Article
Biochemistry & Molecular Biology
Marvin Droste, Tobias Tertel, Stefanie Jeruschke, Robin Dittrich, Evangelia Kontopoulou, Bernd Walkenfort, Verena Boerger, Peter F. Hoyer, Anja K. Buescher, Basant K. Thakur, Bernd Giebel
Summary: Small extracellular vesicles isolated from urine (uEVs) are considered potential biomarkers, with different preparation strategies available. Analysis using nanoparticle tracking analysis (NTA), Western blot, and imaging flow cytometry (IFCM) showed that urine mainly contains CD9(+) sEV population, storage temperature affects CD9(+) sEV recovery, and challenges the reliability of traditional NTA for evaluating EV preparation methods.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Multidisciplinary
Siwoo Cho, Johan Yi, Yongmin Kwon, Hyejin Kang, Chungmin Han, Jaesung Park
Summary: The study demonstrates a fluorescence-based nanoparticle tracking analysis (NTA) system for characterizing the size and membrane protein expression of individual extracellular vesicles (EVs). By comparing scattering and fluorescent images, membrane proteins labeled on EVs can be detected.
Article
Chemistry, Medicinal
Shiho Yahata, Mio Hirose, Takayo Ueno, Hiroki Nagumo, Kumiko Sakai-Kato
Summary: Sample concentration is critical for obtaining reproducible data of small extracellular vesicles (sEVs) as shown in the study. Analysis of the linearity range revealed that the linearity range for sEVs is narrower compared to liposomes.
CHEMICAL & PHARMACEUTICAL BULLETIN
(2021)
Article
Nanoscience & Nanotechnology
Yann Pellequer, Gilbert Zanetta, Jean-Michel Rebibou, Isabelle Severin, Marie-Christine Chagnon, Gernot Zissel, Fabrice Neiers, Renaud Seigneuric
Summary: The current frontiers in Biology, Medicine, and Pharmacy are focused on the nanoscale, particularly in extracting, synthesizing, visualizing, and characterizing nanoparticles. Various instruments are being developed to quantify and compare nanoparticles sizes. This study introduces a statistical approach for comparing different groups of biological samples with potential applications in different fields such as determining the size differences in liposomes and extracellular vesicles.
APPLIED NANOSCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Clement Auger, Aude Brunel, Tiffany Darbas, Hussein Akil, Aurelie Perraud, Gaelle Begaud, Barbara Bessette, Niki Christou, Mireille Verdier
Summary: Cancer cells secrete extracellular vesicles to communicate with their environment, with RAB27A playing a key role in this process. The study found that NanoSight technology was insufficient in detecting a decrease in EVs secretion, leading to exploration of alternative methods. Tracking EVs secretion using NTA revealed limitations in assessing secretion decrease.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Multidisciplinary
Bo Jiang, Hui Yang, Ying Guo, Cong Liu, Hua Song, Panpan Zhou, Haiwei Zhang, Kangxin Zhou, Yong Guo, Hongwei Chen
Summary: In this study, positively-charged carbon quantum dots (CA-PEI CQDs) were synthesized and tested for their biocompatibility with human MSCs and blood cells. These CA-PEI CQDs not only effectively image MSCs in vitro, located in the cytoplasms of MSCs, but can also track the migration of MSCs in vivo in lupus MRL/lpr mice. This research provides valuable insights into the molecular mechanisms behind MSC therapy for alleviating diseases.
NEW JOURNAL OF CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Kornelia Salaga-Zaleska, Agnieszka Kuchta, Beata Bzoma, Gabriela Chyla-Danil, Anna Safianowska, Agata Ploska, Leszek Kalinowski, Alicja Debska-Slizien, Maciej Jankowski
Summary: Urinary extracellular vesicles (uEVs) can serve as specific markers of kidney damage in different pathological conditions. Nanoparticle-tracking analysis (NTA) is considered the most useful method for uEV analysis as it can measure particles below 300 nm. However, the NTA method's application for uEV analysis is poorly described and requires meticulous sample preparation, experimental adjustment, and an understanding of its limitations. This study demonstrates the usefulness of NTA for uEV determination and provides solutions to the encountered problems during analysis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Pharmacology & Pharmacy
Mohammad Amin Rajizadeh, Sina Motamedy, Yousof Mir, Faezeh Akhgarandouz, Mohammad Hadi Nematollahi, Akram Nezhadi
Summary: This review discusses the therapeutic and diagnostic potential of vesicular nanoparticles in brain diseases. The different categorizations of colloidal drug delivery systems are explained, and the characteristics of vesicular drug delivery carriers are described. The recent advances in therapeutic vesicle-based carriers, such as liposomes, niosomes, exosomes, and virosomes, for the treatment of central nervous system disorders are summarized. Additionally, various routes of drug administration, including oral consumption, intraventricular, intraperitoneal, intravenous, etc., for targeting the brain are discussed.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Paulina Borzdzilowska, Ilona Bednarek
Summary: This study evaluated the effects of cisplatin and a plant extract alpha-mangostin on ovarian cancer cells and the cancer cell microenvironment. The results showed that the combination of alpha-mangostin and cisplatin reduced the concentration of cisplatin while maintaining the same cytotoxic effect. Additionally, the composition of exosomes released from drug-treated cancer cells may be different from untreated cells.
Article
Biophysics
Vincenzo De Leo, Anna Maria Maurelli, Livia Giotta, Lucia Catucci
Summary: The rapid development of nanotechnology has enabled the production of a variety of nanomaterials with outstanding properties, and the combination with liposomes has shown great potential in the fields of biology and biomedicine.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2022)
Review
Chemistry, Analytical
Yusong Wu, Yuqing Wang, Yanjun Lu, Xiaomei Luo, Yinghong Huang, Ting Xie, Christian Pilarsky, Yuanye Dang, Jianye Zhang
Summary: This review provides an overview of the conventional methods and microfluidic-based technologies for exosome separation, with a focus on the efficiency of exosome isolation by microfluidic devices. Additionally, advances in integrated microfluidics technologies for exosome separation and analysis are introduced.
Article
Biochemistry & Molecular Biology
Chanel A. Mosby, Natalia Perez Devia, Melissa K. Jones
Summary: There are various methods for quantifying bacterial outer membrane vesicles, but comparing results can be challenging. Common methods for vesicle quantification include lipid quantification, protein quantification, and direct vesicle enumeration. Different buffers and lysis buffers are also used. The microBCA assay has the least variability and strongest correlation with nanoparticle tracking analysis (NTA) for protein concentration and particle number.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Applied
Laura Hanley, Saeed M. Ghazani, Alejandro G. Marangoni
Summary: The spontaneous formation of phospholipid vesicles was explored for the production of liposomal encapsulations of bioactive compounds. Stable vesicles were produced from unrefined soybean and sunflower lecithin, and their structure and encapsulation capacity were evaluated. These vesicles demonstrated high thermal and oxidative stability, making them suitable for food applications.
Review
Nanoscience & Nanotechnology
Yuying Zhao, Hanxu Tan, Juping Zhang, Bo Pan, Neng Wang, Tongkai Chen, Yafei Shi, Zhiyu Wang
Summary: Lipid-structured vesicles have been used in drug delivery for over 50 years. There are two main categories: synthetic lipid vesicles (SLNVEs) and vesicles of mammalian origin (MDVEs). SLNVEs can transport anti-cancer drugs but have poor biocompatibility. MDVEs have excellent biocompatibility but lack stability. Plant-derived vesicles (PDVEs) combine the advantages of SLNVEs and MDVEs and are considered an ideal candidate for drug preparation and transport.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2023)
Article
Materials Science, Multidisciplinary
Pratibha Sharma, Rajinder K. Dhamija, Tapas Chandra Nag, Ambuj Roy, Krishna Kishore Inampudi
Summary: Small extracellular vesicles (sEVs) or exosomes have potential applications in translational research for clinical diagnostics, biomarker discovery, drug delivery, and therapeutic strategies. Variations in morphology and distribution are important for their biological function integrity, influenced by factors such as cell stress, inflammation, and secretion. In this study, we observed morphological variations in sEVs isolated from patients with atherosclerotic cardiovascular disease (AsCVD) in blood plasma, saliva, and urine samples compared to healthy controls. These variations provide insights into the role of sEVs in disease processes.
MICROSCOPY AND MICROANALYSIS
(2023)
Article
Biology
Anna Leabourn Boss, Tanvi Damani, Tayla J. Wickman, Larry W. Chamley, Joanna L. James, Anna E. S. Brooks
Summary: Researchers used flow cytometry to phenotypically characterize mesenchymal cells in the placenta and identified four distinct subsets. They also discovered that some mesenchymal cells undergo phenotypic convergence in ex-vivo culture. This study highlights the importance of detailed cell phenotyping for optimizing therapeutic capacity and ensuring the use of relevant cells in functional assays.
Article
Chemistry, Multidisciplinary
Mohammadrahim Kazemzadeh, Miguel Martinez-Calderon, Song Y. Paek, MoiMoi Lowe, Claude Aguergaray, Weiliang Xu, Lawrence W. Chamley, Neil G. R. Broderick, Colin L. Hisey
Summary: Placental extracellular vesicles (EVs) play important roles in pregnancy, but they are also implicated in the progression of preeclampsia. A fabrication process called laser-induced nano-structuring of SERS-active thin films (LINST) was developed to produce substrates that allow the biochemical fingerprinting of EVs. The findings encourage the exploration of surface-enhanced Raman spectroscopy (SERS) of EVs as a promising method for preeclampsia liquid biopsies.
Article
Chemistry, Analytical
Mohammadrahim Kazemzadeh, Miguel Martinez-Calderon, Weiliang Xu, Lawrence W. Chamley, Colin L. Hisey, Neil G. R. Broderick
Summary: Machine learning has significantly impacted the value of spectroscopic characterization tools, especially in biomedical applications. However, the extensive data preprocessing required often introduces bias. To address this, researchers developed two deep learning methods capable of fully preprocessing spectroscopy data without human input. The findings suggest that these methods outperform conventional approaches in terms of speed, defect tolerance, and classification accuracy.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Yourong Feng, Qi Chen, Sien Yee Lau, Bridget W. Tsai, Katie Groom, Carolyn J. Barrett, Lawrence W. Chamley
Summary: Placental extracellular vesicles (EVs) play a crucial role in feto-maternal communication and their uptake by recipient cells involves various mechanisms such as time, concentration, energy-dependence, as well as phagocytosis and endocytosis. The interaction between placental EVs and recipient cells is mediated by integrins.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Pharmacology & Pharmacy
Joy N. Reginald-Opara, Mingtan Tang, Darren Svirskis, Larry Chamley, Zimei Wu
Summary: This study investigates the impact of glutathione (GSH) on the transcellular transport mechanisms of liposomes through the blood-brain barrier (BBB). The presence of GSH increases the cellular uptake of liposomes in human brain microvascular endothelial cells and enhances the release of extracellular vesicles. GSH-PEG-L shows higher transport efficiency and brain distribution compared to PEG-L in in vitro and in vivo experiments.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2022)
Review
Medicine, Research & Experimental
Henry H. Louie, Odunayo O. Mugisho, Lawrence W. Chamley, Ilva D. Rupenthal
Summary: Extracellular vesicles (EVs) are cell-derived membrane vesicles that play important roles in intercellular communication and various physiological and pathological processes. Recent evidence suggests that EVs may also be involved in inflammatory eye diseases and could serve as novel biomarkers or therapeutics.
MOLECULAR PHARMACEUTICS
(2023)
Article
Materials Science, Biomaterials
Tianjiao Geng, Euphemia Leung, Lawrence W. Chamley, Zimei Wu
Summary: This study aimed to functionalize small extracellular vesicles (sEVs) with cyclic arginine-glycine-aspartic acid-tyrosine-cysteine (cRGDyC) to improve their targetability for glioblastoma (GBM) treatment. The cell targetability and intracellular trafficking of cRGDyC-sEVs were evaluated on U87 cells, showing a 2.4-fold enhancement in targetability compared to unmodified sEVs. Dox@cRGDyCsEVs exhibited superior cytotoxicity to U87 GBM cells compared to a standard liposome formulation.
BIOMATERIALS ADVANCES
(2023)
Article
Medicine, Research & Experimental
Matthew Kang, Colin Hisey, Bridget Tsai, Yohanes Nursalim, Cherie Blenkiron, Lawrence W. Chamley
Summary: Recently, researchers have focused on extracellular vesicles (EVs) as potential carriers for drug delivery, including gene therapy. However, current techniques used for EV generation have limitations in large-scale production. This study proposes that placental EVs produced from explant cultures are an efficient and safe method for EV production, and suggests that these EVs can be loaded with large plasmids. The study experimentally confirms successful plasmid loading into placental EVs using different loading strategies and provides insights into the loading efficiency dependent on DNA dose and size.
MOLECULAR PHARMACEUTICS
(2023)
Article
Nanoscience & Nanotechnology
Jesna Ashraf, Sandy Lau, Alireza Akbarinejad, Devon Bryant, Lawrence W. Chamley, Lisa I. Pilkington, David Barker, David E. Williams, Clive W. Evans, Jadranka Travas-Sejdic
Summary: Researchers have developed a simple and clean electrochemical method for the specific capture and fast release of nano-sized placental extracellular vesicles. This method can aid in understanding placental function and improve obstetric care.
ACS APPLIED NANO MATERIALS
(2023)
Article
Immunology
Paek Song, Brooks Anna, Graham Scott, Lawrence Willam Chamley
Summary: A study found that human placental extracellular vesicles (EVs) mainly interacted with macrophages in the spleen, liver, and lungs. However, there was little interaction between circulating leukocytes and EVs in vivo. Furthermore, there were differences in the interaction between EVs and leukocytes in pregnant and nonpregnant mice.
AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Xinyue Chen, Sofian Tijono, Bridget Tsai, Lawrence William Chamley, Lai-Ming Ching, Qi Chen
Summary: The biological connection between cancer and pregnancy is of interest because of the similar mechanisms between tumour and placental cells. Placental extracellular vesicles (EVs) may play a role in regulating the proliferation and invasion of placental cells, but the understanding of this regulation is limited. In this study, placental EVs were found to inhibit ovarian tumour growth in a xenograft model by inducing cellular necrosis and infiltrating CD169+ macrophages and NK cells into the tumour tissues.
BIOSCIENCE REPORTS
(2023)
Review
Cell Biology
Sien Yee Lau, Matthew Kang, Colin L. Hisey, Lawrence W. Chamley
Summary: Extracellular vesicles (EVs), lipid-bound vesicles released from cells, play a crucial role in many physiological processes and pathological mechanisms. Understanding their biodistribution is of great interest, and one accessible technology involves fluorescent labelling of exogenous EVs for whole-animal imaging. However, studying the fate of subcellular EVs requires adaptation of labelling techniques, excess dye removal and a refined experimental design.
DISEASE MODELS & MECHANISMS
(2023)
Meeting Abstract
Obstetrics & Gynecology
Cherry Sun, Katie M. Groom, Charlotte Oyston, Larry W. Chamley, Jo James
REPRODUCTIVE SCIENCES
(2023)
Meeting Abstract
Obstetrics & Gynecology
Anandita Umapathy, Jennifer Courtney, Toby Jackson, Mark Trew, Larry Chamley, Alys Clark, Helen Jones, Jo James
REPRODUCTIVE SCIENCES
(2023)
Review
Endocrinology & Metabolism
Claudia Main, Xinyue Chen, Min Zhao, Lawrence W. Chamley, Qi Chen
Summary: Multiparous women have a lower risk of developing ovarian and endometrial cancer, which has traditionally been attributed to the decrease in estrogen levels and increase in progesterone levels during pregnancy. However, recent evidence suggests that nonendocrine mechanisms may also play a role.
