Article
Chemistry, Analytical
Tingting Yan, Jiang Zhu, Ying Li, Ting He, Yunhuang Yang, Maili Liu
Summary: Aflatoxin B-1 (AFB(1)) contamination poses a significant threat to food safety and human health, requiring continuous monitoring. In this study, a biotin-streptavidin-amplified enzyme-linked immunosorbent assay (BA-ELISA) was developed for sensitive and rapid detection of AFB(1) in cereal. The BA-ELISA demonstrated improved sensitivity, reduced assay time, and significant antibody savings compared to conventional ELISA.
Article
Multidisciplinary Sciences
Youhee Heo, Kwanwoo Shin, Min Cheol Park, Ji Yoon Kang
Summary: This report introduces a method for enhancing the sensitivity of chemifluorescence-based ELISA, using photooxidation-induced fluorescence amplification (PIFA). PIFA utilizes autocatalytic photooxidation to amplify the fluorescent product, improving detection sensitivity. The method requires minimal modifications to the fluorescence reader and is compatible with conventional ELISA assays.
SCIENTIFIC REPORTS
(2021)
Article
Chemistry, Analytical
Hui Li, Menglu Li, Yucai Yang, Fei Wang, Feng Wang, Chao Li
Summary: A direct method has been described to establish the correlation between non-nucleic acid analytes and the CRISPR/Cas12a system using rationally designed DNA strands, enabling ultrasensitive detection of biomarkers from different species. The signal output is highly optional and the sensing principle is akin to ELISA, allowing for direct application on the currently available ELISA platform, further facilitating its use in medical diagnostics.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Applied
Yuechun Li, Han Zhang, Zhaowen Cui, Sijie Liu, Jingke Xu, Conghui Jia, Yaqian Chen, Lulu Wang, Jing Sun, Daohong Zhang, Mingqiang Zhu, Jianlong Wang
Summary: This research explores a novel strategy to enhance the catalytic activity of enzymes, leading to the development of a sensitive ELISA method. By utilizing a chemical staining strategy, the catalytic activity of horseradish peroxidase was directly improved, and a new CS-ELISA method for detecting clenbuterol was established. The CS-ELISA exhibited a 2-fold improvement in sensitivity and linear range compared to conventional ELISA, and demonstrated good applicability in detecting clenbuterol in pork tenderloin samples.
Article
Chemistry, Analytical
Ying Wang, Tong Bu, Yuanyuan Cao, Haiyu Wu, Jia Xi, Qinlin Feng, Chenyu Xuan, Li Wang
Summary: In this study, a peroxidase-mimicking nanozyme-improved enzyme-linked immunosorbent assay (ELISA) was developed using the bimetallic PdRu nanozyme as a catalytic carrier for detecting Escherichia coli O157:H7. The PdRu nanozyme exhibited high catalytic activity and affinity with antibody, ensuring successful establishment of a novel colorimetric biosensor. The PdRu-based ELISA achieved ultrasensitive detection sensitivity and satisfactory specificity and reproducibility, making it suitable for bioassays and clinical diagnostics.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Applied
Min Jia, Shuang Chen, Tingting Shi, Chunyang Li, Yanping Wang, Hongyan Zhang
Summary: The PBELISA method using plasmonic biomimetic technology combined with MIP film and CAT-mediated AuNPs growth achieved ultra-sensitive visual detection of BPA; with a visual limit of detection of 40 pg/mL, the method showed good dynamic linear response and high recovery rates.
Article
Materials Science, Multidisciplinary
Bo Kyeong Yoon, Tun Naw Sut, Ki Yeol Yoo, Seung Hwa Lee, Youngkyu Hwang, Joshua A. Jackman, Nam-Joon Cho
Summary: A streamlined ELISA strategy using lipid nanoparticles as a blocking agent was developed, showing fewer processing steps, quicker protocol time, and superior detection performance compared to conventional BSA blocking. This method has been successfully applied to COVID-19 antibody detection and is broadly applicable for rapid ELISA diagnostics development.
APPLIED MATERIALS TODAY
(2021)
Article
Agriculture, Dairy & Animal Science
Xudong Jing, Shan Shan, Keyu Xing, Wenkai Cao, Xiaoyue Xiao, Daofeng Liu, Weihua Lai
Summary: Traditional ELISA is not sensitive enough for detecting E. coli O157:H7, but a new method utilizing streptavidin scaffolded DNA tetrad (SS-DNAt) and fluorescence poly adenine-thymine-templated copper nanoclusters shows improved sensitivity. This fluorescence ELISA has a linear range of 104 to 106 cfu/mL, with a detection limit of 3.75 x 10(3) cfu/mL, which is 6.16 times better than traditional ELISA. The method also shows good recovery rate (86.7% to 93.6%) and low coefficient of variation (5.6% to 10.5%) in milk. This method can be used for detecting hazardous materials in food.
JOURNAL OF DAIRY SCIENCE
(2023)
Article
Food Science & Technology
Xiya Zhang, Mingyue Ding, Chensi Zhang, Yexuan Mao, Youyi Wang, Peipei Li, Haiyang Jiang, Zhanhui Wang, Xuezhi Yu
Summary: The researchers developed a rapid icELISA method for monitoring brevetoxin levels in oysters, providing a useful tool for the detection of these toxins in shellfish samples.
Article
Biotechnology & Applied Microbiology
Xinyang Li, Miyang Li, Shengbao Duan, Hongmei Wang, Yong Li, Tiemei Liu
Summary: The objective of this study is to develop a simple, rapid, reliable, sensitive, and cost-effective method for prenatal detection of fetomaternal haemorrhage by combining a multi-aperture silk membrane with enzyme-linked immunosorbent assay (ELISA). This method does not require any complicated instruments and can be visually colored, providing a new method for clinical detection of fetomaternal haemorrhage.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Chemistry, Applied
Shunsuke Fujii, Tomoe Ohta, Riho Ehama, Mizuki Irikida, Shuichi Nomura, Yukihiro Shoyama, Takuhiro Uto
Summary: This study developed a simple and reliable method for the detection and quantification of FMN in food samples, and successfully applied it in cell-based assays.
Article
Chemistry, Multidisciplinary
Ying Qin, Jing Wen, Lirong Zheng, Hongye Yan, Lei Jiao, Xiaosi Wang, Xiaoli Cai, Yu Wu, Guojuan Chen, Lijuan Chen, Liuyong Hu, Wenling Gu, Chengzhou Zhu
Summary: Single-atom catalysts, especially platinum single atoms (Pt SAs), show great potential in catalyst design, with Pt SAs supported by nanomaterials demonstrating superior performance, particularly in photoelectrochemical biosensing, enhancing detection sensitivity for specific analytes.
Article
Biochemistry & Molecular Biology
Yuan-yuan Yang, Yu Wang, Yi-feng Zhang, Feng Wang, Yi-fan Liang, Jin-yi Yang, Zhen-lin Xu, Yu-dong Shen, Hong Wang
Summary: This study successfully selected three specific nanobodies against 19-NT and developed an ic-ELISA method using Nb2F7 with low detection limit and excellent recovery rates. The technology showed high correlation with the UPLC-MS/MS method.
Article
Engineering, Chemical
Wei-Ting Lin, Su-Chun How, Wen-Zhi Lin, Fang-Hsi Chen, Wan-Chun Liao, I-Cheng Ma, Steven S. -S. Wang, Shao-Yi Hou
Summary: A competitive assay for the detection of biotin using flow cytometry was developed. The assay showed a higher detection limit and shorter assay time compared to ELISA. It involved mixing the sample with streptavidin-display spores, fluorescein-biotin, and additional biotin for competition before analysis with a flow cytometer.
JOURNAL OF THE TAIWAN INSTITUTE OF CHEMICAL ENGINEERS
(2023)
Article
Chemistry, Analytical
Ziyi He, Justin Huffman, Kathrine Curtin, Krista L. Garner, Elizabeth C. Bowdridge, Xiaojun Li, Timothy R. Nurkiewicz, Peng Li
Summary: The cPlate system, enabled by a novel microscale fluid manipulation method, allows for highly scalable multiplexed ELISA analysis with high sensitivity and specificity, shorter assay time, and lower consumption, making it suitable for point-of-care testing in resource-limited regions.
ANALYTICAL CHEMISTRY
(2021)
Review
Chemistry, Analytical
Palaniyandi Velusamy, Chia-Hung Su, Palaniappan Ramasamy, Viswanathan Arun, Narayanan Rajnish, Pachaiappan Raman, Vaseeharan Baskaralingam, Sakkarapalayam Murugesan Senthil Kumar, Subash C. B. Gopinath
Summary: Biomarkers are biological molecules associated with physiological changes of the body, aiding in the detection and prediction of disease onset. Breath analysis, as a noninvasive, rapid, and inexpensive method, has become a research focus in early detection of health issues.
CRITICAL REVIEWS IN ANALYTICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Xuekai Zhao, Subash C. B. Gopinath, Weichao Zhao
Summary: An immunosensor based on a multiwalled carbon nanotube-modified surface was developed in this study to quantify the CRP level in blood. By constructing specific anti-CRP on the multiwalled carbon nanotube surface, the sensor exhibited a dose-dependent linear pattern in the range of 200 to 3000 pM and was able to identify CRP in biological samples.
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
(2023)
Article
Green & Sustainable Science & Technology
Muhammad Nur Aiman Uda, Subash C. B. Gopinath, Uda Hashim, Muhammad Nur Afnan Uda, Tijjani Adam, Nor Azizah Parmin, Sreeramanan Subramaniam, Suresh V. Chinni, Veeranjaneya Reddy Lebaka, Ramachawolran Gobinath
Summary: Rice is increasingly exposed to inorganic arsenic, affecting half of the world population as rice consumers. This study investigated the effects of silica and graphene nanomaterials treatment on varied arsenic levels, and found that silica nanoparticles showed the highest inhibition on arsenic accumulation. Arsenic adversely affected plant growth and even caused death at high concentrations, but silica nanoparticles demonstrated potential in reducing consumer health risks.
Review
Biotechnology & Applied Microbiology
Theivasanthi Thirugnanasambandan, Subash C. B. Gopinath
Summary: The storage of food grains against fungal infection is a major challenge for farmers, but nanotechnology offers a solution by replacing synthetic fungicides with inorganic nanoparticles such as silver and zinc oxide, as well as green synthesized nanoparticles. Plant extracts and essential oils show effective antifungal properties, and nanoparticles can be impregnated in packaging materials for safe food storage. This overview discusses the nanomaterials-mediated protection of food materials from mycotoxin contamination and its impact on the environment.
Review
Engineering, Chemical
Hussaini Adam, Subash C. B. Gopinath, M. K. Md Arshad, Tijjani Adam, Uda Hashim, Zaliman Sauli, Makram A. Fakhri, Sreeramanan Subramaniam, Yeng Chen, Sreenivasan Sasidharan, Yuan Seng Wu
Summary: This study presents an electrochemically-based microfluidic nanobiosensor array for monoplex and multiplex detection of physiologically relevant analytes. The integrated system is portable and serves as a custom electrochemical reader. It has great potential for real-time profiling of immune-mediated treatment responses and as a point-of-care diagnostic device for conducting near-patient tests using biological samples.
JOURNAL OF THE TAIWAN INSTITUTE OF CHEMICAL ENGINEERS
(2023)
Article
Biochemistry & Molecular Biology
Hussaini Adam, Subash C. B. Gopinath, Thirumananseri Kumarevel, M. K. Md Arshad, Tijjani Adam, Zaliman Sauli, Sreeramanan Subramaniam, Uda Hashim, Yeng Chen
Summary: In this study, a novel technique for the selective detection of amyloid fibrils was reported. Using a dipole moment on a dielectrode surface, this technique can sensitively detect the aggregation of alpha-synuclein, which is crucial for diagnosing Parkinson's disease. The stability and detection range of the sensor were confirmed through physical and electrical characterization.
PROCESS BIOCHEMISTRY
(2023)
Review
Green & Sustainable Science & Technology
Wan Yuen Tan, Subash C. B. Gopinath, Periasamy Anbu, Ahmad Radi Wan Yaakub, Sreeramanan Subramaniam, Yeng Chen, Sreenivasan Sasidharan
Summary: With the development of bionanotechnology, the field of nanobiocatalysts has grown rapidly and transformed various nanomaterials into novel nanocarriers for enzyme immobilization. Nanotubes, nanofibers, nanopores, nanoparticles, and nanocomposites have been successfully used as nanocarriers. The combination of enzymes and nanocarriers through different immobilization techniques has attracted attention due to their exceptional catalytic performance, stability, and reusability. The review discusses enzyme immobilization on nanocarriers and highlights the techniques, properties, preparations, and applications of nanoimmobilized enzymes.
Review
Biotechnology & Applied Microbiology
Hussaini Adam, Subash C. B. Gopinath, M. K. Md Arshad, Tijjani Adam, N. A. Parmin, Irzaman Husein, Uda Hashim
Summary: In severe cases, Parkinson's disease causes uncontrolled movements known as motor symptoms such as dystonia, rigidity, bradykinesia, and tremors. Parkinson's disease also causes non-motor symptoms such as insomnia, constipation, depression and hysteria. Disruption of neural networks in the substantia nigra pars compacta is a major cause of motor symptoms in Parkinson's disease, highlighting the need for better methods of detection. Improved diagnosis and treatment of Parkinson's disease can help avoid some of its devastating symptoms.
Review
Microbiology
Suaidah Ramli, Yuan Seng Wu, Kalaivani Batumalaie, Rhanye Mac Guad, Ker Woon Choy, Ashok Kumar, Subash C. B. Gopinath, Md. Moklesur Rahman Sarker, Vetriselvan Subramaniyan, Mahendran Sekar, Neeraj Kumar Fuloria, Shivkanya Fuloria, Suresh V. Chinni, Gobinath Ramachawolran
Summary: This review focuses on the therapeutic efficacy and molecular mechanisms of Withania somnifera (WS) extracts and their phytochemicals against SARS-CoV-2 infection. It also discusses the use of molecular docking in developing potential inhibitors from WS to target the virus and host cell receptors. Additionally, the review explores the use of nanoformulations or nanocarriers in enhancing WS delivery for improved bioavailability and treatment efficacy.
Article
Infectious Diseases
Valiappan Ranjutha, Yeng Chen, Lamya Ahmed Al-Keridis, Mitesh Patel, Nawaf Alshammari, Mohd Adnan, Sumaira Sahreen, Subash C. B. Gopinath, Sreenivasan Sasidharan
Summary: Polyalthia longifolia is a medicinal plant used in traditional medicine for skin diseases. This study confirms the synergistic antimicrobial activity of Polyalthia longifolia methanol leaf extract (MEPL) and ceftriaxone against methicillin-resistant Staphylococcus aureus (MRSA) bacteria. The combination of MEPL and ceftriaxone significantly reduces the minimum inhibitory concentration of ceftriaxone and inhibits the expression of the resistant mecA gene in MRSA.
Review
Multidisciplinary Sciences
A. L. Olatomiwa, Tijjani Adam, C. O. Edet, A. A. Adewale, Abdullah Chik, Mohammed Mohammed, Subash C. B. Gopinath, U. Hashim
Summary: This review provides a comprehensive overview of articles on the electronic and optical properties of graphene using density functional theory and machine learning approaches. It summarizes the thermodynamic stability parameters of various doped graphene systems and discusses the strengths and uncertainties of the used models and potentials. Future studies should explore the thermal properties, heterostructure modeling, superconducting behavior, and optimization of DFT models to enhance the potential of graphene.
Article
Green & Sustainable Science & Technology
Roslaili Abdul Aziz, Mok Yiwen, Mawaddah Saleh, Mohd Nazry Salleh, Subash C. B. Gopinath, Sunny Goh Eng Giap, Suresh V. Chinni, Ramachawolran Gobinath
Summary: This study evaluated the accumulation of trace elements, such as copper, zinc, and lead, in paddy fields and soil, and analyzed their transfer to rice plants. The findings show that the accumulation of heavy metals in soil varies depending on the location and the order of accumulation differs for different metals. The study also found that soil properties and metal abundance can influence the growth characteristics of rice plants.
Article
Food Science & Technology
Raja Hasnida Raja Hashim, Ahmad Anas Nagoor Gunny, Sam Sung Ting, Subash C. B. Gopinath, Yeong Yin Fong, Sunil Pareek, Muaz Mohd Zaini Makhtar, Hafiza Shukor
Summary: This study investigated the effect of Coleus aromaticus essential oil (CAEO) on an active film made of polylactic acid, chitosan, and cellulose nanocrystal for fruit packaging. The addition of CAEO improved the mechanical properties and antioxidant activity of the film, and exhibited inhibitory effects against pathogenic fungus. The results suggest that the PLA/Cs/CNC/CAEO films can enhance microbial safety and prolong the shelf life of fruits, potentially replacing petroleum-based plastics for fruit packaging applications.
JOURNAL OF FOOD MEASUREMENT AND CHARACTERIZATION
(2023)
Article
Multidisciplinary Sciences
Mohammed Mohammed, Jawad K. Oleiwi, Anwar Ja 'afar Mohamad Jawad, Aeshah M. Mohammed, Azlin F. Osman, Rozyanty Rahman, Tijjani Adam, Bashir O. Betar, Subash C. B. Gopinath, Omar S. Dahham
Summary: This study aimed to enhance the flexural properties of unsaturated polyester/kenaf fiber nanocomposites by using zinc oxide nanoparticles for surface treatment. The findings showed that the addition of ZnONPs significantly improved the performance of the composites, with 2% being the optimal loading, and surface treatment with ZnONPs resulted in kenaf fibers with improved flexural properties.
Article
Engineering, Biomedical
S. Iswarya, T. Theivasanthi, Subash C. B. Gopinath
Summary: Sodium alginate/hydroxyapatite/nanocellulose (SA/HA/NC) nanocomposite films were prepared for bone tissue engineering using a simple solution casting method. The structure and properties of the composite films were studied by XRD, SEM, EDAX, and FTIR analysis. The results showed that the inclusion of different concentrations of NC improved the tensile strength of the biopolymer film. These bionanocomposite films have the potential to be used as biomaterials in bone tissue engineering.
JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS
(2023)
