Article
Peripheral Vascular Disease
Yusuke Tobe, Jun Homma, Katsuhisa Sakaguchi, Hidekazu Sekine, Kiyotaka Iwasaki, Tatsuya Shimizu
Summary: This study proposes a method for introducing perfusable vascular networks into 3D tissues. The researchers create an artificial vascular bed using a hydrogel that allows perfusion of the culture medium. They demonstrate the formation of numerous vascular networks in cell sheets, which can be perfused with ink or blood. This technology has the potential to be used in regenerative therapies and in vitro experimental models.
MICROVASCULAR RESEARCH
(2022)
Article
Engineering, Biomedical
Sangil Min, David Cleveland, In Kap Ko, Ji Hyun Kim, Hee Jo Yang, Anthony Atala, James J. Yoo
Summary: By incorporating vascular endothelial growth factor (VEGF) into the vascular scaffold, improved angiogenic capability was achieved, promoting vascularization, reducing apoptosis of implanted cells, and forming hybrid renal tubule-like structures.
ACTA BIOMATERIALIA
(2021)
Article
Engineering, Biomedical
Won-Woo Cho, Byoung Soo Kim, Minjun Ahn, Yeon Hee Ryu, Dong-Heon Ha, Jeong Sik Kong, Jong-Won Rhie, Dong-Woo Cho
Summary: This study introduces a new concept of assembling cell-laden tissue modules for soft tissue engineering, aiming to achieve nonhypoxic, flexible, and volume-stable tissue assembly. The significance of engineered tissue assembly is demonstrated through various evaluations, showing stable volume and significant neovascularization/adipogenesis in the implanted assembly. The work presents a novel approach of cell printing-based tissue assembly for functional reconstruction of soft tissue.
ADVANCED HEALTHCARE MATERIALS
(2021)
Article
Chemistry, Multidisciplinary
Jeong Hun Park, Hyun-Ji Park, Sarah Jo Tucker, Sarah R. Rutledge, Lizhen Wang, Michael E. Davis, Scott J. Hollister
Summary: A successful 3D printing of a novel 3D architected auxetic for large-volume soft tissue engineering is reported. The 3D auxetic design is analyzed through finite element (FE) simulation and created by selective laser sintering (SLS) of Poly-epsilon-caprolactone (PCL) for further mechanical and biological analysis. The 3D auxetic exhibits high flexibility and nonlinear stress-strain response, which is achieved despite the relatively stiff and linear properties of PCL. The excellent mechanical and biological performance of the 3D auxetic is attributed to the synergistic effect of its novel design and the printing characteristic of SLS.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Engineering, Biomedical
Zheng-Tian Xie, Jinfeng Zeng, Shigeru Miyagawa, Yoshiki Sawa, Michiya Matsusaki
Summary: 3D printing is a powerful technology for fabricating organ structures, but it faces challenges in producing large-size tissues and using multiple materials. Inspired by 3D puzzle toys, researchers have developed a building block-based printing strategy that assembles small bio-blocks into large and complex bioproducts. They successfully prepared structures that are difficult to print using conventional methods, such as puzzles with different materials and colors, a hollow collagen soccer ball, and even a full-size human heart model. This strategy also allows for the combination of various cells in a specified order, enabling investigation of cell interactions. It offers an alternative approach for tissue engineering applications.
MATERIALS TODAY BIO
(2023)
Article
Chemistry, Physical
Byoung Soo Kim, Won-Woo Cho, Ge Gao, Minjun Ahn, Jongmin Kim, Dong-Woo Cho
Summary: During tumor progression, the size and location of the tumor play a significant role in determining its metastatic potential. A novel tissue-level platform using in situ 3D cell printing was presented to study cancer-vascular interactions and investigate metastasis-associated changes. The platform enables precise positioning control and may open new possibilities for precision cancer medicine.
Article
Engineering, Biomedical
Xin Liu, Xinhuan Wang, Liming Zhang, Lulu Sun, Heran Wang, Hao Zhao, Zhengtao Zhang, Wenli Liu, Yiming Huang, Shen Ji, Jingjinqiu Zhang, Kai Li, Biaobiao Song, Chun Li, Hui Zhang, Song Li, Shu Wang, Xiongfei Zheng, Qi Gu
Summary: This study presents a methodology for fabricating soft vascularized tissue at centimeter scale using multimaterial bioprinting with a customized multistage-temperature-control printer. The printed constructs can support 3D capillary networks to mimic mature and functional liver tissue, and also enable direct surgical anastomosis of blood vessels with the printed pressure-bearing layer. This versatile platform allows for optimal cellularization in engineered tissues through the replication of vasculature networks.
ADVANCED HEALTHCARE MATERIALS
(2021)
Article
Engineering, Biomedical
Karli A. Gold, Biswajit Saha, Navaneeth Krishna Rajeeva Pandian, Brandon K. Walther, Jorge A. Palma, Javier Jo, John P. Cooke, Abhishek Jain, Akhilesh K. Gaharwar
Summary: The introduction of a new class of nanoengineered hydrogel-based cell-laden bioinks in 3D bioprinting shows promising potential for replicating the microenvironment of human vasculature, with high printability and cell protection. The ability to print anatomically accurate, multicellular blood vessels provides a valuable tool for modeling vascular function and pathophysiology, demonstrating thromboinflammatory responses similar to in vitro and in vivo models.
ADVANCED HEALTHCARE MATERIALS
(2021)
Article
Nanoscience & Nanotechnology
Shibo Li, Chengpan Li, Muhammad Imran Khan, Jing Liu, Zhengdi Shi, Dayong Gao, Bensheng Qiu, Weiping Ding
Summary: This study reports an approach for constructing hepatic sinusoids. Hepatic sinusoids are formed by demolding a self-developed microneedle array from a photocurable cell-loaded matrix in a large-scale liver-acinus-chip microsystem with a designed dual blood supply. The construction of hepatic sinusoids significantly enhances interstitial flows, leading to high cell viability, liver microstructure formation, and enhanced hepatocyte metabolism. Additionally, the study demonstrates the effects of oxygen and glucose gradients on hepatocyte functions and the application of the chip in drug testing. This work paves the way for the biofabrication of fully functionalized large-scale liver bioreactors.
MICROSYSTEMS & NANOENGINEERING
(2023)
Article
Engineering, Biomedical
Lior Debbi, Barak Zohar, Margarita Shuhmaher, Yulia Shandalov, Idit Goldfracht, Shulamit Levenberg
Summary: By combining living cells, biological hydrogel, and biodegradable synthetic polymer, a multi-scale vascular network within thick, implantable engineered tissues was successfully fabricated in this study. The functionality and patency of the multi-scale vascular network were demonstrated by dextran passage and physiological flow conditions, leading to significant improvements in blood perfusion compared to control micro-scale-vascularized grafts. This innovative approach of designing and fabricating multi-scale vascular architectures within 3D engineered tissues shows promise for both in vitro models and therapeutic translation research.
Article
Engineering, Biomedical
Melis Isik, Ece Karakaya, Tugba Sezgin Arslan, Deniz Atila, Yasar Kemal Erdogan, Yavuz Emre Arslan, Hakan Eskizengin, Cemil Can Eylem, Emirhan Nemutlu, Batur Ercan, Matteo D'Este, Babatunde O. Okesola, Burak Derkus
Summary: 3D printing provides an exciting opportunity for fabricating biomedical constructs with specific geometries, sizes, and functions, but is limited by the range of printable and bio-instructive materials. This study reports on the development of printable and perfusable multicomponent hydrogel constructs with high elasticity, self-recovery properties, excellent hydrodynamic performance, and improved bioactivity. The constructs were created using a design strategy that integrates fast gelation kinetics, in situ crosslinking, and temperature-dependent self-assembly. In vitro and pre-clinical models demonstrated the pro-angiogenic and anti-inflammatory properties of the constructs, showcasing their potential applications in vascular tissue engineering and regenerative medicine.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Yongcong Fang, Yihan Guo, Bingyan Wu, Zibo Liu, Min Ye, Yuanyuan Xu, Mengke Ji, Li Chen, Bingchuan Lu, Kaiji Nie, Zixuan Wang, Jianbin Luo, Ting Zhang, Wei Sun, Zhuo Xiong
Summary: A reversible ink template-based bioprinting strategy (SPIRIT) is developed to replicate the external geometry and internal structure of complex organs. This strategy utilizes a microgel-based bioink with shear-thinning and self-healing behavior to support embedded 3D printing, resulting in the generation of cardiac tissues and organoids. This technique allows for the rapid replication of complex organ geometry and internal structures, accelerating the biofabrication and therapeutic applications of tissue and organ constructs.
ADVANCED MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Isabel Orellano, Alexander Thomas, Aaron Herrera, Erik Brauer, Dag Wulsten, Ansgar Petersen, Lutz Kloke, Georg N. Duda
Summary: The research introduces a bioengineering approach that utilizes 3D bioprinting to control the formation of microvascular structures by guiding cellular self-assembly. The constructed vascular networks display regulated distribution, network orientation, vessel length and branching behavior, as well as developed lumens and signs of vascular stabilization. This novel biofabrication approach shows the capability to generate distinctly vascularized constructs with controlled microvascular network formation, which could be significant in the development of vascularized implants or in vitro screening models.
ADVANCED FUNCTIONAL MATERIALS
(2022)
Article
Chemistry, Multidisciplinary
Yiming Huang, Hao Zhao, Xinhuan Wang, Xin Liu, Zhiqiang Gao, Haotian Bai, Fengting Lv, Qi Gu, Shu Wang
Summary: A polyurethane-gelatin methacryloyl (PU-GelMA) hybrid ink with photo-crosslinkable elastic hydrogel properties was developed for 3D printing. The ink's elasticity and printability can be adjusted by adding acrylic monomers. It can be used for either direct extrusion printing or printing with sacrificial scaffolds to create vascular-like structures. The proliferation of endothelial cells on the PU-GelMA hydrogel suggests its biocompatibility and potential use in artificial vessels.
CHEMICAL COMMUNICATIONS
(2022)
Article
Engineering, Biomedical
Xin Liu, Yan Li, Yuxiang Sun, Bo Chen, Wenxian Du, Yuehua Li, Ning Gu
Summary: The well-designed functional magnetic scaffold (MDP) with a temperature-controlled switch promotes macrophages migration and M2 polarization, facilitating rapid reendothelialization and vascular repair.
