Generation of chromosomal deletions in dicotyledonous plants employing a user-friendly genome editing toolkit

Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9

(2016) John G Doench et al.   NATURE BIOTECHNOLOGY

An Effective Strategy for Reliably Isolating Heritable and Cas9 -Free Arabidopsis Mutants Generated by CRISPR/Cas9-Mediated Genome Editing

(2016) Xiuhua Gao et al.   PLANT PHYSIOLOGY

Arabidopsis thaliana DM2h (R8) within the Landsberg RPP1-like Resistance Locus Underlies Three Different Cases of EDS1-Conditioned Autoimmunity

(2016) Johannes Stuttmann et al.   PLoS Genetics

The CRISPR/Cas9 system for plant genome editing and beyond

(2015) Luisa Bortesi et al.   BIOTECHNOLOGY ADVANCES

Editing plant genomes with CRISPR/Cas9

(2015) Khaoula Belhaj et al.   CURRENT OPINION IN BIOTECHNOLOGY

A Robust CRISPR/Cas9 System for Convenient, High-Efficiency Multiplex Genome Editing in Monocot and Dicot Plants

(2015) Xingliang Ma et al.   Molecular Plant

Seed filling in domesticated maize and rice depends on SWEET-mediated hexose transport

(2015) Davide Sosso et al.   NATURE GENETICS

Improving cold storage and processing traits in potato through targeted gene knockout

(2015) Benjamin M. Clasen et al.   PLANT BIOTECHNOLOGY JOURNAL

Development of germ-line-specific CRISPR-Cas9 systems to improve the production of heritable gene modifications inArabidopsis

(2015) Yanfei Mao et al.   PLANT BIOTECHNOLOGY JOURNAL

A multiplex CRISPR/Cas9 platform for fast and efficient editing of multiple genes in Arabidopsis

(2015) Zhengjing Zhang et al.   PLANT CELL REPORTS

A CRISPR/Cas9 Toolbox for Multiplexed Plant Genome Editing and Transcriptional Regulation

(2015) Levi G. Lowder et al.   PLANT PHYSIOLOGY

Auxin binding protein 1 (ABP1) is not required for either auxin signaling orArabidopsisdevelopment

(2015) Yangbin Gao et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Boosting CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing system

(2015) Kabin Xie et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Egg cell-specific promoter-controlled CRISPR/Cas9 efficiently generates homozygous mutants for multiple target genes in Arabidopsis in a single generation

(2015) Zhi-Ping Wang et al.   GENOME BIOLOGY

CasOT: a genome-wide Cas9/gRNA off-target searching tool

(2014) A. Xiao et al.   BIOINFORMATICS

Highly efficient targeted chromosome deletions using CRISPR/Cas9

(2014) Zuyong He et al.   BIOTECHNOLOGY AND BIOENGINEERING

Species-wide Genetic Incompatibility Analysis Identifies Immune Genes as Hot Spots of Deleterious Epistasis

(2014) Eunyoung Chae et al.   CELL

Successful Transient Expression of Cas9 and Single Guide RNA Genes in Chlamydomonas reinhardtii

(2014) Wenzhi Jiang et al.   EUKARYOTIC CELL

Characterization of Genomic Deletion Efficiency Mediated by Clustered Regularly Interspaced Palindromic Repeats (CRISPR)/Cas9 Nuclease System in Mammalian Cells

(2014) Matthew C. Canver et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Multiplexed and Programmable Regulation of Gene Networks with an Integrated RNA and CRISPR/Cas Toolkit in Human Cells

(2014) Lior Nissim et al.   MOLECULAR CELL

CRISPR-P: A Web Tool for Synthetic Single-Guide RNA Design of CRISPR-System in Plants

(2014) Yang Lei et al.   Molecular Plant

Rational design of highly active sgRNAs for CRISPR-Cas9–mediated gene inactivation

(2014) John G Doench et al.   NATURE BIOTECHNOLOGY

The CRISPR/Cas system can be used as nuclease forin plantagene targeting and as paired nickases for directed mutagenesis in Arabidopsis resulting in heritable progeny

(2014) Simon Schiml et al.   PLANT JOURNAL

Both CRISPR/Cas-based nucleases and nickases can be used efficiently for genome engineering inArabidopsis thaliana

(2014) Friedrich Fauser et al.   PLANT JOURNAL

Efficient Gene Editing in Tomato in the First Generation Using the Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-Associated9 System

(2014) C. Brooks et al.   PLANT PHYSIOLOGY

Site-directed mutagenesis in Arabidopsis thaliana using dividing tissue-targeted RGEN of the CRISPR/Cas system to generate heritable null alleles

(2014) Youbong Hyun et al.   PLANTA

Efficient CRISPR/Cas9-Mediated Gene Editing in Arabidopsis thaliana and Inheritance of Modified Genes in the T2 and T3 Generations

(2014) WenZhi Jiang et al.   PLoS One

The new frontier of genome engineering with CRISPR-Cas9

(2014) J. A. Doudna et al.   SCIENCE

A TAL effector repeat architecture for frameshift binding

(2014) Annekatrin Richter et al.   Nature Communications

A Golden Gate Modular Cloning Toolbox for Plants

(2014) Carola Engler et al.   ACS Synthetic Biology

Structural Basis for Signaling by Exclusive EDS1 Heteromeric Complexes with SAG101 or PAD4 in Plant Innate Immunity

(2013) Stephan Wagner et al.   Cell Host & Microbe

Efficient genome editing in plants using a CRISPR/Cas system

(2013) Zhengyan Feng et al.   CELL RESEARCH

Self-processing of ribozyme-flanked RNAs into guide RNAsin vitroandin vivofor CRISPR-mediated genome editing

(2013) Yangbin Gao et al.   Journal of Integrative Plant Biology

DNA targeting specificity of RNA-guided Cas9 nucleases

(2013) Patrick D Hsu et al.   NATURE BIOTECHNOLOGY

High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells

(2013) Yanfang Fu et al.   NATURE BIOTECHNOLOGY

Chromosomal deletions and inversions mediated by TALENs and CRISPR/Cas in zebrafish

(2013) An Xiao et al.   NUCLEIC ACIDS RESEARCH

Plant genome editing made easy: targeted mutagenesis in model and crop plants using the CRISPR/Cas system

(2013) Khaoula Belhaj et al.   Plant Methods

Mechanism of Eukaryotic RNA Polymerase III Transcription Termination

(2013) S. Nielsen et al.   SCIENCE

FairyTALE: A High-Throughput TAL Effector Synthesis Platform

(2013) Jing Liang et al.   ACS Synthetic Biology

Targeted Mutagenesis of Arabidopsis thaliana Using Engineered TAL Effector Nucleases

(2013) Michelle Christian et al.   G3-Genes Genomes Genetics

RNA-guided genetic silencing systems in bacteria and archaea

(2012) Blake Wiedenheft et al.   NATURE

Analysis of new type III effectors fromXanthomonasuncovers XopB and XopS as suppressors of plant immunity

(2012) Sebastian Schulze et al.   NEW PHYTOLOGIST

Activation ofR-mediated innate immunity and disease susceptibility is affected by mutations in a cytosolicO-acetylserine (thiol) lyase in Arabidopsis

(2012) Jibran Tahir et al.   PLANT JOURNAL

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

A Modular Cloning System for Standardized Assembly of Multigene Constructs

(2011) Ernst Weber et al.   PLoS One

Assembly of Designer TAL Effectors by Golden Gate Cloning

(2011) Ernst Weber et al.   PLoS One

Transcriptional Activators of Human Genes with Programmable DNA-Specificity

(2011) René Geiβler et al.   PLoS One

SAG101 Forms a Ternary Complex with EDS1 and PAD4 and Is Required for Resistance Signaling against Turnip Crinkle Virus

(2011) Shifeng Zhu et al.   PLoS Pathogens

XanthomonasAvrBs3 Family-Type III Effectors: Discovery and Function

(2010) Jens Boch et al.   Annual Review of Phytopathology

A mutation in the cytosolic O-acetylserine (thiol) lyase induces a genome-dependent early leaf death phenotype in Arabidopsis

(2010) Reza Shirzadian-Khorramabad et al.   BMC PLANT BIOLOGY

Targeting DNA Double-Strand Breaks with TAL Effector Nucleases

(2010) M. Christian et al.   GENETICS

Natural variation at Strubbelig Receptor Kinase 3 drives immune-triggered incompatibilities between Arabidopsis thaliana accessions

(2010) Rubén Alcázar et al.   NATURE GENETICS

High-frequency modification of plant genes using engineered zinc-finger nucleases

(2009) Jeffrey A. Townsend et al.   NATURE

A rapid and non-destructive screenable marker, FAST, for identifying transformed seeds ofArabidopsis thaliana

(2009) Takashi L. Shimada et al.   PLANT JOURNAL

Breaking the Code of DNA Binding Specificity of TAL-Type III Effectors

(2009) J. Boch et al.   SCIENCE

Incremental steps toward incompatibility revealed by Arabidopsis epistatic interactions modulating salicylic acid pathway activation

(2008) R. Alcazar et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA