Article
Engineering, Biomedical
Chao Sun, Yinhua Dong, Jun Wei, Meng Cai, Dongfang Liang, Yongqing Fu, You Zhou, Yi Sui, Fangda Wu, Roman Mikhaylov, Hanlin Wang, Feifei Fan, Zhihua Xie, Mercedes Stringer, Zhiyong Yang, Zhenlin Wu, Liangfei Tian, Xin Yang
Summary: This study develops a highly effective acoustic stimulator working at -20 MHz to investigate the roles of acousto-mechanical stimuli in the differentiation of hESCs. Our results show that acoustic dose alters the expression of extracellular matrix and physiological activity-related genes, indicating that acoustic stimulation is an important tool in regulating the stem cells' differentiation processes in cell therapy.
ACTA BIOMATERIALIA
(2022)
Article
Cell & Tissue Engineering
Jingran Zhang, Guang Shi, Junjie Pang, Xing Zhu, Qingcai Feng, Jie Na, Wenbin Ma, Dan Liu, Songyang Zhou
Summary: Induced crotonylation in hESCs resulted in differentiation into the endodermal lineage. This induced modification led to transcriptomic changes and decreased glycolysis in hESCs.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Hematology
Chrysa Kapeni, Leslie Nitsche, Alastair M. Kilpatrick, Nicola K. Wilson, Kankan Xia, Bahar Mirshekar-Syahkal, Vashe Chandrakanthan, Camille Malouf, John E. Pimanda, Berthold Gottgens, Kristina Kirschner, Simon R. Tomlinson, Katrin Ottersbach
Summary: This study shows that the cell cycle inhibitor p57Kip2/Cdkn1c limits the number of hematopoietic stem cells by restricting the size of the sympathetic nervous system and the amount of catecholamines secreted by these cells. This regulation occurs at the sympathetic nervous system progenitor level and has a significant impact on early fetal liver colonization.
Article
Biochemistry & Molecular Biology
Edward J. Calabrese
Summary: This paper provides a thorough assessment of hormetic dose responses in embryonic stem cells, suggesting that this response may serve as a fundamental and highly conserved evolutionary strategy.
CHEMICO-BIOLOGICAL INTERACTIONS
(2022)
Article
Developmental Biology
Anna Yoney, Lu Bai, Ali H. Brivanlou, Eric D. Siggia
Summary: Embryogenesis is guided by a limited set of signaling pathways that control the differentiation of embryonic cells to specific cell types. Recent research has found that WNT signaling primes cells for subsequent signaling responses by inducing the expression of the SMAD2 co-factor EOMES, which is sufficient to replace WNT signaling in the differentiation process.
Article
Developmental Biology
Anna Yoney, Lu Bai, Ali H. Brivanlou, Eric D. Siggla
Summary: Embryogenesis is guided by a limited set of signaling pathways dynamically expressed in different places, and in vitro models of human embryos derived from hESCs allow for addressing developmental questions. WNT priming induces the expression of the SMAD2 co-factor EOMES, enabling activin-mediated ME differentiation, revealing the mechanistic basis for priming and cellular memory in early development.
Article
Cell & Tissue Engineering
Hye Ji Moon, Na Yeon Lee, Eun Kyoung Do, Seo Yul Lee, Gyu Tae Park, Jae Kyong Lim, Jeong Kon Seo, Jae Ho Kim
Summary: In this study, Kap1 was identified as a novel Lin28A-binding protein, which regulates the stability of Lin28A through modulating the Trim71-mediated ubiquitination and degradation of Lin28A, thus playing a crucial role in the regulation of ESC self-renewal and pluripotency.
Article
Cell Biology
Maria Giovanna Scioli, Gabriele Storti, Alessandra Bielli, Massimo Sanchez, Manuel Scimeca, Jeffrey M. Gimble, Valerio Cervelli, Augusto Orlandi
Summary: The study reveals the crucial role of CD146 in ASC properties and osteochondrogenic commitment, with CD146(+) ASCs showing superior chondrogenic potential and CD146(-) ASCs demonstrating greater osteogenic capacity.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Chenge Xin, Chaonan Zhu, Ying Jin, Hui Li
Summary: This study highlights the critical role of the VEGF signaling pathway in the mesendodermal induction of hESCs, along with the important involvement of the MEK-ERK cascade in this process. Furthermore, it suggests potential crosstalk between the VEGF signaling pathway and other known pathways in mesendodermal differentiation. These findings offer new insights and approaches for the study of hESCs.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Xuepeng Wang, Chengcheng Song, Ying Ye, Yashi Gu, Xuemei Li, Peixin Chen, Dongliang Leng, Jing Xiao, Hao Wu, Sisi Xie, Weiwei Liu, Qi Zhao, Di Chen, Xi Chen, Qiang Wu, Guokai Chen, Wensheng Zhang
Summary: BRD9 plays a crucial role in the regulation of self-renewal and differentiation of human embryonic stem cells, and is involved in the progression of pancreatic and breast cancers.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Biochemical Research Methods
Jiekai Yin, Tianyu F. Qi, Yen-Yu Yang, Madeline Vera-Colon, Nicole I. zur Nieden, Yinsheng Wang
Summary: RNA modifications and their associated proteins play a regulatory role in osteogenesis, and this study provides comprehensive quantitative assessment of these proteins during osteogenic differentiation. The down-regulation of certain RNA modification proteins, mainly involved in methylation and pseudouridylation, was found. Protein-protein interaction analysis revealed their significant associations with osteogenesis-related proteins, and gene set enrichment analysis suggested a potential role of METTL1 in osteogenesis through the cytokine network.
JOURNAL OF PROTEOME RESEARCH
(2023)
Article
Cell Biology
Erika Cosset, Youssef Hibaoui, Sten Ilmjarv, Pierre-Yves Dietrich, Caroline Tapparel, Karl-Heinz Krause
Summary: This study presents a standardized protocol for differentiating human embryonic stem cells into engineered neural tissue enriched with motor neurons, and highlights the role of motor neurons in poliovirus infection within these tissues. The research also reveals the molecular events occurring during PV-3 infection in the motor neurons and emphasizes the modulation of a set of genes involved in the EGR-EP300 complex.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Roni Sarel-Gallily, Tamar Golan-Lev, Atilgan Yilmaz, Ido Sagi, Nissim Benvenisty
Summary: This study identified haploinsufficiency-related genes and identified important dosage-dependent pathways involved in hESC growth and survival.
Article
Cell & Tissue Engineering
Miao Zhang, Xin Zhao, Xiao Feng, Xiao Hu, Xuan Zhao, Wange Lu, Xinyi Lu
Summary: This study reveals the distinct roles of HIRA complex members in controlling retrotransposons and cell fate conversion in embryonic stem cells.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Biochemistry & Molecular Biology
Shotaro Kawano, Masayuki Baba, Hotaka Fukushima, Daiki Miura, Hitoshi Hashimoto, Takanobu Nakazawa
Summary: This study analyzed the role of ANK2 in the developing mouse cerebral cortex and found that ANK2 regulates neural stem cell differentiation and neuronal migration. ANK2 haploinsufficiency may impair neural development and increase the risk of ASD in patients.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Oncology
Matteo Libero Baroni, Diego Sanchez Martinez, Francisco Gutierrez Aguera, Heleia Roca Ho, Maria Castella, Samanta Zanetti, Talia Velasco Hernandez, Rafael Diaz de la Guardia, Julio Castano, Eduardo Anguita, Susana Vives, Josep Nomdedeu, Helene Lapillone, Anne E. Bras, Vincent H. J. van der Velden, Jordi Junca, Pedro Marin, Alex Bataller, Jordi Esteve, Binje Vick, Irmela Jeremias, Angel Lopez, Marc Sorigue, Clara Bueno, Pablo Menendez
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2020)
Article
Biochemistry & Molecular Biology
Nuria Serna-Pujol, Monica Salinas-Pena, Francesca Mugianesi, Natalia Lopez-Anguita, Francesc Torrent-Llagostera, Andrea Izquierdo-Bouldstridge, Marc A. Marti-Renom, Albert Jordan
Summary: The research found that G-positive bands are enriched in gene richness, active marks, and early replication, while G-negative bands are enriched in repressive marks, lamina-associated domains, and LINEs. Histone H1 variants distribute heterogeneously among G bands, and based on epigenetic features and H1 content, G bands can be organized in clusters useful for compartmentalizing the genome.
Review
Biochemistry & Molecular Biology
Marta Andres, Daniel Garcia-Gomis, Inma Ponte, Pedro Suau, Alicia Roque
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Surgery
Borja Sese, Javier M. Sanmartin, Bernat Ortega, Ramon Llull
PLASTIC AND RECONSTRUCTIVE SURGERY
(2020)
Article
Biochemistry & Molecular Biology
Inma Ponte, Marta Andres, Albert Jordan, Alicia Roque
Summary: The study reveals that transcriptional control plays a significant role in regulating histone H1, with different H1 genes showing higher expression in topologically associated domains. H1 genes are targeted by various transcription factors, indicating a fine-tuned regulation mechanism.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Article
Biology
Giorgia Maroni, Mahmoud A. Bassal, Indira Krishnan, Chee Wai Fhu, Virginia Savova, Rapolas Zilionis, Valerie A. Maymi, Nicole Pandell, Eva Csizmadia, Junyan Zhang, Barbara Storti, Julio Castano, Riccardo Panella, Jia Li, Corinne E. Gustafson, Sam Fox, Rachel D. Levy, Claire Meyerovitz, Peter J. Tramontozzi, Kimberly Vermilya, Assunta De Rienzo, Stefania Crucitta, Daniela S. Basseres, Marla Weetall, Art Branstrom, Alessandra Giorgetti, Raffaele Ciampi, Marzia Del Re, Romano Danesi, Ranieri Bizzarri, Henry Yang, Olivier Kocher, Allon M. Klein, Robert S. Welner, Raphael Bueno, Maria Cristina Magli, John G. Clohessy, Azhar Ali, Daniel G. Tenen, Elena Levantini
Summary: A tumor-specific, mutant-KRAS-associated subpopulation was identified in human and murine lung cancer, offering promise for targeted therapy developments.
COMMUNICATIONS BIOLOGY
(2021)
Article
Genetics & Heredity
Gorka Ruiz de Garibay, Ignacio Fernandez-Garcia, Sylvie Mazoyer, Flavia Leme de Calais, Pietro Ameri, Sangeetha Vijayakumar, Haydeliz Martinez-Ruiz, Francesca Damiola, Laure Barjhoux, Mads Thomassen, Lars v B. Andersen, Carmen Herranz, Francesca Mateo, Luis Palomero, Roderic Espin, Antonio Gomez, Nadia Garcia, Daniel Jimenez, Nuria Bonifaci, Ana Extremera, Julio Castano, Angel Raya, Eduardo Eyras, Xose S. Puente, Joan Brunet, Conxi Lazaro, Paolo Radice, Daniel R. Barnes, Antonis C. Antoniou, Amanda B. Spurdle, Miguel de la Hoya, Diana Baralle, Mary Helen Barcellos-Hoff, Miquel A. Pujana
Summary: Germline pathogenic variants in BRCA1 are associated with a high risk of breast and ovarian cancer. A genetic variant in intron 10, rs5820483, is linked to alternative splicing of exon 11 and the risk of breast cancer in carriers of BRCA1 pathogenic variants. The perturbation of BRCA1 exon 11 splicing may modify the breast cancer risk conferred by pathogenic variants of this gene.
Article
Cell & Tissue Engineering
Julio Castano, Damia Romero-Moya, Yvonne Richaud-Patin, Alessandra Giorgetti
Summary: The study successfully generated high-quality human induced pluripotent stem cell lines carrying two common GATA2 gene mutations using CRISPR/Cas9, providing an in vitro model for studying the molecular and cellular mechanisms underlying GATA2 deficiency.
STEM CELL RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Nuria Serna-Pujol, Monica Salinas-Pena, Francesca Mugianesi, Francois Le Dily, Marc A. Marti-Renom, Albert Jordan
Summary: Research suggests that multiple members of the histone H1 family play a significant role in chromatin compaction and regulation in human somatic cells. In breast cancer cells, silencing various H1 variants disrupts gene regulation, promotes genome-wide accessibility sites, and activates heterochromatic repeats, triggering an interferon response. This study is the first to simultaneously profile five endogenous H1 variants, providing functional evidence of genome topology alterations upon H1 depletion in human cancer cells.
NUCLEIC ACIDS RESEARCH
(2022)
Review
Nutrition & Dietetics
Maria J. Barrero, Paloma Cejas, Henry W. Long, Ana Ramirez de Molina
Summary: This review provides a comprehensive overview of the multiple layers of epigenetic interaction with food relevant for cancer prevention and treatment. The environment, including diet, has a significant impact on epigenetics, with diet being a source of metabolic substrates that influence the synthesis of cofactors or substrates for chromatin and RNA modifying enzymes. Additionally, plants are a common source of bioactives that can directly modify the activity of these enzymes.
ADVANCES IN NUTRITION
(2022)
Article
Oncology
Belen Lopez-Millan, Paula Costales, Francisco Gutierrez-Aguera, Rafael Diaz de la Guardia, Heleia Roca-Ho, Meritxell Vinyoles, Alba Rubio-Gayarre, Remi Safi, Julio Castano, Paola Alejandra Romecin, Manuel Ramirez-Orellana, Eduardo Anguita, Irmela Jeremias, Lurdes Zamora, Juan Carlos Rodriguez-Manzaneque, Clara Bueno, Francisco Moris, Pablo Menendez
Summary: The study found that EC-70124 is a promising and safe agent for the treatment of FLT3-ITDMUT AML, exhibiting robust and specific antileukemia activity while sparing healthy hematopoietic cells.
Article
Cell Biology
Joan Domingo-Reines, Gonzalo Martinez-Navajas, Rosa Montes, Mar Lamolda, Iris Simon, Julio Castano, Rosa Rios-Pelegrina, Javier Luis Lopez-Hidalgo, Raimundo Garcia del Moral, Juan A. Marchal, Pedro J. Real, Veronica Ramos-Mejia
Summary: Pediatric acute myeloid leukemia (AML) is a rare and heterogeneous disease that is the major cause of mortality in children with leukemia. Researchers report the generation and characterization of human embryonic stem cell clonal lines with inducible expression of NK5A, aiming to gain knowledge on its participation during the leukemogenic process.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Sara Perez-Luz, Jaanam Lalchandani, Nerea Matamala, Maria Jose Barrero, Sara Gil-Martin, Sheila Ramos-Del Saz, Sarai Varona, Sara Monzon, Isabel Cuesta, Iago Justo, Alberto Marcacuzco, Loreto Hierro, Cristina Garfia, Gema Gomez-Mariano, Sabina Janciauskiene, Beatriz Martinez-Delgado
Summary: Different mutations in the SERPINA1 gene lead to alpha-1 antitrypsin (AAT) deficiency and an increased risk of liver diseases. The Glu342Lys mutation causes Z-AAT polymerization and intrahepatic accumulation, resulting in liver alterations such as steatosis, fibrosis, cirrhosis, and hepatocarcinoma. This study investigated lipid status in hepatocytes carrying the Z and M alleles of the SERPINA1 gene, using hepatic organoids to assess lipid alterations. The data revealed a relationship between intrahepatic Z-AAT accumulation and lipid homeostasis changes, suggesting that liver organoids are a valuable model for studying liver diseases associated with SERPINA1 gene mutations.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Genetics & Heredity
Beatriz Martinez-Delgado, Maria J. Barrero
Summary: Rare diseases affect over 300 million people worldwide, but diagnosing them remains a major challenge with only a small percentage of patients finding the molecular cause through genetic testing. Therefore, approaches based on functional aspects of the genome are becoming increasingly important.
