Article
Chemistry, Analytical
Xin Zhou, Hongmei Cao, Yong Zeng
Summary: In this study, a pneumatically automated microfluidic reactor device was designed to integrate analyte extraction/enrichment and DSNSA-mediated miRNA detection in a streamlined analysis workflow, achieving rapid and robust on-chip detection of miR-21 with reduced consumption of DSN enzyme. The optimized workflow demonstrated a limit-of-detection of 35 amol and has potential applications in clinical diagnosis and beyond.
Article
Nanoscience & Nanotechnology
Boxuan Ma, Hong Xu, Yanan Wang, Li Yang, Weihua Zhuang, Gaocan Li, Yunbing Wang
Summary: Atherosclerosis, a major cause of cardiovascular diseases, can be targeted and treated with newly designed nanosized drug carriers that have ROS-responsive properties, showing potential for precise theranostics in atherosclerotic lesions.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Chemistry, Analytical
Berrin Kucukturkmen, Wali Inam, Fadak Howaili, Mariam Gouda, Neeraj Prabhakar, Hongbo Zhang, Jessica M. Rosenholm
Summary: Microfluidics has been used to coat mesoporous silica nanoparticles (MSNs) with polymeric layers for achieving high protein loading and retention. This study demonstrates the potential of microfluidic technologies in the design of protein delivery systems, allowing for multicomponent systems and preservation of protein stability.
Article
Biophysics
Soochul Shin, Sun Han, Juyoung Kim, Yumi Shin, Ji-Joon Song, Sungchul Hohng
Summary: SIMUL is a multiplexed ctDNA detection technique that can sensitively and specifically detect multiple single-nucleotide mutations in early cancer stages. It accurately measures mutant fractions and holds immense potential for clinical applications.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Physical
Md Lemon Hasan, Ga Eul Kim, Mahmoud A. Elnaggar, Dae Hyeok Yang, Yoon Ki Joung
Summary: The development of neuro-specific biomaterials is aimed at overcoming the limitations of short-term usage of neural interfacing devices. The experiment demonstrated that a biomaterial covered with neuronal cell-derived membrane containing neural cell adhesion molecule (L1CAM) can promote neuronal adhesion and activation while minimizing immune responses. The neuronal activation study provided evidence that this biomaterial improves neurite outgrowth and activation stages more effectively than the commonly used poly(L-lysine) substrate.
APPLIED SURFACE SCIENCE
(2023)
Article
Biophysics
Brandon K. Ashley, Jianye Sui, Mehdi Javanmard, Umer Hassan
Summary: Researchers have developed a novel multifrequency impedance cytometry apparatus that can analyze multiple biomarkers simultaneously, improving the accuracy and personalization of disease diagnosis. By using frequency-sensitive barcoded metal oxide Janus particles as targeting agents, the apparatus can identify and classify specific receptors on neutrophils, providing more accurate information for patient prognosis.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Analytical
Yawen Liu, Xing Huang, Han Zhu, Yongkai Lu, Hongzhi Song, Junfeng Niu, Hongxia Chen
Summary: In this paper, a biomimetic probe was synthesized by functionalizing cancer cell membrane with gold nanoparticles, achieving highly sensitive detection of circulating exosomes. This strategy offers new possibilities for clinical non-invasive cancer diagnosis.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Biophysics
Ying Peng, Bing Lu, Ying Deng, Nana Yang, Genxi Li
Summary: This study proposes a dual-recognition-controlled electrochemical biosensor for the accurate detection of specific CTCs. The biosensor combines aptamer hairpin probes and rolling cycle amplification to achieve sensitive quantification of target CTCs in complex whole blood matrixes. It demonstrates excellent selectivity and stability, showing great promise in clinical cancer diagnosis and personalized medicine.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Analytical
Jamal Ahmed Cheema, Nihan Aydemir, Colm Carraher, Roshan Khadka, Damon Colbert, Harris T. Lin, Andrew Nelson, Andrew Kralicek, Jadranka Travas-Sejdic
Summary: This study presents an electrochemical sensing methodology using an insect odorant receptor (iOR) as the biological recognition element for detection of odorant compounds. The fruit fly Drosophila melanogaster's Or22a was used as a model receptor and shown to be functionally active towards its known ligand (ethyl hexanoate). The EIS sensor exhibited high sensitivity and specificity towards ethyl hexanoate with a detection limit of 5.5 fM, with neutron reflectometry studies providing evidence of conformational changes in the receptor upon ligand binding.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Biochemical Research Methods
Carole Farre, Sara Viezzi, Alice Wright, Perrine Robin, Nathalie Lejal, Marisa Manzano, Jasmina Vidic, Carole Chaix
Summary: This study developed a sensitive magnetic bead-based enzyme-linked immunoassay (MELISA) for the comprehensive detection of Influenza A virus. The assay combined immunomagnetic beads and biotin-nanoparticle-based detection to quantify the viral nucleoprotein. The use of biotin-nanoparticles enabled low detection limits and the new MELISA method showed high specificity without cross-reactivity with a porcine respiratory virus.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
Yesman Akuoko, Robert L. Hanson, David H. Harris, Jacob B. Nielsen, Elaine Lazalde, Adam T. Woolley
Summary: The study demonstrates the fabrication of microfluidic devices for DNA capture and fluorescence detection of antibiotic resistance gene sequences, showing simplicity, robustness, and affordability. Results indicate that relative fluorescence increases with target DNA concentration, with synthetic target DNA exhibiting higher fluorescence signal and percent recovery compared to plasmid DNA.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Polymer Science
Eman J. Heikal, Rashad M. Kaoud, Shadeed Gad, Hatem I. Mokhtar, Abdullah Alattar, Reem Alshaman, Sawsan A. Zaitone, Yasser M. Moustafa, Taha M. Hammady
Summary: This research developed a drug delivery system by coating alginate/chitosan beads with Eudragit (R) S-100 to target the colon and effectively treat colitis. The coated beads showed controlled release of curcumin/mesalamine at different pH levels. The optimized formula demonstrated high entrapment efficiency, swelling, and bead size. It significantly reduced colitis, indicating its potential for treating ulcerative colitis.
Article
Chemistry, Physical
Kilian Colas, Susanne Doloczki, Aikaterina Kesidou, Lourdes Sainero-Alcolado, Aida Rodriguez-Garcia, Marie Arsenian-Henriksson, Christine Dyrager
Summary: A series of solvatochromic phenylbenzothiadiazoles with dual emission properties were studied, showing sensitivity to polarity changes and potential applications in staining intracellular lipid droplets as well as fluorescence in hypoxic cancer cells. The compounds demonstrate differences in emission efficiency, spectral shifts, and ratios of locally excited and intramolecular charge transfer states.
Article
Gastroenterology & Hepatology
Qinghai Meng, Menghua Ma, Weiwei Zhang, Yunhui Bi, Peng Cheng, Xichao Yu, Yu Fu, Ying Chao, Tingting Ji, Jun Li, Qi Chen, Qichun Zhang, Yu Li, Jinjun Shan, Huimin Bian
Summary: The changes in gut microbiota in perimenopausal AS patients are associated with lipid metabolism abnormalities, and estrogen may have beneficial effects on gut bacteria and lipid metabolism.
Article
Nanoscience & Nanotechnology
Xuancheng Zhang, Wei Song, Kang Han, Zhaoyi Fang, Eunshinae Cho, Xiaoqiao Huangfu, Yaohua He, Jinzhong Zhao
Summary: A graded biomimetic scaffold coated with decellularized extracellular matrix was fabricated using 3-D bioprinting to enhance cellular differentiation and promote the regeneration of damaged tendon-to-bone enthesis. In a rabbit rotator cuff tear model, the GBS-E group exhibited superior tendon-to-bone differentiated properties and higher biomechanical properties compared to other groups at 16 weeks. This study offers a promising tissue-engineering strategy for the regeneration of complex enthesis using a 3-D bioprinting technique.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Ophthalmology
Susan P. Mollan, Dun Jack Fu, Ching-Yi Chuo, Jacqueline G. Gannon, Wen Hwa Lee, J. Jill Hopkins, Cian Hughes, Alastair K. Denniston, Pearse A. Keane, Ronald Cantrell
Summary: By combining data from a national digital health data repository with historical clinical trial data, we demonstrate an enhanced predictive modeling in nAMD. The detrimental effects of prolonged treatment delay should motivate healthcare providers to support nAMD patients in accessing care in safe environments.
BRITISH JOURNAL OF OPHTHALMOLOGY
(2023)
Article
Ophthalmology
Krishna M. Gokhale, Nicola J. Adderley, Anuradhaa Subramanian, Wen Hwa Lee, Diana Han, Jesse Coker, Tasanee Braithwaite, Alastair K. Denniston, Pearse A. Keane, Krishnarajah Nirantharakumar
Summary: This study aimed to explore the relationship between metformin use and the development of AMD in patients with type 2 diabetes in the UK. The results showed that there was no association between the use of metformin and the risk of developing AMD, regardless of whether other antidiabetic medications were also used.
BRITISH JOURNAL OF OPHTHALMOLOGY
(2023)
Article
Dentistry, Oral Surgery & Medicine
Po-Jan Kuo, Chi-Yu Lin, Tsung-Fu Hung, Hsien-Chung Chiu, Hsin-Yu Kuo
Summary: The study investigated a novel workflow integrating dynamic navigation for immediate single-implant placement in the esthetic zone. Results showed that this approach achieved successful implant placement and good aesthetics, with high patient satisfaction and no surgical or biological complications.
JOURNAL OF DENTAL SCIENCES
(2022)
Article
Pharmacology & Pharmacy
Anuradhaa Subramanian, Diana Han, Tasanee Braithwaite, Rasiah Thayakaran, Dawit T. Zemedikun, Krishna M. Gokhale, Wen Hwa Lee, Jesse Coker, Pearse A. Keane, Alastair K. Denniston, Krishnarajah Nirantharakumar, Laurent Azoulay, Nicola J. Adderley
Summary: This study aimed to assess the risk of age-related macular degeneration (AMD) in patients with hypertension using angiotensin-converting enzyme inhibitors (ACE-I) compared to calcium channel blockers (CCB). The results showed no evidence of an association between ACE-I use and the risk of AMD in hypertensive patients.
BRITISH JOURNAL OF CLINICAL PHARMACOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Tsung-Fu Hung, Po-Jan Kuo, Fung-Shi Tsai, Pin-Huan Yu, Yu-Shin Nai
Summary: This report describes the application of 3D scanning, computer-aided design (CAD), and 3D printing in making a protective device for rescuing wild animals. The integration of multiple sciences to 3D technology provides a model for veterinary medical applications. The use of new technology can save severely injured wild animals and reduce the need for intensive medication.
Article
Chemistry, Physical
Zhaobin Guo, Chih-Tsung Yang, Chia-Chi Chien, Luke A. Selth, Pierre O. Bagnaninchi, Benjamin Thierry
Summary: Measuring the optical cellular micromotion can accurately determine the invasive behavior of single tumor cells within tissues, providing a new tool for studying tumor invasion mechanisms and identifying therapeutic targets.
Review
Ophthalmology
Alastair K. Denniston, Aditya U. Kale, Wen Hwa Lee, Susan P. Mollan, Pearse A. Keane
Summary: This review discusses the challenges of creating a trusted resource for real-world data in ophthalmology, using the UK's INSIGHT Health Data Research Hub as an example. INSIGHT utilizes historical patient-level electronic health record data, including images, to enable research and maximize patient benefit. The establishment of patient and public trust, efficient governance processes, and the potential for discovering ophthalmic biomarkers of systemic diseases are highlighted.
CURRENT OPINION IN OPHTHALMOLOGY
(2022)
Article
Chemistry, Analytical
Jinxiu Hou, Yi Xu, Shasha Sun, Xiaoli Zhong, Chih-Tsung Yang, Xin Zhou
Summary: Label-free SPR is rarely used for biomarker detection in real samples due to its low sensitivity. Here, a phage-based SPR (P-SPR) strategy for the detection of carcinoembryonic antigen (CEA) in undiluted serums is reported. The proposed P-SPR method demonstrated ultrasensitive detection of CEA within 10 min, with a detection limit of 0.83 fM. The strategy also allows for cross-validation of SPR results through naked-eye counting of the plaques generated by the P-SPR probes, enabling a reliable dual detection system. The P-SPR scheme shows excellent sensing performance for the detection of real samples at concentrations lower than the LOD of ELISA.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Xuejia Qian, Yuanzhao Shen, Jiasheng Yuan, Chih-Tsung Yang, Xin Zhou
Summary: In this study, a method combining gold nanorod probes and dark field microscopy was proposed for rapid and quantitative detection of porcine epidemic diarrhea virus. The method showed strong specificity, good repeatability, and low detection limit, making it applicable for rapid on-site detection of pathogens.
Article
Oncology
Ting -Fang Che, Chao-Hua Chiu, Yu-Chung Wu, Jia-Yang Chen, Teh-Ying Chou, Yi-Chun Cheng, Chi-Lu Chiang, Chien-Sheng Huang, I-Shuan Tuang, Yang-Hui Ho, Jian-Hua Hong, Yen-Jang Huang, Hsiang-Ling Ho, Ying-Chih Chang
Summary: We evaluated the clinical significance of circulating tumor cells (CTCs) in lung adenocarcinoma (LUAD) by combining efficient CTC isolation and in-vitro cultivation. The cultured CTC count and proliferative ability were highly associated with cancer prognosis, indicating their potential as prognostic biomarkers in LUAD patients.
Review
Chemistry, Multidisciplinary
Yuanzhao Shen, Jingyu Wang, Yuting Li, Chih-Tsung Yang, Xin Zhou
Summary: Malignant tumor is a major cause of human death. Recently, genetically engineered bacteriophages have been used as probes for detecting highly expressed antigens in tumor cells and as anti-tumor agents. Moreover, phages can be chemically modified and assembled with nanoparticles to expand their applications in biological detection and tumor therapy. This review summarizes the studies on genetically engineered and chemically modified phages in the diagnosis and targeting therapy of tumors, discusses their advantages and limitations, and proposes suitable application scenarios.
Article
Oncology
Ting-Yu Chiang, Hung-Chih Hsu, Yih-Jong Chern, Chun-Kai Liao, Yu-Jen Hsu, Wen-Sy Tsai, Pao-Shiu Hsieh, Yu-Fen Lin, Hsiu-Lan Lee, Jeng-Fu You
Summary: The primary treatment for metastatic colorectal cancer (mCRC) includes targeted therapy and chemotherapy. Anti-EGFR antagonists can cause skin reactions in 50-70% of patients. Severe skin reactions may be linked to overall survival and progression-free survival, but the results are uncertain. A retrospective analysis of mCRC patients undergoing anti-EGFR therapy showed that the first-line treatment significantly affected overall survival and progression-free survival. There was a gender difference in the incidence of acne caused by anti-EGFR antagonists.
Article
Biophysics
Jinxiu Hou, Xuejia Qian, Yi Xu, Zhirui Guo, Benjamin Thierry, Chih-Tsung Yang, Xin Zhou, Chuanbin Mao
Summary: A rapid and ultrasensitive optical biosensing scheme was developed to detect avian influenza virus H9N2. The scheme used a genetically engineered filamentous M13 phage probe to enhance the detection signal. The phage-based strategy could detect H9N2 viruses in real samples within 10 minutes, even at low concentrations.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Surgery
Kun-Yu Tsai, Jeng-Fu You, Shu-Huan Huang, Tzong-Yun Tsai, Pao-Shiu Hsieh, Cheng-Chou Lai, Wen-Sy Tsai, Hsin-Yuan Hung
Summary: This study aimed to investigate the influence of the timing of stoma closure on clinical and oncological outcomes for rectal cancer patients. The results showed that complications occurred more frequently after stoma closure, while complications during stoma closure had no significant impact on long-term survival.
LANGENBECKS ARCHIVES OF SURGERY
(2023)
Article
Materials Science, Biomaterials
Po-Ying Yeh, Jia-Yang Chen, Mo-Yuan Shen, Ting-Fang Che, Syer Choon Lim, Jocelyn Wang, Wen-Sy Tsai, Curtis W. Frank, Chun-Jen Huang, Ying-Chih Chang
Summary: Due to the scarcity, vulnerability, and heterogeneity of circulating tumor cells (CTCs), the existing CTC isolation system based on immunoaffinity interaction lacks consistent efficiency. The newly developed LIPO-SLB platform, incorporating a chaotic-mixing microfluidic system and antibody-conjugated liposome-tethered-supported lipid bilayer, offers high CTC capture efficiency, viability, and selectivity. The platform has been successfully validated using different cancer cell lines and clinical samples, demonstrating its enormous potential for prognostic and predictive purposes in precision medicine.
JOURNAL OF MATERIALS CHEMISTRY B
(2023)