Article
Cell Biology
Dhirendra Pratap Singh, Rizwana Begum, Gagandeep Kaur, Prathyusha Bagam, Devaiah Kambiranda, Rakesh Singh, Sanjay Batra
Summary: Electronic cigarettes (e-cigs) are popular battery-operated heating devices that are marketed as a safer alternative to traditional cigarettes. However, recent reports of lung injuries associated with e-cig use have sparked a debate on the potential harms of vaping. Researchers are increasing their efforts to understand the effects of vaping on human health, including the inflammatory response and pulmonary health issues caused by e-cigs.
CELL BIOLOGY AND TOXICOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Mohammed F. Gholam, Lauren P. Liu, Louis A. Searcy, Nancy D. Denslow, Abdel A. Alli
Summary: The SGLT2 inhibitor dapagliflozin may improve comorbidities associated with type 2 diabetes mellitus by altering lipid composition, suppressing extracellular vesicle release, and disrupting lipid rafts in the kidney.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Hematology
Tiphaine Belleville-Rolland, Alexandre Leuci, Alexandre Mansour, Benoit Decouture, Fanny Martin, Sonia Poirault-Chassac, Margot Rouaud, Hippolyte Guerineau, Blandine Dizier, Dominique Pidard, Pascale Gaussem, Christilla Bachelot-Loza
Summary: The study found that a small fraction of MRP4 and most inhibitory G-protein Gi were located in lipid rafts; the integrity of lipid rafts is necessary for cAMP signaling regulation, even though MRP4 and most cAMP homeostasis proteins are mainly located outside rafts.
THROMBOSIS AND HAEMOSTASIS
(2021)
Article
Biochemistry & Molecular Biology
Gaetan Chicanne, Maria N. Barrachina, Anaelle Durbec, Justine Bertrand-Michel, Sara Troitino, Lidia Hermida-Nogueira, Aurelio M. Sueiro, Maria Pardo, Bernard Payrastre, Angel Garcia
Summary: The goal of this study was to compare the lipid profile of platelets from non-diabetic patients with severely obese patients. The results showed that platelets from obese patients had a unique lipid fingerprint, which may be related to the hyperactive state of platelets in obesity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Medicine, Research & Experimental
Xu Wang, Yanhua Gao, Jia Zhang, Huaxing Zhang, Shuo Sun, Suwen Su, Dezhi Kong, Qiao Wang
Summary: This study identified the components of Lingguizhugan Decoction (LGZGD) using the UHPLC-QTOF-MS method and found that LGZGD regulates lipid metabolism. Serum lipidomics and heart tissue proteomics revealed the effects of LGZGD on lipid metabolism. The Prakg2/Ucp2/Plin1 axis on the Apelin pathway was found to play a vital role in the cardioprotective effects of LGZGD.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Microbiology
Daniel Zamith-Miranda, Heino M. Heyman, Meagan C. Burnet, Sneha P. Couvillion, Xueyun Zheng, Nathalie Munoz, William C. Nelson, Jennifer E. Kyle, Erika M. Zink, Karl K. Weitz, Kent J. Bloodsworth, Geremy Clair, Jeremy D. Zucker, Jeremy R. Teuton, Samuel H. Payne, Young-Mo Kim, Morayma Reyes Gil, Erin S. Baker, Erin L. Bredeweg, Joshua D. Nosanchuk, Ernesto S. Nakayasu
Summary: This study built a comprehensive map of the Histoplasma capsulatum lipid metabolic pathway by incorporating proteomic and lipidomic analyses. The map identified fatty acid desaturation and sphingolipid biosynthesis pathways as targets for drug development. Experimental results showed inhibitors of these pathways effectively inhibited H. capsulatum growth.
Article
Biotechnology & Applied Microbiology
Kohei Yoneda, Yohei Ishibashi, Masaki Yoshida, Makoto M. Watanabe, Makoto Ito, Iwane Suzuki
Summary: This study analyzed the proteins and lipids in the lipid droplets of Aurantiochytrium limacinum. The results showed the presence of specific lipid droplet proteins, as well as proteins commonly found in lipid droplets from other organisms. The lipid analysis revealed the presence of unsaturated triacylglycerols and phytosterol esters in the lipid droplets.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2022)
Article
Food Science & Technology
Yu-Yan Wang, Shi-Juan Lu, Rui Gui, Jian-Ping Wu, Jing Li, Xiao-Ai He, Wei Zhang, Gui-Ming Deng, Wen-Xuan Wang, Hong-Ping Long, Xi-Fan Wei, Guang-Yao Zeng, Na Zhang, Shu-Min Zang, Ye Yao, Zu-Hui Chen, Cheng Fei, Yi-Kun Wang, Kang-Ping Xu
Summary: In this study, the efficacy and related mechanisms of Cyclocarya paliurus flavonoids (CPF) in preventing non-alcoholic steatohepatitis (NASH) were investigated using lipidomics and proteomics. CPF supplementation significantly reduced body and liver weight gain, cholesterol and triacylglycerols (TGs) levels in NASH mice, and improved hepatic steatosis by reducing liver inflammation score and the area of liver fibrosis. Lipidomics analysis showed that the prevention of NASH by CPF was closely associated with the decrease in levels of TGs, diacylglycerols (DGs), and ceramides (Cers). Proteomics analysis revealed that CPF promoted metabolic homeostasis in the liver. The study concluded that the remodulation of lipid homeostasis may be a pivotal pathway for the potential mechanisms of CPF in protecting against NASH.
JOURNAL OF FUNCTIONAL FOODS
(2022)
Article
Biochemistry & Molecular Biology
Karel Harant, Tomas Cajka, Pavla Angelisova, Jana Pokorna, Vaclav Horejsi
Summary: English Summary: The styrene-maleic acid (SMA) amphipathic copolymers have emerged as a beneficial alternative to detergents for studying membrane proteins. In our recent study, we found that most T cell membrane proteins were soluble in small nanodiscs using this method, while raft proteins, specifically GPI-anchored proteins and Src family kinases, were present in larger membrane fragments enriched in raft lipids. Our present study further confirms this pattern of membrane disintegration by SMA copolymer in other cell types and provides a detailed characterization of these SMA-resistant membrane fragments (SRMs) at the proteomic and lipidomic levels.
BIOPHYSICAL CHEMISTRY
(2023)
Article
Biology
Isin Tuna Sakallioglu, Bridget Tripp, Jacy Kubik, Carol A. Casey, Paul Thomes, Robert Powers
Summary: Excessive alcohol consumption causes the greatest damage to the liver due to its detoxification primarily taking place in the liver. Fatty liver is one of the initial pathological changes that occur in response to alcohol consumption. Continuous alcohol abuse can lead to more severe liver diseases such as fibrosis, cirrhosis, and cancer. This study utilized high-throughput biochemical assays and multiomics analysis to identify molecular changes induced by alcohol during the development of fatty liver. The findings revealed alterations in fatty acid species, glucuronidation, and cholesterol metabolism, providing insights into potential targets for treating alcohol related fatty liver disease.
Article
Multidisciplinary Sciences
Rotimi Fasimoye, Wentao Dong, Raja S. Nirujogi, Eshaan S. Rawat, Miharu Iguchi, Kwamina Nyame, Toan K. Phung, Enrico Bagnoli, Alan R. Prescott, Dario R. Alessi, Monther Abu-Remaileh
Summary: The Golgi is a crucial organelle involved in protein and lipid biosynthesis, as well as sorting and secretion. Dysfunctional Golgi is associated with various diseases. This study presents a Golgi immunoprecipitation technique to isolate intact Golgi mini-stacks for analysis. The analysis pipeline reveals the molecular features of the human Golgi, including proteome, metabolome, and lipidome profiles. This comprehensive molecular map provides a powerful method to study the Golgi in health and disease.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Virology
Naoyuki Iida, Madoka Kawahara, Riku Hirota, Yoshio Shibagaki, Seisuke Hattori, Yuko Morikawa
Summary: The cell-cell contact between HIV-1-infected and uninfected cells forms a virological synapse (VS) to allow for efficient HIV-1 transmission. Mass spectrometry revealed the recruitment of various molecules, including ATP-related enzymes, protein translation factors, and charged multivesicular body protein 4B, to the VS. Further exploration showed that vimentin plays a role in HIV-1 transmission through the recruitment of CD4 to the cell-cell interface.
Article
Food Science & Technology
Wei Jia, Xixuan Wu, Lin Shi
Summary: This study investigated the effects of dietary hydrocortisone intake on lipid metabolism. The results showed that hydrocortisone promoted triglyceride hydrolysis and decreased the concentration of certain fatty acids. Additionally, a significant increase in saturated triglycerides concentration over time was observed.
MOLECULAR NUTRITION & FOOD RESEARCH
(2022)
Article
Chemistry, Physical
Sheeba Malik, Smarajit Karmakar, Ananya Debnath
Summary: All-atom molecular dynamics simulations reveal a significant growth in the heterogeneity length scales of interface water (IW) in 1,2-dimyristoyl-sn-glycero-3-phosphocholine lipid membranes across fluid to ripple to gel phase transitions. IW acts as an alternate probe to capture the ripple size of the membrane and follows an activated dynamical scaling within the gel phase. These results quantify the mostly unknown correlations between the spatiotemporal scales of IW and membranes at various phases under physiological and supercooled conditions.
JOURNAL OF CHEMICAL PHYSICS
(2023)
Article
Engineering, Environmental
Chao Liu, Xuemeng Zhang, Chuang Chen, Yue Yin, Guohua Zhao, Yinguang Chen
Summary: The dysfunction of acetotrophic methanogens' lipids under high ammonia pressure in anaerobic digestion was investigated. High ammonia exposure significantly reduced the content of polyunsaturated and longer chain lipids, while increasing the content of lipids with shorter chains and/or higher saturation. The study also revealed the impact of ammonia on the structure of extracellular polymeric substances (EPSs) and the expression of functional proteins involved in EPS building blocks' supply.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)