Editorial Material
Plant Sciences
Georgy A. Romanov, Thomas Schmuelling
Summary: The free bases of cytokinins are the biologically active forms of the hormone, while cytokinin ribosides function solely as transport forms without their own biological activity.
Article
Agronomy
Koji Nakanishi, Hiroko Fujiki, Koichi Ozaki, Satoko Yanahara, Naoko Takeuchi, Yuji Suzuki, Tamiji Sugiyama, Amane Makino, Taiichiro Ookawa, Tadashi Hirasawa
Summary: The study revealed that under soil moisture stress, the decrease in photosynthetic rate in rice leaves is mainly attributed to reduced cytokinin flux from roots to shoots and enhanced Rubisco degradation.
Article
Biochemistry & Molecular Biology
Kai Yang, Hongchun Sun, Mengxing Liu, Lingxiao Zhu, Ke Zhang, Yongjiang Zhang, Anchang Li, Haina Zhang, Jijie Zhu, Xiaoqing Liu, Zhiying Bai, Liantao Liu, Cundong Li
Summary: This study demonstrates that melatonin can delay drought-induced leaf senescence in cotton seedlings by enhancing the antioxidant enzyme system, improving photosynthetic efficiency, reducing chlorophyll degradation and ROS accumulation, and inhibiting ABA synthesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Horticulture
Xiaohua Ma, Qin Zhou, Qingdi Hu, Xule Zhang, Jian Zheng, Renjuan Qian
Summary: This study analyzed the effects of light intensity on Clematis tientaiensis and found that high light intensity caused chlorosis and reduced photosynthetic efficiency, while moderate light conditions resulted in good adaptability. The results highlight the importance of proper light management for the growth and population expansion of Clematis tientaiensis.
Article
Plant Sciences
Tengyu Li, Kun Luo, Chenlei Wang, Lanxin Wu, Jingwen Pan, Mingyang Wang, Jinwei Liu, Yan Li, Jinbo Yao, Wei Chen, Shouhong Zhu, Yongshan Zhang
Summary: In this study, a total of 27 GhCKXs genes were identified by genome-wide investigation and were distributed across 18 chromosomes. The gene structure and motif analysis showed that GhCKXs were remarkably conserved among the four groups, and cis-acting elements related to abiotic stress, hormones, and light response were found in their promoter regions. Transcriptome data and RT-qPCR analysis indicated that GhCKX genes had higher expression levels in various tissues and were involved in cotton's abiotic stress and phytohormone response. The protein-protein interaction network suggested that the CKX family may play a role in redox regulation and plant growth and development. Functionally, the GhCKX14 gene was found to improve drought resistance through modulating antioxidant-related activities via virus-induced gene silencing (VIGS).
Article
Agronomy
Yuting Li, Xuetao Han, Hao Ren, Bin Zhao, Jiwang Zhang, Baizhao Ren, Huiyuan Gao, Peng Liu
Summary: With global warming, high-temperature stress has become a major abiotic stress for crops, in particular summer maize in China. The effects of exogenous SA or 6-BA on growth, photosynthesis capacity, photosystem II activity, subcellular ultrastructure, antioxidant system, and plant hormones in maize leaves under HT stress were investigated.
Article
Environmental Sciences
Palak Bakshi, Pooja Sharma, Rekha Chouhan, Bilal Ahmad Mir, Sumit G. Gandhi, Renu Bhardwaj, Pravej Alam, Parvaiz Ahmad
Summary: This study aimed to assess the toxicity of Chlorpyrifos (CP) on Brassica juncea L. and investigate the ameliorative potential of phytohormone, 24-epi-brassinolide (EBL) mediated plant-microbe interaction in B. juncea L. The results showed that EBL and B2 treatment significantly increased the content of chlorophyll, carotenoids, flavonoids, anthocyanins, and xanthophylls in CP-stressed B. juncea seedlings. The supplementation of EBL and PGPR improved the expression of genes related to photosynthetic pigments and metabolic pathways. These findings suggest that EBL and PGPR can reduce CP-induced toxicity and promote recovery in B. juncea seedlings.
ENVIRONMENTAL POLLUTION
(2023)
Article
Plant Sciences
Nisma Farooq, Muhammad Omar Khan, Muhammad Zaheer Ahmed, Samia Fatima, Muhammad Asif Nawaz, Zainul Abideen, Brent L. Nielsen, Niaz Ahmad
Summary: Brassica juncea shows better tolerance to salt stress compared to Brassica napus, with higher photosynthetic potential and less ion accumulation. The reaction centers of PSII in B. juncea are more active and photoprotected, resulting in better efficiency and electron transport rates. Genetic differences, as reflected in the expression of key genes, contribute to the differential stress tolerance between these two Brassica species.
Article
Agriculture, Multidisciplinary
Dardan Klos, Michal Dusek, Erika Samol'ova, Marek Zatloukal, Vladimira Nozkova, Nasri Nesnas, Lenka Plackova, Radoslav Koprna, Zdenek Spisek, Hana Vylicilova, Ondrej Plihal, Karel Dolezal, Jiri Voller, Alena Kadlecova, Miroslav Strnad, Lucie Plihalova
Summary: In this study, four new cytokinin salts were synthesized and found to have higher water solubility compared to traditional cytokinins. Foliar treatment with these salts significantly reduced chlorophyll degradation and impairment of photosystem II functionality in barley leaf segments undergoing artificial senescence and maintained high concentrations of functional metabolites for several days.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Plant Sciences
Xiaoxia Yang, Yufen Che, Veder J. Garcia, Jianqiang Shen, Yutong Zheng, Zhezheng Su, Li Zhu, Sheng Luan, Xin Hou
Summary: Plants have developed various mechanisms to deal with different types of light stress, with the regulation of the electron transport chain being particularly important. The cytochrome b6/f complex plays a crucial role in regulating the electron transport chain under high light conditions. The study reveals that thylakoid-localized cyclophilin 37 (CYP37) maintains the activity of the cytochrome b6/f complex in Arabidopsis. The interaction between CYP37 and photosynthetic electron transfer A (PetA) indicates that CYP37 functions as a maintenance factor of the cytochrome b6/f complex rather than an assembly factor.
Article
Plant Sciences
Ningning Tong, Qingyan Shu, Baichen Wang, Liping Peng, Zheng'an Liu
Summary: This study investigated the developmental dynamics of tree peony annual shoots using anatomy, physiology, transcriptome, and metabolome analysis. The results showed that the developmental dynamics of the two cultivars were similar. The withering degree of P. suffruticosa 'Luoyanghong' was higher than that of P. ostii 'Fengdan'. Additionally, the study identified key genes and metabolic pathways related to the withering process of the annual shoots.
HORTICULTURE RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Violeta Velikova, Nia Petrova, Laszlo Kovacs, Asya Petrova, Dimitrina Koleva, Tsonko Tsonev, Stefka Taneva, Petar Petrov, Sashka Krumova
Summary: Single-walled carbon nanotubes (SWCNTs) have shown promising applications in biomedicine, pharmacology, and precision agriculture. Our study revealed that SWCNTs have a concentration-dependent effect on photosynthesis, with lower doses being beneficial for plant growth, while higher doses may impair photosynthetic function. Therefore, SWCNTs could be a potential nanocarrier for substances beneficial for plant growth in the future.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Lucy Lalthafamkimi, Paromik Bhattacharyya, Brijmohan Singh Bhau, Sawlang Borsingh Wann, Dipanwita Banik
Summary: The study presents a fast clonally stable regeneration protocol for Pogostemon cablin using the aromatic cytokinin meta-topolin (mT), addressing the challenges faced by wild populations. The research demonstrates genetic homogeneity of the regenerated clones and provides crucial insights for the high frequency regeneration of medicinally important plants like P. cablin.
SOUTH AFRICAN JOURNAL OF BOTANY
(2021)
Article
Plant Sciences
V. Kreslavski, A. Khudyakova, A. Kosobryukhov, P. Pashkovskiy, M. Vereshchagin, T. Balakhnina, H. F. Alharby, S. I. Allakhverdiev
Summary: The composition of light spectrum, acting through photoreceptors, is crucial for sustainable photosynthesis. In this study, the effects of cryptochrome 1 deficiency and additions of green light (GL) with different ratios of red light (RL) and blue light (BL) were investigated. The results showed that deficiency of cryptochrome 1 decreased photosynthesis rate, activity of photosystem II, and antioxidant enzyme activity, while increasing the content of oxidative products. However, the presence of additional GL offset these effects, possibly due to a decrease in active cryptochrome content.
Article
Cell Biology
Long Wang, Mu-Chun Wan, Ren-Yu Liao, Jie Xu, Zhou-Geng Xu, Hao-Chen Xue, Yan-Xia Mai, Jia-Wei Wang
Summary: Using time-resolved single-cell RNA sequencing, the cellular taxonomy of Marchantia polymorpha during asexual reproduction phases is defined. Two maturation and aging trajectories of the main plant body are identified: gradual maturation of tissues and organs along the tip-to-base axis of the midvein and progressive decline of meristem activities in the tip along the chronological axis. The observed aging trajectory is temporally correlated with the formation of clonal propagules, suggesting an ancient strategy to optimize resource allocation for producing offspring.
DEVELOPMENTAL CELL
(2023)
Review
Plant Sciences
Shubhpriya Gupta, Karel Dolezal, Manoj G. Kulkarni, Ervin Balazs, Johannes Van Staden
Summary: The application of biostimulants is an innovative and promising strategy to improve crop productivity by promoting seed germination. This technique is considered simple, beneficial, and sustainable. However, the mechanisms involved in regulating seed germination and seedling establishment by non-microbial biostimulants are still unclear.
PLANT GROWTH REGULATION
(2022)
Article
Plant Sciences
Mirella Sorrentino, Klara Panzarova, Ioannis Spyroglou, Lukas Spichal, Valentina Buffagni, Paola Ganugi, Youssef Rouphael, Giuseppe Colla, Luigi Lucini, Nuria De Diego
Summary: This study demonstrates how multivariate statistical analysis tools can be used to simplify omics data and extract relevant information in order to further understand the use of biostimulants in promoting plant growth and alleviating stress. The study found that lettuce and tomato plants exhibited different phenotypic and metabolomic responses to biostimulants, with biomass-related parameters being the most relevant traits to evaluate the biostimulant mode of action in lettuce, while chlorophyll fluorescence parameters and certain antistress metabolites were the relevant traits in tomatoes.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Jaroslav Nisler, Zuzana Pekna, Radka Koncitikova, Pavel Klimes, Alena Kadlecova, Nino Murvanidze, Stefaan P. O. Werbrouck, Lenka Plackova, David Kopecny, David Zalabak, Lukas Spichal, Miroslav Strnad
Summary: The study explores novel inhibitors of cytokinin degradation, which show increased selectivity and efficiency for agricultural and biotechnological applications. By investigating the structure-activity relationship of two series of CKX inhibitors, the researchers identified key substituents with enhanced selectivity for specific plant species and improved inhibitory activity. These inhibitors protect cytokinins from degradation and promote shoot regeneration in plants. Furthermore, the study also revealed clear differences in toxicity among the tested compounds.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Editorial Material
Plant Sciences
Shubhpriya Gupta, Johannes Van Staden, Karel Dolezal
PLANT GROWTH REGULATION
(2022)
Article
Plant Sciences
S. O. Amoo, N. A. Hlatshwayo, Karel Dolezal, J. O. Olowoyo
Summary: This study investigated the effects of temperature and photoperiod on seed germination of endangered Aloe species in South Africa, as well as the in vitro propagation of Aloe reitzii. The results showed that temperature and photoperiod significantly affected the germination speed and quality of the seeds, and the use of meta-topolin promoted shoot multiplication in Aloe reitzii.
SOUTH AFRICAN JOURNAL OF BOTANY
(2022)
Article
Materials Science, Multidisciplinary
Barbora Parizkova, Ioanna Antoniadi, David J. Poxson, Michal Karady, Daniel T. Simon, Marek Zatloukal, Miroslav Strnad, Karel Dolezal, Ondrej Novak, Karin Ljung
Summary: State-of-the-art technology based on organic electronics can be used for flow-free delivery of organic substances with high spatial resolution. The organic electronic ion pump (OEIP) can transport cytokinins to intact plants, allowing for targeted drug micro applications and regulation of physiological processes in plants.
ADVANCED MATERIALS TECHNOLOGIES
(2022)
Article
Plant Sciences
Alba E. Hernandiz, Carlos Eduardo Aucique-Perez, Sanja Cavar Zeljkovic, Nikola Stefelova, Sara Salcedo Sarmiento, Lukas Spichal, Nuria De Diego
Summary: Biostimulants are a popular area of research in alleviating the impact of abiotic stresses on crops. This study focused on small molecule-based biostimulants, specifically polyamines, and their effects on Arabidopsis seedlings under optimal growth conditions and stress. The results showed that priming with polyamines improved plant growth and stress tolerance, with specific metabolic pathways being implicated in regulating growth and development. This research highlights the potential of polyamines as effective biostimulants for mitigating the adverse effects of abiotic stress on plants.
Article
Plant Sciences
Chutarat Punchkhon, Panita Chutimanukul, Ratchata Chokwiwatkul, Triono Bagus Saputro, Aleel K. Grennan, Nuria De Diego, Lukas Spichal, Supachitra Chadchawan
Summary: This study investigated the functions of eight genes involved in photosynthesis under salt stress conditions using gene co-expression network analysis. The results showed that the expression of the LOC 0s01g68450 gene improved photosynthesis and salt tolerance in plants.
Correction
Agriculture, Multidisciplinary
Dardan Klos, Michal Dusek, Erika Samol'ova, Marek Zatloukal, Vladimira Nozkova, Nasri Nesnas, Lenka Plackova, Radoslav Koprna, Zdenek Spisek, Hana Vylicilova, Ondrej Plihal, Karel Dolezal, Jiri Voller, Alena Kadlecova, Miroslav Strnad, Lucie Plihalova
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Agriculture, Multidisciplinary
Dardan Klos, Michal Dusek, Erika Samol'ova, Marek Zatloukal, Vladimira Nozkova, Nasri Nesnas, Lenka Plackova, Radoslav Koprna, Zdenek Spisek, Hana Vylicilova, Ondrej Plihal, Karel Dolezal, Jiri Voller, Alena Kadlecova, Miroslav Strnad, Lucie Plihalova
Summary: In this study, four new cytokinin salts were synthesized and found to have higher water solubility compared to traditional cytokinins. Foliar treatment with these salts significantly reduced chlorophyll degradation and impairment of photosystem II functionality in barley leaf segments undergoing artificial senescence and maintained high concentrations of functional metabolites for several days.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Review
Plant Sciences
Nuria De Diego, Lukas Spichal
Summary: Commercial interest in biostimulants is increasing, along with the demand for efficient scientific methods to develop new products and understand their mechanisms of action. This review focuses on applying phenotyping approaches in biostimulant research and development, summarizing how phenotyping helped identify new biostimulants and describing their biological activity and mechanisms. High-throughput plant phenotyping can accelerate the development and characterization of new biostimulants.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Article
Biochemical Research Methods
Veronika Pilarova, Lukas Kuda, Hana Kocova Vlckova, Lucie Novakova, Shubhpriya Gupta, Manoj Kulkarni, Frantisek Svec, Johannes Van Staden, Karel Dolezal
Summary: The study successfully extracted quercetin from quince fruit using an environmentally friendly method, with optimized conditions of a CO2/ethanol/water ratio of 10/81/9, extraction temperature of 66 degrees Celsius, pressure of 22.3 MPa, and extraction time of 30 minutes. The method showed good repeatability and was applicable to various plant samples.
Article
Biochemistry & Molecular Biology
Dhekra Abdouli, Lenka Plackova, Karel Dolezal, Taoufik Bettaieb, Stefaan P. O. Werbrouck
Summary: The effects of mT and mTR on the proliferation and anomalies of Pistacia vera L. were studied and compared to that of BA. The results showed that mT had the highest proliferation rate and mTR had the lowest percentage of hyperhydric usable shoots and callus weight. However, leaf necrosis and shoot tip were not influenced by the different cytokinins. Image analysis revealed that BA and mTR had higher photosynthetic efficiency, but BA-treated shoots had higher anthocyanin accumulation, suggesting more stress. The study also investigated the metabolism of exogenous BA, mT, and mTR in pistachio.
Article
Biochemistry & Molecular Biology
Jana Bibova, Veronika Kabrtova, Veronika Vecerova, Zuzana Kucerova, Martin Hudecek, Lenka Plackova, Ondrej Novak, Miroslav Strnad, Ondrej Plihal
Summary: Plasmodiophora brassicae is a pathogen that causes clubroot disease in cruciferous plants, leading to morphological changes and symptoms such as gall formation and leaf abscission. The homeostasis of cytokinin and auxin appears to be crucial for club development. Study on late-stage infected Arabidopsis thaliana showed changes in cytokinin levels and their role in disease progression.
Article
Plant Sciences
Yuliya Krasylenko, Adela Hylova, Yevhen Sosnovsky, Marketa Ulbrichova, Lukas Spichal, Renata Piwowarczyk
Summary: This article investigates the seed dormancy and germination mechanisms of the obligate root parasite Cistanche armena, a rare and endangered species endemic to Armenia, and proposes a valuable tool for its conservation.
SEED SCIENCE RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Armando Moreno-Velasco, Mabel Fragoso-Serrano, Pedro de Jesus Flores-Tafoya, Sebastian Carrillo-Rojas, Elihu Bautista, Suzana Guimara Leitao, Jhon F. Castaneda-Gomez, Rogelio Pereda-Miranda
Summary: This study investigated the roots of Operculina hamiltonii, a traditional medicinal plant in Brazil. Three new acyl sugars or resin glycosides were identified and their structures elucidated. The combination of these compounds with other drugs showed enhanced cytotoxic effects on breast cancer cells.
Article
Biochemistry & Molecular Biology
Qian-Qing Liu, Lin-An Xiong, Jia-Yu Qian, Ting-Ting Gong, Lie-Feng Ma, Luo Fang, Zha-Jun Zhan
Summary: A new naturally occurring compound, Linderagatins C-F (1-4), with the structures of diaryltetrahydrofuran-type 7,9'-dinorlignans, was discovered in the roots of Lindera aggregata. The neuroprotective effects of compounds 2 and 3 on erastin-induced ferroptosis in HT-22 cells were found to be significant.