Journal
PLOS ONE
Volume 11, Issue 5, Pages -Publisher
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pone.0155687
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Funding
- Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan
- Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan [23115501, 25115701, 15H01413, 15H01415]
- MEXT, Japan [25670103, 15K15025]
- JSPS KAKENHI [25250001, 26293213, 25290002]
- Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST)
- Japan Society for the Promotion of Science (JSPS) [15J05551]
- Tohoku University Institute for International Advanced Research and Education (IIARE)
- Subsidies for Private Universities (Showa University of Medicine and Hyogo College of Medicine)
- Grants-in-Aid for Scientific Research [26293213, 25670103, 25250001, 16K07003, 15H01415, 15KK0331, 15K06696, 25115701, 15K15025, 15H01413, 15J05551] Funding Source: KAKEN
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Despite the strength of the Cre/loxP recombination system in animal models, its application in rats trails that in mice because of the lack of relevant reporter strains. Here, we generated a floxed STOP tdTomato rat that conditionally expresses a red fluorescent protein variant (tdTomato) in the presence of exogenous Cre recombinase. The tdTomato signal vividly visualizes neurons including their projection fibers and spines without any histological enhancement. In addition, a transgenic rat line (FLAME) that ubiquitously expresses tdTomato was successfully established by injecting intracytoplasmic Cre mRNA into fertilized ova. Our rat reporter system will facilitate connectome studies as well as the visualization of the fine structures of genetically identified cells for long periods both in vivo and ex vivo. Furthermore, FLAME is an ideal model for organ transplantation research owing to improved traceability of cells/tissues.
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