Article
Plant Sciences
Akihiro Saito, Kimika Hoshi, Yuna Wakabayashi, Takumi Togashi, Tomoki Shigematsu, Maya Katori, Takuji Ohyama, Kyoko Higuchi
Summary: The barley cultivar Sarab 1 can continue photosynthesis under low iron conditions through a reduction in P700 over-reduction and an increase in non-appressed thylakoid membranes. In contrast, another cultivar, Ehimehadaka-1, preferentially supplies iron to photosystem I to maintain photosynthetic activity. These findings highlight the multiple strategies that barley species have for acclimating photosynthesis to iron deficiency.
Article
Plant Sciences
Mithun Kumar Rathod, Sreedhar Nellaepalli, Shin-Ichiro Ozawa, Hiroshi Kuroda, Natsumi Kodama, Sandrine Bujaldon, Francis-Andre Wollman, Yuichiro Takahashi
Summary: Chlamydomonas reinhardtii's LHCs play a vital role in the function of photosystems. The protein Alb3.1 plays a key role in the assembly of LHCs, interacting with cpSRP43 and cpSRP54 proteins to form an assembly apparatus for most LHCs in the thylakoid membranes.
PLANT AND CELL PHYSIOLOGY
(2022)
Article
Mechanics
Mahdi Khatibi, Amirhosein Mojavezi, Esmaiel Pourjafarabadi
Summary: This study investigates the energy production characteristics of reverse electrodialysis (RED) in soft nanochannels, comparing the performance of conical and cylindrical geometries. The results demonstrate that conical nanochannels consistently outperform cylindrical nanochannels in terms of energy production efficiency. Furthermore, utilizing small concentration ratios and denser polyelectrolyte layers with distinct properties can enhance energy conversion efficiency.
Article
Biochemistry & Molecular Biology
Vaclav Svoboda, Hui Min Olivia Oung, Haniyeh Koochak, Robert Yarbrough, Steven D. Mckenzie, Sujith Puthiyaveetil, Helmut Kirchhoff
Summary: Knowledge of the abundance and ratio of photosynthetic protein complexes in thylakoid membranes is crucial for understanding energy conversion. This paper summarizes quantitative spectroscopic and biochemical methods for quantifying plant thylakoid protein complexes. It also compares mass spectrometric and classical protein quantification methods, revealing similar quantities of complexes and confirming the potential of mass spectrometry for thylakoid protein complex quantification.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2023)
Article
Plant Sciences
Long-Sheng Zhao, Chun-Yang Li, Xiu-Lan Chen, Qiang Wang, Yu-Zhong Zhang, Lu-Ning Liu
Summary: Visualization of the photosynthetic complexes in the thylakoid membranes of a cyanobacterium reveals their native architecture and interactions, providing insights into the organization and adaptation of the photosynthetic apparatus under environmental stress. This study sheds light on the molecular mechanisms of efficient light harvesting and energy conversion.
Article
Biochemical Research Methods
Eva Sarvari, Gabriella Gellen, Mate Sagi-Kazar, Gitta Schlosser, Katalin Solymosi, Adam Solti
Summary: In this study, the Blue/Clear-Native polyacrylamide gel electrophoresis (BN PAGE) method was improved by using a detergent mixture of 1% (w/V) n-dodecyl-beta-d-maltoside plus 1% (w/V) digitonin for solubilisation and 4.3-8% gel gradients for separation. Several large photosystem (PS) I containing bands were detected, and it was found that these bands were PSI-NADH dehydrogenase-like megacomplexes in maize bundle sheath thylakoids and PSI complexes with different light-harvesting complex (LHC) complements (PSI-LHCII, PSI-LHCII*) in mesophyll thylakoids of lincomycin treated maize. Proposed methods for quantitative determination and comparison of the complexes in different samples were also provided.
Article
Biophysics
Premashis Manna, Thomas Davies, Madeline Hoffmann, Matthew P. Johnson, Gabriela S. Schlau-Cohen
Summary: By studying the photophysical properties of LHCII embedded in different membranes, it was found that as the membrane area decreased, the conformational dynamics and quenched population increased. Additionally, membrane environments have the ability to regulate the conformation and photophysics of LHCII.
BIOPHYSICAL JOURNAL
(2021)
Article
Plant Sciences
Jayendra Pandey, Elsinraju Devadasu, Deepak Saini, Kunal Dhokne, Sureshbabu Marriboina, Agepati. S. S. Raghavendra, Rajagopal Subramanyam
Summary: This study found that the effects of drought on photosynthesis have been extensively studied, but the effects on thylakoid organization are limited. The research observed a significant decline in gas exchange parameters and a reduction of photochemical efficiency of photosystem II and I in pea leaves under progressive drought stress. Furthermore, the study found changes in the organization of light-harvesting complexes and reductions in supercomplexes of thylakoids in drought-affected plants.
Article
Plant Sciences
Iva Ilikova, Petr Ilik, Monika Opatikova, Rameez Arshad, Lukas Nosek, Vaclav Karlicky, Zuzana Kucerova, Pavel Roudnicky, Pavel Pospisil, Dusan Lazar, Jan Bartos, Roman Kouril
Summary: The study examined the structure-function relationship of PSII supercomplexes in Arabidopsis mutants to explore the possibility of spruce-type PSII supercomplex formation in angiosperms. Unlike in spruce, both LHCB3 and LHCB6 were found to be necessary for stable binding of the M trimer to the PSII core in Arabidopsis. The formation of spruce-type PSII supercomplex was observed with low abundance only in Ihcb3 plants, suggesting a unique structural arrangement in this mutant.
Article
Plant Sciences
Kunal Dhokne, Jayendra Pandey, Ranay Mohan Yadav, Pavithra Ramachandran, Jyoti Ranjan Rath, Rajagopal Subramanyam
Summary: Salt stress has adverse effects on the photosynthetic process and thylakoid structure in Pea. Under high salt conditions, the net photosynthetic rate decreases, water use efficiency improves, and the photochemical efficiency of both photosystems is reduced. Non-photochemical quenching is decreased, while non-regulated energy dissipation increases, leading to the inactivation of PSII. Salt stress also affects the electron transport chain, causing disorganization of thylakoid grana and altering the macro-organization and super complexes of thylakoids.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2022)
Article
Plant Sciences
Amy S. Verhoeven, Jennifer M. Berkowitz, Brenna N. Walton, Brandt K. Berube, Jerry J. Willour, Sidney B. Polich
Summary: Desiccation tolerant plants are able to accumulate zeaxanthin during dehydration, contributing to sustained energy dissipation, while sensitive plants do not. The phosphorylation of LHCII in tolerant mosses increases during desiccation, potentially enhancing connectivity between photosystems.
PHYSIOLOGIA PLANTARUM
(2021)
Article
Plant Sciences
Kyoko Higuchi, Keisuke Kurita, Takuro Sakai, Nobuo Suzui, Minori Sasaki, Maya Katori, Yuna Wakabayashi, Yuta Majima, Akihiro Saito, Takuji Ohyama, Naoki Kawachi
Summary: Iron is an essential element for plants, and their ability to acquire iron from low-iron solutions is not the sole determinant of tolerance to iron deficiency among barley genotypes. The live-autoradiography technique can help visualize the distribution of iron in plants and support phenotyping for nutrient transport.
Article
Plant Sciences
Vaclav Karlicky, Zuzana Kmecova Materova, Irena Kurasova, Jakub Nezval, Michal Stroch, Gyozo Garab, Vladimir Spunda
Summary: Light quality has a significant impact on plant metabolism, growth, and development. Recent research has shown that plants grown under monochromatic green light accumulate partially hydrogenated chlorophyll intermediates, affecting the structural-functional consequences of the photosynthetic apparatus. Accumulation of geranylgeranylated chlorophylls in pigment-protein complexes hampers the formation and stability of supercomplexes in thylakoid membranes, disrupting the photoprotective mechanism of non-photochemical quenching.
PHOTOSYNTHESIS RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Miao Chen, Frank Koopmans, Iryna Paliukhovich, Sophie J. F. van der Spek, Jian Dong, August B. Smit, Ka Wan Li
Summary: This study used an interaction proteomics workflow to reveal the existence of GABA(A) receptor subtypes containing both alpha 1 and alpha 6 subunits. Mass spectrometry analysis showed that these receptor subtypes exist in two main forms, with or without Neuroligin-2. Immunocytochemistry confirmed the presence of this synaptic GABA(A)R subtype at the synapse.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Olena Korotych, Thao T. Nguyen, Brandon C. Reagan, Tessa M. Burch-Smith, Barry D. Bruce
Summary: The study identified five highly efficient pSMA copolymers that can effectively extract membrane protein complexes from thylakoid membranes, showing promise in extracting MPCs from chloroplast thylakoids. Based on the differential extraction of protein complexes from spinach and pea thylakoids by pSMAs, the polymers can be divided into two groups: unfunctionalized polymers and ester derivatives.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2021)
Article
Biochemistry & Molecular Biology
Srilatha Nama, Sai Kiran Madireddi, Elsin Raju Devadasu, Rajagopal Subramanyam
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
(2015)
Article
Plant Sciences
Sireesha Kodru, Tirupathi Malavath, Elsinraju Devadasu, Sreedhar Nellaepalli, Alexandrina Stirbet, Rajagopal Subramanyam, Govindjee
PHOTOSYNTHESIS RESEARCH
(2015)
Letter
Biochemistry & Molecular Biology
Sangilimuthu Alagar Yadav, Daniel Pushparaju Yeggoni, Elsinraju Devadasu, Rajagopal Subramanyam
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2018)
Article
Plant Sciences
Sai Kiran Madireddi, Srilatha Nama, Elsinraju Devadasu, Rajagopal Subramanyam
PHOTOSYNTHESIS RESEARCH
(2019)
Article
Biochemistry & Molecular Biology
Sai Kiran Madireddi, Srilatha Nama, Elsin Raju Devadasu, Rajagopal Subramanyam
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
(2014)
Article
Plant Sciences
Elsinraju Devadasu, Dinesh Kumar Chinthapalli, Nisha Chouhan, Sai Kiran Madireddi, Girish Kumar Rasineni, Prabhakar Sripadi, Rajagopal Subramanyam
PHOTOSYNTHESIS RESEARCH
(2019)
Article
Biochemistry & Molecular Biology
Hari Parapatla, Ramurthy Gudla, Guruprasad Varma Konduru, Elsin Raju Devadasu, Hampapathula Adimurthy Nagarajaram, Manjula Sritharan, Rajagopal Subramanyam, Dayananda Siddavattam
BIOCHEMICAL JOURNAL
(2020)
Article
Biochemistry & Molecular Biology
Elsinraju Devadasu, Jayendra Pandey, Kunal Dhokne, Rajagopal Subramanyam
Summary: The eukaryotic alga Chlamydomonas (C.) reinhardtii is a model organism for studying photosynthetic efficiency. Under severe iron deficiency conditions, changes in thylakoid membrane organization and protein profiles affect photosynthetic efficiency, but supplementation with iron can partially restore the function of iron-deficient cells.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2021)
Article
Plant Sciences
Elsinraju Devadasu, Rajagopal Subramanyam
Summary: Under iron-limiting conditions, the unicellular alga Chlamydomonas reinhardtii accumulates significant amounts of triacylglycerols (TAGs) that are mainly derived from chloroplast lipids. The iron stress triggers rapid accumulation of TAGs, potentially contributing to efficient production of algal biofuels.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Nisha Chouhan, Elsinraju Devadasu, Ranay Mohan Yadav, Rajagopal Subramanyam
Summary: In this study, we investigated the lipid metabolism in Chlamydomonas reinhardtii cells under high light conditions. We found that a mutant strain, pgr5, had inhibited growth and increased lipid content compared to the wild-type strain. Further analysis revealed that pgr5 cells exhibited increased autophagy and triacylglycerol (TAG) accumulation, likely due to the upregulation of DGAT and PDAT enzymes. Our findings highlight the role of reactive oxygen species and autophagy in lipid production in microalgae.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Jayendra Pandey, Elsinraju Devadasu, Deepak Saini, Kunal Dhokne, Sureshbabu Marriboina, Agepati. S. S. Raghavendra, Rajagopal Subramanyam
Summary: This study found that the effects of drought on photosynthesis have been extensively studied, but the effects on thylakoid organization are limited. The research observed a significant decline in gas exchange parameters and a reduction of photochemical efficiency of photosystem II and I in pea leaves under progressive drought stress. Furthermore, the study found changes in the organization of light-harvesting complexes and reductions in supercomplexes of thylakoids in drought-affected plants.
Article
Plant Sciences
Elsinraju Devadasu, Sai Divya Kanna, Satyabala Neelam, Ranay Mohan Yadav, Srilatha Nama, Parveen Akhtar, Tamas F. Polgar, Bettina Ughy, Gyozo Garab, Petar H. Lambrev, Rajagopal Subramanyam
Summary: Salt stress induces a signaling mechanism that causes phosphorylation of LHCII mediated by Stt7, similar to light-induced state transitions. However, the phosphorylated LHCII does not attach to PSI but self-aggregates after detachment from PSII. Salt is a major stress factor in the growth of algae and plants. This study focuses on the long-term responses of Chlamydomonas reinhardtii to elevated NaCl concentrations and analyzes the physiological effects on the organization of the photosynthetic apparatus. The importance of LHCII phosphorylation in the acclimation and protection of the photosynthetic apparatus under salt stress is demonstrated. Rating: 8/10.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Multidisciplinary Sciences
Kiran-Kumar Shivaiah, Febri A. Susanto, Elsinraju Devadasu, Peter K. Lundquist
Summary: Plastoglobule lipid droplets are a dynamic sub-compartment of plant chloroplasts and cyanobacteria, playing a central role in the adaptation and remodeling of the thylakoid membrane. This article presents a rapid and effective method for isolating plastoglobules from plant leaf tissue and provides variations for isolating plastoglobules and related lipid droplet structures from different species.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2022)
