Article
Biochemistry & Molecular Biology
Lavinia-Lorena Pruteanu, Cornelia Braicu, Dezso Modos, Maria-Ancuta Jurj, Lajos-Zsolt Raduly, Oana Zanoaga, Lorand Magdo, Roxana Cojocneanu, Sergiu Pasca, Cristian Moldovan, Alin Iulian Moldovan, Adrian Bogdan Tigu, Eugen Gurzau, Lorentz Jantschi, Andreas Bender, Ioana Berindan-Neagoe
Summary: This study evaluated the effect of arsenate on triple negative breast cancer (TNBC) cells and found that arsenate induced cytoskeletal alteration, autophagy, and apoptosis. TNBC cells showed a more prominent response to arsenate compared to other cell lines.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Yongxian Zhuang, Jordan M. Grainger, Peter T. Vedell, Jia Yu, Ann M. Moyer, Huanyao Gao, Xiao-Yang Fan, Sisi Qin, Duan Liu, Krishna R. Kalari, Matthew P. Goetz, Judy C. Boughey, Richard M. Weinshilboum, Liewei Wang
Summary: The study successfully established two breast cancer cell lines with unique genomic characteristics derived from patient-derived xenografts. These cell lines retained the genetic and drug response information of the patients, providing a valuable tool for understanding the molecular mechanisms of drug resistance and exploring new treatment strategies.
Article
Multidisciplinary Sciences
G. Gambardella, G. Viscido, B. Tumaini, A. Isacchi, R. Bosotti, D. di Bernardo
Summary: This study characterizes the cell lines of different subtypes of breast cancer using single-cell RNA-seq and trains a deconvolution algorithm to predict drug responses in heterogeneous tumor cell populations. The findings show high heterogeneity in the expression of biomarkers within cancer cells, and transcriptional heterogeneity allows cells with differential drug sensitivity to coexist in the same population. This work provides a framework to determine tumor heterogeneity in terms of cell line composition and drug response.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Marian Kalocsay, Matthew J. Berberich, Robert A. Everley, Maulik K. Nariya, Mirra Chung, Benjamin Gaudio, Chiara Victor, Gary A. Bradshaw, Robyn J. Eisert, Marc Hafner, Peter K. Sorger, Caitlin E. Mills, Kartik Subramanian
Summary: Quantitative proteomics was performed on 60 human-derived breast cancer cell line models, resulting in the identification and characterization of subtypes of breast cancer. The datasets were found to conform to known transcriptional subtypes and are freely available for public use. These datasets can be used in combination with other measurements to predict drug response, provide context in signaling pathway models, and identify markers of sensitivity or resistance to therapeutics.
Article
Multidisciplinary Sciences
Micaela Giani, Yoel Genaro Montoyo-Pujol, Gloria Peiro, Rosa Maria Martinez-Espinosa
Summary: Oxidative stress is linked to neoplasia, but antioxidants can help prevent it. In this study, the cytotoxic effect of BRCE from Haloferax mediterranei on breast cancer cell lines was evaluated. The results showed that BRCE selectively affected breast cancer cells, particularly triple-negative subtypes, and also had an impact on cell size.
SCIENTIFIC REPORTS
(2023)
Review
Medicine, Research & Experimental
C. Pawan K. Patro, Nupur Biswas, Sandeep C. Pingle, Feng Lin, Misa Anekoji, Lawrence D. Jones, Santosh Kesari, Feng Wang, Shashaanka Ashili
Summary: This article reviews the potential therapeutic approach of 5 & PRIME;-methylthioadenosine phosphorylase (MTAP) gene loss for treating glioblastoma. It discusses the relevance of MTAP loss in mitigating the effects of glioblastoma and explores various aspects of MTAP loss in the field of glioblastoma therapeutics.
JOURNAL OF TRANSLATIONAL MEDICINE
(2022)
Article
Chemistry, Analytical
Kevin Saruni Tipatet, Liam Davison-Gates, Thomas Johann Tewes, Emmanuel Kwasi Fiagbedzi, Alistair Elfick, Bjorn Neu, Andrew Downes
Summary: In this study, biochemical changes associated with acquired radioresistance in breast cancer cells were successfully characterized using Raman spectroscopy and machine learning, with 100% accuracy in distinguishing between wild-type and acquired radioresistant cells. The study also found similar changes in chemical composition in hormone receptor positive and negative cells with acquired radioresistance, suggesting the potential for tailored clinical treatments based on Raman signals of radioresistance in tumors or biopsies.
Article
Food Science & Technology
Semih Dalkilic, Ismail Korkmaz, Lutfiye Kadioglu Dalkilic, Gokhan Akay, Songul Fidan
Summary: Cancer is a major cause of worldwide deaths, with increasing incidence and low survival rates. Researchers are actively studying new diagnostic methods, candidate drug molecules, and molecular pathways for targeted new drugs. In this study, the cytotoxic and apoptotic effects of Smilax aspera L. root extracts were investigated for the first time, showing significant cytotoxic effects on three different cancer cell lines and inducing apoptosis in one cell line. This highlights the strategic importance of S. aspera in cancer treatment.
Article
Pharmacology & Pharmacy
Verona Buocikova, Silvia Tyciakova, Eleftherios Pilalis, Chara Mastrokalou, Maria Urbanova, Miroslava Matuskova, Lucia Demkova, Veronika Medova, Eleonora Marta Longhin, Elise Runden-Pran, Maria Dusinska, Ivan Rios-Mondragon, Mihaela Roxana Cimpan, Alena Gabelova, Andrea Soltysova, Bozena Smolkova, Aristotelis Chatziioannou
Summary: DAC, in combination with traditional anticancer drugs, shows promise as a treatment option for solid tumors. Increasing DCK expression to potentiate DAC's effect did not lead to changes in global DNA methylation levels or cell viability.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Polymer Science
Beste Cagdas Tunali, Omer Akturk, Durukan Sahingoz, Mustafa Turk, Ayten Celebi Keskin
Summary: In this study, polyethyleneimine-capped gold nanoparticles (PEI-AuNPs) were synthesized using the pulsed laser ablation in liquid (PLAL) technique, and then combined with Bombyx mori silk fibroin (SF) to form nanocomposites (PEI-SF-AuNPs). The biocompatibility of PEI-AuNPs and PEI-SF-AuNPs in breast cancer cells MCF-7 and MDA-MB231 were tested, and it was found that PEI-AuNP1 showed the least cytotoxicity. Therefore, PEI-AuNP1 nanocomposites can be suggested as siRNA carriers in gene-based breast cancer therapies.
EUROPEAN POLYMER JOURNAL
(2023)
Article
Chemistry, Multidisciplinary
Rana Abu-Dahab, Nouf N. Mahmoud, Maha Abdallah, Lama Hamadneh, Suhair Hikmat, Rand Zaza, Duaa Abuarqoub, Enam A. Khalil
Summary: Surface-decorated gold nanorods exhibit varying antiproliferative effects against different breast cancer cell lines, with MDA-MB-231 cells showing the highest cytotoxicity and predominantly undergoing apoptosis upon treatment with GNRs. The expression of the CDH1 gene, involved in cell adhesion and metastasis, is significantly increased in treated MDA-MB-231 cells compared to other cell lines.
Article
Multidisciplinary Sciences
Xiaoyu Song, Jiayi Ji, Joseph H. Rothstein, Stacey E. Alexeeff, Lori C. Sakoda, Adriana Sistig, Ninah Achacoso, Eric Jorgenson, Alice S. Whittemore, Robert J. Klein, Laurel A. Habel, Pei Wang, Weiva Sieh
Summary: Researchers have developed a new cell-type-aware transcriptome-wide association study approach to predict cell-type level gene expression and identify disease-associated genes. This approach provides insights into the genetic and cellular etiology of diseases such as breast cancer.
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Yoel Genaro Montoyo-Pujol, Marta Garcia-Escolano, Jose J. Ponce, Silvia Delgado-Garcia, Tina Aurora Martin, Hortensia Ballester, Elena Castellon-Molla, Pascual Martinez-Peinado, Sandra Pascual-Garcia, Jose Miguel Sempere-Ortells, Gloria Peiro
Summary: Advances in immunotherapy have led to increased interest in studying the role of the immune system in breast cancer. This study evaluated the expression of various immune regulation genes in different breast cancer cell lines and mammospheres. The results showed that the expression of these genes varied depending on the breast cancer phenotype, culture conditions, and interaction with immune cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Katherine Ellis, Rachael Wood
Summary: The objective of this study was to compare the invasiveness of three available endometriotic cell lines to cancer cell lines and assess consistency across different invasion assays. The results showed that the endometriotic cell lines exhibited similar invasiveness to the cancer cell lines and there were no significant differences. The expression of E-Cadherin declined in the invaded EEC12Z cells, supporting the hypothesis of epithelial-to-mesenchymal transition influencing the invasiveness of peritoneal endometriosis cell line.
Article
Biochemistry & Molecular Biology
Neel Desai, Jeffrey S. Morris, Veerabhadran Baladandayuthapani
Summary: Cancer cell lines are used as model systems for studying therapeutic interventions. This study presents NetCellMatch, a robust framework for network-based matching of cell lines to patient tumors, which combines genomic similarity and community detection algorithms. The results demonstrate the ability of NetCellMatch to identify patient-cell line matches and potential proteomic drivers of similarity.
CHEMISTRY & BIODIVERSITY
(2022)
Review
Oncology
Francesco Schettini, Fabiola Giudici, Ottavia Bernocchi, Marianna Sirico, Silvia P. Corona, Mario Giuliano, Mariavittoria Locci, Ida Paris, Giovanni Scambia, Sabino De Placido, Pasquale Rescigno, Aleix Prat, Giuseppe Curigliano, Daniele Generali
Summary: The study demonstrated the significant role of PARPis in the treatment of metastatic solid tumors, improving PFS, ORR, and OS regardless of the presence of BRCA1/2 mutations. Subgroup analysis revealed significant differences in outcomes based on tumor site, treatment line, control arm, and type of PARPi used.
EUROPEAN JOURNAL OF CANCER
(2021)
Article
Oncology
Sara Manglaviti, Marta Brambilla, Diego Signorelli, Roberto Ferrara, Giuseppe Lo Russo, Claudia Proto, Giulia Galli, Alessandro De Toma, Mario Occhipinti, Giuseppe Viscardi, Teresa Beninato, Emma Zattarin, Marta Bini, Riccardo Lobefaro, Giacomo Massa, Achille Bottiglieri, Giulia Apollonio, Elisa Sottotetti, Rosa Maria Di Mauro, Benedetta Trevisan, Monica Ganzinelli, Alessandra Fabbri, Filippo G. M. de Braud, Marina Chiara Garassino, Arsela Prelaj
Summary: The role of ICIs in patients with uncommon histologies (UH) of non-small cell lung cancer (NSCLC) remains unclear. This retrospective study found no significant difference between UH and common histologies (CH) groups treated with ICIs. Prospective trials are needed to clarify the role of ICIs in UH patients.
CLINICAL LUNG CANCER
(2022)
Letter
Oncology
Cristina Borzi, Elisa Caiola, Monica Ganzinelli, Giovanni Centonze, Mattia Boeri, Massimo Milione, Massimo Broggini, Gabriella Sozzi, Massimo Moro
JOURNAL OF THORACIC ONCOLOGY
(2021)
Article
Oncology
Giandomenico Roviello, Valerio Nardone, Alberto Bonetta, Pierpaolo Correale, Alfredo Molteni, Maria C. Lazzari, Daniele Generali
Summary: In this study, the effects of different treatment modalities and Gleason score on the lymphocyte composition of prostate cancer patients undergoing radiotherapy were investigated. The results showed that all lymphocyte subpopulations decreased significantly after radiotherapy. The changes in several lymphocyte subpopulations were correlated with previous surgery, Gleason score, and whole pelvic radiotherapy, with whole pelvic radiotherapy showing the strongest correlation.
AMERICAN JOURNAL OF CLINICAL ONCOLOGY-CANCER CLINICAL TRIALS
(2022)
Article
Oncology
David J. Pinato, Meera Patel, Lorenza Scotti, Emeline Colomba, Saoirse Dolly, Angela Loizidou, John Chester, Uma Mukherjee, Alberto Zambelli, Alessia Dalla Pria, Juan Aguilar-Company, Mark Bower, Ramon Salazar, Alexia Bertuzzi, Joan Brunet, Matteo Lambertini, Marco Tagliamento, Anna Pous, Ailsa Sita-Lumsden, Krishnie Srikandarajah, Johann Colomba, Fanny Pommeret, Elia Segui, Daniele Generali, Salvatore Grisanti, Paolo Pedrazzoli, Gianpiero Rizzo, Michela Libertini, Charlotte Moss, Joanne S. Evans, Beth Russell, Nadia Harbeck, Bruno Vincenzi, Federica Biello, Rossella Bertulli, Diego Ottaviani, Raquel Linan, Sabrina Rossi, M. Carmen Carmona-Garcia, Carlo Tondini, Laura Fox, Alice Baggi, Vittoria Fotia, Alessandro Parisi, Giampero Porzio, Paola Queirolo, Claudia Andrea Cruz, Nadia Saoudi-Gonzalez, Eudald Felip, Ariadna Roque Lloveras, Thomas Newsom-Davis, Rachel Sharkey, Elisa Roldan, Roxana Reyes, Federica Zoratto, Irina Earnshaw, Daniela Ferrante, Javier Marco-Hernandez, Isabel Ruiz-Camps, Gianluca Gaidano, Andrea Patriarca, Riccardo Bruna, Anna Sureda, Clara Martinez-Vila, Ana Sanchez de Torre, Rossana Berardi, Raffaele Giusti, Francesca Mazzoni, Annalisa Guida, Lorenza Rimassa, Lorenzo Chiudinelli, Michela Franchi, Marco Krengli, Armando Santoro, Aleix Prat, Josep Tabernero, Mieke Van Hemelrijck, Nikolaos Diamantis, Alessandra Gennari, Alessio Cortellini
Summary: This European registry-based study found that the severity and mortality of COVID-19 in cancer patients have improved since the beginning of the pandemic, possibly due to earlier diagnosis, improved management, and dynamic changes in community transmission.
Article
Oncology
Arsela Prelaj, Mattia Boeri, Alessandro Robuschi, Roberto Ferrara, Claudia Proto, Giuseppe Lo Russo, Giulia Galli, Alessandro De Toma, Marta Brambilla, Mario Occhipinti, Sara Manglaviti, Teresa Beninato, Achille Bottiglieri, Giacomo Massa, Emma Zattarin, Rosaria Gallucci, Edoardo Gregorio Galli, Monica Ganzinelli, Gabriella Sozzi, Filippo G. M. de Braud, Marina Chiara Garassino, Marcello Restelli, Alessandra Laura Giulia Pedrocchi, Francesco Trovo
Summary: This study demonstrates the usefulness of artificial intelligence and machine learning tools in predicting the response and efficacy of immunotherapy in non-small cell lung cancer patients. By integrating multifactorial data, these tools can assist in selecting candidates for immunotherapy.
Article
Oncology
Emine Goker, Mathijs P. Hendriks, Marc Van Tilburg, Andrei Barcaru, Lorenza Mittempergher, Angela Van Egmond, Miranda Kleijn, Daniele Generali
Summary: This study aims to measure the response to neoadjuvant chemotherapy and assess the 5-year survival outcome using the molecular subgroups determined by MammaPrint and BluePrint. The results suggest that MammaPrint and BluePrint can predict chemosensitivity and 5-year outcomes more accurately than traditional pathological subtyping, supporting informed decision-making.
EUROPEAN JOURNAL OF CANCER
(2022)
Article
Environmental Sciences
Lorenzo Palamenghi, Fabiola Giudici, Guendalina Graffigna, Daniele Generali
Summary: COVID-19 had a significant impact on the Italian healthcare systems, leading to decreased trust in healthcare authorities among citizens. This study found that in an Italian city severely affected by the pandemic, citizens focused on COVID-19 symptoms and perceived lower importance and severity of other symptoms.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2022)
Article
Oncology
Michela Chiappa, Federica Guffanti, Martina Anselmi, Monica Lupi, Nicolo Panini, Lisa Wiesmuller, Giovanna Damia
Summary: Olaparib-resistant cell lines with different mechanisms of resistance were generated through in vitro drug treatment. ATR, Chk1, and Wee1 inhibitors showed synergistic effects with olaparib in both sensitive and resistant sublines, regardless of the HR status.
Article
Oncology
Emma Zattarin, Sara Manglaviti, Giulia Apollonio, Teresa Beninato, Laura Mazzeo, Giacomo Massa, Achille Bottiglieri, Edoardogregorio Galli, Alessandro De Toma, Mario Occhipinti, Marta Brambilla, Roberto Ferrara, Monica Ganzinelli, Claudia Proto, Marina Chiara Garassino, Filippo de Braud, Giuseppe Lo Russo, Arsela Prelaj
Summary: The study explored clinical and laboratory biomarkers in patients with advanced non-small-cell lung cancer (aNSCLC) receiving chemo-immunotherapy (CIT). The neutrophil-to-lymphocyte ratio was identified as an independent prognostic biomarker for worse progression-free survival and worse overall survival. The EPSILoN score divided patients into three prognostic groups, with varying median overall survival.
Article
Oncology
Arsela Prelaj, Achille Bottiglieri, Gajanan Bhat, Rocky Washington, Giuseppina Calareso, Gabriella Francesca Greco, Roberto Ferrara, Marta Brambilla, Alessandro De Toma, Mario Occhipinti, Sara Manglaviti, Alberto Soro, Monica Ganzinelli, Giuseppe Lo Russo, Claudia Proto
Summary: Among next-generation tyrosine kinase inhibitors, poziotinib has shown effectiveness and good tolerance in patients with uncommon EFGR alterations, particularly those with EGFR exon 20 insertion mutations in metastatic non-small-cell lung cancer.
FRONTIERS IN ONCOLOGY
(2022)
Article
Cell Biology
Marika Colombo, Federico Passarelli, Paola A. Corsetto, Angela M. Rizzo, Mirko Marabese, Giulia De Simone, Roberta Pastorelli, Massimo Broggini, Laura Brunelli, Elisa Caiola
Summary: Hyperactivation of the phosphatidylinositol-3-kinase (PI3K) pathway is a common event in human cancers. Metabolic alterations are closely associated with drug resistance, and altered metabolic pathways can help cancer cells adapt to continuous drug exposure and develop resistance. In this study, researchers identified that an increased unsaturation degree of lipid species is associated with increased plasma membrane fluidity and that fatty acid desaturase FADS2 mediates drug resistance in non-small cell lung cancer (NSCLC) cell lines resistant to the usual PI3K-mTOR inhibitor BEZ235.
Article
Oncology
Monica Ganzinelli, Federica Guffanti, Anna Ianza, Navid Sobhani, Sergio Crovella, Fabrizio Zanconati, Cristina Bottin, Marco Confalonieri, Stefano Fumagalli, Alessandra Guglielmi, Daniele Generali, Giovanna Damia
Summary: The study analyzed the correlation between DNA repair biomarkers and survival in malignant pleural mesothelioma (MPM) patients and found that down-regulation of DNA repair expression score is associated with longer survival. These findings could potentially lead to the development of a simple, cost-effective test for better management of MPM patients.
Meeting Abstract
Oncology
Roberto Ferrara, Elena Jachetti, Giuseppina Calareso, Marta Brambilla, Giuseppe Lo Russo, Claudia Proto, Arsela Prelaj, Diego Signorelli, Giulia Galli, Alessandro De Toma, Mario Occhipinti, Sara Manglaviti, Alice Labianca, Monica Ganzinelli, Sestina Maria Spano, Giuliano Molino, Antonia Martinetti, Francesca Gabriella Greco, Marta Bini, Teresa Beninato, Filippo de Braud, Mario Paolo Colombo, Marina Chiara Garassino, Sabrina Sangaletti
Article
Oncology
Concetta Elisa Onesti, Marco Tagliamento, Giuseppe Curigliano, Nadia Harbeck, Rupert Bartsch, Hans Wildiers, Vivianne Tjan-Heijnen, Miguel Martin, Sylvie Rottey, Daniele Generali, Mario Campone, Massimo Cristofanilli, Lajos Pusztai, Marc Peeters, Guy Berchem, Javier Cortes, Thomas Ruhstaller, Eva Ciruelos, Hope S. Rugo, Guy Jerusalem
Summary: The COVID-19 pandemic has had a significant impact on oncology units globally, resulting in delays in surgeries, treatment adaptations, and increased use of telemedicine. This impact is expected to persist in the future.
JCO GLOBAL ONCOLOGY
(2021)