Article
Biotechnology & Applied Microbiology
Yanping Tan, Xiaolin Yang, Minghao Pei, Xin Xu, Chuntai Wang, Xinqiong Liu
Summary: The study aimed to investigate the regulation of gene expression in the interaction between rice and Magnaporthe oryzae using gene chip technology. Changes in gene expression at different time points post-inoculation were analyzed, revealing significant differentially expressed genes and identifying new genes related to rice blast. This research may contribute to the discovery of novel resistance genes and improve the understanding of rice blast development mechanisms.
Article
Biotechnology & Applied Microbiology
Gobong Choi, Jongbum Jeon, Hyunjun Lee, Shenxian Zhou, Yong-Hwan Lee
Summary: This study identified novel lncRNAs during host infection of the model pathogenic fungus Magnaporthe oryzae and revealed their potential roles in pathogenesis-related gene regulation.
Article
Plant Sciences
Junning Ma, Jean-Benoit Morel, Michael Riemann, Peter Nick
Summary: This study dissects the JA-dependent defense signaling in rice for both compatible and incompatible interactions by using JA-deficient mutants cpm2 and hebiba. The results demonstrate the pivotal role of JA in the basal immunity of rice in its resistance to the Rice Blast Fungus in both types of interactions.
Article
Plant Sciences
Gloria Escola, Victor M. Gonzalez-Miguel, Sonia Campo, Mar Catala-Forner, Concha Domingo, Luis Marques, Blanca San Segundo
Summary: In this study, a blast-resistant rice breeding line, COPSEMAR9, was obtained through hybridization and a high-quality genome sequence was obtained. The presence of rice blast resistance genes and defense-related genes, as well as miRNAs potentially contributing to blast resistance, were identified in COPSEMAR9.
Article
Biotechnology & Applied Microbiology
Huajun Zhu, Shenglian Wu, Shaojun Tang, Jun Xu, Yuelin He, Zuohua Ren, Erming Liu
Summary: In this study, cyclic lipopeptides extracted from Bacillus subtilis strain JN005 exhibited inhibition activity against the rice phytopathogenic fungus Magnaporthe oryzae. These isolated cyclic lipopeptides were tolerant to high temperature, extreme acidity, extreme alkalinity, and ultraviolet radiation. The growth of the mycelium and conidia of M. oryzae was abnormal after treatment with the cyclic lipopeptides.
BIOLOGICAL CONTROL
(2023)
Article
Plant Sciences
Guihua Duan, Chunqin Li, Yanfang Liu, Xiaoqing Ma, Qiong Luo, Jing Yang
Summary: This study utilized UHPLC/Q-TOF-MS to identify altered metabolites in transgenic rice infected with M. oryzae, revealing organic acids, organic oxygen compounds, lipids, and lipid-like molecules. The research showed that carbohydrate and amino acid metabolic pathways were involved in plant defense responses and resistance strengthening. The study also found that the identified metabolites could be utilized as an optimal marker for M.oryzae defense in rice.
Article
Plant Sciences
Huajun Zhu, Hu Zhou, Zuohua Ren, Erming Liu
Summary: Bacillus subtilis JN005 shows growth promotion and biocontrol efficacy against rice blast, improving germination and growth of rice seeds, enhancing defense enzyme activities in rice leaves, and reducing disease incidence by 79% and 76% in curative and preventive treatments, respectively. Additionally, field experiments demonstrate that spraying with JN005 suspension at 1x10(7) cfu/mL can effectively control rice blast and increase rice production yield.
JOURNAL OF PLANT GROWTH REGULATION
(2022)
Article
Biochemistry & Molecular Biology
Shang Gao, Yan Hou, Qiwei Huang, Pengzhi Wu, Zhikai Han, Danhong Wei, Huabin Xie, Fengwei Gu, Chun Chen, Jiafeng Wang
Summary: In this study, the important role of Osa-miR11117 in regulating rice defense mechanisms was investigated. It was found that Osa-miR11117 targets OsPAO4 to regulate blast resistance by adjusting polyamine metabolism and ethylene signaling pathways.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Microbiology
Jessie Fernandez, Victor Lopez, Lisa Kinch, Mariel A. Pfeifer, Hillery Gray, Nalleli Garcia, Nick Grishin, Chang-Hyun Khang, Kim Orth
Summary: The study identified two metalloproteinase enzymes, MoMca1 and MoMca2, in M. oryzae, which play significant roles in fungal growth and development, potentially regulating stress responses. The absence of these enzymes resulted in delays in fungal morphogenesis and insights into the mechanisms of M. oryzae pathogenicity.
Article
Microbiology
Ming-Hua Wu, Qin Yu, Tian-Yi Tao, Li-Xiao Sun, Hui Qian, Xue-Ming Zhu, Lin Li, Shuang Liang, Jian-Ping Lu, Fu-Cheng Lin, Xiao-Hong Liu
Summary: This study identified and analyzed the AGC kinases in Magnaporthe oryzae, a plant-pathogenic fungus. It revealed the important roles of AGC kinases in the growth, conidiation, germination, appressorium formation, and pathogenicity of the fungus. Furthermore, it discovered the specific functions of MoFpk1, a subfamily protein of AGC kinases, in cell wall integrity and lipid metabolism. The study also provided insights into the mechanisms of plasma membrane homeostasis and autophagy through lipid asymmetry maintenance.
Article
Agronomy
Dagang Tian, Zaijie Chen, Yan Lin, Tingmin Liang, Ziqiang Chen, Xinrui Guo, Feng Wang, Zonghua Wang
Summary: This study investigates the diversity, composition, and assembly of microbial communities associated with the rhizosphere soils and endosphere of Piz-t-KJ201 and NPB-KJ201. The host genotype and inoculation pattern of M.oryzae played dominant roles in determining the microbial community assemblage. Metabolomics analysis revealed that some metabolites may influence changes in bacterial communities.
Article
Agronomy
Na Cui, Xiaofeng Chen, Yan Shi, Meirong Chi, Jiantuan Hu, Kunlong Lai, Zonghua Wang, Haifeng Wang
Summary: DNA methylation plays a role in regulating the expression of coding and non-coding regions in plants. A study using whole-genome bisulfite sequencing revealed temporal DNA methylation changes in rice after infection with Magnaporthe oryzae, showing a slight increase in global DNA methylation levels compared to Arabidopsis. Over 38,000 differentially methylated regions (DMRs) were identified, with hypermethylated DMRs dominating over hypomethylated DMRs, most of which were located in transposable element regions. Transcription of NBS-LRR family genes was affected by changes in DNA methylation, indicating the essential role of DNA methylation in rice response to M. oryzae infection.
Article
Microbiology
Pengyun Huang, Huijuan Cao, Yan Li, Siyi Zhu, Jing Wang, Qing Wang, Xiaohong Liu, Fu-Cheng Lin, Jianping Lu
Summary: This study reveals the functional diversity and complexity of melanin in different strains of Magnaporthe oryzae. Melanin promotes spore production in the rice blast pathogen, but its effect is related to the background melanin content of the wild-type strains. Transcription factors like Cnf1 have opposite effects on conidiophore differentiation in different strains. Pig1 regulates melanin synthesis in hyphae but not in conidiophores, spores, or appressoria.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Plant Sciences
Vincent Ninkuu, Jianpei Yan, Lin Zhang, Zhenchao Fu, Tengfeng Yang, Shupeng Li, Beibei Li, Jiaqi Duan, Jie Ren, Guangyue Li, Xiufen Yang, Hongmei Zeng
Summary: This study investigated the mechanism of Hrip1-induced immunity in rice using transcriptome and metabolomic techniques. The results showed that Hrip1 activates key genes and molecular switches to enhance resistance against pathogens and increases the production of lignin and phytoalexins, thereby strengthening rice immunity.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Multidisciplinary Sciences
Romen Singh Naorem, Jochen Blom, Csaba Fekete
Summary: This study used comparative genome analysis to reveal genetic differences among clinical isolates of methicillin-resistant Staphylococcus aureus from Germany and Hungary, including variations in resistance and virulence genes.
Editorial Material
Plant Sciences
Ruyi Wang, Xiao Xu, Guo-Liang Wang, Yuese Ning
Summary: Dysfunction of susceptibility genes can lead to plant resistance to broad-spectrum resistance (BSR). A rice ubiquitin E3 ligase degrades the ortholog of a plant thermosensor to positively regulate blast resistance. Recent research also shows that this E3 ligase ubiquitinates a Ca2+ sensor to mediate rice BSR, expanding our understanding of susceptibility protein ubiquitination for plant BSR.
TRENDS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Fan Zhang, Hong Fang, Min Wang, Feng He, Hui Tao, Ruyi Wang, Jiawei Long, Jiyang Wang, Guo-Liang Wang, Yuese Ning
Summary: APIP5 plays dual roles in plants, acting as a transcription factor to regulate gene expression in the nucleus and as an RNA-binding protein to regulate mRNA turnover in the cytoplasm, a previously unidentified regulatory mechanism of plant transcription factors at the transcriptional and post-transcriptional levels.
NUCLEIC ACIDS RESEARCH
(2022)
Review
Plant Sciences
Jiyang Wang, Guojuan Xu, Yuese Ning, Xuli Wang, Guo-Liang Wang
Summary: Mitochondria play important roles in plant immunity by altering metabolism, producing reactive species, and triggering programmed cell death to defend against pathogen infection. Additionally, mitochondria coordinate with other organelles to integrate and amplify immune signals.
TRENDS IN PLANT SCIENCE
(2022)
Article
Cell Biology
Lauren S. Ryder, Neftaly Cruz-Mireles, Camilla Molinari, Iris Eisermann, Alice B. Eseola, Nicholas J. Talbot
Summary: Many plant pathogenic fungi infect their hosts using specialised cells called appressoria, which apply force or enzymes to break through the plant surface. Appressoria also suppress plant immunity and develop in response to physical characteristics of the leaf surface. The morphogenesis of appressoria is linked to cellular reorganization and cell wall remodelling.
JOURNAL OF CELL SCIENCE
(2022)
Review
Cell Biology
Iris Eisermann, Marisela Garduno-Rosales, Nicholas J. Talbot
Summary: Fungal pathogens undergo specific morphogenetic transitions to invade plants, and the ability to change cell shape is crucial for them. Septin GTPases play important roles in actin remodeling, plasma membrane curvature sensing, and cell polarity, and their assemblages are involved in cell shape changes and formation of infection structures in plant cells. This review focuses on the functions of septins in plant pathogenic fungi, particularly in invasive growth, and discusses their potential as targets for broad-spectrum antifungal plant protection strategies.
Article
Ecology
Richard J. Lindsay, Philippa J. Holder, Nicholas J. Talbot, Ivana Gudelj
Summary: A cornerstone assumption of classical virulence evolution theories, that pathogen growth rate is positively correlated with virulence, has been called into question by empirical evidence. By modifying a single metabolic gene, we engineered strains of Magnaporthe oryzae with different growth rates and conducted infection studies, revealing a non-monotonic relationship between growth rate and virulence. This novel framework provides a basis for developing new virulence evolution theories.
Article
Biochemistry & Molecular Biology
Xia Yan, Bozeng Tang, Lauren S. Ryder, Dan MacLean, Vincent M. Were, Alice Bisola Eseola, Neftaly Cruz-Mireles, Weibin Ma, Andrew J. Foster, Miriam Oses-Ruiz, Nicholas J. Talbot
Summary: The rice blast fungus is a major threat to global rice production. This study provides a detailed understanding of the biology behind the invasion of plant tissues during the disease. Through transcriptional profiling, the study identifies specific gene expression changes and a repertoire of effectors critical for successful infection.
Article
Biochemistry & Molecular Biology
Ely Oliveira-Garcia, Tej Man Tamang, Jungeun Park, Melinda Dalby, Magdalena Martin-Urdiroz, Clara Rodriguez Herrero, An Hong Vu, Sunghun Park, Nicholas J. Talbot, Barbara Valent
Summary: Fungi and oomycetes secrete effector proteins into living plant cells to suppress defenses and control plant processes. This study reveals that the blast fungus Magnaporthe oryzae packages cytoplasmic effectors into membranous compartments before translocating them into the plant cytoplasm. It demonstrates that clathrin-mediated endocytosis plays a crucial role in effector translocation, suggesting a manipulation of plant endocytosis by M. oryzae effectors.
Article
Multidisciplinary Sciences
Alejandra Martinez-D'Alto, Xia Yan, Tyler C. Detomasi, Richard I. Sayler, William C. Thomas, Nicholas J. Talbot, Michael A. Marletta
Summary: Blast disease caused by the fungus Magnaporthe oryzae leads to significant crop loss in cereal plants. The study shows that the expression of the putative polysaccharide monooxygenase MoPMO9A is increased during infection. MoPMO9A exhibits activity on cereal-derived mixed β-D-glucans and its domain architecture is different from other characterized AA9 PMOs. The results suggest that MoPMO9A plays a role in MBG degradation during plant infection.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Multidisciplinary Sciences
Lanlan Wang, Guojuan Xu, Lihua Li, Meiying Ruan, Anne Bennion, Guo-Liang Wang, Ran Li, Shaohong Qu
Summary: Receptor-like kinases (RLKs) in plants play diverse roles in plant development and stress responses by perceiving and transmitting environmental signals. In this study, a negative regulator named BDR1 was identified as a key player in rice resistance to the blast fungus Magnaporthe oryzae. BDR1 interacts with and phosphorylates MPK3, negatively regulating several defense-related pathways including jasmonate (JA) signaling and terpenoid biosynthesis. These findings contribute to our understanding of the molecular mechanisms underlying rice blast resistance.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Biochemistry & Molecular Biology
Sergio Latorre, Vincent Were, Andrew Foster, Thorsten Langner, Angus Malmgren, Adeline Harant, Soichiro Asuke, Sarai Reyes-Avila, Dipali Rani Gupta, Cassandra Jensen, Weibin M. Ma, Nur Uddin Mahmud, Md. Shabab Mehebub, Rabson Mulenga, Abu Naim Md. Muzahid, Sanjoy Kumar Paul, S. M. Fajle Rabby, Abdullah Al Mahbub Rahat, Lauren M. Ryder, Ram-Krishna Shrestha, Suwilanji R. Sichilima, Darren Soanes, Pawan Kumar Singh, Alison Bentley, Diane G. O. H. Saunders, Yukio Tosa, Daniel Croll, Kurt Lamour, Tofazzal J. Islam, Batiseba Tembo, Joe Win, Nicholas Talbot, Hernan A. Burbano, Sophien Kamoun
Summary: Wheat, a vital food crop, is being threatened by a pandemic of the blast disease. Research reveals that a clonal lineage of the wheat blast fungus has spread to Asia and Africa from South America. The lineage can be controlled by a disease resistance gene and fungicides, but there is a potential for the clone to evolve resistance to fungicides and sexually recombine with African lineages. Genomic surveillance is urgently needed to track and mitigate the spread of wheat blast and guide breeding for resistant varieties.
Editorial Material
Biochemistry & Molecular Biology
Vincent Were, Nicholas J. Talbot
Summary: Genome editing is a transformative technology that allows precise alteration of specific genes, enabling the development of disease-resistant crops. However, genetic modification has been more effective than genome editing in introducing new forms of disease resistance to crops.
Editorial Material
Plant Sciences
Xiaoman You, Yuese Ning, Guo-Liang Wang
TRENDS IN PLANT SCIENCE
(2023)
Article
Microbiology
Lauren S. Ryder, Sergio G. Lopez, Lucile Michels, Alice B. Eseola, Joris Sprakel, Weibin Ma, Nicholas J. Talbot
NATURE MICROBIOLOGY
(2023)
Article
Plant Sciences
Sanjoy Kumar Paul, Nur Uddin Mahmud, Dipali Rani Gupta, Kanistha Rani, Houxiang Kang, Guo-Liang Wang, Ljupcho Jankuloski, Tofazzal Islam
Summary: The study focused on investigating the cross-infection reactions of MoO and MoT in rice and wheat in Bangladesh. Results revealed that MoO pathotype can infect both wheat and rice plants, while MoT pathotype only infects wheat plants, indicating a potential wheat blast epidemic by MoO. Further research is needed to understand the variability in virulence of MoO and MoT isolates in infecting wheat and rice under controlled conditions.
PHYTOPATHOLOGY RESEARCH
(2022)