Article
Chemistry, Physical
Michael Kotik, Katerina Brodsky, Petr Halada, Hana Javurkova, Helena Pelantova, Dorota Konvalinkova, Pavla Bojarova, Vladimir Kren
Summary: Two newly identified wild-type rutinosidases from glycoside hydrolase family 5-23 can glycosylate inorganic azide with rutin as a donor, producing rutinosyl alpha-azide. The catalytic nucleophile mutant of rutinosidase from Aspergillus niger, also belonging to GH5-23, was able to synthesize rutinosyl beta-azide. Thus, both anomers of rutinosyl azide can be synthesized at a preparatory scale using either wild-type or mutant rutinosidases of GH5-23.
CATALYSIS COMMUNICATIONS
(2021)
Article
Chemistry, Physical
Alfons K. G. Felice, Christian Schuster, Alan Kadek, Frantisek Filandr, Christophe V. F. P. Laurent, Stefan Scheiblbrandner, Lorenz Schwaiger, Franziska Schachinger, Daniel Kracher, Christoph Sygmund, Petr Man, Petr Halada, Chris Oostenbrink, Roland Ludwig
Summary: This study elucidates the role of the cytochrome (CYT) domain of cellobiose dehydrogenase (CDH) in two electron transfer steps, revealing key factors and protein conformational changes in interdomain electron transfer.
Article
Infectious Diseases
Idris Mhaidi, Mouad Ait Kbaich, Sofia El Kacem, Othmane Daoui, Khadija Akarid, Tatiana Spitzova, Petr Halada, Vit Dvorak, Meryem Lemrani
Summary: This study in Morocco identified sand fly fauna in Ibaraghen village and confirmed the role of P. sergenti as a vector in the Azilal focus of cutaneous leishmaniasis. The relative abundance of other potential vector species also emphasizes the risk of introducing L. infantum and L. major in the province. This is the first combined approach in Morocco to identify sand flies using both morphology and molecular methods.
TRANSBOUNDARY AND EMERGING DISEASES
(2022)
Article
Chemistry, Analytical
Marek Polak, Ghazaleh Yassaghi, Daniel Kavan, Frantisek Filandr, Jan Fiala, Zdenek Kukacka, Petr Halada, Dmitry S. Loginov, Petr Novak
Summary: The combination of covalent labeling techniques with mass spectrometry is a progressive approach for studying protein surfaces and protein-ligand interactions. In this study, the interaction interface between the DNA binding domain of the FOXO4 protein and the DNA binding element was mapped. Different approaches were used to confirm the results and compare with other studies, showing consistent findings. This method is important for understanding the structure and dynamics of protein-DNA interactions.
ANALYTICAL CHEMISTRY
(2022)
Article
Microbiology
Viola Vankova Hausnerova, Olga Marvalova, Michaela Sikova, Mahmoud Shoman, Jarmila Havelkova, Milada Kambova, Martina Janouskova, Dilip Kumar, Petr Halada, Marek Schwarz, Libor Krasny, Jarmila Hnilicova, Josef Panek
Summary: Bacteria use small non-coding RNAs to regulate gene expression. The study identified a specific RNA called Ms1 in Actinobacteria that binds to the core of RNA polymerase and affects its intracellular level. Through computational search and gene synteny approach, the study also found Ms1 RNA candidates in other Actinobacteria, demonstrating the widespread presence of Ms1 RNA as a class of RNAP binding sRNAs.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Parasitology
Nacer Eddine Messahel, Kamel Eddine Benallal, Petr Halada, Ismail Lafri, Hemza Manseur, Ahcene Hakem, Karim Houali, Zoubir Harrat, Petr Volf, Vit Dvorak
Summary: Leishmaniases are neglected vector-borne diseases. This study in Algeria revealed the feeding preference of sand flies for cattle and the presence of cattle and dog blood in sand flies, which could play an important role in parasite transmission.
VETERINARY PARASITOLOGY- REGIONAL STUDIES AND REPORTS
(2022)
Article
Chemistry, Analytical
Ghazaleh Yassaghi, Zdenek Kukacka, Jan Fiala, Daniel Kavan, Petr Halada, Michael Volny, Petr Novak
Summary: Fast photochemical oxidation of proteins (FPOP) footprinting is a method that uses chemical reactions to label protein structures. It provides information about protein conformation, dynamics, and interactions. FPOP samples can be analyzed using either bottom-up or top-down mass spectrometry approaches. The top-down approach allows for more specific selection of protein subpopulations, but is limited to smaller proteins. Comparison with bottom-up data confirms the usefulness of the top-down approach in oxidative protein footprinting.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Lidiia Tsvik, Beate Steiner, Peter Herzog, Dietmar Haltrich, Leander Suetzl
Summary: The development of L-lactate biosensors has been hindered by the lack of diversity in L-lactate-oxidizing enzymes used as bioelements. This study utilized sequence information from protein databases to identify a wider range of enzymes for L-lactate oxidation. Additionally, a new class of soluble and FMN-dependent L-lactate dehydrogenases was discovered. These findings open up new possibilities for the construction of second-generation biosensors and other biotechnological applications.
Article
Biochemistry & Molecular Biology
Anja Kostelac, Leander Sutzl, Jolanta Puc, Valentina Furlanetto, Christina Divne, Dietmar Haltrich
Summary: This study reports the biochemical characterization of a pyranose oxidase (ScPOx) from Streptomyces canus. The results showed that ScPOx oxidizes monosaccharides and various C- and O-glycosides, and belongs to the same sequence space as the recently discovered FAD-dependent C-glycoside 3-oxidase.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Ludovika Jessica Virginia, Clemens Peterbauer
Summary: Lignin degradation in fungal systems is well characterized, but recent studies have shown the potential for lignin depolymerization and modification by bacteria. The presence of peroxidase genes in Actinobacteria suggests the existence of auxiliary enzymes like pyranose oxidase (POx). Analysis of the genomic locus of POx from Kitasatospora aureofaciens revealed an additional sequence that could serve as a Tat-signal peptide. Expression of KaPOx with this additional sequence in Streptomyces lividans confirmed its natural extracellular localization and potential role in lignin degradation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Infectious Diseases
Sineenat Sripattanakul, Teerasak Prapong, Attapon Kamlangdee, Gerd Katzenmeier, Dietmar Haltrich, Ratchanee Hongprayoon, Siriwan Prapong
Summary: Researchers have developed an indirect ELISA assay for the diagnosis of canine leptospiral infections using peptides derived from a recombinant LRR protein of Leptospira. The study showed that four peptides had high specificity and sensitivity, making them suitable for detection of antibodies against Leptospira in Thai dogs.
TROPICAL MEDICINE AND INFECTIOUS DISEASE
(2022)
Article
Infectious Diseases
Sineenat Sripattanakul, Kanpapat Boonchuay, Teerasak Prapong, Worawidh Wajjwalku, Gerd Katzenmeier, Dietmar Haltrich, Ratchanee Hongprayoon, Siriwan Prapong
Summary: The lateral flow strip test using the rhKU_Sej_LRR_2271 protein demonstrated good potential in detecting anti-leptospiral IgG in dogs, with a significant association with LipL32 ELISA. However, its sensitivity, specificity, and accuracy were relatively low.
TROPICAL MEDICINE AND INFECTIOUS DISEASE
(2022)
Article
Biochemistry & Molecular Biology
Phitsanu Pinmanee, Kamonwan Sompinit, Angkana Jantimaporn, Mattaka Khongkow, Dietmar Haltrich, Thidarat Nimchua, Prakit Sukyai
Summary: This study purified SOD from Saccharomyces cerevisiae TBRC657, resulting in purified Mn-SOD with a molecular weight of 25 kDa. The purified SOD showed optimal activity at pH 7.0 and 35°C, but had poor stability under high temperatures. However, when immobilized on BC, the stability was enhanced. Immobilized SOD demonstrated the ability to regulate ROS levels inside cells, making it a promising candidate for antioxidant applications in various industries.
Review
Food Science & Technology
Sudarma Dita Wijayanti, Lidiia Tsvik, Dietmar Haltrich
Summary: Food analysis and control are crucial for ensuring the quality and safety of food products. Electrochemical biosensors based on enzymes offer high selectivity, sensitivity, short analysis time, and cost-effectiveness, making them promising tools for food analysis.
Article
Biochemistry & Molecular Biology
Mai Ngoc Hoang, Clemens Peterbauer
Summary: Lactococcus lactis can be used as a drug delivery vector by displaying recombinant proteins on its surface, enabling painless administration and triggering robust immune response. A double-labeling method using specific dyes allows simultaneous detection and quantification of targeted proteins on the cell surface and in the cytoplasm, providing a convenient tool for monitoring the processes for cell surface display applications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
