biomedical
V. P. Galvan-Chacon, D. de Melo Pereira, S. Vermeulen, H. Yuan, J. Li, P. Habibovic
Summary: This study aimed to understand the effects of the chemical composition and surface microstructure of a beta-tricalcium phosphate (TCP) ceramic on the differentiation of human mesenchymal stromal cells (hMSCs). The results showed that the original ceramic was most potent in supporting osteogenic differentiation, but some effects of surface structure in the absence of inorganics were also observed.
BIOACTIVE MATERIALS
(2023)
Article
Materials Science, Biomaterials
Martyna Nikody, Jiaping Li, Elizabeth Rosado Balmayor, Lorenzo Moroni, Pamela Habibovic
Summary: This article introduces a polymer-ceramic composite material that enhances its osteogenic properties by adding zinc, for the treatment of critical-sized bone defects that cannot heal spontaneously and require surgical intervention. The study found that the addition of zinc can promote the differentiation of osteogenic cells, enhance extracellular matrix production and mineralization, and demonstrate the potential for treating critical-sized bone defects.
BIOMATERIALS ADVANCES
(2023)
Article
Engineering, Biomedical
Danielle Baptista, Zeinab Tahmasebi Birgani, Helene Widowski, Fiona Passanha, Vasili Stylianidis, Kevin Knoops, Eva Gubbins, Cinta Iriondo, Kari-Pekka Skarp, Robbert J. Rottier, Tim G. Wolfs, Clemens van Blitterswijk, Vanessa LaPointe, Pamela Habibovic, Niki L. Reynaert, Stefan Giselbrecht, Roman Truckenmueller
Summary: This study investigated the feasibility of culturing human bronchial organoids in a polymer film-based microwell array platform. The organoids were preformed through the culture of single cells and then transferred into the microwells for further cultivation. Various microscopy techniques were employed to characterize the organoids, and the ease of micromanipulation in the microwells was demonstrated by microinjection.
MATERIALS TODAY BIO
(2023)
Article
Nanoscience & Nanotechnology
Xingzhen Zhang, Zeynep Karagoz, Sangita Swapnasrita, Pamela Habibovic, Aureilie Carlier, Sabine van Rijt
Summary: This study investigated the effect of RGD adhesion ligand global density, spacing, and clustering levels on stem cell adhesion and differentiation. It was found that highly clustered RGD-MSN-based films with increased RGD global density promoted cell adhesion and spreading. Moreover, a higher RGD ligand clustering level also promoted the expression of alkaline phosphatase in hMSCs.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Materials Science, Biomaterials
Aygul Zengin, Filipa Castro Teixeira, Tony Feliciano, Pamela Habibovic, Carlos Domingues Mota, Matthew B. Baker, Sabine van Rijt
Summary: In this study, a new type of nanocomposite bioinks containing thiolated mesoporous silica nanoparticles (MSN) was developed. The MSNs could rapidly crosslink hydrogels upon exposure to UV light, and modification of the MSNs' surface resulted in enzyme-responsive biodegradable bioinks. Addition of specific peptides enhanced cell-matrix interactions. The nanocomposite bioinks could be printed using extrusion-based bioprinting, and showed tunable mechanical and degradation properties.
BIOMATERIALS ADVANCES
(2023)
Article
Engineering, Biomedical
Francesca Giacomini, David Baiao Barata, Hoon Suk Rho, Zeinab Tahmasebi Birgani, Clemens van Blitterswijk, Stefan Giselbrecht, Roman Truckenmuller, Pamela Habibovic
Summary: Tendons are highly organized tissues that require aligned collagen fibers for proper function. This study developed a microfluidic platform that allows controlled formation and alignment of collagen fibers, leading to changes in cell morphology and marker expression in tenocytes. This research has implications for tendon regeneration and the development of physiologically relevant in vitro models.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Nanoscience & Nanotechnology
Catarina Goncalves Fonseca, Vania Silverio, David Barata, Wolfgang Giese, Holger Gerhardt, Susana Cardoso, Claudio Areias Franco
Summary: The ability of endothelial cells to respond to blood flow is crucial for the formation and maintenance of a functional vascular network. However, the molecular mechanisms underlying the response to high flow conditions are still not well understood. In this study, we developed and validated a 96-wells fluidic system for high-throughput screenings under laminar high-flow conditions. Our findings demonstrate that endothelial cells in this system align along the flow direction and upregulate KLF2 and KLF4 levels in response to fluid flow-induced shear stress. Moreover, our fluidic system allows for efficient gene knock-down compatible with automated liquid handling for high-throughput screening platforms.
MICROSYSTEMS & NANOENGINEERING
(2023)
Article
Biology
Katharina Hennig, David Hardman, David M. B. Barata, Ines I. B. B. Martins, Miguel O. Bernabeu, Edgar R. Gomes, William Roman
Summary: In this study, a quantitative contractility assay based on optogenetics and particle image velocimetry was designed to induce fiber specification in vitro. Long-term intermittent light-stimulation patterns were applied to cultured myotubes, resulting in enhanced contractile functionality and advanced maturation. This strategy can be used to study fiber specification and refine muscle disease modelling.
LIFE SCIENCE ALLIANCE
(2023)
Article
Engineering, Biomedical
Steven Vermeulen, Kevin Knoops, Hans Duimel, Maryam Parvizifard, Denis van Beurden, Carmen Lopez-Iglesias, Stefan Giselbrecht, Roman Truckenmueller, Pamela Habibovic, Zeinab Tahmasebi Birgani
Summary: This study presents a platform for creating spheroids of human mesenchymal stromal cells and demonstrates their potential application in regenerative medicine. The mineralized spheroids promote osteogenic gene expression and can be used to study small molecule perturbations and extracellular-mediated calcification.
MATERIALS TODAY BIO
(2023)