Article
Horticulture
Jun Zhang, Hui Xia, Dong Liang, Lijin Lin, Honghong Deng, Xiulan Lv, Zhihui Wang, Xiao'ai Zhang, Jin Wang, Bo Xiong
Summary: Dehydrins (DHNs) play a vital role in enhancing plant tolerance to abiotic stresses. In kiwifruit, seven AcDHNs were identified, showing potential involvement in leaf or fruit development. Furthermore, under various stress conditions, some of these genes showed significantly increased transcription levels, providing a foundation for further functional studies.
SCIENTIA HORTICULTURAE
(2021)
Article
Horticulture
Taghleb Al-Deeb, Mohammad Abo Gamar, Najib El-Assi, Hmoud Al-Debei, Rabea Al-Sayaydeh, Ayed M. Al-Abdallat
Summary: The study identified an orthologous gene of the DWARF AND DELAYED FLOWERING (DDF) members in Arabidopsis, SlDDF2, in tomato plants and observed its clear induction in response to ABA treatment, cold, salinity, and drought stresses. Transgenic lines with stress-inducible overexpression of SlDDF2 showed growth retardation phenotypes and higher chlorophyll content under stress conditions. Compared to wild-type plants, the transgenic lines exhibited enhanced tolerance to different abiotic stresses including water deficit, salinity, and cold.
Article
Biotechnology & Applied Microbiology
Preshobha Kodackattumannil, Kenna Whitley, Shina Sasi, Geetha Lekshmi, Saranya Krishnan, Salima Al Senaani, Martin Kottackal, Khaled M. A. Amiri
Summary: A novel inducible promoter DREB1G from the Date palm was isolated and characterized, exhibiting higher expression levels compared to the widely used AtRD29A promoter under drought and salinity stress. It can be used to develop stress-tolerant transgenic plants by inducing the expression of stress-inducible genes.
PLANT CELL TISSUE AND ORGAN CULTURE
(2023)
Article
Biochemistry & Molecular Biology
Siwar Ghanmi, Steffen P. Graether, Moez Hanin
Summary: In this study, the conservation of halophytic dehydrin sequences was analyzed, and new segments were discovered. Modifications were found in the halophytic dehydrins compared to orthologs in glycophytic plants. Furthermore, it was observed that dehydrins are upregulated by salt stress, and the intensity of this upregulation depends on the dehydrin architecture.
Article
Plant Sciences
Bartosz M. Szabala
Summary: Dehydrins are stress-induced proteins that protect cellular machinery from the adverse effects of dehydration. This study investigated the biochemical and structural properties of the dehydrin DHN24. It was found that overexpression of DHN24 inhibited bacterial growth and purified DHN24 protected lactate dehydrogenase from freeze-induced denaturation. Furthermore, DHN24 exhibited different structural conformations in aqueous solutions and membrane-mimetic environments.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Siwar Ghanmi, Margaret A. Smith, Ikram Zaidi, Marwa Drira, Steffen P. Graether, Moez Hanin
Summary: In this study, a FSK2-type dehydrin named AhDHN1 was isolated and characterized from the halophytic plant Atriplex. AhDHN1 was found to be induced by salt and water stress treatments in Atriplex seedling leaves. Circular dichroism spectrum analysis showed that AhDHN1 lacked secondary structure but gained α-helicity in the presence of SDS micelles. In vitro assays demonstrated that AhDHN1 effectively protected the enzymatic activity of lactate dehydrogenase against cold, heat, and dehydration stresses. These findings suggest that AhDHN1 may be involved in the adaptation mechanisms of halophytes to adverse environments.
Article
Biochemistry & Molecular Biology
Xiaoyu Wang, Hao Liu, Zhengyang Yu, Weining Zhu, Linsheng Zhang, Bo Wang
Summary: This study isolated the full-length and promoter sequences of Wrab18 from the Zhengyin 1 cultivar of Triticum aestivum and analyzed their characteristics. The results showed that the promoter sequence of Wrab18 contains multiple stress-related cis-acting elements and can be expressed in Nicotiana benthamiana. Moreover, gene expression levels were found to respond to stress factors. This study provides insights into the role of WRAB18 in plant stress responses and lays the foundation for further research on gene function and wheat quality improvement.
MOLECULAR BIOLOGY REPORTS
(2023)
Article
Plant Sciences
Marwa Harbaoui, Walid Ben Romdhane, Anis Ben Hsouna, Faical Brini, Rania Ben Saad
Summary: The gene TdAnn6, encoding an annexin protein in durum wheat, plays an important role in plant stress response. Expressing TdAnn6 in Arabidopsis improved stress tolerance and showed enhanced promoter activity under various stress conditions, indicating a potential regulatory role in crop responses to salt and osmotic stress.
Article
Agronomy
Amandeep Kaur, Alok Madhu, Alok Sharma, Kashmir Singh, Santosh Kumar Upadhyay
Summary: This study identified three Piezo genes in the Triticum aestivum genome and found that they are involved in plant growth and development and response to various stresses, potentially related to calcium ion signaling.
Review
Biochemistry & Molecular Biology
Zhenping Sun, Shiyuan Li, Wenyu Chen, Jieqiong Zhang, Lixiao Zhang, Wei Sun, Zenglan Wang
Summary: Dehydrins, also known as Group II late embryogenesis abundant (LEA) proteins, are highly hydrophilic classic intrinsically disordered proteins that play a protective role in plants exposed to abiotic stress. They are involved in seed maturation, plant stress tolerance, and potentially in stabilizing DNA conformations and protecting membranes and proteins. The regulatory networks of dehydrin gene expression, including ABA, MAP kinase cascade, and Ca2+ signaling pathways, form a complex, diverse system that may regulate the same dehydrin.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Agronomy
Dominika Radzikowska-Kujawska, Paula John, Tomasz Piechota, Marcin Nowicki, Przemyslaw Lukasz Kowalczewski
Summary: In order to prevent further degradation of the environment, restrictions on the use of plant protection products and fertilizers are being strengthened annually. This study evaluated the effectiveness of four commercial biostimulants in improving the tolerance of winter wheat to drought stress. Among the biostimulants tested, those based on bacterial strains had the strongest positive effects on wheat plants' drought tolerance. These treatments led to higher CO2 assimilation, reduced transpiration values, and increased stomatal conductance under drought conditions, indicating improved water use efficiency. The use of these bacterial biostimulants also resulted in less damage to the photosynthetic apparatus and higher grain yield compared to other treatments. These findings suggest the potential of these biostimulants in mitigating drought-related yield losses in wheat.
Article
Plant Sciences
Marcia Barquero, Jorge Poveda, Ana M. Laureano-Marin, Noemi Ortiz-Liebana, Javier Branas, Fernando Gonzalez-Andres
Summary: This study analyzed the mechanisms by which Rhizobium leguminosarum strains alleviate drought stress in wheat plants. The results showed that two strains (LBM1210 and LET4910) significantly improved the growth parameters and chlorophyll content of the plants. They also reduced the levels of MDA, H2O2, and ABA. Furthermore, the two strains regulated the expression of different genes involved in drought stress response, while ethylene metabolism did not seem to be involved.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Samantha Higgins, Valeryia Serada, Benjamin Herron, Kiran R. Gadhave, Dmitry Kurouski
Summary: This study investigates the accuracy of Raman spectroscopy in diagnosing both biotic and abiotic stresses in wheat. The results demonstrate that a hand-held Raman spectrometer can provide an efficient, scalable, and accurate diagnosis of these stresses in the field.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Multidisciplinary Sciences
Xiangchi Zhang, Chao Li, Weidan Lu, Xiaoli Wang, Bin Ma, Kaiyong Fu, Chunyan Li, Cheng Li
Summary: The study found that drought stress on wheat can be mitigated more effectively under low phosphorus conditions, and the regulatory genes differ between the two varieties. Xindong20 exhibits higher drought tolerance, with more up-regulated genes involved in the response.
Review
Biochemistry & Molecular Biology
Hude Mao, Cong Jiang, Chunlei Tang, Xiaojun Nie, Linying Du, Yuling Liu, Peng Cheng, Yunfeng Wu, Huiquan Liu, Zhensheng Kang, Xiaojie Wang
Summary: Wheat is a staple food for about 40% of the world's population. However, global warming has posed challenges to wheat production, leading to water scarcity, soil salinization, and reduced plant fertility and yield. Genetic improvement of wheat for enhanced resistance to environmental stress is a promising option to address these challenges.
Article
Plant Sciences
Wanli You, Jinglin Zhang, Xueyin Ru, Feng Xu, Zhengguo Wu, Peng Jin, Yonghua Zheng, Shifeng Cao
Summary: This study investigated the effect of calcium chloride (CaCl2) treatment on GABA accumulation in fresh-cut cantaloupe and the underlying mechanisms. The results showed that CaCl2 treatment increased GABA content and the activities of GAD and SSADH enzymes, while reducing glutamate content and GABA-T activity. Additionally, CaCl2 treatment upregulated the expressions of CmCML11 and CmCAMTA5, as well as several GABA shunt genes, through the transcriptional activation by CmCAMTA5. Furthermore, the interaction between CmCML11 and CmCAMTA5 enhanced the transcriptional activation of GABA shunt genes. Overall, this study reveals that CaCl2 treatment promotes GABA accumulation in fresh-cut cantaloupe through the combined effect of CmCML11 and CmCAMTA5 in regulating the expressions of GABA shunt genes.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2024)
Article
Plant Sciences
Min Wang, Yupeng Wang, Xiaohui Wang, Guangwei Wei, Huiyi Yang, Xi Yang, Tinghai Shen, Huijie Qu, Sheng Fang, Ziming Wu
Summary: This study identified the high nitrogen use efficiency (NUE) black sesame variety 17-156 and analyzed its underlying physiological and molecular mechanisms. The results showed that 17-156 possesses a sophisticated nitrogen metabolizing machinery to uptake and assimilate higher quantities of inorganic nitrogen, simultaneously improving carbon metabolism and growth. Many important genes were up-regulated in 17-156 under high nitrogen condition. Additionally, 38 potential candidate genes were identified for future studies to improve sesame's NUE. These findings provide valuable resources for understanding the regulatory network of nitrogen metabolism and developing sesame cultivars with improved NUE.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2024)
Article
Plant Sciences
He Dong, Chongmei Xu, Chengtao Zhang, Li Zhang, Yaqin Yao, Suiqi Zhang
Summary: The study found that short cells in maize leaves not only improve leaf mechanical support and photosynthetic performance, enhance drought resistance, but also participate in stomatal regulation.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2024)
Article
Plant Sciences
Na Liu, Wenyan Shang, Mengxin Guan, Jibin Xiao, Guangxiang Tian, Baozhan Ma, Wenjing Shang, Xu Li, Shijia Zhao, Chuang Li, Kun Cheng, Wenming Zheng
Summary: This study cloned the full-length cDNA sequence of TaSPX3 gene in wheat and found that TaSPX3 responds to low phosphorus stress in multiple wheat genotypes. Overexpressing TaSPX3 can alleviate phosphorus deficiency symptoms and promote plant growth in Arabidopsis. The study also revealed the interaction of TaSPX3 with other genes related to the phosphorus starvation signaling pathway.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2024)
Article
Plant Sciences
Kongyuan Wu, Lizhen Wang, Zihan Wu, Ziqing Liu, Zengfei Li, Jun Shen, Shengjie Shi, Hong Liu, Christopher Rensing, Renwei Feng
Summary: Selenium (Se) can reduce uptake and translocation of cadmium (Cd) in plants by regulating root morphology. This study investigated the effects of Se(IV) on root exudates, root morphology, root endogenous hormones, and Cd uptake efficiency in rice under Cd stress. The results showed that Se(IV) significantly reduced Cd concentrations in shoots and roots, and decreased Cd uptake efficiency via root hairs. Se(IV) also affected root morphology, root exudates, and the synthesis of hormones like IAA and JA. However, transcriptome analysis revealed no upregulated differentially expressed genes (DEGs) in IAA synthesis.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2024)
Article
Plant Sciences
Di Wang, Huaifang Zhang, Xuefei Hu, Haizhen Zhang, Shuang Feng, Aimin Zhou
Summary: This study identified a cell number regulator gene called SlCNR8 in willow, which enhances resistance to trace metals in transgenic poplar seedlings. SlCNR8 reduces Cd uptake and accumulation, and can be used as a candidate gene for genetic improvement of phytostabilisation of trace metals.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2024)