Review
Cell Biology
Nazli Alizadeh-Tabrizi, Stefan Hall, Christian Lehmann
Summary: Intravital microscopy is a unique imaging method that provides real-time insights into cellular functions without the need for tissue extraction. Despite specific challenges in lung imaging, advancements in surgical and optical techniques have enabled researchers to uncover mechanisms of leukocyte recruitment and function in models of pulmonary inflammation and infection.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Urology & Nephrology
Joana R. Martins, Dominik Haenni, Milica Bugarski, Marcello Polesel, Claus Schuh, Andrew M. Hall
Summary: The development of intravital imaging with multiphoton microscopy has revolutionized kidney research by allowing real-time visualization of dynamic cellular behavior and organelle function, providing insights into structure-function relationships in unprecedented detail. Advances in technology and image analysis have overcome practical challenges, opening up huge new possibilities for studying cellular mechanisms and disease states in the kidney.
KIDNEY INTERNATIONAL
(2021)
Article
Endocrinology & Metabolism
Melia D. Matthews, Emily Cook, Nada Naguib, Ulrich B. Wiesner, Karl J. Lewis
Summary: Osteocytes, as the resident mechanosensory cells in bone, play a crucial role in skeletal homeostasis and adaptation. This study demonstrates the usage of ultra-small and bright C'Dots as a novel in vivo imaging tool for osteocytes, which improves intravital imaging capabilities. It reveals distinct sex differences in nanoparticle intracellular dynamics and clearance in osteocytes and provides the first evidence of osteocyte integrin endocytosis and recycling in vivo. The results offer novel insights in osteocyte biology and open up new lines of investigation previously unavailable in vivo.
Review
Immunology
Philippe Bousso, Capucine L. L. Grandjean
Summary: Modulating the immune system can be used to treat various diseases and also improve our understanding of immune responses. This review discusses how imaging techniques can help clarify the mechanism of action of immunomodulatory strategies and guide the development of new drugs.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Mattia Sarti, Maria Parlani, Luis Diaz-Gomez, Antonios G. Mikos, Pietro Cerveri, Stefano Casarin, Eleonora Dondossola
Summary: This study developed an integrated computational pipeline based on an innovative variant of the U-Net neural network to segment and quantify cellular and extracellular structures of interest. The software shows promise for automatically detecting the elements of the FBR, unraveling the complexity of this pathophysiological process.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Oncology
Alexa R. Heaton, Peter R. Rehani, Anna Hoefges, Angelica F. Lopez, Amy K. Erbe, Paul M. Sondel, Melissa C. Skala
Summary: Metabolic reprogramming plays a significant role in tumorigenesis and cancer progression. However, current techniques for measuring tumor and immune cell metabolism lack spatial context. In this study, a mouse model and an algorithm for single cell imaging of immune and cancer cells were developed. The results showed metabolic changes in tumor infiltrating immune cells compared to immune cells from spleens, as well as single cell metabolic heterogeneity in both immune and tumor cells.
FRONTIERS IN ONCOLOGY
(2023)
Article
Neurosciences
Takahiro Takano, David I. Yule
Summary: The exocrine pancreas secretes fluid and digestive enzymes in response to parasympathetic release of acetylcholine and the gut hormone cholecystokinin. The secretion process is dependent on an increase in cytosolic calcium concentration. This study developed an in vivo method to observe pancreatic acinar calcium signals using a genetically expressed calcium indicator and multi-photon microscopy. The results showed that calcium signals in acinar cells are spatiotemporally regulated and disrupted in pathological conditions.
JOURNAL OF PHYSIOLOGY-LONDON
(2023)
Article
Biochemical Research Methods
Ahmad Shirazi, Tayebeh Sahraeibelverdi, Miki Lee, Haijun Li, Joonyoung Yu, Sangeeta Jaiswal, Kenn R. Oldham, Thomas D. Wang
Summary: We demonstrate a side-view dual axes confocal endomicroscope that can be repetitively inserted in hollow organs of genetically engineered mice for in vivo real-time imaging in both horizontal and vertical planes. Near infrared (NIR) excitation at λex = 785 nm was used, and a monolithic 3-axis parametric resonance scan mirror fabricated using micro-electromechanical systems (MEMS) technology enabled post-objective scanning in the distal end of a 4.19 mm diameter instrument. Torsional and serpentine springs were designed to switch the mode of imaging between vertical and horizontal planes by tuning the actuation frequency. This system achieved real-time in-vivo images with a depth of 310 μm and lateral and axial resolution of 1.75 and 7.5 μm, respectively, in both horizontal and vertical planes, allowing identification of individual cells and discrete mucosal structures.
BIOMEDICAL OPTICS EXPRESS
(2023)
Review
Immunology
Arpan Sharma Neupane, Paul Kubes
Summary: Highly dynamic immune responses involve multiple immune cell types working together to remove pathogens or injured tissue and return to homeostasis. Intravital microscopy (IVM) has been used to study the distinct immune cell behaviors in individual organs, showing that these behaviors can also influence each other. This review discusses how IVM has been applied to study innate immune responses in various tissues during homeostasis, injury, and infection.
IMMUNOLOGICAL REVIEWS
(2022)
Article
Physics, Applied
Kevin Keomanee-Dizon, Matt Jones, Peter Luu, Scott E. Fraser, Thai V. Truong
Summary: ExD-SPIM is an improved light-sheet microscopy strategy that solves the limitation of capturing fluorescence signals in large volumes by extending the depth of field (DOF) of high-NA detection objectives to match the thickness of the illumination light sheet. Compared to conventional light sheet imaging, ExD-SPIM improves the signal-to-noise ratio and reduces the rate of photobleaching. In whole-brain activity imaging, ExD-SPIM enhances signal sensitivity and volumetric coverage.
APPLIED PHYSICS LETTERS
(2022)
Article
Chemistry, Multidisciplinary
Johannes Rebling, Maya Ben-Yehuda Greenwald, Mateusz Wietecha, Sabine Werner, Daniel Razansky
Summary: A large-scale label-free optoacoustic microscopy approach has been developed for rapid and non-invasive imaging of tissue regeneration, providing new insights into angiogenesis and enabling automatic analysis of key features in wound angiogenesis. This approach is a versatile tool for preclinical research in tissue engineering and regenerative medicine, allowing for high-throughput and quantitative studies of vascular remodeling and response to pharmacological interventions in vivo.
Article
Multidisciplinary Sciences
Anoek Zomer, Davide Croci, Joanna Kowal, Leon van Gurp, Johanna A. Joyce
Summary: Tumors rely on continuous communication with their surrounding tissue and immune cells. By developing a multimodal imaging strategy, researchers discovered that the migratory behavior of tumor-associated macrophages varies in different genetically distinct glioblastomas and in response to targeted therapy. These findings highlight the importance of studying tumors longitudinally in vivo to understand the complex and dynamic changes in the tumor microenvironment during disease progression and treatment intervention.
Article
Chemistry, Multidisciplinary
Yingxian Zhang, Jincheng Zhong, Hui Cheng, Jie Huang, Zhenhui Li, Chi Zhang, Zhiang Gao, Zhourui Xu, Gaixia Xu, Ping Qiu, Ke Wang
Summary: By detailed measurements and comparison of the effective attenuation length (l(e)) in different excitation wavelengths, this study demonstrates that 3-photon microscopy at 1700 nm enables the deepest penetration in brain imaging in vivo.
NANOSCALE ADVANCES
(2023)
Review
Immunology
Juhee Pae, Johanne T. Jacobsen, Gabriel D. Victora
Summary: Germinal centers serve as the site for antibody affinity maturation, and multiphoton laser scanning microscopy has allowed insight into the dynamic biological processes within GCs. This technical advancement has greatly contributed to our understanding of GC biology.
IMMUNOLOGICAL REVIEWS
(2022)
Review
Cell Biology
Michael Mihlan, Shima Safaiyan, Manuel Stecher, Neil Paterson, Tim Laemmermann
Summary: Successful immune responses rely on the coordinated migration, interactions, and functioning of immune cells in lymphoid and parenchymal tissues. Real-time intravital microscopy has provided insights into the dynamic behavior of immune cells in living tissues, revealing unexpected biological features that were not observed in experiments outside of a living organism.
ANNUAL REVIEW OF CELL AND DEVELOPMENTAL BIOLOGY
(2022)