Abnormal uterine artery remodelling in the stroke prone spontaneously hypertensive rat

Introduction: The stroke prone spontaneously hypertensive rat (SHRSP) is an established model of human cardiovascular risk. We sought to characterise the uteroplacental vascular response to pregnancy in this model and determine whether this is affected by the pre-existing maternal hypertension. Methods: Doppler ultrasound and myography were utilised to assess uterine artery functional and structural changes pre-pregnancy and at gestational day 18 in SHRSP (untreated and nifedipine treated) and in the normotensive Wistar-Kyoto (WKY) rat. Maternal adaptations to pregnancy were also assessed along with histology and expression of genes involved in oxidative stress in the placenta. Results: SHRSP uterine arteries had a pulsatile blood flow and were significantly smaller (70906 +/- 3903 mu m(2) vs. 95656 +/- 8524 mu m(2) cross-sectional area; p < 0.01), had a significant increase in contractile response (57.3 +/- 10.5 kPa vs 27.7 +/- 1.9 kPa; p < 0.01) and exhibited impaired endothelium dependent vasorelaxation (58.0 +/- 5.9% vs 13.9 +/- 4.6%; p < 0.01) compared to WKY. Despite significant blood pressure lowering, nifedipine did not improve uterine artery remodelling, function or blood flow in SHRSP. Maternal plasma sFLT-1/PlGF ratio (53 +/- 0.3 vs 4.6 +/- 0.1; p < 0.01) and the urinary albumin/creatinine ratio (1.9 +/- 0.2 vs 0.6 +/- 0.1; p < 0.01) was increased in SHRSP vs WKY. The SHRSP placenta had a significant reduction in glycogen cell content and an increase in Hif1 alpha, Sod1 and Vegf. Discussion: We conclude that the SHRSP exhibits a number of promising characteristics as a model of spontaneous deficient uteroplacental remodelling that adversely affect pregnancy outcome, independent of pre-existing hypertension. (C) 2015 The Authors. Published by Elsevier Ltd.