Article
Dentistry, Oral Surgery & Medicine
Jia-lu Chen, Yue Tong, Qin Zhu, Lan-qing Gao, Ying Sun
Summary: The study found that Porphyromonas gingivalis LPS promoted the formation of NETs and increased extracellular DNA levels, enhancing the bactericidal activity of neutrophils. Additionally, P. gingivalis LPS also increased intracellular Ca2+ levels in neutrophils and induced the formation of NETs via a Ca2+-TPL2-MEK-ERK-PAD4 signaling pathway, contributing to the elimination of P. gingivalis.
ARCHIVES OF ORAL BIOLOGY
(2022)
Article
Dentistry, Oral Surgery & Medicine
Jia-lu Chen, Yue Tong, Qin Zhu, Lan-qing Gao, Ying Sun
Summary: The study revealed that P. gingivalis LPS promotes the formation of NETs, enhancing bactericidal activity of neutrophils. Additionally, through a series of signaling pathways, NETs contribute to the elimination of P. gingivalis.
ARCHIVES OF ORAL BIOLOGY
(2022)
Article
Immunology
Yue Tong, Yue Xin, Lanqing Fu, Jia Shi, Ying Sun
Summary: This study aimed to investigate the roles and mechanisms of NET formation in high glucose inflammatory microenvironment. The study found that Porphyromonas gingivalis (P. gingivalis) lipopolysaccharide (LPS) or high glucose could induce the formation of NETs, and this formation was enhanced in high glucose inflammatory microenvironment. Formation of NETs in high glucose inflammatory microenvironment was enhanced via oxidative stress, which further aggravated the subsequent inflammatory responses.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
Chi Zhang, Chenrong Xu, Li Gao, Xiting Li, Chuanjiang Zhao
Summary: The study revealed that P. gingivalis LPS enhances Th17 cell response by upregulating Dll-4 on CD14(+) monocytes, and this phenomenon is mainly dependent on cell-cell contact rather than cytokines.
Review
Microbiology
William A. Chen, Yuetan Dou, Hansel M. Fletcher, Danilo S. Boskovic
Summary: P. gingivalis is a gram-negative anaerobe that causes periodontitis. It disrupts commensal interactions in the oral cavity to induce a dysbiotic, proinflammatory state. P. gingivalis avoids complete host immunosuppression to take advantage of inflammation-induced tissue damage for robust bacterial growth.
Article
Microbiology
Mariusz Madej, Zuzanna Nowakowska, Miroslaw Ksiazek, Anna M. Lasica, Danuta Mizgalska, Magdalena Nowak, Anna Jacula, Monika Bzowska, Carsten Scavenius, Jan J. Enghild, Joseph Aduse-Opoku, Michael A. Curtis, F. Xavier Gomis-Ruth, Jan Potempa
Summary: The T9SS component PorZ interacts with sortase and specifically binds A-LPS, mediated by a phosphorylated branched mannan repeat in A-LPS polysaccharide. ALPS-bound PorZ interacts with sortase with significantly higher affinity, facilitating modification of cargo proteins by the cell surface attachment complex of the T9SS.
Article
Immunology
Banndith Cheat, Coralie Torrens, Asmaa Foda, Brigitte Baroukh, Jeremy Sadoine, Lotfi Slimani, Veronique Witko-Sarsat, Olivier Huck, Marjolaine Gosset, Jerome Bouchet
Summary: Our study investigates the role of NLRP3 in periodontitis, demonstrating its ambiguous role in neutrophils and its interaction with Porphyromonas gingivalis. The study reveals that NLRP3 is involved in regulatory pathways that limit periodontitis in mouse models, but its protective effect is diminished in the presence of P. gingivalis. Overexpression of NLRP3 in connective tissue leads to increased production of mature IL-1 beta and mobilization of neutrophils, while NLRP3 deficiency results in lower IL-1 beta expression and almost no neutrophil mobilization.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Tetsuya Tamura, Ruoqi Zhai, Tasuku Takemura, Kazuhisa Ouhara, Yuri Taniguchi, Yuta Hamamoto, Ryousuke Fujimori, Mikihito Kajiya, Shinji Matsuda, Syuichi Munenaga, Tsuyoshi Fujita, Noriyoshi Mizuno
Summary: The study demonstrates that geniposidic acid (GPA) has anti-inflammatory effects and can inhibit Porphyromonas gingivalis-induced periodontitis, suggesting its potential in preventing periodontal disease.
Article
Microbiology
Anna Gasiorek, Ewelina Dobosz, Barbara Potempa, Izabela Ciaston, Mateusz Wilamowski, Zuzanna Oruba, Richard J. Lamont, Jolanta Jura, Jan Potempa, Joanna Koziel
Summary: Periodontal disease is an inflammatory disease of the supporting tissues of the teeth caused by dysbiotic biofilm on the tooth surface. The negative regulator MCPIP-1 plays a key role in controlling inflammatory responses, but its stability can be influenced by periodontal pathogens like Porphyromonas gingivalis. The proteolytic activity of gingipains from these pathogens can lead to rapid degradation of MCPIP-1, exacerbating the inflammatory response. This provides insights into potential new treatment strategies for periodontitis.
Article
Dentistry, Oral Surgery & Medicine
Shuwei Qiao, Baosheng Li, Qing Cai, Zhen Li, Zhaoyi Yin, Jie He, Yuyang Li, Weiyan Meng
Summary: Ferroptosis is involved in the inflammatory processes in HGFs upon P. gingivalis-LPS stimulation. This study found that P. gingivalis-LPS induces ferroptosis in HGFs and that ferroptosis is positively correlated with inflammation levels. Ferroptosis was also observed in the gingival tissue of periodontitis rats.
Article
Cell & Tissue Engineering
Jingjun Yu, Shuangshuang Chen, Shuang Lei, Fulong Li, Yan Wang, Xiufang Shu, Wanlin Xu, Xiaolin Tang
Summary: This article discusses the use of MSCs in tissue regeneration for periodontitis, as well as their immune and inflammatory responses when exposed to P. gingivalis. It highlights the differences in MSC sources and the potential pitfalls in bone regeneration, along with mechanisms involved in inflammatory, immune responses, and osteogenic potential when exposed to P. gingivalis and its virulence factors.
STEM CELLS AND DEVELOPMENT
(2021)
Article
Biochemistry & Molecular Biology
Pablo Veloso, Alejandra Fernandez, Jessica Astorga, David Gonzalez-Quintanilla, Alfredo Castro, Alejandro Escobar, Anilei Hoare, Marcela Hernandez
Summary: The study found that Pg LPS can induce a macrophage M1 phenotype (pro-inflammatory) while Pe LPS does not elicit a significant response. Additionally, the expressions of TLR2 and TLR4 were not modified by Pg or Pe LPS.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Yanjing Ou, Mingdong Yan, Guanglin Gao, Wenjie Wang, Qiaoqiao Lu, Jiang Chen
Summary: This study demonstrates the potential of orally administered cinnamaldehyde (CA) in controlling periodontitis by inhibiting bone resorption, anaerobic bacteria accumulation, and immunoinflammatory responses. CA also shows inhibitory effects on cytokine expression, cell senescence, and promotes osteogenic differentiation.
Article
Biochemistry & Molecular Biology
Zsombor Prucsi, Agnieszka Zimny, Alicja Plonczynska, Natalia Zubrzycka, Jan Potempa, Maja Sochalska
Summary: Periodontitis is a chronic inflammatory disease caused by dysbiotic oral microbiome, and Porphyromonas gingivalis is identified as a key pathogen. The mechanism by which P. gingivalis secretes the enzyme PPAD to contribute to the inflammation loop is not fully understood. This study reveals that PPAD promotes neutrophil survival and pro-inflammatory cytokine secretion, while enhancing the resilience of bacteria to phagocytosis, thus sustaining a chronic inflammatory state.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Immunology
Qingsong Jiang, Yuxi Zhao, Yusen Shui, Xuedong Zhou, Lei Cheng, Biao Ren, Zhu Chen, Mingyun Li
Summary: Late-onset periodontitis is closely related to the interactions between neutrophils and periodontal pathogens, where the balance between neutrophils and the microbial community is crucial. Neutrophils clear pathogens mainly through phagocytosis and release mechanisms, while pathogens evade neutrophil-mediated killing through various strategies.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)