Article
Plant Sciences
Amit K. Singh, Maria Santos-Merino, Jonathan K. Sakkos, Berkley J. Walker, Daniel C. Ducat
Summary: This study investigates the systemic changes in a model cyanobacterium caused by alterations in carbon availability. The results suggest that Rubisco activity and organization are key variables connected to regulatory pathways involved in metabolic balancing in cyanobacteria.
Article
Biotechnology & Applied Microbiology
Yu Dan, Jiahui Sun, Shanshan Zhang, Yannan Wu, Shaoming Mao, Guodong Luan, Xuefeng Lu
Summary: This study successfully regulated glycogen content in cyanobacteria by enhancing glycogen degradation, leading to increased sucrose production. These findings provide new insights into cyanobacterial glycogen metabolism engineering and will inspire the development of novel metabolic engineering approaches for efficient photosynthetic biosynthesis.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biochemical Research Methods
Mingyi Zhang, Quan Luo, Huili Sun, Jacques Fritze, Guodong Luan, Xuefeng Lu
Summary: This study developed an inducible cyanobacteria cell factory using the ssrADAS-SspB-ClpXPEc system to achieve rapid degradation of target proteins. Additionally, an efficient and stringent inducible gene expression system was obtained, laying the foundation for future dynamic metabolic engineering efforts.
ACS SYNTHETIC BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Anna Paola Casazza, Alessandro Lombardi, Barbara Menin, Stefano Santabarbara
Summary: The exogenous crtZ gene was expressed in Synechococcus elongatus PCC 7942 to engineer the carotenoid metabolic pathway and increase zeaxanthin content. However, this led to a growth impairment and cellular stress.
PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Bo Wang, Cristal Zuniga, Michael T. Guarnieri, Karsten Zengler, Michael Betenbaugh, Jamey D. Young
Summary: This study investigates the global metabolic flux alterations in a sucrose-secreting strain of cyanobacteria and identifies a metabolic bottleneck that limits carbon fixation and sucrose biosynthesis. The study demonstrates the usefulness of combining targeted metabolomics, 13C metabolic flux analysis, and genome-scale modeling to improve flux predictions. Additionally, the study shows that heterologous overexpression can mitigate the metabolic bottleneck.
METABOLIC ENGINEERING
(2023)
Article
Microbiology
Maria Santos-Merino, Alvaro Gargantilla-Becerra, Fernando de la Cruz, Juan Nogales
Summary: In this study, a more comprehensive and functional model of a freshwater cyanobacterium was constructed, known as iMS837. The model provides detailed insights into the metabolism of the bacterium, including fatty acid biosynthesis, oxidative phosphorylation, photosynthesis, and transport, among others. The findings suggest that this model can be used to identify suitable metabolic engineering strategies to improve the production of non-native omega-3 fatty acids.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Microbiology
Sadaf Riaz, Ying Jiang, Meng Xiao, Dawei You, Anna Klepacz-Smolka, Faiz Rasul, Maurycy Daroch
Summary: This study optimized the cultivation and natural transformation protocol for the important cyanobacterium Synechococcus elongatus PCC 7942. By adjusting agar concentration and overexpressing catalase gene, the growth of Synechococcus 7942 on solid media was improved. High temperature and phosphate depletion conditions resulted in fewer genome copies and potential to produce fully segregated mutants. Higher antibiotic concentrations improved the selection of homozygous transformants.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Rees Rillema, Y. Hoang, Joshua S. MacCready, Anthony G. Vecchiarelli
Summary: Research shows that improper distribution of carboxysomes in cyanobacteria can result in slower cell growth, cell elongation, asymmetric cell division, and elevated levels of cellular Rubisco. Additionally, even wild-type S. elongatus undergoes cell elongation and asymmetric cell division when grown at lower temperatures or switched from high to ambient CO2 conditions. This suggests that the McdAB system plays a crucial role in maintaining the carbon fixation efficiency of Rubisco in cyanobacteria.
Article
Biology
Maria Santos-Merino, Raquel Gutierrez-Lanza, Juan Nogales, Jose Luis Garcia, Fernando de la Cruz
Summary: This study analyzed enzymes in cyanobacteria related to fatty acid synthesis and found that increasing the expression of these enzymes can enhance the production of C18 fatty acids. However, coexpression with other desaturases did not improve the production of alpha-linolenic acid, and attempts to clone another enzyme for stearidonic acid synthesis were also unsuccessful.
Article
Microbiology
Ryan Simkovsky, Rami Parnasa, Jingtong Wang, Elad Nagar, Eli Zecharia, Shiran Suban, Yevgeni Yegorov, Boris Veltman, Eleonora Sendersky, Rakefet Schwarz, Susan S. Golden
Summary: In this study, transcriptomics and phenomics screens were used to identify genes involved in biofilm formation and regulation in Synechococcus elongatus PCC 7942. The study also revealed the transcriptomic adaptation to the biofilm state and compared the effectiveness of these two approaches for gene discovery.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Plant Sciences
Pankaj K. Maurya, Soumila Mondal, Vinod Kumar, Shailendra P. Singh
Summary: Cyanobacteria, specifically Synechococcus elongatus PCC 7942, show varying responses to different light wavelengths, with green and blue light negatively affecting their fitness and growth but increasing lipid content and changing cell morphology.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2023)
Article
Biology
ShylajaNaciyar Mohandass, Mangalalakshmi Ragavan, Dineshbabu Gnanasekaran, Uma Lakshmanan, Prabaharan Dharmar, Sushanta Kumar Saha
Summary: Synthetic azo dyes are widely used but their improper discharge poses a threat to the environment. Genetically modified cyanobacteria with enhanced H2O2 accumulation through SOD overexpression can effectively degrade these dyes, suggesting a valuable bioremediation strategy.
Article
Biotechnology & Applied Microbiology
Shu-Yan Wang, Xin Li, Shu-Guang Wang, Peng-Fei Xia
Summary: Cyanobacteria have the potential to convert atmospheric carbon dioxide into biofuels, chemicals, and food. However, the development of genome-editing tools for cyanobacteria has been slower compared to other microbial chassis. In this study, we successfully adapted base editing using the CRISPR-Cas system for cyanobacteria, enabling precise and efficient genome editing. We demonstrated multiplex base editing in a model cyanobacterium and manipulated the glycogen metabolic pathway to enhance the production of chemicals and food from carbon dioxide. This work represents the first report of base editing in cyanobacteria and provides a paradigm for using CRISPR-Cas systems in bacteria, with the potential to accelerate metabolic engineering and synthetic biology to address global climate change.
METABOLIC ENGINEERING
(2023)
Article
Immunology
Wen Peng, Qunjing Bao, Rui Jia, Peimin He
Summary: This study constructed transgenic cyanobacteria expressing recombinant protein VP28-mOrange using the receptor system of Synechococcus elongatus PCC 7942 and explored its metabolic cycle and protective effect against WSSV in Litopenaeus vannamei. The results showed that the recombinant protein had a weak fluorescence signal in the intestine after the shrimp stopped consuming transgenic cyanobacteria and its protective effect decreased with the time of WSSV challenge.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Plant Sciences
Takayuki Sakamoto, Nobuyuki Takatani, Kintake Sonoike, Haruhiko Jimbo, Yoshitaka Nishiyama, Tatsuo Omata
Summary: In this study, it was found that the loss of P-II in cyanobacteria increased the sensitivity of PSII to ammonium and stimulated the formation of reactive oxygen species in mutant cells, leading to growth defects. The aberrant action of uncontrolled PipX impaired the electron transport reactions in both the reducing and oxidizing sides of Q(A), as shown by the unusual chlorophyll fluorescence profile observed in the targeted P-II mutant.
PLANT AND CELL PHYSIOLOGY
(2021)
Article
Biochemical Research Methods
Yifan Yang, Lei Xu, Zheyun Feng, Jeffrey A. Cruz, Linda J. Savage, David M. Kramer, Jin Chen
Article
Biochemistry & Molecular Biology
Deserah D. Strand, Nicholas Fisher, David M. Kramer
JOURNAL OF BIOLOGICAL CHEMISTRY
(2017)
Article
Plant Sciences
Christopher G. Oakley, Linda Savage, Samuel Lotz, G. Rudd Larson, Michael F. Thomashow, David M. Kramer, Douglas W. Schemske
JOURNAL OF EXPERIMENTAL BOTANY
(2018)
Article
Plant Sciences
Siddhartha Dutta, Jeffrey A. Cruz, Saif M. Imran, Jin Chen, David M. Kramer, Katherine W. Osteryoung
JOURNAL OF EXPERIMENTAL BOTANY
(2017)
Article
Biology
Kang-Ling Liao, Roger D. Jones, Patrick McCarter, Meral Tunc-Ozdemir, James A. Draper, Timothy C. Elston, David Kramer, Alan M. Jones
JOURNAL OF THEORETICAL BIOLOGY
(2017)
Article
Biology
Geoffry A. Davis, A. William Rutherford, David M. Kramer
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
(2017)
Article
Plant Sciences
Stefanie Tietz, Christopher C. Hall, Jeffrey A. Cruz, David M. Kramer
PLANT CELL AND ENVIRONMENT
(2017)
Article
Plant Sciences
Ben Lucker, Eliezer Schwarz, Sebastian Kuhlgert, Elisabeth Ostendorf, David M. Kramer
Article
Plant Sciences
Alejandro Morales, Xinyou Yin, Jeremy Harbinson, Steven M. Driever, Jaap Molenaar, David M. Kramer, Paul C. Struik
Review
Biotechnology & Applied Microbiology
Clifford J. Unkefer, Richard T. Sayre, Jon K. Magnuson, Daniel B. Anderson, Ivan Baxter, Ian K. Blaby, Judith K. Brown, Michael Carleton, Rose Ann Cattolico, Taraka Dale, Timothy P. Devarenne, C. Meghan Downes, Susan K. Dutcher, David T. Fox, Ursula Goodenough, Jan Jaworski, Jonathan E. Holladay, David M. Kramer, Andrew T. Koppisch, Mary S. Lipton, Babetta L. Marrone, Margaret McCormick, Istvan Molnar, John B. Mott, Kimberly L. Ogden, Ellen A. Panisko, Matteo Pellegrini, Juergen Polle, James W. Richardson, Martin Sabarsky, Shawn R. Starkenburg, Gary D. Stormo, Munehiro Teshima, Scott N. Twary, Pat J. Unkefer, Joshua S. Yuan, Jose A. Olivares
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2017)
Article
Plant Sciences
Deserah D. Strand, Aaron K. Livingston, Mio Satoh-Cruz, Tyson Koepke, Heather M. Enlow, Nicholas Fisher, John E. Froehlich, Jeffrey A. Cruz, Deepika Minhas, Kim K. Hixson, Kaori Kohzuma, Mary Lipton, Amit Dhingra, David M. Kramer
FRONTIERS IN PLANT SCIENCE
(2017)
Article
Computer Science, Artificial Intelligence
Xi Yin, Xiaoming Liu, Jin Chen, David M. Kramer
IEEE TRANSACTIONS ON PATTERN ANALYSIS AND MACHINE INTELLIGENCE
(2018)
Article
Biochemistry & Molecular Biology
Zhi-Yan Du, Ben F. Lucker, Krzysztof Zienkiewicz, Tarryn E. Miller, Agnieszka Zienkiewicz, Barbara B. Sears, David M. Kramer, Christoph Benning
Article
Plant Sciences
Rikard Fristedt, Chen Hu, Nicole Wheatley, Laura M. Roy, Rebekka M. Wachter, Linda Savage, Jeremy Harbinson, David M. Kramer, Sabeeha S. Merchant, Todd Yeates, Roberta Croce
Article
Plant Sciences
Jiying Li, Stefanie Tietz, Jeffrey A. Cruz, Deserah D. Strand, Ye Xu, Jin Chen, David M. Kramer, Jianping Hu