Article
Plant Sciences
Shuai Hou, Fangkun Wu, Zhiqiang Wang, Ning Yan, Hao Chen, Haojie Li, Peiyu Yang, Ying Zhang, Caixia Li, Yu Lin, Jian Ma, Lin Huang, Yaxi Liu
Summary: Stripe rust is a destructive disease that significantly reduces wheat yields globally. To control this disease, we developed a resistant cultivar called 'N2496', which showed high resistance and could be used in breeding programs. Through crossing 'N2496' with common wheat cultivars, we identified a quantitative trait locus (QTL) for stripe rust resistance on chromosome arm 6BL. The QTL explained a high percentage of phenotypic variation and was validated in other populations.
Article
Plant Sciences
Rujia Zhang, Yiming Ren, Huiyuan Wu, Yu Yang, Mengguo Yuan, Haonan Liang, Changwei Zhang
Summary: In this study, two varieties of Chinese cabbage were crossed to develop segregating populations with trichome or glabrous leaves. Bulked segregant analysis identified three candidate genes that may participate in leaf trichome formation. This study demonstrates the potential of BSA in mapping genes associated with traits and provides insights into the molecular mechanism of leafy trichome formation in Chinese cabbage.
Article
Plant Sciences
Caixia Gan, Chenghuan Yan, Wenxing Pang, Lei Cui, Pengyu Fu, Xiaoqing Yu, Zhengming Qiu, Meiyu Zhu, Zhongyun Piao, Xiaohui Deng
Summary: This study reveals the inheritance and molecular mechanisms of clubroot resistance in radish, identifying a novel locus responsible for resistance and obtaining core breeding materials for resistance breeding using marker-assisted selection technology.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Wei Guan, Junhan Shan, Mingyang Gao, Jianping Guo, Di Wu, Qian Zhang, Jing Wang, Rongzhi Chen, Bo Du, Lili Zhu, Guangcun He
Summary: The study utilized BSR-seq technology to identify DEGs and genetic loci associated with BPH virulence, revealing that genes related to metabolism, endocrine system, and signal transduction were upregulated in avirulent insects feeding on resistant rice, while shifts in lipid metabolism and digestive system pathways were crucial for virulent BPHs' adaptation to resistant rice.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Wenqi Ding, Yunli Wang, Cong Qi, Yusong Luo, Chaojie Wang, Wenlong Xu, Shuping Qu
Summary: Dwarfism is an important trait in pumpkin that affects yield. The dwarf Cucurbita pepo L. line X10 showed shorter cell length in the stem and was sensitive to gibberellin biosynthesis. Genetic analysis revealed that the CpDw locus controls this dwarf trait, with Cp4.1LG10g05910.1 identified as a candidate gene for CpDw.
Article
Plant Sciences
Huangkai Zhou, Kuanqiang Tang, Guang Li, Wenqiang Liu, Hui Yu, Xiaohui Yuan, Suxin Yang, Madan K. Bhattacharyya, Xianzhong Feng
Summary: The M2-seq method enables efficient and rapid mapping of candidate mutant genes in the M-2 generation, removing background variations and aiding in the identification of causal mutations.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Agronomy
Jiajun Liu, Peng Gao, Xuezheng Wang, Hongyu Liu, Shuangwu Ma, Jiming Wang, Feishi Luan
Summary: Internode length is an important characteristic of watermelon plant architecture. This study identified a candidate gene for regulating watermelon dwarfism through genetic analysis and gene identification.
Article
Agronomy
Zhiyuan Liu, Tiantian Lu, Chunda Feng, Helong Zhang, Zhaosheng Xu, James C. Correll, Wei Qian
Summary: The study successfully fine mapped the gene controlling fruit spines in spinach to the Fs gene, and developed molecular markers for marker-assisted selection. The spiny trait in spinach fruit is controlled by a single dominant gene Fs. Through super-BSA method and recombinants analysis, this gene was mapped to a 1.9-Mb interval on chromosome 3.
THEORETICAL AND APPLIED GENETICS
(2021)
Article
Agronomy
Junying Zhang, Jifeng Zhu, Liyong Yang, Yanli Li, Weirong Wang, Xirong Zhou, Jianxia Jiang
Summary: In this study, the formation of plant cuticular wax and its genetic control in Brassica napus L. were investigated. BSA and genome-wide resequencing identified a genomic region and SNPs associated with the wax powder trait. qRT-PCR analysis identified three candidate genes showing differential expression between waxy and waxless parents. GO and KEGG pathway analysis revealed the functional enrichment of one gene in lipid biosynthesis or metabolism.
Article
Plant Sciences
Yunfeng Jiang, Luyao Duan, Fangnian Guan, Fangjie Yao, Li Long, Yuqi Wang, Xuyang Zhao, Hao Li, Wei Li, Qiang Xu, Qiantao Jiang, Jirui Wang, Yuming Wei, Jian Ma, Houyang Kang, Pengfei Qi, Mei Deng, Youliang Zheng, Guoyue Chen
Summary: In this study, a candidate genomic region strongly associated with stripe rust resistance on chromosome 1AL was identified using bulked segregant analysis coupled with exome capture sequencing (BSE-seq). The gene, designated as YrXH-1AL, was validated by a conventional quantitative trait locus analysis and explained up to 48.50% of the phenotypic variance. Two cosegregating KASP markers showed high polymorphisms among Chinese wheat cultivars and could be used for marker-assisted selection.
Article
Plant Sciences
Yijing Gao, Shan Zhou, Yuxin Huang, Baoqing Zhang, Yuhui Xu, Gemin Zhang, Prakash Lakshmanan, Rongzhong Yang, Hui Zhou, Dongliang Huang, Junxian Liu, Hongwei Tan, Weizhong He, Cuifang Yang, Weixing Duan
Summary: In this study, high-throughput genotyping methods, SLAF-seq and BSR-seq, were used to identify 21 major QTLs and 4 candidate resistance genes associated with smut resistance in sugarcane. Comparative analysis of major QTLs and BSR-seq data with the genome of the sugarcane progenitor identified several co-localized markers, providing practical tools for marker-assisted smut resistance breeding in sugarcane.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biotechnology & Applied Microbiology
Qiang Wang, Yi Wu, Anqi Peng, Jilai Cui, Mingyue Zhao, Yuting Pan, Mengting Zhang, Kai Tian, Wilfried Schwab, Chuankui Song
Summary: The tea plant is an economically important woody beverage crop. This study established a fast protoplast isolation method and investigated the transcriptional profiles of 16,977 single cells from 1st and 3rd leaves. By identifying marker genes, constructing a transcriptome atlas, and mapping developmental trajectories, the study revealed the distribution of different cell types during leaf differentiation and genes associated with cell fate transformation. A novel metabolism pathway of catechin esters in plants was also discovered through gene co-expression network analysis.
PLANT BIOTECHNOLOGY JOURNAL
(2022)
Article
Plant Sciences
Dawei Ma, Hao Tang, Michael Reichelt, Eerik-Mikael Piirtola, Juha-Pekka Salminen, Jonathan Gershenzon, C. Peter Constabel
Summary: Flavonoids are important secondary metabolites in plants, regulated by MYB transcription factors. The study identified that the MYB activator MYB117 can enhance anthocyanin accumulation in hybrid poplar by up-regulating F3'5'H1, resulting in increased B-ring hydroxylation.
JOURNAL OF EXPERIMENTAL BOTANY
(2021)
Article
Agronomy
Yahui Li, Ruiming Lin, Jinghuang Hu, Xiaohan Shi, Dan Qiu, Peipei Wu, Gebremedhin Habteab Goitom, Siqi Wang, Hongjun Zhang, Li Yang, Hongwei Liu, Qiuhong Wu, Jingzhong Xie, Yang Zhou, Zhiyong Liu, Hongjie Li
Summary: A new stripe rust resistance gene called Yr041133 was identified in winter wheat line 041133, which exhibited resistance to multiple pathotypes of the disease.
Article
Agronomy
Renhui Zhao, Bingliang Liu, Wentao Wan, Zhengning Jiang, Tiantian Chen, Ling Wang, Tongde Bie
Summary: A novel adult-plant leaf rust resistance gene, LrYang16G216, was identified and mapped to a 0.59 cM genetic interval on wheat chromosome 6BL using BSA and conventional linkage mapping. This discovery is important for developing disease-resistant wheat cultivars, as leaf rust is one of the most devastating fungal diseases of wheat.
THEORETICAL AND APPLIED GENETICS
(2023)
Letter
Agriculture, Multidisciplinary
Chen-Kai Jiang, Liang Chen
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2020)
Article
Plant Sciences
Chen-Kai Jiang, Jian-Qiang Ma, Yu-Fei Liu, Jie-Dan Chen, De-Jiang Ni, Liang Chen
HORTICULTURE RESEARCH
(2020)
Article
Multidisciplinary Sciences
Songlin Wang, Jiedan Chen, Jianqiang Ma, Jiqiang Jin, Liang Chen, Mingzhe Yao
SCIENTIFIC REPORTS
(2020)
Article
Plant Sciences
Jie-Dan Chen, Chao Zheng, Jian-Qiang Ma, Chen-Kai Jiang, Sezai Ercisli, Ming-Zhe Yao, Liang Chen
HORTICULTURE RESEARCH
(2020)
Article
Agriculture, Multidisciplinary
Jun-Ya Wang, Jie-Dan Chen, Song-Lin Wang, Liang Chen, Chun-Lei Ma, Ming-Zhe Yao
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2020)
Article
Agriculture, Multidisciplinary
Chao Zheng, Jian-Qiang Ma, Chun-Lei Ma, Ming-Zhe Yao, Jie-Dan Chen, Liang Chen
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2020)
Article
Multidisciplinary Sciences
Honglian Gu, Yu Wang, Hui Xie, Chen Qiu, Shuning Zhang, Jun Xiao, Hongyan Li, Liang Chen, Xinghui Li, Zhaotang Ding
SCIENTIFIC REPORTS
(2020)
Article
Plant Sciences
Qingxue Guo, Jianhui Ye, Jianming Zeng, Liang Chen, Helena Korpelainen, Chunyang Li
Summary: Selenium acquisition from daily diet can reduce disease risk. The main source of dietary Se is the edible parts of crop plants, which is determined by Se bioavailability in soil. Recent research has focused on the biogeochemical cycle of Se driven by specific microorganisms, emphasizing the oxidizing process. Plant root exudates and rhizosphere microorganisms affect soil Se availability. Beneficial microorganisms, including endophytes, promote crop quality and improve crop tolerance to stresses. Understanding Se transformation along the plant-soil continuum is crucial for agriculture and human health.
HORTICULTURE RESEARCH
(2023)
Article
Plant Sciences
Yi Wang, Yu-Fei Liu, Meng-Yuan Wei, Chen-Yu Zhang, Jie-Dan Chen, Ming-Zhe Yao, Liang Chen, Ji-Qiang Jin
Summary: Caffeine is a functional component in tea that can cause excitement and nerve stimulation, but excessive intake can lead to insomnia and dysphoria. Developing tea with low-caffeine content can meet the needs of certain individuals.
HORTICULTURE RESEARCH
(2023)
Article
Chemistry, Applied
Dingding Liu, Kang Wei, Chenyu Zhang, Haoran Liu, Yang Gong, Yuanyuan Ye, Jiedan Chen, Mingzhe Yao, Liang Chen, Chunlei Ma
Summary: Research has found that albino tea, with its high amino acid content, has attracted attention in the tea market due to its attractive appearance and fresh taste. The study showed that the main amino acid content gradually decreased as the tea plant aged. Principal component analysis revealed that the amino acid content of individual plants became stable with plant growth. Correlation analysis indicated a significant negative correlation between several main amino acids and the contents of chlorophyll a, chlorophyll b, and carotenoids. The results of the study suggest that the accumulation of amino acids in tea plants is closely related to leaf color variation and tree age during the growing period.
Article
Biochemistry & Molecular Biology
Yuanyuan Li, Chenyu Zhang, Chunlei Ma, Liang Chen, Mingzhe Yao
Summary: In this study, tea leaf-color mutants were studied using transcriptome and biochemical analysis, and it was found that the reduced chlorophyll content might be caused by the activation of genes related to chlorophyll degradation, along with a reduction in flavonoid biosynthesis and an accumulation of glutamate. These findings provide new insights into the molecular mechanisms underlying leaf-color bud mutation in tea plants.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Chenyu Zhang, Haoran Liu, Junya Wang, Yuanyuan Li, Dingding Liu, Yuanyuan Ye, Rong Huang, Sujuan Li, Liang Chen, Jiedan Chen, Mingzhe Yao, Chunlei Ma
Summary: This study identifies a major-effect quantitative trait locus (QTL) responsible for chlorosis in tea leaves and reveals the relationship between genetic mutations, chlorophyll biosynthesis, and amino acid metabolism.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Agronomy
Chen-Kai Jiang, De-Jiang Ni, Ming-Zhe Yao, Jian-Qiang Ma, Liang Chen
Summary: Metabolites, especially flavonoids, amino acids, organic acids, and alkaloids, showed distinct seasonal variations in fresh tea shoots of different tea accessions. Key genes and downstream compounds in the flavonoid pathway were lowest in spring, but catechin quality index was highest in this season. Regulatory pathways were explored through metabolite correlation network and weighted gene co-expression network.
FRONTIERS OF AGRICULTURAL SCIENCE AND ENGINEERING
(2021)