Article
Plant Sciences
Lidan Zhang, Tingmi Yang, Ying Zhao, Zhitong Mo, Zhixiang Yuan, Yanchang Yang, Mingzhao Wang, Hongxia Liu
Summary: A bacterial pathogen strain was isolated from susceptible tissue of Hongyang variety kiwifruit in Zhongfeng Town, Ziyuan County, Guilin City, Guangxi, China. The pathogen was identified as Pectobacterium carotovorum subsp. actinidiae through morphological, physiological, biochemical, and genetic analysis. This is the first report of kiwifruit canker disease caused by P. carotovorum subsp. actinidiae in Guangxi, China. The study provides a preliminary understanding of the pathogen, which will contribute to future disease prevention and control.
Article
Agronomy
Hua Wang, Nana Wang, Yunxiao Tan, Qianqian Mi, Yiru Mao, Chao Zhao, Xiangrong Tian, Wei Liu, Lili Huang
Summary: In this study, a biocontrol strain P. polymyxa YLC1 that was isolated from the rhizosphere soil of asymptomatic kiwifruit showed excellent control effects on kiwifruit bacterial canker (KBC) both in laboratory and field tests. The active compound of strain YLC1 was identified as polymyxin B1, which inhibits various pathogenic bacteria. These findings suggest that P. polymyxa YLC1 has great potential for the development and application of biological control strategies.
PEST MANAGEMENT SCIENCE
(2023)
Article
Infectious Diseases
Yu-Rim Song, Nguyen Trung Vu, Jungkum Park, In Sun Hwang, Hyeon-Ju Jeong, Youn-Sup Cho, Chang-Sik Oh
Summary: The bacteriophage PPPL-1 shows promising antibacterial activity against Pseudomonas syringae pv. actinidiae (Psa) and could potentially be used as a biocontrol agent to effectively control bacterial canker disease in kiwifruit plants, including resistant isolates. PPPL-1 is stable in field soil and at low temperatures, making it a practical tool for agricultural applications. Further research is needed to explore the full potential of PPPL-1 in controlling Psa infections in kiwifruit orchards.
Article
Biochemistry & Molecular Biology
Wei Liu, Chao Zhao, Lu Liu, Dong Huang, Chao Ma, Rui Li, Lili Huang
Summary: A study found that there are 13 TGA genes in kiwifruit, and these genes play an important role in the resistance against kiwifruit bacterial canker, regulated by tissue and hormones.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Plant Sciences
Anna Fiorillo, Domenico Frezza, Gustavo Di Lallo, Sabina Visconti
Summary: The study found that the phage phi PSA2 can effectively control the occurrence and spread of kiwifruit canker, providing a possibility for the development of phage therapy based on this phage.
Article
Plant Sciences
Yangang Pei, Li Ma, Xiaojuan Zheng, Kaikai Yao, Xiangru Fu, Huabao Chen, Xiaoli Chang, Ming Zhang, Guoshu Gong
Summary: This study investigated the genetic characteristics of Pseudomonas syringae pv. actinidiae population from kiwifruit in Sichuan, China. The isolates were characterized using morphological features, multiplex-PCR, and multilocus sequence analysis (MLSA). The study revealed rich genetic diversity in P. syringae pv. actinidiae isolates from Sichuan.
Article
Microbiology
Yue Li, Qiaomei Zhu, Taihui Zhi, Rong Fan, Ting Xie, Zhibo Zhao, Youhua Long, Zhong Li
Summary: Bacterial canker disease poses a significant threat to kiwifruit cultivation, with a monomorphic subpopulation of Pseudomonas syringae pv. actinidiae biovar 3 responsible for a global pandemic. Through genetic analysis, it was found that certain isolates showed changes in pathogenicity and HR elicitation, likely due to transposon insertions in key regulatory genes related to the type III secretion system (T3SS) and effectors.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Zhuzhu Zhang, Youhua Long, Xianhui Yin, Sen Yang
Summary: This study revealed that sulfur application activated plant disease resistance through salicylic acid, effectively controlling kiwifruit canker and enhancing its immune response.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Analytical
Lingli Jiang, Longhui Luo, Mei Li, Ting Xie, Zhibo Zhao, Chao Kang, Dongmei Chen, Youhua Long
Summary: The aim of this study was to characterize the temporal and spatial changes of biopolymers in the petiole cell walls of healthy and cankered kiwifruit using confocal Raman microscopy (CRM) techniques. The results showed that the degradation rate of cellulose and lignin on the petiole cell wall reached about 25% after eight days of incubation. The study demonstrated the feasibility and application prospects of CRM combined with chemical classification algorithms and imaging analysis in the early warning and cellular-level characterization of kiwifruit canker.
MICROCHEMICAL JOURNAL
(2023)
Review
Virology
Jinyan Luo, Dejiang Dai, Luqiong Lv, Temoor Ahmed, Lei Chen, Yanli Wang, Qianli An, Guochang Sun, Bin Li
Summary: Kiwifruit production has been severely damaged by the bacterial plant pathogen Pseudomonas syringae pv. actinidiae (Psa) in recent decades, leading to significant economic losses worldwide. The use of antibiotics for control is becoming ineffective, making the development of phage-based biocontrol strategies crucial.
Article
Plant Sciences
Marta Nunes da Silva, Susana M. P. Carvalho, Ana M. Rodrigues, Aurelio Gomez-Cadenas, Carla Antonio, Marta W. Vasconcelos
Summary: Differential tolerance of Actinidia spp. to Pseudomonas syringae pv. actinidiae (Psa) may be linked to differential plant-defence strategies, phytohormones, and primary metabolism. Higher susceptibility of A. chinensis to Psa is attributed to inefficient activation of plant defences and metabolic impairments.
PLANT CELL AND ENVIRONMENT
(2022)
Article
Microbiology
Tao Liu, Xiaoli Ren, Guangyun Cao, Xia Zhou, Linhong Jin
Summary: Ethylicin effectively inhibits the growth of kiwifruit bacterial canker disease by limiting bacterial movement, destroying cell membrane, and inhibiting biofilm formation. It achieves this by up-regulating genes related to protein export and biofilm formation while down-regulating genes related to flagellar assembly.
Article
Microbiology
Nana Wang, Ning Han, Runze Tian, Jiliang Chen, Xiaoning Gao, Zhiran Wu, Yuqi Liu, Lili Huang
Summary: The type VI secretion system (T6SS) plays an important role in the pathogenicity of Pseudomonas syringae pv. actinidiae (Psa), with deletion mutants of T6SS core genes showing reduced pathogenicity. This reduction in pathogenicity is likely related to effects on bacterial competition, biofilm formation, and environmental adaptability, and a complex relationship exists between T6SS and T3SS.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Plant Sciences
Xiaojie Wang, Yawei Li, Yuanyuan Liu, Dongle Zhang, Min Ni, Bing Jia, Wei Heng, Zemin Fang, Li-wu Zhu, Pu Liu
Summary: The study showed that the plant hormone JA accumulated in the susceptible kiwifruit cultivar 'Hongyang' but decreased in the resistant cultivar 'Jinkui' in response to Psa. Transcriptomic and proteomic analyses of 'Jinkui' identified multiple genes and proteins involved in the response to Psa infection, including the R2R3-MYB transcription factor AcMYB16, which acts as a repressor in regulating kiwifruit's response to bacterial pathogens.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Multidisciplinary Sciences
Siyi Deng, Wei Chang, Yawei Que, Jun Liu, Hua Wang
Summary: This study investigated the movement path and survival time of Pseudomonas syringae pv. actinidiae (Psa) in kiwifruit leaves and branches. Results showed that Psa spread longitudinally in the branches and leaves and its survival in detached kiwifruit leaves was significantly affected by environmental conditions. Psa could survive the longest, up to 43 days, in detached kiwifruit leaves with high humidity (above 80%) at 5 degrees C.
Article
Plant Sciences
Nay C. Dia, Lucas Moriniere, Bart Cottyn, Eduardo Bernal, Jonathan M. Jacobs, Ralf Koebnik, Ebrahim Osdaghi, Neha Potnis, Joel F. Pothier
Summary: The passage provides information about the taxonomy, host range, epidemiology, and control measures of Xanthomonas hortorum.
MOLECULAR PLANT PATHOLOGY
(2022)
Article
Plant Sciences
Joel F. Pothier, Monika Kaluzna, Andjelka Prokic, Aleksa Obradovic, Fabio Rezzonico
Summary: Xanthomonas arboricola pv. corylina is the causal agent of hazelnut bacterial blight and has been classified as an A2 quarantine pathogen in Europe. In this study, three isolates from different geographic regions and time points were sequenced to obtain complete genome and plasmid sequences, aiming to improve the understanding of this pathogen's biology.
Article
Plant Sciences
Sadegh Zarei, S. Mohsen Taghavi, Touraj Rahimi, Hamzeh Mafakheri, Neha Potnis, Ralf Koebnik, Marion Fischer-Le Saux, Joel F. Pothier, Ana Palacio Bielsa, Jaime Cubero, Perrine Portier, Marie-Agnes Jacques, Ebrahim Osdaghi
Summary: A recent study evaluated the taxonomic status of Xanthomonas arboricola, a group of fruit tree pathogens. The study used DNA similarity indexes and core genome-based phylogeny to determine the members of the species. The results revealed that two pathovars should be elevated to the rank of a new species. Additionally, other mislabeled strains belong to previously described species or represent novel species awaiting formal description.
Article
Plant Sciences
Monika Kaluzna, Joel F. Pothier
Article
Multidisciplinary Sciences
Pierre H. H. Schneeberger, Morgan Gueuning, Sophie Welsche, Eveline Huerlimann, Julian Dommann, Cecile Haeberli, Juerg E. Frey, Somphou Sayasone, Jennifer Keiser
Summary: This study analyzed the microbiome of Trichuris trichiura and hookworm-infected patients and found an association between different enterotypes and treatment efficacy. The pre-treatment enterotype was found to predict the treatment outcome of combination therapy for both T. trichiura and hookworm infections.
NATURE COMMUNICATIONS
(2022)
Article
Microbiology
Michela Ruinelli, Jochen Blom, Theo H. M. Smits, Joeel F. Pothier
Summary: A comparative genomics approach identified genes significantly associated with strains isolated from Prunus spp., with the HopAY gene predicted to encode a C58 cysteine protease being highly related to these strains, supporting co-evolution and host adaptation patterns.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Ashley M. Rooney, Amogelang R. Raphenya, Roberto G. Melano, Christine Seah, Noelle R. Yee, Derek R. MacFadden, Andrew G. McArthur, Pierre H. H. Schneeberger, Bryan Coburn
Summary: This study systematically characterized the performance of short-read Illumina sequencing for antimicrobial resistance gene (ARG) detection in metagenomic samples, providing important information for the study design and assessment of human, animal, and environmental metagenomic antimicrobial resistance studies.
Article
Microbiology
Kayla Gisela Silva, Leonor Martins, Miguel Teixeira, Joel F. Pothier, Fernando Tavares
Summary: This study developed a DNA-marker-based method for the detection and genotyping of X. euroxanthea and proposed a workflow for the selection of species-specific detection markers. The method can track the presence of X. euroxanthea in microbial consortia and contribute to the control of phytopathogenic strains.
Article
Microbiology
Leandra E. Knecht, Yannick Born, Cosima Pelludat, Joel F. Pothier, Theo H. M. Smits, Martin J. Loessner, Lars Fieseler
Summary: The widespread use of antibiotics has led to an increase in antibiotic resistant bacteria. As a result, researchers are exploring effective alternatives, such as bacteriophages. However, phage resistance can still occur. This study found that different phages had varying effects on the resistance of Erwinia amylovora CFBP 1430, the bacteria responsible for fire blight. Additionally, a mutant strain, 1430(Y2R), exhibited cross-resistance to certain phages and altered LPS structures.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Eliska Hakalova, Jana Cechova, Dorota A. Tekielska, Ales Eichmeier, Joel F. Pothier
Summary: The seed-borne bacterium Xanthomonas campestris pv. campestris (Xcc) is a serious problem in agricultural production of cruciferous plants. Biocontrol methods, such as the use of carvacrol and thymol, show promise in managing black rot disease caused by Xcc.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Arburon Sulja, Joel F. Pothier, Jochen Blom, Chiaraluce Moretti, Roberto Buonaurio, Fabio Rezzonico, Theo H. M. Smits
Summary: In this study, the whole genome of Pantoea agglomerans DAPP-PG 734, which was isolated from knots in olive trees caused by Pseudomonas savastanoi pv. savastanoi DAPP-PG 722, was sequenced and analyzed. Gene clusters related to enhanced interaction and antibiotic synthesis were identified. The in-depth analysis of the genome suggested that Pantoea agglomerans DAPP-PG 734 is an endophytic bacterium with biocontrol activity.
Article
Environmental Sciences
Zala Schmautz, Jean-Claude Walser, Carlos A. Espinal, Florentina Gartmann, Ben Scott, Joel F. Pothier, Emmanuel Frossard, Ranka Junge, Theo H. M. Smits
Summary: Aquaponics systems have unique microbial communities in different compartments, with nitrifying bacteria identified in the hydroponic compartments. The composition of microbial communities varied over time in the anaerobic compartment, with more archaeal reads obtained from sludge samples. Fresh fish feces had lower bacterial diversity with a discrete gut flora composition. The most pronounced differences in microbial community compositions were observed between the aerobic and anaerobic loops of the system.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Editorial Material
Microbiology
Joana Costa, Joel F. Pothier, Jens Boch, Emilio Stefani, Ralf Koebnik
Article
Oncology
A. Spreaflco, A. A. Heirali, D. V. Araujo, T. J. Tan, M. Oliva, P. H. H. Schneeberger, B. Chen, M. K. Wong, L. -A. Stayner, A. R. Hansen, S. D. Saibil, B. X. Wang, K. Cochrane, K. Sherriff, E. Allen-Vercoe, W. Xu, L. L. Siu, B. Coburn
Summary: This study evaluated the safety, tolerability, and ecological responses of an orally administered 30-species microbial consortium (MET4) as an alternative to immune checkpoint inhibitor (ICI) treatment. The trial achieved its primary safety and tolerability outcomes, and observed variations in MET4 species relative abundance after administration. The results justify further development of microbial consortia as a therapeutic co-intervention for ICI treatment in cancer.
ANNALS OF ONCOLOGY
(2023)
Article
Plant Sciences
Tony Reglinski, Joel L. L. Vanneste, Magan M. M. Schipper, Deirdre A. A. Cornish, Janet Yu, Jenny M. M. Oldham, Christina Fehlmann, Frank Parry, Duncan Hedderley
Summary: The effect of pre-flowering application of ASM on defence gene expression in kiwifruit plants is known, however, the effect of postharvest ASM on defence gene expression is unknown. In this study, the expression of eight defence marker genes was measured in kiwifruit vine leaves after postharvest treatment with ASM and/or copper.
